scholarly journals IDENTIFIKASI BAKTERI PADA SERASAH DAUN MANGROVE YANG TERDEKOMPOSISI DI BANDAR BAKAU KOTA DUMAI

2020 ◽  
Vol 7 (2) ◽  
pp. 167
Author(s):  
Putri Wening Ratrinia ◽  
Nirmala Efri Hasibuan ◽  
Aulia Azka ◽  
Sumartini Sumartini ◽  
Apri Mujiyanti ◽  
...  
Keyword(s):  
Leaf Litter ◽  
Bacterial Species ◽  
Mangrove Ecosystem ◽  
Morphological Observation ◽  
Plate Count ◽  
Survey Method ◽  
Mangrove Species ◽  
Gram Staining ◽  
Total Plate Count ◽  
Total Bacteria

Bacteria has an important role in decomposition and productivity activities in mangrove ecosystem. The study about identification of decomposing mangrove litter bacteria in Dumai has never been done. The aim of this study is to determine Total Plate Count and identify the dominant bacterial species from decomposing Rizhopora sp, Xylocarpus sp, dan Avicennia sp litter in Dumai. The method used in this research was survey method, bacterial isolation was done by pour plate method which is used in total calculation of bacteria was Total Plate Count (TPC), and identify the dominant bacterial in mangrove litter. The total bacteria which gained on Avicennia sp leaf litter are 0,64 x 105 Cfu/ml. On the other hand, the total bacteria from Rhizopora sp leaf litter has a lower number of colonies that is 0,55 x 105± 0,01 Cfu/ml. The bacterial identification test was carried out by gram staining method and morphological observation by microscopy. The dominant bacterial species in three mangrove species (Rizhopora sp, Xylocarpus sp, dan Avicennia sp) are Micrococcus sp dan Aerococcus sp. Keywords : Total Plate Count, Micrococcus sp, Aerococcus sp, Organic Matterial

scholarly journals KARAKTERISTIK KOMPOSISI KIMIA DAN TOTAL BAKTERI IKAN ROA (Hemirhampus sp.) ASAP YANG DI PASARKAN DI KECAMATAN BUNGKU TENGAH KABUPATEN MOROWALI

2020 ◽  
Vol 3 (1) ◽  
pp. 113
Author(s):  
Sarni Sarni ◽  
Haslianti Haslianti ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Chemical Composition ◽  
Random Sampling ◽  
Descriptive Analysis ◽  
Simple Random Sampling ◽  
Plate Count ◽  
Survey Method ◽  
Test Results ◽  
Total Plate Count ◽  
Total Bacteria ◽  
Treatment Water

Characteristics of Chemical Composition and Total Bacterial of Roa (Hemirhampus sp.) Smoke Marketed in the Bungku District of Central Morowali DistrictABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, and fat) and total bacteria (TPC) of smoked roa fish. Data analysis this study used descriptive analysis. The method used study is the survey method (Simple Random Sampling). The results of the chemical composition of the highest treatment water content P2 13.02%, ash P3 5.65%, protein P3 40.82%. and fat P3 13.87%. The best organoleptic test results were found in P1 with 7.30 appearance, 8.20 odor, 8.00 taste, 7.80 texture, and the best mushroom specifications were at P2 9.00. The lowest total bacteria test was found with 0,83×105 Log CFU/gr.Keywords: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia  (kadar air, kadar abu, kadar protein dan kadar lemak) dan total bakteri (TPC) terhadap ikan roa asap. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Metode yang digunakan dalam penelitian ini yaitu metode survey (Simple Random Sampling). Hasil penelitian komposisi kimia perlakuan tertinggi kadar air P2 13,02%. Kadar abu tertinggi terdapat pada perlakuan P3 5,65%. Kadar protein perlakuan tertinggi terdapat pada P3 40,82%. dan kadar lemak perlakuan tertinggi terdapat pada  P3 13,87%. Hasil penilaian uji organoleptik terbaik terdapat pada P1 sepesifikasi kenampakan 7,30, bau 8,20, rasa 8,00, tekstur 7,80 dan pada spesifikasi jamur nilai terbaik terdapat pada P2 9,00. Total bakteri terendah terdapat pada P3 dengan nilai 0,83×105 Log CFU/gr.Kata kunci: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik

scholarly journals CHARACTERISTICS AND FIGURES OF FRESH TILAPIA BACTERIA FROM TAMBAK SAWIYOH SIDOARJO

2020 ◽  
Vol 15 (1) ◽  
pp. 112
Author(s):  
Sara Wijimulyati ◽  
Erfan Andrianto Aritonang ◽  
Eka Rista Efrem Burga
Keyword(s):  
Bacterial Contamination ◽  
Positive Sample ◽  
Plate Count ◽  
Fresh Fish ◽  
Gram Staining ◽  
Total Plate Count ◽  
Water Media ◽  
The Media ◽  
Agar Media ◽  
Total Bacteria

ABSTRACTThis study aims to analyze characteristic and calculate the number of bacterial contamination of fresh nila fish from Sawiyoh pond Sidoarjo, East Java. A total of 30 fresh nila fish were taken and examined at the Microbiology Laboratory Faculty of Medicine Wijaya Kusuma Surabaya University. This research is a descriptive study with observational methods. The step of bacterial enrichment was carried out on BPW (Buffered Pepton Water) media. The bacterial identification procedure uses selective media SSA (Salmonella-Shigella Agar), Gram staining, and tests on TSIA media (Triple Sugar Iron Agar). The procedure for calculating the total bacteria using TPC (Total Plate Count) on NA (Nutrient Agar) media. The results on selective SSA media in 30 samples showed suspect Salmonella sp. with the characteristics of colorless colonies with black dots in the middle. The results of Gram staining in 30 samples showed that the bacteria were Gram negative in the form of rods. Test results on TSIA media identified one positive sample of Salmonella sp. (sample 09) with characteristics of the red slope of the media, the base of the media is yellow, and H2S is formed. The results of the calculation of total bacteria by the TPC method showed that the total bacterial total was 15.8 x 105 CFU / gram. The limit of microbial contamination according to the 2009 SNI for fresh fish is 5 x 105 CFU / gram so that fresh nila fish from Sawiyoh Sidoarjo pond has a number of bacterial contamination above the stipulated threshold.Keywords: Nila Fish, Gram Staining, Salmonella sp., Salmonella-Shigella Agar, Total Plate Count, Triple Sugar Iron Agar

scholarly journals The Existence test of Salmonella thypii and Eschericia coli of Corn Ice at Padang Bulan’s Traditional Market, Medan

2020 ◽  
Vol 6 (1) ◽  
pp. 96-102
Author(s):  
Toberni S. Situmorang
Keyword(s):  
Plate Count ◽  
Gram Negative Bacteria ◽  
Food Hygiene ◽  
E Coli ◽  
Gram Staining ◽  
Hygiene Factors ◽  
Existence Test ◽  
Descriptive Method ◽  
Total Bacteria

Food hygiene is an important factor to protect ourselves from the contamination of germs and bacteria that enter through these food intermediaries. Food contaminated by bacteria will cause disease and can cause death if not treated immediately. Common bacteria that contaminate food are Salmonella thypii and Eschericia coli. Both types of bacteria are a group of gram-negative bacteria in the form of bacilli. This study aims to analyze and identify the bacteria S. thypii and E. coli found in corn ice samples. The study was conducted with a descriptive method by conducting a bacteriological examination to determine the quality of sample cleanliness. There are 3 stages in this study, the first stage is calculation of amount of total bacteria (total plate count), analysis of S. thypii and E. coli bacteria and identification with Gram staining. The results showed 40% of samples tested is positive for S. thypii and 60% for E. coli. The largest amount of total bacteria was shown by samples 1 and 3, which were 78 cfu and 52 cfu, respectively. The presence of S. thypii and E. coli bacteria in the sample is thought to be due to poor hygiene factors in the processing of the drink

scholarly journals KONDISI WISATA MANGROVE DI DESA PASAR BANGGI, KABUPATEN REMBANG Mangrove Tourism Conditions in Pasar Banggi Village, Rembang Regency

