scholarly journals POLA KUMAN AEROB PENYEBAB DIARE PADA ANAK BALITA DAN UJI KEPEKAAN ANTIBIOTIK DI BAGIAN ILMU KESEHATAN ANAK RSUP PROF. DR. R.D. KANDOU MANADO PERIODE MARET 2011-SEPTEMBER 2011

2013 ◽  
Vol 4 (3) ◽  
Author(s):  
Velma Buntuan ◽  
Sarah M Warouw
Keyword(s):  
Escherichia Coli ◽  
General Hospital ◽  
Enterobacter Aerogenes ◽  
Klebsiella Pneumonia ◽  
Klebsiella Aerogenes ◽  
Soft Or ◽  
Under Five ◽  
Sensitivity Tests ◽  
Causative Agents ◽  
Defecation Frequency

Abstract: Diarrhoea is an increase of defecation frequency greater than usual (more than three times daily), associated with changes of the form and consistency of stools, soft or fluidy, and with or without mucus or blood. The causative agents of diarrhoea are e.g. Campylobacter, Salmonella, Shigella, dan Escherichia coli. Microbes’ sensitivities against antibiotics can change from time to time, and from area to area. The purpose of this study was to find out the profile of aerobes that caused diarrhoea in childen under five admitted to the Children’s Department, at the Prof Dr.R.D. Kandou General Hospital, as well as the sensitivity and resistency tests of microbes against antibiotics. Feces samples of the children were cultured, and microbial identifications and sensitivity tests against antibiotics were done. The causative microbes were Escherichia coli (40%); and Klebsiella aerogenes, Klebsiella pneumonia, and Enterobacter aerogenes (each of them 3.3%). Sensitivity tests against meropenem were 96.7% and against amikacin 90.0%. Resistency tests to antibiotics showed sulpha methoxazole-trimethoprim 100%, cefazolin 100%, chloramphenicol 96.7%, doxycicline 83.3%, ampicillin 100%, and ceftriaxon 60%. Conclusion: the microbe that most caused diarrhoea in children under five admitted to the Children’s Department of the Prof dr.R.D. Kandou General Hospital was Escherichia coli, and the most sensitive antibiotics against this microbe were meropenem and amikacin.Key words: microbes’ profile, diarrhoea, under five, sensitivity, antibioticsAbstrak: Diare adalah frekuensi buang air besar yang bertambah dari biasanya (lebih dari tiga kali), ditandai dengan perubahan bentuk dan konsistensi tinja yang lembek sampai cair, dengan atau tanpa darah atau lendir dalam tinja. Penyebab diare antara lain: Campylobacter, Salmonella, Shigella, dan Escherichia coli. Kepekaan kuman terhadap antibiotik dapat berbeda dari waktu ke waktu dan dari daerah ke daerah. Tujuan penelitian ini untuk mendapatkan pola kuman aerob penyebab diare pada balita, serta kepekaan dan resistensi kuman terhadap antibiotik. Feses balita yang rawat inap di Bagian Ilmu Kesehatan Anak RSUP Prof. Dr. R.D Kandou Manado dengan diare dibuat kultur, identifikasi kuman, dan uji kepekaan terhadap antibiotik. Kuman penyebab diare yaitu Escherichia coli (40%), Klebsiella aerogenes, Klebsiella pneumonia, serta Enterobacter aerogenes (masing-masing 3,3%). Kepekaan kuman terhadap antibiotik meropenem 96,7% dan amikacin 90,0%. Resistensi antibiotik diperoleh terhadap sulpha methoxazole trimethoprim sebesar 100%, cefazolin 100%, chloramphenicol 96,7%, doxycicline 83,3%, ampicillin 100%, dan ceftriaxon 60%. Simpulan: kuman penyebab diare terbanyak pada anak balita yang dirawat inap di Bagian Anak RSUP Prof Dr. R.D. Kandou Manado ialah Escherichia coli dan kepekaan antibiotik yang terbaik ialah meropenem dan amikasin.Kata kunci: pola kuman, diare, balita, kepekaan, antibiotik

scholarly journals Pola Kuman Penyebab Ventilator Associated Pneumonia(VAP) dan Sensitivitas Terhadap Antibiotik di RSAB Harapan Kita

