Gambaran histologik usus halus pada hewan coba selama 24 jam postmortem

Abstract: Studies about histological changes in small intestine are still very limited. This study was aimed to obtain the histological changes of the small intestine in several time intervals during 24 hours postmortem. This was a descriptive observational study using domestic pig as model. Samples were obtained from the ileum section of the small intestine, taken at 0 hour, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 12 hours, 14 hours, 16 hours, 18 hours, 20 hours, 22 hours, and 24 hours postmortem. The results showed that the earliest histological change was identified at 0 hours postmortem as congestion of the intestinal glands and formation of subepithelial cell spaces, followed by destruction of basal membranes of the glands at 3 hours postmortem, lysis of the glands at 16 hours postmortem. At 18-24 hours postmortem, almorst all intestinal glands could not be identified. Conclusion: The earliest histological change of small intestine was identified at 0 hours postmortem as congestion of the intestinal glands, followed by necrosis of the glands at 3 hours postmortem, and lysis of the glands at 16 hours postmortem.Keywords: small intestine, autolysis, histological changes, postmortem Abstrak: Studi mengenai perubahan gambaran histologik usus halus postmortem masih sangat terbatas. Penelitian ini bertujuan untuk mendapatkan gambaran histologik usus halus berdasarkan variasi waktu selama 24 jam postmortem. Jenis penelitian ialah deskriptif observasional dengan menggunakan babi domestik sebagai hewan coba. Sampel diambil dari bagian ileum usus halus pada interval waktu: 0 jam, 1 jam, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 7 jam, 8 jam, 9 jam, 12 jam, 14 jam, 16 jam, 18 jam, 20 jam, 22 jam, dan 24 jam postmortem. Hasil penelitian ini memperlihatkan bahwa perubahan histologik usus halus babi mulai teridentifikasi pada 0 jam postmortem berupa kongesti kelenjar intestinal, diikuti oleh kerusakan struktur membran basalis kelenjar pada 3 jam postmortem, dan lisis sel-sel kelenjar pada 16 jam postmortem. Pada 18-24 jam postmortem, hampir seluruh kelenjar intestinal tidak dapat diidentifikasi. Simpulan: Perubahan histologik awal dari usus halus dapat diidentifikasi pada 0 jam postmortem berupa kongesti kelenjar intestinal, diikuti oleh kerusakan struktur membran basalis 3 jam postmortem, dan lisis kelenjar pada 16 jam postmortem. Kata kunci: usus halus, autolisis, postmortem, waktupostmortem, perubahan histologik postmortem

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Gambaran Histopatologik Paru pada Hewan Coba Postmortem

Jurnal e-Biomedik ◽

10.35790/ebm.6.1.2018.18714 ◽

2018 ◽

Vol 6 (1) ◽

Author(s):

Sekar N. Ali ◽

Sunny Wangko ◽

Sonny J.R. Kalangi

Keyword(s):

Animal Model ◽

Smooth Muscle ◽

Observational Study ◽

Histological Change ◽

Postmortem Changes ◽

Time Of Death ◽

Histopathological Changes ◽

Alveolar Cells ◽

Time Intervals ◽

Histological Changes

Abstract: Postmortem changes play an important role in estimation of the time of death. This study was aimed to obtain the histopathological changes of lungs at several time intervals postmortem. This was a descriptive observational study using a local pig as the animal model killed by stabbing in the heart. Lung samples were taken at 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 12 hours, and 24 hours postmortem. The results showed that the earliest histological change could be identified at 60 minutes postmortem in the form of alveolar dilatation. At 2 hours postmortem, congestion of smooth muscle layers of bronchioles was observed. The epithelial cells of the alveoli were undetected at 3 hours postmortem meanwhile the smooth muscle layers were undetected at 12 hours postmortem. At 24 hours postmortem, the bronchioles were still detected but the structure of their layers could not be identified. Conclusion: Histopathological changes were observed as alveolar dilatation at 60 minutes postmortem, followed by congestion of muscle layers, and undetected epithelial alveolar cells as well as smooth muscle layers. At 24 hours postmortem, bronchioles were still detected but the structure of their layers could not be identified.Keywords: histological changes, lungs, postmorterm Abstrak: Perubahan postmortem berperan penting untuk memperkirakan waktu kematian. Penelitian ini bertujuan untuk mendapatkan perubahan histopatologik paru hewan coba postmortem pada beberapa interval waktu. Jenis penelitian ialah deskriptif-observasional menggunakan hewan coba satu ekor babi lokal yang dimatikan dengan tikaman pada jantung. Sampel paru diambil dalam waktu 15 menit, 30 menit, 45 menit, 60 menit, 75 menit, 90 menit, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 12 jam dan 24 jam postmortem. Hasil penelitian mendapatkan pada 60 menit postmortem terjadi dilatasi alveoli. Pada 2 jam postmortem tampak kongesti lapisan otot polos. Pada 3 jam postmortem sel-sel epitel alveoli tidak tampak lagi dan pada 12 jam postmortem lapisan otot polos tidak terdeteksi. Pada 24 jam postmortem bronkiolus masih dapat dideteksi tetapi struktur lapisannya tidak teridentifikasi. Simpulan: Perubahan awal histopatologik paru babi postmotem ini dimulai pada 60 menit postmortem berupa dilatasi alveoli, diikuti kongesti lapisan otot polos, serta tidak terdeteksinya sel-sel epitel alveoli dan lapisan otot polos. Pada 24 jam postmortem bronkiolus masih terdeteksi tetapi struktur lapisannya tidak teridentifikasi lagi.Kata kunci: perubahan histologik, paru, postmortem

