scholarly journals Different soil potassium fractions and their contribution towards its uptake under long-term rice-rice cropping system in an Inceptisol of subtropical eastern India

2021 ◽  
Vol 58 (2) ◽  
pp. 262-271
Author(s):  
Raghabananda Nayak ◽  
Kumbhakarna Rout ◽  
Mitali Mandal ◽  
Abhiram Dash

A pot culture experiment was conducted by taking soils from a long term fertilizer experimental field to evaluate the contribution of different K fractions towards rice biomass yield and potassium uptake in different long term fertilizer management practices. Twelve different soils were taken for pot culture and graded dose of potassium @ 0, 20, 40, 60, 80 and 100 kg K2O ha-1 was applied. Rice variety Lalat was grown as test crop. The forms of potassium were determined in the surface soil of 0-15 cm after maturity of crop. The result shows that water soluble form of K is the major contributor to biomass yield in soils taken from100% PK, 100% NPKZn, 100% NPKZnB,100% NPKZnS,100% NPK lime and control treated plots of the main field where as exchangeable K is dominant form of K in 100% NPK and 150% NPK. Ammonium acetate K contributes to yield in NPKFYM lime treatment. The nitric acid extractable K is a major contributor to yield in 100% NPKFYM,100% N or100% NP treatment. The water soluble form of K is the major source towards uptake in 150% NPK, NPKFYM lime ,100% NPKZnB, or control. Ammonium acetate K becomes the main source by applying 100% PK,100% NPK,100% NPKZn,100% NPKZnSor 100% NPKlime whereas exchangeable K becomes major contributor by100%N,or 100% NP application and 1 N HNO3 extractable K is the main source of K uptake in NPKFYM treatment. Therefore due importance of different form of potassium should be given in soil test value for better fertilizer management.

2008 ◽  
Vol 14 (2) ◽  
pp. 181 ◽  
Author(s):  
H. SOINNE ◽  
T. PELTOVUORI

The potential effects of freezing on phosphorus (P) chemistry in Finnish soils are not well known. We studied the effects of multiple freeze-thaw cycles on soil P chemistry in a laboratory incubation experiment with one organic and one mineral surface soil. The soils were incubated at +5°C or at alternating +5/–20°C temperatures for 24 weeks, either without amendment or amended with pig slurry or with commercial compound fertilizer (NPK 20–3–9). After incubation, the soils were analyzed for water-soluble reactive and unreactive P, and acid ammonium acetate soluble P (PAAA). Freezing and thawing of soils during the incubation had no significant effect on any of the water-soluble P fractions or PAAA. The outcome was most likely a consequence of the good P status of the soils, which masked the gentle effects of freezing. According to these results, the time of soil sampling (fall vs. spring) has no effect on P test results on soils with a good P status. Concentrations of soluble P after incubation were roughly twice as high in the slurry treatments than in the fertilizer treatments, demonstrating potentially better long-term availability of slurry P.;


2017 ◽  
Vol 11 ◽  
Author(s):  
Rashid Mehmood Butt ◽  
Mohammad Saleem Akhtar ◽  
Ayaz Mehmood ◽  
Muhammad Imran ◽  
Shah Rukh ◽  
...  

Understanding of soil potassium (K) dynamics is essential for sustainable crop production. Bioavailability of potassium depends on forms and distribution within the soil profile. The objectives of this research were to determine which soil potassium forms controls the maize (<em>Zea mays</em>) potassium contents and compare the extracting capability of sodium tetraphenylborate (NaTPB) with ammonium acetate (NH<sub>4</sub>OAc) method. Nine soils representing three different parent materials <em>i.e.</em> loess, sandstone and shale were sampled at three surface genetic horizons. Within each parent material, three soils at varying level of development were selected. Besides basic soil parameters, K was fractioned into water soluble K, exchangeable K, non-exchangeable K, and NaTPB-extracted K. The maize was sown in pots having 2 kg soil from each genetic horizon. Crop was harvested at seven weeks and plant was analyzed for K contents. Results shows that NaTPB extracted K gave best correlation as compared to NH<sub>4</sub>OAc method. This conveys that a non-exchangeable K portion that becomes available to plants can better estimated by NaTPB method than NH<sub>4</sub>OAc extraction.


Response of Potassium (K) is boosted due to the introduction of high-yielding varieties and cropping intensity. So, a pot experiment was done to see the allocation of native and added K in soils and the response of this on rice. Seven soil samples from seven AEZs. An amount of 1-kilogram soil was taken in each of 28 earthen pots of which 14 pots for cultivation rice and 14 pots for without cultivation as a control. Potassium and other fertilizers applied as follows Fertilizer Recommendation Guide-1012. Two replicate plots were used per treatment. Then the soils were saturated with water so that the soils could be soaked well. The pots were kept in this condition for one day to allow the soils to settle in the pots properly. The rice variety used for the experiment was BRRI dhan28. All forms of K were found to remarkable decrease due to cultivation. The depleted non-exchangeable K sum was the largest, this was followed by exchangeable and then, in control plots, water-soluble K. The depleted amount of exchangeable and non-exchangeable K was almost similar but higher than water-soluble K in treated pots. A significant variation was found among the yields ranging from 3.77 to 5.48 g pot-1. The average dry matter yield of treated pots 4.75 g pot-1 was significantly higher than non-treated pots 3.10 g pot-1.


Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 503
Author(s):  
Györgyi Horváth ◽  
Eszter Csikós ◽  
Eichertné Violetta Andres ◽  
Tímea Bencsik ◽  
Anikó Takátsy ◽  
...  

Melilotus officinalis is known to contain several types of secondary metabolites. In contrast, the carotenoid composition of this medicinal plant has not been investigated, although it may also contribute to the biological activities of the drug, such as anti-inflammatory effects. Therefore, this study focuses on the isolation and identification of carotenoids from Meliloti herba and on the effect of isolated (all-E)-lutein 5,6-epoxide on primary sensory neurons and macrophages involved in nociception, as well as neurogenic and non-neurogenic inflammatory processes. The composition of the plant extracts was analyzed by high performance liquid chromatography (HPLC). The main carotenoid was isolated by column liquid chromatography (CLC) and identified by MS and NMR. The effect of water-soluble lutein 5,6-epoxide-RAMEB (randomly methylated-β-cyclodextrin) was investigated on Ca2+-influx in rat primary sensory neurons induced by the activation of the transient receptor potential ankyrin 1 receptor agonist to mustard-oil and on endotoxin-induced IL-1β release from isolated mouse peritoneal macrophages. (all-E)-Lutein 5,6-epoxide significantly decreased the percent of responsive primary sensory neurons compared to the vehicle-treated stimulated control. Furthermore, endotoxin-evoked IL-1β release from macrophages was significantly decreased by 100 µM lutein 5,6-epoxide compared to the vehicle-treated control. The water-soluble form of lutein 5,6-epoxide-RAMEB decreases the activation of primary sensory neurons and macrophages, which opens perspectives for its analgesic and anti-inflammatory applications.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 355
Author(s):  
Runbo Luo ◽  
Yangdong Zhang ◽  
Fengen Wang ◽  
Kaizhen Liu ◽  
Guoxin Huang ◽  
...  

The objective was to study the effects of sugar cane molasses addition on the fermentation quality and tastes of alfalfa silage. Fresh alfalfa was ensiled with no additive (Control), 1% molasses (M1), 2% molasses (M2), and 3% molasses (M3) for 206 days. The chemical composition and fermentation characteristics of the alfalfa silages were determined, the microbial communities were described by 16S rRNA sequencing, and the tastes were evaluated using an electronic tongue sensing system. With the amount of added molasses (M), most nutrition (dry matter and crude protein) was preserved and water-soluble carbohydrates (WSC) were sufficiently used to promote the fermentation, resulting in a pH reduction from 5.16 to 4.48. The lactic acid (LA) content and LA/acetic acid (AA) significantly increased, indicating that the fermentation had turned to homofermentation. After ensiling, Enterococcus and Lactobacillus were the dominant genus in all treatments and the undesirable microbes were inhibited, resulting in lower propionic acid (PA), butyric acid (BA), and NH3-N production. In addition, bitterness, astringency, and sourness reflected tastes of alfalfa silage, while umami and sourness changed with the amount of added molasses. Therefore, molasses additive had improved the fermentation quality and tastes of alfalfa silage, and the M3 group obtained the ideal pH value (below 4.5) and the best condition for long-term preservation.


Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2237
Author(s):  
Leonid Kaluzhskiy ◽  
Pavel Ershov ◽  
Evgeniy Yablokov ◽  
Tatsiana Shkel ◽  
Irina Grabovec ◽  
...  

Widespread pathologies such as atherosclerosis, metabolic syndrome and cancer are associated with dysregulation of sterol biosynthesis and metabolism. Cholesterol modulates the signaling pathways of neoplastic transformation and tumor progression. Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) catalyzes one of the key steps in cholesterol biosynthesis. The fairly low somatic mutation frequency of CYP51A1, its druggability, as well as the possibility of interfering with cholesterol metabolism in cancer cells collectively suggest the clinical importance of CYP51A1. Here, we show that the natural flavonoid, luteolin 7,3′-disulfate, inhibits CYP51A1 activity. We also screened baicalein and luteolin, known to have antitumor activities and low toxicity, for their ability to interact with CYP51A1. The Kd values were estimated using both a surface plasmon resonance optical biosensor and spectral titration assays. Unexpectedly, in the enzymatic activity assays, only the water-soluble form of luteolin—luteolin 7,3′-disulfate—showed the ability to potently inhibit CYP51A1. Based on molecular docking, luteolin 7,3′-disulfate binding suggests blocking of the substrate access channel. However, an alternative site on the proximal surface where the redox partner binds cannot be excluded. Overall, flavonoids have the potential to inhibit the activity of human CYP51A1 and should be further explored for their cholesterol-lowering and anti-cancer activity.


Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3702
Author(s):  
Anna Siemiątkowska ◽  
Maciej Bryl ◽  
Katarzyna Kosicka-Noworzyń ◽  
Jakub Tvrdoň ◽  
Iwona Gołda-Gocka ◽  
...  

Prognosis of advanced non-small cell lung carcinoma (NSCLC) is poor. Even though it can improve with anti-PD-1/PD-L1 agents, most patients do not respond to treatment. We hypothesized that the serum soluble form of the unit α of the interleukin-2 receptor (sCD25) could be used as a biomarker of successful immunotherapy in NSCLC. We recruited patients dosed with atezolizumab (n = 42) or pembrolizumab (n = 20) and collected samples at baseline and during the treatment. Levels of sCD25 were quantified with the ELISA kits. Patients with a high sCD25 at baseline (sCD25.0 ≥ 5.99 ng/mL) or/and at the end of the fourth treatment cycle (sCD25.4 ≥ 7.73 ng/mL) progressed faster and lived shorter without the disease progression and serious toxicity. None of the patients with high sCD25 at both time points continued therapy longer than 9.3 months, while almost 40% of patients with low sCD25 were treated for ≥12.3 months. There was a 6.3-times higher incidence of treatment failure (HR = 6.33, 95% CI: 2.10–19.06, p = 0.001) and a 6.5-times higher incidence of progression (HR = 6.50, 95% CI: 2.04–20.73, p = 0.002) in patients with high compared with low sCD25.0 and sCD25.4. Serum levels of sCD25 may serve as a non-invasive biomarker of long-term benefits from the anti-PD-1/PD-L1s in NSCLC.


2014 ◽  
Vol 89 ◽  
pp. 268-274 ◽  
Author(s):  
Pujun Jin ◽  
Weiqiang Zhang ◽  
Quanjun Wang ◽  
Xiaogang Yang ◽  
Sheng Sun ◽  
...  

Some of the constituent amino-acids of fibroin (degummed silk) are determined. Special attention is directed to histidine, owing to its use in the calculation of the molecular weight of fibroin. A value of 0⋅45% has been found by methods in which the histidine is isolated as nitranilate or di-(3:4-dichlorobenzenesulphonate). Other values obtained are serine 12⋅6%, threonine 1⋅5%, tyrosine 10⋅6%, and proline 1⋅5%. Hydroxyproline appears to be absent, but the presence of small amounts of some hydroxyamino-acid other than serine and threonine is indicated. The mean residue weight of fibroin is determined by three methods, one of which is a new method based on analysis of the complex formed between fibroin and cupri-ethylenediamine. This method gives a Cu:fibroin-N ratio of 1:1⋅92 and, if allowance is made for co-ordination with the tyrosine hydroxy1 group, an equivalence of 1⋅964 atoms of peptide-nitrogen to 1 atom of copper is obtained. The three methods give an average value of 78⋅0 for the mean residue weight of fibroin. This value, together with the most acceptable data for amino-acid constituents, indicate that the unidentified anhydro-residues (about 20%) have a mean residue weight of about 107. Evidence is presented that fibroin contains no amide-nitrogen. Methods for the determination of amide-nitrogen at present in use, which indicate a content of 1 to 2%, are considered to be unreliable. Fibroin dissolved in cupri-ethylenediamine gives, on neutralization and dialysis of the resulting solution, a water-soluble protein. The production of this water-soluble protein is attended by little or no degradation of the original fibroin as shown by determinations of fluidity, amino-nitrogen, and acid- and alkali-combining power. The water-soluble protein is precipitated by the normal protein-precipitating reagents, but in every instance examined the precipitated material exhibits an insolubility comparable with that of the original fibroin. Factors responsible for the solubilization process are investigated and data for molecular weight, titration values, ultra-violet absorption spectra, reducing activity, optical rotation, tryptic hydrolysis, and viscosity for both soluble and dispersed fibroin are given. Soluble fibroin has [ α ] D 15 — 53⋅1° and dispersed fibroin [ α ] D 15 — 58⋅9°, both in aqueous media. The preparation and properties of films and filaments of fibroin are described. Films of fibroin can be prepared that are water-soluble. On stretching, these films show strain-birefringence, acquire considerable tensile strength, and become insoluble in water, but X-ray examination gives the β -keratin pattern for both the stretched and unstretched films. Reasons are advanced for considering the water-soluble form of fibroin to be the native or renatured protein and the original protein to be the denatured form. The denaturation of fibroin is discussed on the basis that denaturation is essentially an unfolding of a coiled long-chain molecule. The subsequent aggregation of the uncoiled molecules to give an insoluble product is considered to be a secondary process. Some aspects of protein and polypeptide chains as macro-molecules are also discussed.


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