scholarly journals Development of chemoregulatory method for monitoring grape pests

2021 ◽  
pp. 253-259
Author(s):  
Н.В. Алейникова ◽  
Я.Э. Радионовская ◽  
Л.В. Диденко ◽  
В.В. Андреев ◽  
В.Э. Глебов ◽  
...  
Keyword(s):  
Biological Activity ◽  
Plant Protection ◽  
Average Rate ◽  
Lobesia Botrana ◽  
Halyomorpha Halys ◽  
Pheromone Traps ◽  
Stink Bug ◽  
Stink Bugs ◽  
Entomological Research ◽  
European Grape Moth

В практике защиты растений одним из актуальных методов мониторинга насекомых является хеморегуляторный метод, к средствам которого относят феромоны, гормоны, аттрактанты, репелленты и т.д., предназначенные для выявления фитофагов и оценки уровня заселенности ими сельскохозяйственных культур. Цель исследований заключалась в проведении сравнительных испытаний новых и усовершенствованных феромонных ловушек и препаратов для мониторинга доминирующего вредителя винограда - гроздевой листовёртки ( Lobesia botrana Den. et Schiff.) и инвазийного карантинного вида - коричнево-мраморного клопа ( Halyomorpha halys Stаl.) на виноградниках Крыма. Работу проводили в условиях 2020 г. согласно методическим подходам, используемым в отечественной и международной практике энтомологических исследований на промышленных насаждениях основных зон виноградарства Крыма. Установлено, что при средней интенсивности лёта бабочек I генерации гроздевой листовёртки на винограднике сорта Каберне-Совиньон опытные феромонные ловушки типа «Дельта», «Ромб» и «Квадро» производства ФГБУ «ВНИИКР» показали близкие значения уловистости: в среднем 826-1042 экз./ловушку. Наиболее перспективной определена ловушка «Дельта». На фоне средней и низкой интенсивности лёта бабочек I-III генераций гроздевой листовёртки на виноградниках трех зон установлен одинаковый уровень биологической активности четырех феромонных препаратов производства АО «Щелково Агрохим» (разница 0,3-3,1 %) как на фольгапленовых диспенсерах (1,5 мг феромона), так и на трубчатых (0,8-3,0 мг феромона). Показан достаточный уровень эффективности фольгапленовых диспенсеров на протяжении четырех месяцев (снижение биологической активности 4-14 %). На фоне низкой численности коричнево-мраморного клопа на виноградниках установлена более высокая (в 5,2 раза) биологическая активность препарата с феромоном и аттрактантом синтеза АО «Щелково Агрохим» в сравнении с препаратом, содержащем только феромон. Использование этих феромонных препаратов в ловушках барьерного типа позволило впервые выявить новый карантинный вид клопа на участках двух зон виноградарства Крыма. One of the most relevant methods for monitoring insects in the practice of plant protection is the chemoregulatory method, the means of which are pheromones, hormones, attractants, repellents, etc., intended to identify phytophages and assess the level of population density on agricultural crops. The aim of the research was to carry out comparative tests of new and improved pheromone traps and preparations for monitoring the dominant grape pest - European grape moth ( Lobesia botrana Den. Et Schiff.) and the invasive quarantine species - brown marmorated stink bug ( Halyomorpha halys Stаl.) in the vineyards of Crimea. The work was carried out in the conditions of 2020 according to the methodological approaches used in national and international practices of entomological research on industrial plantations of the main viticulture zones of Crimea. It was found that with an average flight intensity of butterflies of European grape moth of the I generation in the vineyard of ‘Cabernet-Sauvignon’ cultivar, experimental pheromone traps of Delta, Romb and Quadro types produced by FSBI VNIIKR showed similar values of catching capacity: at the average rate of 826-1042 nos. / trap. Delta was defined as the most promising trap. Against the background of an average and low flight intensity of European grape moth butterflies of the I-III generations in the vineyards of three zones, similar level of biological activity of four pheromone preparations produced by JSC Shchelkovo Agrokhim (difference - 0.3-3.1%) was established on propylene foil (1, 5 mg of pheromone) and tube dispensers (0.8-3.0 mg of pheromone). A sufficient level of efficiency of propylene foil dispensers was shown during four months (a decrease in biological activity by 4-14%). Against the background of a low number of brown marmorated stink bugs in the vineyards, a higher (5.2 times) biological activity of the preparation with pheromone and attractant of JSC Shchelkovo Agrokhim production in comparison with the preparation containing only pheromone was established. Using of these pheromone preparations in barrier-type traps made it possible to reveal for the first time a new quarantine bug species in the plots of two Crimean viticultural zones.