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Gambaran histologik otot jantung pada hewan coba postmortem

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.13731 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Tonci Ubruangge ◽

Sunny Wangko ◽

Sonny J.R. Kalangi

Keyword(s):

Observational Study ◽

Heart Muscle ◽

Time Of Death ◽

Occipital Area ◽

Modus Operandi ◽

Time Intervals ◽

Normal Appearance ◽

Domestic Pig ◽

Histological Changes ◽

Unnatural Death

Abstract: Commonly, unnatural death is complicated and causes difficulties for to the investigating officers to reveal the modus operandi and the time of death. Estimation of postmortem interval is very important in the investigation, albeit, it is frequently put the investigators to a debate. This study aimed to obtain the histological changes of heart muscle at several time intervals postmortem. This was a descriptive observational study. A domestic pig weighed ±20kg was used as model. The pig was killled by conducting a blunt trauma at its occipital area. Heart muscle samples were taken at several time intervals as follows: 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 105 minutes, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 12 hours, and 24 hours postmortem. The histological changes were observed with an Olympus CX21 microscope and Optilab. The percentages of those changes were evaluated at 5 high-power fields (400x) for each sample. The results showed that less than 30% of myocytes had flattened and denser nuclei at 105 minutes postmortem and were associated with hydrophic degeneration at 2 hours postmortem. Those changes became widely spreaded and at 24 hours postmortem they could be identified in 60-80% of myocytes. Albeit, the striated pattern could still be identified until 24 hours postmortem. Conclusion: The histological changes of heart muscle postmortem namely morpholocical changes of the nuclei and hydrophic degeneration could be identified at 2 hours postmortem. At 24 hours postmortem those changes were distributed widely among the myocytes, however, the striated pattern could still be identified. Moreover, there were still focal areas with normal appearance. Keywords: histological changes, cardiac muscle, postmortem Abstrak: Kematian tidak wajar yang dialami seseorang dengan berbagai motif kejahatan dapat menyulitkan para penyidik dalam menggungkapkan modus operandi dan waktu kematian. Penentuan lama kematian k orban sangat dibutuhkan dan sering menjadi perdebatan di peradilan. Penelitian ini bertujuan untuk mendapatkan perubahan histologik jaringan otot jantung hewan coba pada beberapa interval waktu postmortem. Jenis penelitian ialah deskriptif-observasional menggunakan hewan coba satu ekor babi lokal dengan berat badan ±20kg. Hewan coba dimatikan dengan pukulan benda tumpul pada area osipital. Sampel otot jantung diambil pada beberapa interval waktu: 15 menit, 30 menit, 45 menit, 60 menit, 75 menit, 90 menit, 105 menit, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 12 jam, dan 24 jam postmortem. Perubahan histologik diamati dengan mikroskop Olympus CX21 dan Optilab. Persentase perubahan tersebut dievaluasi pada 5 lapang pandang pembesaran 400x. Hasil penelitian mendapatkan <30% miosit dengan inti yang memipih dan memadat pada 105 menit postmortem serta degenerasi hidropik 2 jam postmortem. Perubahan-perubahan tersebut makin meluas dan pada 24 jam postmortem telah tampak pada 60-80% miosit jantung tetapi corak seran lintang tetap dapat diidentifikasi. Simpulan: Perubahan histologik berupa perubahan morfologik inti disertai degenerasi hidropik mulai tampak pada 2 jam postmortem dan pada 24 jam postmortem perubahan tersebut telah terdistribusi luas. Walaupun demikian, pada 24 jam postmortem corak seran lintang masih dapat diidentifikasi. Juga terdapat area-area fokal dengan jaringan otot jantung yang masih tampak normal.Kata kunci: perubahan histologik, otot jantung, postmortem