Sari Pediatri ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 384
Author(s):  
Retno Widyaningsih ◽  
Latre Buntaran
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Clinical Laboratory ◽  
Enterobacter Aerogenes ◽  
National Committee ◽  
Klebsiella Pneumonia ◽  
Ventilator Associated Pneumonia ◽  
Pseudomonas Sp ◽  
Bacillus Sp

Latar belakang. Peningkatan resistensi antibiotik di antara bakteri penyebab pneumonia nosokomial yang didapat di rumah sakit telah banyak dilaporkan. Jika kita tidak mengenal pola kepekaan kuman di suatu rumah sakit akan menyulitkan pemberian terapi empiris awal. Tujuan. Mengetahui profil kuman penyebab pneumonia yang didapat di rumah sakit pada anak serta uji sensitivitas terhadap beberapa antibiotik.Metode. Studi deskriptif retrospektif dengan sumber data yang berasal dari rekam medis Laboratorium Mikrobiologi RSAB Harapan Kita periode Januari hingga Juni 2010. Spesimen adalah semua spesimen saluran respiratorik dari pasien dengan diagnosis pneumonia yang dirawat. Biakan dan uji resistensi dilakukan menurut standar National Committee for Clinical Laboratory Standards(NCCLS).Hasil. Didapatkan 116 spesimen biakan dan di antaranya 4 (3,4%) steril. Dari 112 biakan positif, 79.5% di antaranya adalah bakteri gram negatif berturut-turut dari yang paling dominan adalah Pseudomonas sp.(22,4%), Pseudomonas aeruginosa(18,1%), Stenotrophomonas maltophilia(9.5%), Serratia marcescens(8,6%),Enterobacter aerogenes(7,8%), Klebsiella pneumonia, Bacillus sp., dan Escherichia coli(masing-masing 5,2%). Golongan Pseudomonasmemiliki sensitivitas terhadap ceftazidime, amikacin serta netilmicin.Kesimpulan. Basil gram negatif aerob (79,5%) merupakan mikroorganisme penyebab yang paling dominan. Ceftazidime, diikuti terhadap amikacin serta netilmicin masih mempunyai sensitivitas yang tinggi sehingga dapat dipakai sebagai terapi awal VAP.

scholarly journals Microbial Assessment of Bed Linens in Ekiti State University Students’ Hostels

2020 ◽  
pp. 43-52
Author(s):  
T. O. Olowomofe ◽  
J. O. Oluyege ◽  
A. O. Ogunlade ◽  
D. T. Makinde
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Proteus Mirabilis ◽  
Disease Transmission ◽  
Enterobacter Aerogenes ◽  
Antibiotics Resistance ◽  
Klebsiella Aerogenes ◽  
State University ◽  
Plasmid Profiling

Bed linen is clearly recognized as a potential reservoir for microorganisms and could be a vector of disease transmission. The present study was aimed at isolating and characterizing bacteria and fungi from different kinds of bed linen of student in Ekiti State University hostels. Pour plate method was used for the enumeration of total bacteria count from the posterior and anterior end of the bed linen. The average bacteria count for the anterior and posterior end was 7.46 and 7.16 Log10 CFU/ml respectively. The most dominant microbial species were bacteria and these were mostly found in the environment and on human skin. The bacteria isolated were Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis, Enterobacter aerogenes, Bacillus cereus, Escherichia coli and Klebsiella aerogenes. Bacillus cereus had the highest frequency of occurrence (25%), followed by Staphylococcus aureus (15%), Escherichia coli (15%), Klebsiella aerogenes (15%), Staphylococcus epidermis (15%), Enterobacter aerogenes (10%) and Proteus mirabilis (5%). The fungi isolates were Aspergillus sulphureus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Fusarium spp. Antibiotics susceptibility test was carried out on the bacteria isolates with gram negative bacteria showing resistance to Cotrimoxazole and gram positive bacteria showing resistance to Amplicillin. Most of the bacteria isolates have multiple antibiotics resistance. The bacterial isolates were susceptible to Ciprofloxacin. Plasmid profiling was also done with Escherichia coli having three high molecular weight plasmids, Bacillus cereus and Enterobacter aerogenes do not possess plasmid. The identified species are suspected to be opportunistic pathogens for human, representing a risk for people with weakened immune system, especially in cases of super-infection.

