scholarly journals Antibacterial Activity and Cytotoxicity of Three Lectins Purified from Drumstick (Moringa oleifera Lam.) Leaves

1970 ◽  
Vol 17 ◽  
pp. 89-94 ◽  
Author(s):  
Shahanaz Khatun ◽  
MMH Khan ◽  
M Ashraduzzaman ◽  
Farzana Pervin ◽  
Luthfunnessa Bari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Antibacterial Activities ◽  
Artemia Salina ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Plant Materials ◽  
Gram Negative

Context: Plant materials contain glycoproteins (phytolectins) that are toxic in nature may play a key role in the control of various normal and pathological processes in living organisms and have diverse biochemical and diagnostic applications. Objectives: Screening of three lectins SLL-1, SLL-2 and SLL-3 purified from Drumstick (Moringa oleifera Lam.) leaves for their antibacterial activities and brine shrimp lethality bioassay. Materials and Methods: Three bioactive lectins were purified from Drumstick leaves by conventional chromatographic methods. The lectins were tested for their antibacterial activities against three pathogenic bacteria- Escherichia coli (gram-negative) Shigella dysenteriae (gram-negative) and Staphylococcus aureus (gram-positive) using the standard disc-diffusion method. Mortality of the brine shrimp naupli was assessed by hality bioassay. Results: All the lectins showed antibacterial activity against E. coli, Sh. dysenteriae and St. aureus. They also showed cytotoxic effect in brine shrimp (Artemia salina L.) lethality bioassay. The LC50 values of SLL-1, SLL-2 and SLL-3 were found to be 15.8, 17.78 and 14.12 μg/ml respectively. The experimental results revealed that SLL-3 is more cytotoxic than other lectins. The lectin SLL-3 showed lowest activity whereas SLL-1 showed highest activity against the three bacteria. Conclusion: Results suggest that the extracts from M. oleifera leaf can be a source of natural antimicrobials with potential applications in pharmaceutical industry to control coliform bacteria.Key words:  Drumstick; Moringa oleifera; lectins; antibacterial activity; brine shrimp; bioassayDOI: 10.3329/jbs.v17i0.7112J. bio-sci. 17: 89-94, 2009

scholarly journals Antibacterial Activities of Xanthium Strumarium L.

2018 ◽  
Vol 29 ◽  
pp. 70-77 ◽  
Author(s):  
Anjana Devkota ◽  
Ritu Kumari Das
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Xanthium Strumarium ◽  
Distilled Water ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. The antibacterial activity was studied against six pathogenic bacteria, three gram negative: Klebsiella pneumoniae (ATCC 15380), Proteus mirabilis (ATCC 49132), Escherichia coli (ATCC 25922) and three gram positive: Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25932) at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ ml, 250 mg/ml) of leaf extracts of X. strumurium. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antibacterial activity of extracts was determined by disc diffusion method and zone of inhibition (ZOI) was measured. Gram negative bacteria was found more resistant than gram positive bacteria. The most susceptible bacterium was S. aureus while the most resistant bacterium was E. coli. Methanolic extract was found more effective than distilled water. These findings suggest that extracts obtained from leaves of X. strumurium possess biobactericidal potential, which can suitably be exploited for making antibacterial drugs.J. Nat. Hist. Mus. Vol. 29, 2015, Page: 70-77

scholarly journals Antibacterial and Cytotoxic Activity of Longiverbenone Isolated from the Rhizome of Cyperus scariosu

1970 ◽  
Vol 25 (1) ◽  
pp. 82-84 ◽  
Author(s):  
M Shafiqur Rahman ◽  
M Nural Anwar
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Chromatographic Technique

Longiverbenone is a naturally occurring sesquiterpene isolated from ethanolic extract of Cyperus scariosus rhizome by solvent-solvent portioning and chromatographic technique. The antibacterial activity of longiverbenone was evaluated against eleven potential human pathogenic bacteria using disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth macrodilution method. Cytotoxic activity (lethal concentration 50%, LC50) of longiverbenone was determined on new borne brine shrimp (Artemia salina). Longiverbenone showed moderate to good antibacterial activity against the test organisms tested herein. It exhibited the lowest MIC (20 μg/ml) and MBC (80 μg/ml) against Vibrio cholerae. The LC50 of the isolated sesquiterpene was found to be 14.38 μg/ml against new borne brine shrimp. Keywords: Antibacterial activity; Cytotoxic activity (LC50); Cyperus scariosu; Longiverbenone; Minimum inhibitory concentration (MIC)DOI: http://dx.doi.org/10.3329/bjm.v25i1.4866 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 82-84

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

scholarly journals Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

2021 ◽  
Vol 8 (3) ◽  
pp. 24-28
Author(s):  
Puteri Amelia ◽  
Rachma Ayunda ◽  
Saiful Bahri
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacillus Subtilis Atcc

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

scholarly journals PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF TOKAR SHA AGAINST ENTERIC BACTERIA