2019 ◽  
Vol 8 (3) ◽  
pp. 221-225
Author(s):  
Danang Adi Saputro ◽  
Frida Purwanti ◽  
Siti Rudiyanti
Keyword(s):  
Species Composition ◽  
Mangrove Forest ◽  
Mangrove Ecosystem ◽  
Google Earth ◽  
Survey Method ◽  
Systematic Sampling ◽  
Sampling Techniques ◽  
Mangrove Species ◽  
Sampling Points ◽  
Exploratory Survey

ABSTRAK Mangrove merupakan tumbuhan yang hidup di daerah pasang surut sebagai ekosistem interface antara daratan dengan lautan. Ekosistem mangrove di desa Pasar Banggi Kabupaten Rembang merupakan perpaduan antara mangrove alami dan hasil rehabilitasi. Tujuan penelitian ini untuk mengetahui kondisi mangrove di Desa Pasar Banggi, Rembang dilihat dari  komposisi jenis, kerapatan dan ketebalan mangrove serta menganalisis tingkat kesesuaian wisata mangrove di Desa Pasar Banggi, Rembang. Metode yang digunakan adalah metode survey lapangan yang bersifat eksploratif, dimana  teknis pengumpulan data menggunakan sistematik sampling. Data yang diambil meliputi 5 variabel yaitu: jenis, kerapatan mangrove dan asosiasi biota (hasil pengamatan lapangan dan perbandingan dari penelitian terdahulu), ketebalan (citra Google Earth Oktober 2016), pasang surut (data BMKG Oktober 2016). Pengambilan sampel dilakukan pada 3 stasiun, dimana setiap stasiun terdapat 3 titik sampling. Komposisi jenis mangrove di desa Pasar Banggi terdapat 3 jenis mangrove yaitu Rhizopora stylosa, R. mucronata, dan R. Apiculata, dengan kerapatan mangrove tertinggi yaitu 62 ind/100m2 dan ketebalan mangrove tertinggi sepanjang 139 m. Kondisi hutan mangrove desa Pasar Banggi termasuk dalam kategori sesuai (S2) untuk kegiatan wisata berkelanjutan di Kabupaten Rembang. ABSTRACT Mangroves are plants that grow in a tidal areas an interface ecosystems between terrestrial and marine. Mangrove ecosystem in the Pasar Banggi Village,  Rembang Regency is a combination results of natural mangrove and rehabilitation. The purpose of this study were to determine condition of mangroves in the Pasar Banggi Village, Rembang, seen from the species composition, density and thickness of mangroves and to analyze the suitability level of mangrove tourism in the Pasar Banggi Village, Rembang. The method used in this study was an exploratory survey method, data collected using systematic sampling techniques. Mangrove tourism data collection was carried out of 5 variables, i.e.: type of mangrove, density of mangroves and associations of biota (from observations and comparisons of previous studies), thickness (Google Earth image October 2016), tides (data BMKG October 2016). Sampling was conducted at 3 stations, each station has 3 sampling points. The composition of mangrove species in Pasar Banggi village consists of 3 types of mangroves, namely Rhizopora stylosa, R. mucronata, and R. Apiculata, with the highest density of mangrove 62 ind / 100m2 and the highest thickness of mangrove along 139 m. The condition of mangrove forest in the Pasar Banggi village was included in the appropriate category (S2) for sustainable tourism activities in the Rembang Regency.

scholarly journals KARAKTERISTIK MUTU KIMIA DAN MIKROBIOLOGI GURITA (Octopus sp.) KERING YANG DIPASARKAN DIKABUPATEN KONAWE UTARA SULAWESI TENGGARA