scholarly journals ISOLATION AND IDENTIFICATION OF MICROBIAL FAUNA IN UTI PATIENTS

2022 ◽  
Vol 71 (6) ◽  
pp. 2242-44
Author(s):  
Muhammad Ilyas ◽  
Saffora Riaz ◽  
Ali Hassan
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Enterobacter Aerogenes ◽  
Klebsiella Pneumonia ◽  
Bacterial Isolates ◽  
Proteus Vulgaris ◽  
Isolation And Identification ◽  
Males And Females

Urinary tract infection caused by bacteria leads to inflammation. It is an important infection which can cause a highly morbidity rates in both males and females. The aim of the present study was determine the percentage prevalence of the UTI among the general population and the percentage of the bacterial isolates in the Urine samples. Among 150 samples bacterial isolates were obtained which included Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa. The highest prevalence of specific bacteria was found to be Escherichia coli recorded among all the UTI patients to be (55.55%) the least percentage among the bacteria were Klebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa and Enterobacter aerogenes which recorded 16.66%, 5.55%, 11.11% and 11.11% respectively. Results of the study showed that of femaleshad UTI more than the males.

scholarly journals Clonal Propagation and Antibacterial Activity of Moringa Peregrina (Forssk) Fiori Plant

2016 ◽  
Vol 6 (1) ◽  
pp. 787-797 ◽  
Author(s):  
Linda Naim Hasan Alrayes ◽  
Wesam “Moh’d Hadi” Al Khateeb ◽  
Mohamad Awad Mohamad Shatnaw
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Moringa Peregrina

Moringa peregrina (Forssk) Fiori is one of the known medicinal plants in Jordan. It is used in traditional medicine to treat rheumatism and infections. Plant parts are used in the indigenous systems of human medicine for the treatment of a variety of ailments. Therefore, this study was conducted to investigate factors affecting M. peregrina in vitro propagation and its antimicrobial activity. Microshoots with apical meristem (10 or 15 mm in length) were subculture on MS medium supplemented with different concentration of BAP, Kinetin, Zeatin and Thidiazuron (TDZ). Maximum number of new shoots/explant (4.39) was obtained on Murashige and Skoog agar medium supplemented with 1.6 mg/L Zeatin. While at 0.4 mg/L BAP maximum shoot length was obtained (37.78 mm). Antibacterial activity of aqueous, methanolic, ethanolic, and acetonic extracts of both in vitro plantlets and ex vitro (field grown) M. peregrina were evaluated by the agar welldiffusion method against Klebsiella oxytoca ATCC 18182, Salmonella typhimurium ATCC 19430, Methicillin resistant, Staphylococcus aureus ATCC 29974, Klebsiella pneumonia ATCC13883, Proteus vulgaris ATCC 13315, Proteus mirabilis ATCC 12453, Enterobacter aerogenes ATCC 35029, Pseudomonas aeruginosa ATCC 27253, Escherichia coli O157:H7, Staphylococcus aureus ATCC 25923, Salmonella paratyphi ATCC 13076 and Escherichia coli ATCC 29522. The obtained results showed that ethanolic, acetonic and aqueous extracts revealed a wide antibacterial activity. Ethanolic extract of in vitro M. peregrina plantlets showed the maximum inhibition zone against Staphylococcus aureus.  