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Perubahan Histologik Postmortem pada Kelenjar Brunner Hewan Coba

Jurnal e-Biomedik ◽

10.35790/ebm.6.1.2018.19526 ◽

2018 ◽

Vol 6 (1) ◽

Author(s):

Reza S. Sartika ◽

Djon Wongkar ◽

Sonny J.R. Kalangi

Keyword(s):

Digestive System ◽

Gland Cell ◽

Cell Lysis ◽

Descriptive Study ◽

Time Intervals ◽

Domestic Pig ◽

Histological Changes ◽

Gland Cells ◽

Brunner Glands

Abstract: Studies of postmortem histological changes in digestive system showed a variety of changes related to postmortem time. This study was aimed to obtain the histological changes of Brunner gland within 24-hour interval using a domestic pig as model. This was a descriptive study. Samples of duodenum were taken at several time intervals, as follows: 0 hour, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 12 hours, 14 hours, 16 hours, 18 hours, 20 hours, 22 hours, and 24 hours. The results showed that the earliest histological changes occurred at 3 hours postmortem which showed congestion, widened gland lumen, as well as hydrophic degeneration of gland cells. At 7 hours to 22 hours postmortem the changes became more visible and were associated with lysis of the gland cells. At 24 hours postmortem the lysis had covered >50% of the gland cells, however, the Brunner glands could still be identified. Conclusion: The earliest changes occured at 3 hours postmortem in the forms of congestion, widened gland lumens, and hydrophic degeneration of gland cells which became more visible at 7-22 hour postmortem associated with gland cell lysis. The cell lysis covered most of the gland cells at 24 hours postmortem, however, the Brunner glands could still be identified.Keywords: duodenum, autolysis, postmortem. Abstrak: Berbagai penelitian mengenai perubahan histologik postmortem dari organ sistem pencernaan telah dilakukan pada hewan coba yang memperlihatkan variasi perubahan histologik sehubungan dengan waktu postmortem. Penelitian ini bertujuan untuk mendapatkan gambaran perubahan histologik postmortem pada kelenjar Brunner hewan coba dengan interval waktu 24 jam. Jenis penelitian ialah deskriptif yang menggunakan babi domestik sebagai hewan coba. Interval waktu pengambilan sampel dari bagian duodenum ialah: 0 jam, 1 jam, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 7 jam, 8 jam, 9 jam, 10 jam, 12 jam, 14 jam, 16 jam, 18 jam, 20 jam, 22 jam, dan 24 jam postmortem. Hasil penelitian mendapatkan bahwa perubahan awal terjadi pada 3 jam postmortem yang menunjukkan kongesti dan lumen kelenjar melebar, serta degenerasi hidropik pada sel-sel kelenjar Brunner. Perubahan semakin tampak pada 7 jam sampai 22 jam postmortem, dan mulai terjadi lisis sel-sel kelenjar Brunner. Pada 24 jam postmortem lisis yang terjadi telah mencapai sekitar 50% sel kelenjar tetapi kelenjar Brunner masih dapat diidentifikasi. Simpulan: Perubahan awal terjadi pada 3 jam postmortem yang menunjukkan kongesti dan lumen kelenjar melebar serta degenerasi hidropik pada sel-sel kelenjar Brunner yang terus berlangsung disertai lisis sel-sel kelenjar yang pada 24 jam postmortem telah mencapai sebagian besar sel kelenjar tetapi kelenjar Brunner masih dapat diidentifikasi.Kata kunci: duodenum, autolisis, postmortem

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Gambaran histologik gaster pada hewan coba selama 24 jam postmortem

Jurnal e-Biomedik ◽

10.35790/ebm.4.1.2016.12146 ◽

2016 ◽

Vol 4 (1) ◽

Author(s):

Megi Lilingan ◽

Sonny J.R. Kalangi ◽

Sunny Wangko

Keyword(s):