2020 ◽  
Vol 3 (1) ◽  
pp. 120
Author(s):  
Asman Asman ◽  
Kobajashi Togo Isamu ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Chemical Composition ◽  
Random Sampling ◽  
Descriptive Analysis ◽  
Microbiological Quality ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Plate Count ◽  
Survey Method ◽  
Chemical Test ◽  
Total Plate Count

Chemical and Microbiological Quality Characteristics of Dried Octopus (Octopus sp.) Marketed in North Konawe Regency Southeast Sulawesi ABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, fat, and carbohydrate) and total bacteria (TPC) of dried Octopus. Data analysis of this study used descriptive analysis. The sampling technique of this study are used simple random sampling and used the survey method. The results of the highest chemical composition of the treatment was found at water content  S1 19.89%, ash S3 13.63%, protein S2 46.99%, fat S3 5.82% and carbohydrate S2 10.19%.  The best Total Plate Count (TPC) result was found at S2 3.8 × 104 Cfu/g Keywords: Chemical Test, Octopus (Octopus sp.) Dry, and Total Plate Count (TPC).ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia (kadar air, kadar abu, kadar protein, kadar lemak, dan kadar karbohidrat) dan total bakteri (TPC) terhadap gurita kering. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Teknik sampling yang digunakan pada penelitian ini adalah simple random sampling. Metode yang digunakan pada penelitian ini yaitu metode survei. Hasil penelitian komposisi kimia perlakuan tertinggi kadar air terdapat pada S1 19.89%, kadar abu tertinggi terdapat pada perlakuan S3 13.63%, kadar protein perlakuan tertinggi terdapat pada S2 46.99%, kadar lemak perlakuan tertinggi terdapat pada  S3 5.82% dan kadar karbohidrat perlakuan tertinggi terdapat pada S2 10.19%. Hasil Uji Total Plate Count (TPC) terendah terdapat pada S2 3.8×104 Cfu/g.Kata kunci: Gurita (Octopus sp.) kering, Total Plate Count (TPC) dan Uji Kimia.

scholarly journals Mangrove Ecosystem Suitability for Ecotourism Management Recommendation in Iboih Village – Sabang

2020 ◽  
Vol 151 ◽  
pp. 01060
Author(s):  
Cut Nelly ◽  
Saida Rasnovi ◽  
Zumaidar Zumaidar
Keyword(s):  
Mangrove Ecosystem ◽  
Survey Method ◽  
Mangrove Species ◽  
Marine Tourism ◽  
Suitability Index ◽  
Western Area ◽  
The Matrix ◽  
Avicennia Alba ◽  
Tourism Potential ◽  
Object Area

Iboih is one of the villages located in the western area of Weh Island which has been popular as the marine tourism potential. The mangrove ecotourism potential has not been well developed. This study aims to review the mangrove ecosystem suitability as ecotourism area in Iboih Village – Sabang. The methodology used was an explorative survey method. The method of potential suitability determination was scoring and weighting achieved from each parameter reviewed consisting of mangrove density, mangrove species, tidal, biota object, area characteristics, and accessibility. The output of the study found 3 (three) mangrove species including Rhizhophora mucronata, Rhizhophora apiculata, and Avicennia alba. There are supporting biota objects consisting of aquatic dan land fauna. Based on the matrix of potential suitability for mangrove ecotourism, it was found that the tourism suitability index (TSI) as 82.14% indicating that the mangrove ecosystem in Iboih is very suitable for ecotourism area. The benefit of the study is to provide a contribution to science and become the facilities for environmental education and conservation to the community.