2021 ◽  
Vol 5 (1) ◽  
pp. 436-444
Author(s):  
Sabiu Shitu ◽  
M. Attahiru ◽  
F. A. Iliya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Enteric Bacteria ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
North West ◽  
E Coli ◽  
Enteric Infections

The antibacterial activity of Tokar sha; a local traditional medication widely used by many people in North-west zone of Nigeria especially Sokoto, Kebbi and Zamfara against enteric infections were examined against some clinical isolates of pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella typhi) using agar well diffusion method. The pattern of inhibition varied with the tokar sha concentrations and the organisms tested. The tokar sha was more effective on E. coli with a maximum zone of growth inhibition of 25mm at 35mg/ml followed by B. cereus (20mm). However, S. aureus and S. typhi were resistant to tokar sha at all concentrations tested. The minimum inhibitory concentrations (MIC) were found to be 35mg/ml for both E. coli and B. cereus. The antibacterial activities exhibited by tokar sha in this study could be attributed to the presence of its constituents which signifies the potential of the tokar sha as a therapeutic agent. These findings may justify the ethnomedicinal use of tokar sha as an antibacterial agent against enterobacteria

scholarly journals Sponge-Associated Actinobacteria: Morphological Character and Antibacterial Activity against Pathogenic Bacteria

2017 ◽  
Vol 3 (1) ◽  
pp. 21-26
Author(s):  
SASMIATI FARACH DITA ◽  
SRI BUDIARTI ◽  
YULIN LESTARI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Marine Ecosystem ◽  
Morphological Characteristics ◽  
Antibacterial Activities ◽  
Morphological Characters ◽  
Antimicrobial Activities ◽  
Shigella Dysenteriae ◽  
Rrna Gene

Sponge-associated actinobacteria may diverse and have potency to produce bioactive compounds. Diversity and antimicrobial activity of indigenous sponge-associated actinobacteria isolated from the marine ecosystem in Indonesia have not much been explored. This work aimed to assess morphological and antibacterial activity of sponge-associated actinobacteria. The morphological characteristics were examined based on their color of aerial and substrate mycelia, and pigmentation, while antibacterial activities were assayed using the antagonist technique. The selected actinobacterial isolate was identified using 16S rRNA gene. Various sponge-associated actinobacteria were successfully isolated from Hyrtios sp., Callyspongia sp., and Neofibularia sp. sponges. A total of 62 actinobacterial isolates were obtained, and each isolate showed a variety of morphological characters, which could be seen in aerial mass color, substrate mass color, and pigmentation. Actinobacterial isolates were tested against human pathogenic bacteria, i.e. Staphylococcus aureus and Methicillin-Resistant S. aureus, representing Gram-positive, and Escherichia coli EPEC K1-1 and Shigella dysenteriae, representing Gram-negative. Most of actinobacterial isolates had antimicrobial activities at least against one of pathogenic bacteria. High activity was shown by NOHa.2, isolated from Neofibularia, and HRHa.5 isolated from Hyrtios. The NOHa.2 showed the highest antimicrobial activity against S. dysenteriae, meanwhile, HRHa.5 showed antimicrobial activity against 3 of 4 tested bacterial pathogens. These data showed diversity of sponge-asccociated actinobacteria from marine ecosystem in Indonesia, and several of them have potency as source of antibacterial compounds

scholarly journals Biological activities of essential oils and methanol extracts of five Ocimum species against pathogenic bacteria

2013 ◽  
Vol 31 (No. 2) ◽  
pp. 195-202 ◽  
Author(s):  
S. Saha ◽  
T.D. Dhar ◽  
C. Sengupta ◽  
P. Ghosh
Keyword(s):  
Essential Oils ◽  
Antibacterial Activities ◽  
Ocimum Basilicum ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Gram Negative ◽  
Methanol Extracts ◽  
Ocimum Gratissimum ◽  
Ocimum Species ◽  
Pharmaceutical Industries

The essential oils and methanol extracts of Ocimum basilicum L., Ocimum kilimandscharicum Guerke, Ocimum gratissimum L, Ocimum canum Sims, and Ocimum tenuiflorum L. (green type) were examined for their potential antibacterial activities. The chemical composition of essential oils of Ocimum species was analysed by GC-MS. The inhibitory effects of essential oils and methanol extracts were studied on two Gram-positive (Bacillus subtilis, Micrococcus luteus) and five Gram-negative (Pseudomonas aeruginosa, Shigella dysenteriae, Escherichia coli, Vibrio cholera, and Shigella flexneri) bacteria using disc-diffusion method. Minimum inhibitory concentration (MIC) was assessed by micro broth dilution method. The antibacterial test results showed that the essential oils of Ocimum basilicum L., Ocimum kilimandscharicum Guerke, and Ocimum gratissimum L. strongly inhibited the growth of all of the microorganisms studied, especially of the Gram-negative strains, whereas other two essential oils showed moderate activities. The result may suggest that the essential oils of Ocimum possess compounds with antibacterial activities, and therefore could be used as natural preservative ingredients in food and/or pharmaceutical industries.  

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

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