Effect of Trap Color and Residual Attraction of a Pheromone Lure for Monitoring Stink Bugs (Hemiptera: Pentatomidae)

2020 ◽  
Vol 55 (4) ◽  
pp. 437-447
Author(s):  
Ted E. Cottrell ◽  
Rammohan R. Balusu ◽  
Edgar Vinson ◽  
Bryan Wilkins ◽  
Henry Y. Fadamiro ◽  
...  
Keyword(s):  
Native Species ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Stink Bugs ◽  
Euschistus Servus ◽  
Trap Color ◽  
Residual Efficacy ◽  
Conducting Research ◽  
Multiple Species ◽  
Research Studies

Abstract Stink bugs (Hemiptera: Pentatomidae) are commonly monitored using pyramid traps baited with a pheromone. Initially, the pyramid traps were painted yellow and predominantly used to monitor native stink bug species. However, research studies involving the exotic Halyomorpha halys Stål (Hemiptera: Pentatomidae) now use pyramid traps that are black, not yellow. As H. halys moves across the southeastern United States, the use of a single trap, yellow or black, for monitoring and conducting research studies would be beneficial. Our objective was to compare black and yellow pyramid traps baited with a lure to determine if one was superior for trapping herbivorous stink bugs. This study was conducted at four locations, three in Alabama and one in Georgia, over 2 yr. Additionally, residual efficacy of the lure was measured via trap capture over 1-mo intervals. Our results showed that only when native stink bug species were combined, and only in 1 yr, were captures significantly affected by trap color. Capture of the exotic H. halys and the most abundant native species, Euschistus servus (Say), was not significantly affected by trap color. Trap capture was significantly affected by how long a lure was in a trap. The data from this study suggests that when traps are used in conjunction with a pheromone to monitor multiple species of adult stink bugs, especially native species, the yellow pyramid trap is favored.

scholarly journals Biological control agents of the number of Halyomorpha halys Stål

2021 ◽  
Vol 22 (4) ◽  
pp. 561-569
Author(s):  
Irina S. Agasyeva ◽  
Maria V. Nefedova
Keyword(s):  
Essential Oils ◽  
Plant Protection ◽  
Central Zone ◽  
Field Tests ◽  
Federal State ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Krasnodar Krai ◽  
Experimental Preparation

Research aimed at studying the control of the number of pests of many agricultural crops – Halyomorpha halys Stål, was carried out during 2018-2020 on the territory of the Central zone of Krasnodar Krai. In the experiments, there were used individuals of a brown marmorated stink bug, caught by hand and using a pheromone trap at different stations (tree and shrub vegetation, soybean crops). In 2020, the dynamics of the abundance of the brown marmorated stink bug was monitored since the end of May (the time of the appearance of the first individuals of H. halys) to the third decade of October. Among the natural entomophages in Krasnodar Krai, two species of parasitic insects were found: Pediobius cassidae Erdos. (Hymenoptera: Eulophidae) and Anastatus bifasciatus Geoffroy (Hymenoptera: Eupelmidae), infecting about 5-10 % of H. halys eggs in the field, which did not significantly affect the abundance of the brown marmorated stink bug. In the laboratory, essential oils from several botanical families were tested: Umbelliferae, Compositae, Abies. Essential oils of wormwood, coriander and Siberian fir showed a toxic effect against the brown marmorated stink bug; the death of adults on the tenth day was 100, 95.0 and 93.7 %, respectively. Also, on the soybean crops of the Arleta variety, field tests were carried out to study the efficacy of bio-rational preparations Biostop, P and an experimental preparation developed at the Federal State Budgetary Scientific Institution "Federal Research Center of Biological Plant Protection" (FSBSI FRCBPP). The experimental preparation of FRCBPP caused the death of 64.2 % of individuals of H. halys, Biostop, P – 70.1 %.