Laju Dekomposisi Serasah Daun di Ekosistem Bakau Pulau Kelong, Kabupaten Bintan

2016 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
I Wayan Eka Dharmawan ◽  
Neviaty P. Zamani ◽  
Hawis H. Madduppa
Keyword(s):  
Leaf Litter ◽  
Decomposition Rate ◽  
Substrate Surface ◽  
Environmental Parameters ◽  
Mangrove Ecosystem ◽  
Mangrove Forests ◽  
Mangrove Species ◽  
Southeastern Part ◽  
Regional Productivity ◽  
High K

<strong>Decomposition Rate of Leaf Litter in The Mangrove Ecosystem of Kelong Island, Bintan Regency.</strong> The mangrove forests in the southeastern part of Kelong Island have abundant carbon reserves that indicate high primary productivity areas. Decomposition of leaf litter, as a part of regional productivity, produces simple essential nutrients utilized to sustain the growth of mangroves. The purpose of this study was to determine the decomposition rate of leaf litter in each zone of the study, the Landward (L), Middlezone (M), and Seaward (S) which is distinguished by the abundance of plant species and in situ environmental parameters. The experiment was conducted in March–May 2015 in the mangrove areas of Island Kelong, Bintan regency. The decomposition process was observed at six time intervals, i.e. 2, 4, 7, 14, 28, and 42 days using net bag containing 10 g of wet leaves placed on the substrate surface. The results showed that L was a zone with highest percentage of decomposition (56.34 ± 20.58%) compared to the M and S, respectively for 51.59 ± 15.46% and 51.09 ± 8.70%. The highest rate of litter decomposition in zone L was 1.40 ± 0.82 g·m-2·day-1 that was significantly different from the zones M and S, respectively of 0.83 ± 0.89 g·m-2·day-1 and 0.78 ± 0.70 g·m-2·day-1.  Analysis of the coefficient of decomposition rate (k) indicated that the rate of decomposition in this region was high (k &gt; 0.01). The decomposition rate has a significant correlation with the abundance of mangrove species, the salinity value of the water, and soil temperature.

scholarly journals KARAKTERISTIK BAKTERI ASAM LAKTAT YANG DIISOLASI DARI SUSU KUDA SUMBA

2019 ◽  
Vol 7 (1) ◽  
pp. 85-92
Author(s):  
Annytha Detha
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Selective Medium ◽  
Plate Count ◽  
Gram Positive ◽  
Negative Results ◽  
Gram Staining ◽  
Total Plate Count ◽  
Agar Media ◽  
Veterinary Laboratory

The purpose of this study was to isolate and identify the lactic acid bacteria found in Sumba mares milk. This research will be carried out from September to November 2017 at the Veterinary Laboratory of the Livestock Service Office of East Nusa Tenggara Province. The research stages are isolation of lactic acid bacteria from Sumba mares milk by growing lactic acid bacteria on MRS agar media, and identifying lactic acid bacteria with gram staining, catalase test, motility test, and total plate count testing. Based on the results of the study, the presence of lactic acid bacteria originating from Sumba mares milk was characterized by the presence of colonies of lactic acid bacteria from Sumba mares milk which grew on MRS media agar as a selective medium for lactic acid bacteria. Based on the results of testing the characteristics of lactic acid bacteria Sumba mares milk has Gram positive, the negative results in the catalase test are characterized by not forming gas bubbles when the bacteria are added with H2O2. Based on the motility test obtained negative results or bacteria are non-motile and the number of lactic acid bacteria that can be a total picture of lactic acid bacteria that exist in Sumba mares milk in milliliter of milk. The conclusion are lactic acid bacteria isolated from Sumba mares milk has characteristic as Gram positive, basil or stem shaped, negative catalase and non motile, and has a total lactic acid bacteria of 3.5 x 108 cfu/ml (est).      

scholarly journals Identifikasi Sakarin, Siklamat, dan Natrium Benzoat Serta Karakteristik Susu Kedelai yang Dijual di Pasar Tradisional Wilayah Jimbaran, Bali Selama Penyimpanan

2021 ◽  
Vol 10 (1) ◽  
pp. 141
Author(s):  
Novelita Olivea Herman ◽  
Ni Luh Ari Yusasrini ◽  
I Nengah Kencana Putra
Keyword(s):  
Sodium Benzoate ◽  
Sampling Technique ◽  
Plate Count ◽  
Survey Method ◽  
Soy Milk ◽  
Sensory Assessment ◽  
Traditional Markets ◽  
Milk Samples ◽  
Total Plate Count