Gland Cell ◽

Histological Change ◽

Descriptive Study ◽

Postmortem Interval ◽

Fundic Gland ◽

Time Intervals ◽

Histological Changes ◽

Gland Cells ◽

Almost All

Abstract: Studies about postmortem histological changes in stomach is still very limited. This study aimed to obtain histological changes of stomach in several time intervals during 24 hours postmortem. This is a descriptive study using pig as model. Samples of fundus tissue taken in several time intervals in postmortem were as follows: 0 hour, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 12 hours, 14 hours, 16 hours, 18 hours, 20 hours, 22 hours, and 24 hours. The results showed that the earliest histological change was identified at 2 hours postmortem as fundic gland cell congestion. At 7 hours postmortem the contours of some fundic glands and borders of their cells were not distinct anymore, meanwhile their nuclei were dispersed among the remnants of fundic glands. At 18-24 hours postmortem, almost all fundic glands could not be indetified. Conclusion: The earliest histological change of stomach was identified at 2 hours postmortem as fundic gland cell congestion, followed by necrosis of fundic gland cells since 7 hours postmortem.Keywords: postmortem histological changes, fundic glands, postmortem interval Abstrak: Studi mengenai perubahan gambaran histologik gaster postmortem masih sangat terbatas. Penelitian ini bertujuan untuk mendapatkan gambaran histologik gaster berdasarkan variasi waktu selama 24 jam postmortem. Jenis penelitian ini deskriptif dengan menggunakan babi sebagai hewan coba. Sampel jaringan fundus diambil pada interval waktu 0 jam; 1 jam; 2 jam; 3 jam; 4 jam; 5 jam; 6 jam; 7 jam; 8 jam; 9 jam; 12 jam; 14 jam; 16 jam; 18 jam; 20 jam; 22 jam; 24 jam postmortem. Hasil penelitian memperlihatkan bahwa perubahan histologik gaster babi mulai teridentifikasi pada 2 jam postmortem sebagai kongesti kelenjar fundus. Pada 7 jam postmortem bentuk dari beberapa kelenjar fundus dan batas-batas sel tidak jelas, sementara itu inti sel mulai terpisah di antara sisa-sisa kelenjar fundus. Pada 18-24 jam postmortem, umumnya kelenjar fundus sudah tidak bisa diindentifikasi. Simpulan: Perubahan histologik awal dari gaster dapat diidentifikasi pada 2 jam postmortem dengan gambaran kongesti kelenjar fundus, diikuti oleh nekrosis kelenjar fundus sejak 7 jam postmortem. Kata kunci: perubahan histologik postmortem, kelenjar fundus, waktu postmortem.

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GAMBARAN HISTOLOGIK GINJAL HEWAN COBA POSTMORTEM

Jurnal e-Biomedik ◽

10.35790/ebm.2.2.2014.4555 ◽

2014 ◽

Vol 2 (2) ◽

Author(s):

Baldatun Rahmadana ◽

Sunny Wangko ◽

Sonny J. R. Kalangi

Keyword(s):

Histological Change ◽

Postmortem Interval ◽

Proximal Tubules ◽

Time Intervals ◽

Histological Changes ◽

Medicolegal Investigation ◽

Tubular Lumen ◽

Proximal Tubuli ◽

The Right ◽

Early Postmortem

Abstract: Estimation of postmortem interval plays some significant roles in medicolegal investigation. This was a descriptive experimental study using one local pig as model. Samples were taken from the right and left kidneys at several time intervals: 0 minute, 15 minutes, 30 minutes, 45 minutes, 60 minutes, 12 hours, and 24 hours postmortem. The results showed several histological changes, as follows: hydrophic degeneration in a small part of proximal tubules 30 minutes postmortem that increased after 45 minutes associated with narrowing of Bowman cavities; necrosis of glomeruli and proximal tubules associated with irregular distal tubular lumen and widening of Bowman cavities 60 minutes postmortem; necrosis of distal tubuli 12 hours postmortem; and necrosis of most kidney structures 24 hours postmortem. Conclusion: Hydrophic degeneration of proximal tubuli is the earliest histological change 30 minutes postmortem, followed by necrosis of glomeruli as well as proximal and distal tubuli that worsened after 24 hours postmortem. It is expected that this study can provide valuable contribution to medicolegal investigation, especially in early postmortem interval estimation.Keywords: postmortem interval, histological changes, postmortem, kidneyAbstrak: Penentuan saat kematian sangat penting dalam penyelidikan medikolegal. Penelitian ini merupakan penelitian deskriptif-eksperimental dengan menggunakan satu ekor babi lokal sebagai obyek penelitian. Sampel diambil dari ginjal kanan dan kiri pada beberapa interval waktu; 0 menit; 15 menit; 30 menit; 45 menit; 60 menit; 12 jam; dan 24 jam postmortem. Hasil penelitian memperlihatkan degenerasi hidropik pada sebagian kecil tubuli proksimal 30 menit postmortem yang meluas setelah 45 menit disertai penyempitan kavum Bowman; pelebaran kavum Bowman, nekrosis glomeruli dan tubuli proksimal, lumen sebagian kecil tubuli distal ireguler 60 menit postmortem; nekrosis tubuli distal 12 jam postmortem; dan nekrosis hampir seluruh struktur-struktur tersebut 24 jam postmortem. Simpulan: Degenerasi hidropik tubuli proksimal merupakan perubahan histologik yang paling dini yaitu 30 menit postmortem, disusul oleh tanda-tanda nekrosis pada sebagian kecil glomeruli dan tubuli proksimal serta nekrosis tubuli distal yang meluas setelah 24 jam postmortem. Penelitian ini diharapkan dapat memberikan kontribusi yang bermakna untuk kepentingan medikolegal, terutama dalam perkiraan saat kematian dini.Kata kunci: saat kematian, perubahan histologik, ginjal