scholarly journals Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

2021 ◽  
Vol 12 ◽  
Author(s):  
Zehua Wang ◽  
Fan Yang ◽  
Ang Sun ◽  
Shuang Shan ◽  
Yongjun Zhang ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Functional Characterization ◽  
Insect Species ◽  
Pcr Analysis ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Stink Bugs ◽  
Binding Assays ◽  
Chemosensory Protein ◽  
Reverse Transcription Pcr

Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys, a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs (HhalCSP15) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone (Ki=11.9±0.6μM), cis-3-hexen-1-yl benzoate (Ki=10.5±0.4μM), and methyl (2E,4E,6Z)-decatrienoate (EEZ-MDT; Ki=9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ-MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys, and extend the olfactory function of CSPs in stink bugs.

scholarly journals Live Traps for Adult Brown Marmorated Stink Bugs

Insects ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 376 ◽  
Author(s):  
David Maxwell Suckling ◽  
Mary Claire Levy ◽  
Gerardo Roselli ◽  
Valerio Mazzoni ◽  
Claudio Ioriatti ◽  
...  
Keyword(s):  
Aggregation Pheromone ◽  
Low Cost ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
The Novel ◽  
Stink Bugs ◽  
Average Improvement ◽  
Local Materials ◽  
Wind Vane ◽  
Better Than

Surveillance for detection of the brown marmorated stink bug, Halyomorpha halys, is reliant on sticky panels with aggregation pheromone, which are low cost, but very inefficient (est. 3%). Trapping for adults was conducted in Italy with novel live (or lethal) traps consisting of aggregation pheromone-baited cylinders with a wind vane, with the upwind end covered by mesh and the downwind end sealed by a removable entry-only mesh cone, admitting the attracted bugs. The novel traps caught up to 15-times more adult H. halys than identically-baited sticky panels in two weeks of daily checking (n = 6 replicates) (the new live traps were, in Run 1, 5-, 9-, 15-, 13-, 4-, 12-, 2-fold; and in Run 2, 7-, 1-, 3-, 7-, 6-, 6-, and 5-fold better than sticky traps, daily). The maximum catch of the new traps was 96 live adults in one trap in 24 h and the average improvement was ~7-fold compared with sticky panels. The rotating live traps, which exploit a mesh funnel facing the plume downwind that proved useful for collecting adults, could also be used to kill bugs. We expect that commercially-available traps could replace the crude prototypes we constructed quickly from local materials, at low cost, as long as the principles of a suitable plume structure were observed, as we discuss. The traps could be useful for the sterile insect technique, supporting rearing colonies, or to kill bugs.

Impact of Temperature Storage Conditions of Halyomorpha halys (Hemiptera: Pentatomidae) Eggs on Parasitism by Anastatus reduvii (Hymenoptera: Eupelmidae)

2019 ◽  
Author(s):  
Emily C Ogburn ◽  
James F Walgenbach
Keyword(s):  
Storage Conditions ◽  
Halyomorpha Halys ◽  
Host Feeding ◽  
Stink Bug ◽  
Eastern United States ◽  
Stink Bugs ◽  
Different Temperatures ◽  
Anastatus Reduvii ◽  
The Impact ◽  
And Storage