Антимікробна резистентність провідних збудників інфекцій сечових шляхів у Києві (Україна)

2017 ◽  
Vol 1 (2) ◽  
pp. 48-60
Author(s):  
A.G. Salmanov ◽  
A.V. Rudenko
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Providencia Rettgeri ◽  
E Coli

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.

scholarly journals A Novel Antimicrobial Phenanthrene Alkaloid from Bryopyllum pinnatum

2011 ◽  
Vol 8 (3) ◽  
pp. 1456-1461 ◽  
Author(s):  
Donatus Ebere Okwu ◽  
Fred Uchenna Nnamdi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Klebsiella Pneumonia ◽  
Antimicrobial Studies ◽  
Prevention And Treatment ◽  
Kalanchoe Pinnata ◽  
Isolated Compound

Phenenthrene alkaloid identified as 1-ethanamino 7 hex-1-yne-5I-one phenanthrene was isolated from the ethanolic extract of the leaves ofBryophyllum pinnatum(syn. B. calcinum kalanchoe pinnata) a versatile Nigeria medicinal plant. The structure was elucidated using NMR, IR, UV and MS spectral data. Antimicrobial studies showed that the isolated compound successfully inhibitedPsuedomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Escherichia coli, Candida albicansandAspergillus niger. This result authenticates the use ofbryophyllum pinantumin phytomedicine for disease prevention and treatment of infections.

scholarly journals Characterization of Anti-Bacterial Effect of the Two New Phages against Uropathogenic Escherichia coli

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1348
Author(s):  
Lívia Slobodníková ◽  
Barbora Markusková ◽  
Michal Kajsík ◽  
Michal Andrezál ◽  
Marek Straka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Therapeutic Potential ◽  
Medical Intervention ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Phage Cocktail ◽  
Causative Agents ◽  
Tract Infections

Urinary tract infections (UTIs) are among the events that most frequently need medical intervention. Uropathogenic Escherichia coli are frequently their causative agents and the infections are sometimes complicated by the presence of polyresistant nosocomial strains. Phage therapy is a tool that has good prospects for the treatment of these infections. In the present study, we isolated and characterized two bacteriophages with broad host specificity against a panel of local uropathogenic E. coli strains and combined them into a phage cocktail. According to genome sequencing, these phages were closely related and belonged to the Tequatrovirus genus. The newly isolated phages showed very good activity on a panel of local clinical E. coli strains from urinary tract infections. In the form of a two-phage cocktail, they were active on E. coli strains belonging to phylogroups B2 and D, with relatively lower activity in B1 and no response in phylogroup A. Our study is a preliminary step toward the establishment of a national phage bank containing local, well-characterized phages with therapeutic potential for patients in Slovakia.

Fate of Escherichia coli O157:H7 and Other Coliforms in Commercial Mayonnaise and Refrigerated Salad Dressing

1995 ◽  
Vol 58 (1) ◽  
pp. 13-18 ◽  
Author(s):  
ERROL V. RAGHUBEER ◽  
JIM S. KE ◽  
MICHAEL L. CAMPBELL ◽  
RICHARD S. MEYER
Keyword(s):  
Escherichia Coli ◽  
Storage Temperature ◽  
Enterobacter Aerogenes ◽  
Antimicrobial Effect ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Salad Dressing ◽  
E Coli ◽  
Survival Pattern ◽  
High Level

Commercial mayonnaise and refrigerated ranch salad dressing were inoculated at two levels with two strains of Escherichia coli O157:H7, a non-pathogenic E. coli, and the non-fecal coliform Enterobacter aerogenes. Results showed that at the high inoculation level (>106 colony forming units [CFU]/g) in mayonnaise stored at room temperature (ca. 22°C) both strains of O157:H7 were undetected at 96 h. At the high inoculation level, all strains of coliform bacteria tested survived longer in salad dressing stored at 4°C than in mayonnaise stored at 22°C. The O157:H7 strains were still present at low levels after 17 days. The survival time in the low-level inoculum (104CFU/g) study decreased, but the survival pattern in the two products was similar to that observed in the high-level inoculum study. Slight differences in survival among strains were observed. The greater antimicrobial effect of mayonnaise may be attributable to differences in pH, water activity (aw), nutrients, storage temperature, and the presence of lysozyme in the whole eggs used in the production of commercial mayonnaise. Coliform bacteria survived longer in refrigerated salad dressing than in mayonnaise particularly at the high-level inoculum. Both mayonnaise (pH 3.91) and salad dressing (pH 4.51) did not support the growth of any of the microorganisms even though survival was observed.

Sign in / Sign up

Export Citation Format

Share Document