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HISTOLOGICAL CHANGES IN THE SMALL INTESTINE IN THE ADVANCED STAGES OF BURN DISEASE

World of Medicine and Biology ◽

10.26724/2079-8334-2017-3-61-90-96 ◽

2017 ◽

Vol 13 (61) ◽

pp. 090

Author(s):

G. M. Galunko

Keyword(s):

Small Intestine ◽

Histological Changes ◽

Advanced Stages ◽

Burn Disease

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mRNA expression profiles of P450 3A enzymes in the liver and small intestine of the domestic pig

Research in Veterinary Science ◽

10.1016/j.rvsc.2011.06.013 ◽

2012 ◽

Vol 93 (1) ◽

pp. 360-365 ◽

Cited By ~ 9

Author(s):

Min Yao ◽

Menghong Dai ◽

Zhaoying Liu ◽

Wenlong Cui ◽

Daoyuan Li ◽

...

Keyword(s):

Small Intestine ◽

Mrna Expression ◽

Expression Profiles ◽

Domestic Pig

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Adenocarcinomas of the Intestine Induced in Syrian Hamsters by N-methyl-N-nitrosourea

Pathologia veterinaria ◽

10.1177/030098586900600504 ◽

1969 ◽

Vol 6 (5) ◽

pp. 403-412

Author(s):

Katherine McD. Herrold

Keyword(s):

Small Intestine ◽

Cytological Change ◽

Histological Changes ◽

Syrian Hamsters ◽

Histological Types ◽

Absorptive Cells ◽

Cytological Features

The adenocarcinomas of the intestine induced in Syrian hamsters by N-methyl-N-nitrosourea (NMU) were of two histological types, superficial and intestinal. These types had distinctive characteristics regarding pattern, cytological features, secretion of mucus, and mode of growth. The histological changes induced by NMU in the mucosa of the small intestine differed from what has been described in enzootic intestinal adenocarcinoma and proliferative ileitis of Syrian hamsters. NMU produced alteration in the villous architecture and cytological change in the absorptive cells. There was marked shortening of the villi and reduced thickness of the mucosa. The villous absorptive cells were large and cuboidal with centrally placed nuclei.

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MORPHOLOGICAL AND HISTOLOGICAL EFFECTS OF BRUCEINE A ON THE LARVAE OF AEDES AEGYPTI LINNAEUS (DIPTERA: CULICIDAE)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i10.27315 ◽

2018 ◽

Vol 11 (10) ◽

pp. 422

Author(s):

Dwi Sutiningsih ◽

Mustofa Mustofa ◽

Tri Baskoro Tunggul Satoto ◽

Edhi Martono

Keyword(s):

Epithelial Cells ◽

Aedes Aegypti ◽

Histological Change ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Histological Changes ◽

Brucea Javanica ◽

Gastrointestinal Damage

Objective: This study aimed to determine a target of action of bruceine A on the basis of its morphological and histological effects on the larvae of Aedes aegypti Linnaeus.Methods: Bruceine A was isolated from Brucea javanica (L.) Merr. seeds in accordance with the Mangungsong method. Larvae of A. Aegypti (L.) in instar III to the beginning of instar IV were treated with various concentrations of bruceine A. The negative control group did not receive any treatment, whereas the positive control group received 1 ppm temefos. Dead larvae were collected after 24 h of treatment for the examination of morphological and histological changes.Results: The negative control group did not exhibit any morphological and histological changes. Larvae treated with bruceine A, however, had visible damaged heads, cuticles, digestive and respiration tracts, respiratory siphons, and setae, and they were smaller than normal larvae. Larvae treated with temefos exhibited gastrointestinal damage, narrowed breathing tubes, cuticle damage, and detached/damaged seta feathers. The necrosis of gastrointestinal epithelial cells was the major histological change exhibited by larvae treated with various concentrations of bruceine A or 1 ppm temefos.Conclusion: The targets of action of bruceine A in A. aegypti (L.) larvae are the head/caput, cuticle, setae, siphon, and gastrointestinal and respiratory tracts.

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