Abstract Brown marmorated stink bug, Halyomorpha halys Stål, is an invasive species of Asian origin that is an important agricultural pest in the eastern United States. Sentinel egg masses are tools used to assess the impact of natural enemies on H. halys populations. To determine the effect of host egg age and storage conditions on their susceptibility to parasitism, H. halys eggs were stored at different temperatures for different lengths of time and then exposed to Anastatus reduvii (Howard), a native natural enemy of H. halys occurring in eastern North America. For eggs stored at 15, 20, and 25°C and then exposed to A. reduvii, the number of host eggs from which parasitoid offspring emerged declined with age of eggs. Control eggs (exposed to parasitoids without being stored) and those eggs stored for only 5.5 degree-days (DD) (=0.5 days) at 25°C yielded the highest percentage of parasitoids at 88.2 and 88.3%, respectively. For eggs stored at 20 and 25°C for 7.3 DD to about 36 DD, offspring emerged from about 58 to 73% of eggs, and total parasitism (emerged + unemerged parasitoids) ranged from about 70 to 80%. Parasitoid emergence was significantly lower for host eggs stored at 15°C for comparable times at 20 and 25°C. Stink bugs nymphs hatched from <0.6% of all eggs. Parasitoid-induced host egg abortion was an important component of egg mortality caused by A. reduvii, with underdeveloped stink bug nymphs, undifferentiated cell contents, and parasitoid host feeding occurring across all storage treatments.

scholarly journals Brown Marmorated Stink Bug, Halyomorpha halys (Stål) (Insecta: Hemiptera: Pentatomidae)

EDIS ◽  
2019 ◽  
Vol 2019 (1) ◽  
Author(s):  
Amanda Hodges ◽  
Cory Penca
Keyword(s):  
Life Cycle ◽  
Economic Importance ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Stink Bugs

Contents:  Introduction - Synonymy - Distribution - Description - Life Cycle - Economic Importance - Hosts - Management - Brown Marmorated Stink Bugs in Florida - Selected References This revision is also published on the Featured Creatures website at http://entnemdept.ufl.edu/creatures/veg/bean/brown_marmorated_stink_bug.htm

scholarly journals The brown marmorated stink bug Halyomorpha halys (Hemiptera: Heteroptera): A new threat for hazelnut crops in Serbia

Biljni lekar ◽  
2020 ◽  
Vol 48 (5) ◽  
pp. 488-502
Author(s):  
Aleksandar Ivezić
Keyword(s):  
Integrated Pest Management ◽  
Pest Management ◽  
Plant Protection ◽  
Management Strategies ◽  
Farming Systems ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Larval Stages ◽  
Pest Monitoring

Hazelnut crops are damaged by various types of polyphagous true bugs. The most commonly encountered species are representatives of the families Coreidae and Pentatomidae (Hemiptera: Heteroptera) Recent invasion of the exotic brown marmorated stink bug Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) represents a serious threat in many agroecosystems in Europe. Following its first detection, H. halys has become a key pest in many hazelnut crops in Europe, causing damage throughout the entire period of nut and kernel development. The pest causes damage in adults and larval stages by sucking sap from practically all parts of plants. This bug is characterized by a distinct polyphagous behavior, so to date, more than 300 plant species have been identified as food hosts. Halyomorpha halys was first registered in Serbia in October 2015 in the region of Vršac. Detection of brown marmorated bug in hazelnut crops in Serbia calls for caution and requires systematic monitoring of this pest in order to determine an adequate strategy for plant protection and accurate timing of its control. In hazelnut crops, this bug causes damage by sucking juices from the husk, shell and kernel of the fruit, which significantly reduces the quality and market value of the hazelnut fruit. Management of the brown marmorated stink bug in agricultural settings has primarily relied on the use of broad-spectrum of insecticides. In the system of Integrated Pest Management, the control of H. halys is based on a strategy that implies a reduced and effective use of insecticides and a distinct knowledge of the biology and behavior of the pest. Monitoring insect populations is a fundamental component of Integrated Pest Management programs. In a perspective of reduction or avoidance of chemical treatments and preservation of a healthy agroecosystems, such as the, alternative pest management strategies in hazelnut orchards should be further developed. Moreover, as a long-term solution, biological control of H. halys with the natural enemy species, native or introduced, could play a major role in managing this pest, especially in organic farming systems.

