scholarly journals Evaluasi toksisitas Madu hasil produksi lebah yang diberi pakan tambahan ekstrak air Moringa oleifera terhadap larva Artemia salina

2021 ◽  
Vol 7 (1) ◽  
pp. 112-122
Author(s):  
Aliyah Aliyah ◽  
Veni Hadju ◽  
Muhammad Dasir ◽  
Muhammad Raihan
Keyword(s):  
Moringa Oleifera ◽  
Artemia Salina

Madu dan Moringa oleifera merupakan salah satu bahan alam yang memiliki nilai nutrisi dan potensi sebagai bahan obat yang cukup besar. Penelitian ini bertujuan untuk mengevaluasi toksisitas madu hasil ternak lebah yang diberi pakan sirup sukrosa dengan suplementasi M. oleifera menggunakan Artemia salina sebagai hewan model. Komponen madu kelor (MK) dan M. oleifera mula-mula dianalisis dengan menggunakan sistem HPTLC dengan fase gerak terdiri dari n-heksana dan etil asetat dengan perbandingan 2:1. Toksisitas MK kemudian diuji menggunakan A. salina sebagai model organisme dengan konsentrasi antara 10 hingga 2000 μg / ml dalam media air laut buatan. Tingkat kematian dilaporkan dalam persentase A. salina jumlah larva yang mati terhadap total larva yang diuji. Nilai LC50 dihitung dengan menggunakan analisis probit. Hasil analisis pada penelitian ini menunjukkan bahwa beberapa komponen M. oleifera dapat dideteksi pada MK berdasarkan nilai Rf yang diperoleh dalam analisis HPTLC. Selanjutnya, berdasarkan nilai LC50 dalam penelitian ini, MK dianggap sebagai bahan dengan toksisitas yang rendah. Namun, penelusuran lebih lanjut pada komponen fitokimia yang bertanggung jawab pada toksisitas ini perlu dilakukan untuk melihat korelasinya dengan toksisitas MK

scholarly journals Estudo toxicológico das sementes de Moringa oleifera Lam. utilizando o teste com Artemia salina Leach

2020 ◽  
Vol 9 (9) ◽  
pp. e457997332
Author(s):  
Danilo Lima Dantas ◽  
Ana Paula Moisés de Sousa ◽  
Jaciara Dantas Costa ◽  
José Anderson Machado Oliveira ◽  
Arthur Filgueira de Almeida ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Artemia Salina ◽  
Moringa Oleifera Lam

A busca por espécies vegetais, sobretudo aquelas que apresentam maiores benefícios econômicos e também sociais vem ganhando ênfase nas pesquisas científicas. Nesse contexto destaca-se a Moringa oleífera Lam., planta originária da Índia e que apresenta diversas aplicações nos setores alimentícios, industriais, medicinais, tratamento de água, dentre outros. Seus frutos são constituídos de vagens que contêm sementes ricas em minerais, vitaminas, proteínas, lipídios, carboidratos, metabólitos secundários e antioxidantes. O presente trabalho teve por objetivo fazer um estudo toxicológico das sementes da M. oleifera Lam. a partir do teste da Artemia salina Leach. Foram preparados dois extratos etanólicos, sendo um a partir das amêndoas e outro apenas com as cascas que as recobrem. Os extratos foram solubilizados em 5 concentrações diferentes. Cada tubo de ensaio continha os extratos em suas respectivas concentrações e 10 A. salina ativas. Após 24 h foi realizada a contagem para determinação da CL50. Os dados obtidos foram analisados a partir de análise PROBIT com uso do software Statistica. A partir das análises realizadas concluiu-se que a nem a amêndoa e nem a casca da semente da M. oleifera Lam. apresentavam toxicidade, uma vez que ambas tiveram valores de CL50 acima de 1000 µg.mL-1, revelando inclusive uma menor toxicidade que outras plantas convencionalmente utilizadas no Brasil, como o endro e a erva doce.

scholarly journals UJI TOKSISITAS EKSTRAK AKUADES (SUHU KAMAR) DAN AKUADES PANAS (70 oC) DAUN KELOR (Moringa oleifera Lamk.) TERHADAP LARVA UDANG Artemia salina Leach

ALCHEMY ◽  
2014 ◽  
Author(s):  
Syaiful Anwar ◽  
Eny Yulianti ◽  
Abdul Hakim ◽  
Ahmad Ghanaim Fasya ◽  
Begum Fauziyah ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Artemia Salina