This study aims to identify saccharin, cyclamate, and sodium benzoate in soy milk circulating in traditional markets in the Jimbaran region, determine the percentage of soy milk samples in traditional markets in the Jimbaran region that contain saccharin, cyclamate, and sodium benzoate, and determine the characteristic of milk soybeans in the traditional markets of the Jimbaran region, Bali during storage. This study uses a survey method with saturation sampling technique. Saccharin, cyclamate and sodium benzoate were analyzed qualitatively and quantitatively while the characteristic of soy milk were observed included pH, total plate count, and sensory assessment. Saccharin, cyclamate, sodium benzoate, pH, total plate count, and sensory assessment were analyzed using descriptive methods. The results showed that soy milk in traditional markets in the Jimbaran region did not contain saccharin, 80% used cyclamate, 100% used sodium benzoate, and characteristics of soy milk in traditional markets in Jimbaran, Bali during 8 hours storage has pH between 6.8 to 8.34, as much as 70% of soy milk has total plate count less than 106 CFU/ml, and it is still well consuming because the color has the like criteria, aroma and flavor has the neutral criteria and very not acidic.

scholarly journals Quantification of Litter Fall and Estimation of Nutrient Release Through in-Situ Decomposition of Leaf Litter From Some Important Mangrove Species of Indian Sundarbans

2021 ◽  
Author(s):  
S.K. Das ◽  
Ranjan Kumar Manna ◽  
Pranab Gogoi ◽  
Roshith C. M. ◽  
Sajina A. M. ◽  
...  
Keyword(s):  
Carbon Sequestration ◽  
Leaf Litter ◽  
Nutrient Dynamics ◽  
Nutrient Release ◽  
Mangrove Ecosystem ◽  
Litter Fall ◽  
Mangrove Species ◽  
Indian Sundarbans ◽  
In Situ Experiments

Abstract Looking into the importance of mangrove leaf litter in regulation of sediment carbon sequestration and nutrient flux in Sundarbans ecosystem, an experiment was conducted at Jharkhali island of Sundarbans. In this experiment, collection of leaf litter-shedding from nine dominant mangrove species during December 2012 to November 2013 was donemonthly using ‘litter traps’ (1 m2) in Eco-garden on the bank of Herobhanga creek.Seasonal litter fall was highest in Geon (Excoecariaagallocha)(103 gm-2) followed by Keora (Sonneratiaapetala) (98.5 gm-2). Kal Bain (Avicennia alba) produced the highest amount (414.37 gm-2) of total annual leaf litter followed by Bruguieragymnorrhiza (410.43 gm-2). Kankra (Bruguieragymnorrhiza), Garjan (Rhizophoramucronata) and Geon (Excoecariaagallocha) dry leaf litters contained more than 50% carbon (oven dry basis). Litter from Avicennia group contained more nitrogen and carbon. Decomposition rates of various mangrove litters were estimated through twoshort-term (30 days and 52 days) in-situ experiments using mangrove leaf litter in nylon net bags (0.6 mm mesh) subjected to periodical diurnal submergence by tidalriver water at Jharkhali. During decomposition process,observation said thatmost susceptible and resistant litter with respect to mass loss were Geon (Excoecariaagallocha) (81±5.5%) and Taura (Aegialitisrotundifolia) (26±4%) respectivelyafter 30 days. The biomass retained after decomposition losses (average45±15.2%after 30 days and 56±20.2 % after 52 days) indicated the amount of carbon retained in mangrove soil and ultimately determines the carbon sequestered in soil through mangrove litter fall. The study gives important insight into contribution of different mangrove species in carbon sequestration and nutrient dynamics in mangrove ecosystem of Indian Sundarbans.

Sign in / Sign up

Export Citation Format

Share Document