scholarly journals Trapping Brown Marmorated Stink Bugs: “The Nazgȗl” Lure and Kill Nets

Insects ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 433
Author(s):  
David Maxwell Suckling ◽  
Valerio Mazzoni ◽  
Gerardo Roselli ◽  
Mary Claire Levy ◽  
Claudio Ioriatti ◽  
...  
Keyword(s):  
Aggregation Pheromone ◽  
Low Cost ◽  
Detection Sensitivity ◽  
Halyomorpha Halys ◽  
Pesticide Use ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Stink Bugs ◽  
Population Suppression ◽  
Improve Detection Sensitivity

Improvements to current brown marmorated stink bug (BMSB), Halyomorpha halys, surveillance and killing systems are needed to improve detection sensitivity and to reduce pesticide use. Detection of BMSB in New Zealand with traps is reliant on sticky panels with aggregation pheromone, which are low cost but inefficient compared with beating foliage. Trapping for BMSB adults and nymphs was conducted daily with lethal traps consisting of an aggregation pheromone-baited-coat hanger covered with dark-colored long-lasting insecticide-treated mesh, we termed “The Nazgȗl”, based on its sinister appearance. A deep tray lined with white plastic was attached centrally at the base for collecting the dead BMSB. The lethal traps killed and caught up to 3.5-fold more nymphs and adult BMSB than identically-baited sticky panels in the 3 weeks of deployment, and provided a snapshot of phenology by instar. We expect that lure-and-kill stations could contribute to the suppression of a delimited population and could be included as part of a semiochemical-based eradication program. Attracting and killing females and nymphs, thus removing future offspring, could contribute to population suppression during an eradication.

scholarly journals Olfactory Responses of Trissolcus Mitsukurii to Plants Attacked by Target and Non-target Stink Bugs Suggest Low Risk for Biological Control

2021 ◽  
Author(s):  
Gabriele Rondoni ◽  
Elena Chierici ◽  
Lucrezia Giovannini ◽  
Giuseppino Sabbatini-Peverieri ◽  
Pio Federico Roversi ◽  
...  
Keyword(s):  
Host Location ◽  
Egg Parasitoid ◽  
Halyomorpha Halys ◽  
Insect Herbivores ◽  
Stink Bug ◽  
Stink Bugs ◽  
Successful Management ◽  
Olfactory Responses ◽  
Egg Deposition ◽  
Definition Of

Abstract In crop systems, successful management of invasive insect herbivores can be achieved through the introduction of exotic biocontrol agents, i.e. parasitoids or predators, having a coevolutionary history with the pest. To avert threats to local biodiversity, recent legislations require a risk assessment for the organism to be released. Evaluation of its ability to exploit, for host location, odours associated with target and non-target species is crucial for a better definition of its ecological host range. Using Y-tube olfactometer in quarantine laboratory, we investigated the ability of the Asiatic egg parasitoid Trissolcus mitsukurii (Hymenoptera: Scelionidae) to exploit odours associated with the global invader Halyomorpha halys (Hemiptera: Pentatomidae) and with non-target stink bugs native to Southern Europe. We demonstrated that T. mitsukurii is attracted by plants exposed to feeding and egg deposition of the coevolved H. halys or the native Nezara viridula, while it is not attracted by physogastric females or eggs alone. Remarkably, T. mitsukurii is repelled by plants bearing eggs of the beneficial stink bug, Arma custos. Our results usefully contribute to a more thorough and nuanced assessment of the potential non-target risks in case of massive parasitoid release.

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