<p>Kelor (<em>Moringa oleifera</em> Lamk.) adalah tanaman yang kaya nutrisi. Kandungan nutrisi tersebar pada seluruh bagian tanaman kelor, mulai dari daun, kulit batang, bunga, buah (polong), sampai akarnya. Kelor (<em>Moringa oleifera</em> Lamk.) mempunyai banyak manfaat, misalnya digunakan sebagai koagulan, nutrisi, vitamin, dan sebagai obat. Selama ini kelor di Indonesia hanya digunakan sebagai tanaman pagar dan sayuran  dan masih jarang ada penelitian tentang bioaktivitas daun kelor dan pemanfaatannya  sebagai antikanker. Penelitian ini mempelajari toksisitas ekstrak akuades (suhu kamar) dan akuades panas (70 <sup>o</sup>C) daun kelor (<em>Moringa oleifera</em> Lamk.) terhadap larva udang Artemia salina Leach dengan menggunakan metode BSLT. Pemilihan pelarut akuades sangat menguntungkan karena ekonomis dan mudah diperoleh, sehingga mudah diaplikasikan oleh masyarakat.Hasil penelitian menunjukkan bahwa ekstrak akuades (suhu kamar) dan akuades panas (70 <sup>o</sup>C) daun kelor memiliki tingkat toksisitas terhadap Artemia salina Leach yang ditunjukkan dengan nilai LC<sub>50</sub> kurang dari 1000 ppm. Ekstrak akuades panas (70 <sup>o</sup>C) memiliki toksisitas lebih baik dari pada ekstrak akuades (suhu kamar) karena dihasilkan nilai LC<sub>50</sub> berturut-turut 163,979 ppm dan 265,977 ppm. Kandungan golongan senyawa pada ekstrak akuades panas (70 <sup>o</sup>C) adalah alkaloid, flavonoid, tanin dan triterpenoid. Berdasarkan hasil tersebut bahwa daun kelor mempunyai potensi sebagai tanaman sediaan herbal yang nantinya bisa dimanfaatkan sebagai antibakteri dan antikanker.</p>

scholarly journals Caracterização físico-química, toxicológica e nutricional das folhas da Moringa oleifera Lam secas e in natura

2019 ◽  
Vol 8 (11) ◽  
pp. e178111450
Author(s):  
Gabriel Barbosa Câmara ◽  
Tharcia Kiara Beserra de Oliveira ◽  
Celenia de Souto Macedo ◽  
Daniela Dantas de Farias Leite ◽  
Tamires da Cunha Soares ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Artemia Salina ◽  
Moringa Oleifera Lam

O presente estudo teve como objetivo elaborar extratos, seco e in natura, provenientes das folhas da M. oleífera, bem como realizar as caracterizações físico-químicas e toxicológicas. Inicialmente, os extratos foram confeccionados a partir das técnicas de secagem e homogeneização das folhas da M. oleifera, estando estas nos estados seco ou in natura. Logo em seguida, as amostras foram caracterizadas quanto aos seguintes parâmetros: teor de água (Umidade), atividade de água, cinzas, lipídios, teor de sólidos solúveis totais, acidez titulável total, pH, e vitamina C. Foi realizado o teste de toxicologia por Artemia salina Leach, que têm como objetivo avaliar os efeitos tóxicos em sistemas biológicos e prever a possibilidade da toxicidade de substâncias frente a outros sistemas.  Os resultados das análises físico-químicos encontrados para as folhas in natura e seca da M. oleifera foram, respectivamente: Teor de água (Umidade) de 73,38 e 5,49 g/100 g, AW foi de 0,89 e 0,43 g/100g, cinzas de 2,53 e 8,14 g/100 g, lipídios de 1,27% e 6,87%, o SST foi de 3,03 e 4,9º Brix, ATT de 0,55 e 1,95 g/100 g, pH de 5,7 e 5,68 e vitamina C de 1036,323 e 365,26mg/100 g. Para a toxicidade frente Artemia sp. observou uma DL50 de 12,734 mg/kg para as folhas da M. oleifera in natura e 5,925 mg/kg para as folhas secas, onde ambas são consideradas atóxicas. Diante disto, conclui-se que as folhas in natura e secas da M. oleifera são uma alternativa interessante, viável e de baixo custo para a alimentação e suplementação humana, podendo ser aplicada em diversos seguimentos, visto que apresentaram parâmetros físico-químicos e toxicológicos bastante satisfatórios, atendendo aos valores determinados pelas legislações vigentes, bem como, resultados semelhantes a diversos estudos na área.

scholarly journals Antibacterial Activity and Cytotoxicity of Three Lectins Purified from Drumstick (Moringa oleifera Lam.) Leaves

1970 ◽  
Vol 17 ◽  
pp. 89-94 ◽  
Author(s):  
Shahanaz Khatun ◽  
MMH Khan ◽  
M Ashraduzzaman ◽  
Farzana Pervin ◽  
Luthfunnessa Bari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Antibacterial Activities ◽  
Artemia Salina ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Plant Materials ◽  
Gram Negative

Context: Plant materials contain glycoproteins (phytolectins) that are toxic in nature may play a key role in the control of various normal and pathological processes in living organisms and have diverse biochemical and diagnostic applications. Objectives: Screening of three lectins SLL-1, SLL-2 and SLL-3 purified from Drumstick (Moringa oleifera Lam.) leaves for their antibacterial activities and brine shrimp lethality bioassay. Materials and Methods: Three bioactive lectins were purified from Drumstick leaves by conventional chromatographic methods. The lectins were tested for their antibacterial activities against three pathogenic bacteria- Escherichia coli (gram-negative) Shigella dysenteriae (gram-negative) and Staphylococcus aureus (gram-positive) using the standard disc-diffusion method. Mortality of the brine shrimp naupli was assessed by hality bioassay. Results: All the lectins showed antibacterial activity against E. coli, Sh. dysenteriae and St. aureus. They also showed cytotoxic effect in brine shrimp (Artemia salina L.) lethality bioassay. The LC50 values of SLL-1, SLL-2 and SLL-3 were found to be 15.8, 17.78 and 14.12 μg/ml respectively. The experimental results revealed that SLL-3 is more cytotoxic than other lectins. The lectin SLL-3 showed lowest activity whereas SLL-1 showed highest activity against the three bacteria. Conclusion: Results suggest that the extracts from M. oleifera leaf can be a source of natural antimicrobials with potential applications in pharmaceutical industry to control coliform bacteria.Key words:  Drumstick; Moringa oleifera; lectins; antibacterial activity; brine shrimp; bioassayDOI: 10.3329/jbs.v17i0.7112J. bio-sci. 17: 89-94, 2009

scholarly journals Assessment of toxicity of Moringa oleifera flower extract to Biomphalaria glabrata, Schistosoma mansoni and Artemia salina

Chemosphere ◽  
2015 ◽  
Vol 132 ◽  
pp. 188-192 ◽  
Author(s):  
Cláudio A.A. Rocha-Filho ◽  
Lidiane P. Albuquerque ◽  
Luanna R.S. Silva ◽  
Patrícia C.B. Silva ◽  
Luana C.B.B. Coelho ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Moringa Oleifera ◽  
Biomphalaria Glabrata ◽  
Artemia Salina ◽  
Flower Extract

scholarly journals Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine

2012 ◽  
Vol 72 (3) ◽  
pp. 505-509 ◽  
Author(s):  
DDR. Arcanjo ◽  
ACM. Albuquerque ◽  
B. Melo-Neto ◽  
LCLR. Santana ◽  
MGF Medeiros ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Ageratum Conyzoides ◽  
Bioactive Natural Products ◽  
Flower Extract ◽  
The Mean ◽  
Ethanol Extracts

The brine shrimp (Artemia salina Leach) lethality bioassay offers an advantage in standardization and quality control of botanical products. This test is well correlated with antitumor activity (cytotoxicity) and can be used to monitor the activity of bioactive natural products. This paper reports the bioactivity of ethanol extracts from seven medicinal plants from the Northeast of Brazil (Acmella uliginosa, Ageratum conyzoides, Eugenia uniflora, Plectranthus neochilus, Moringa oleifera, Justicia pectoralis and Equisetum sp.) against Artemia salina. Biological activity was evaluated for extracts at 1, 10, 100, and 1000 µg/mL in triplicate, and the mean lethal concentration values (LC50) were obtained by probit analysis. The species Acmella uliginosa showed the highest bioactivity, and its flower extract was more active than its leaf extract.

Structural similarity of ribosomes from E. coli and A. salina

1978 ◽  
Vol 36 (2) ◽  
pp. 242-243
Author(s):  
M. Boublik ◽  
R.M. Wydro ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Ribosomal Proteins ◽  
Direct Evidence ◽  
Structural Similarity ◽  
Carbon Support ◽  
Artemia Salina ◽  
Uranyl Acetate ◽  
Biological Assays ◽  
E Coli ◽  
And Function ◽  
Fine Carbon

Ribosomes are ribonucleoprotein particles necessary for processing the genetic information of mRNA into proteins. Analogy in composition and function of ribosomes from diverse species, established by biochemical and biological assays, implies their structural similarity. Direct evidence obtained by electron microscopy seems to be of increasing relevance in understanding the structure of ribosomes and the mechanism of their role in protein synthesis.The extent of the structural homology between prokaryotic and eukaryotic ribosomes has been studied on ribosomes of Escherichia coli (E.c.) and Artemia salina (A.s.). Despite the established differences in size and in the amount and proportion of ribosomal proteins and RNAs both types of ribosomes show an overall similarity. The monosomes (stained with 0.5% aqueous uranyl acetate and deposited on a fine carbon support) appear in the electron micrographs as round particles with a diameter of approximately 225Å for the 70S E.c. (Fig. 1) and 260Å for the 80S A.s. monosome (Fig. 2).

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

Evaluation of Lubricating Performance of Biodegradable Moringa Oleifera Oil

2016 ◽  
Vol 2 (08) ◽  
pp. 530-536 ◽  
Author(s):  
M. H Mosarof ◽  
M. A Kalam ◽  
H. H Masjuki ◽  
A. M Ashraful
Keyword(s):  
Moringa Oleifera ◽  
Moringa Oleifera Oil
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