scholarly journals Antibacterial Activity Testing on APMS (p-Methoxy Cinnamic Acid) Against Escherichia coli Bacteria

2021 ◽  
Vol 21 (2) ◽  
pp. 155
Author(s):  
Ahmad Fuad Masduqi ◽  
Erwin Indriyanti ◽  
Rahmawati Salsa Dinurrosifa
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Cinnamic Acid ◽  
Knoevenagel Condensation ◽  
Condensation Reaction ◽  
Inhibition Zone ◽  
Sonochemical Method ◽  
Knoevenagel Condensation Reaction ◽  
Ft Ir ◽  
Escherichia Coli Bacteria

This study aims to determine whether the compound APMS (p-methoxy cinnamic acid) has the antibacterial activity of Escherichia coli and to determine the effective concentration of the compound APMS (p-methoxy cinnamic acid) in inhibiting the growth of Escherichia coli bacteria. This research begins with the synthesis of APMS obtained through the knoevenagel condensation reaction with the sonochemical method. The synthesized compounds were tested organoleptically and their melting points were measured. The structure of the results was elucidated using FT-IR and GC-MS, then tested the antibacterial activity of APMS compounds against Escherichia coli. Data analysis with SPSS, 99% confidence level (p<0.01) and continued with ANOVA test. The compound synthesized by APMS is in the form of fine crystals with a glossy white color, has a characteristic odor, and produces a % yield of 92.71%. The level of p-methoxy cinnamic acid (APMS) produced from this study was 95% using the GC-MS Instrument. APMS compounds have antibacterial activity against Escherichia coli. The average inhibition zone formed at a concentration of 5%; 10%; and 15% respectively are 0.695 cm; 0.727 cm; and 0.855 cm The optimal concentration of this study was at a concentration of 15% which gave the greatest inhibitionKeywords: Antibacterial; Escherichia coli; p-Methoxy cinnamic acidUji Aktivitas Antibakteri Senyawa APMS (Asam p_Metoksi Sinamat) Terhadap Bakteri Escherichia coli  ABSTRAKPenelitian ini bertujuan untuk mengetahui apakah senyawa APMS (Asam p-metoksisinamat) memiliki aktivitas antibakteri Escherichia coli dan mengetahui konsentrasi efektif dari senyawa APMS (Asam p-metoksisinamat) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini diawali dengan sintesis APMS ini didapatkan melalui reaksi kondensasi knoevenagel dengan metode sonokimia. Senyawa hasil sintesis diuji organoleptis dan diukur titik leburnya. Struktur hasil dielusidasi menggunakan  FT-IR dan GC-MS, kemudian uji aktivitas antibakteri senyawa APMS terhadap Escherichia coli. Analisis data dengan SPSS, tingkat kepercayaan 99% (p<0,01) dan dilanjutkan uji ANOVA. Senyawa hasil sintesis APMS berupa kristal halus berwarna putih mengkilap, memiliki bau khas, dan menghasilkan % yield sebesar 92,71%. Kadar Asam Para Metoksisinamat (APMS) yang dihasilkan dari penelitian ini sebesar 95% menggunakan Instrumen GC-MS. Senyawa APMS memiliki aktivitas antibakteri terhadap Escherichia coli. Rataan zona hambat yang terbentuk pada konsentrasi 5%; 10%; dan 15% secara berturut adalah 0,695 cm; 0,727 cm; dan 0,855 cm Konsentrasi yang optimal dari penelitian ini adalah pada konsentrasi 15% yang memberikan daya hambat yang paling besar.Kata kunci:  Antibakteri; Asam p-metoksi sinamat; Escherichia coli

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals Microwave Assisted Synthesis of Indole Substituted Alkenes Using Knoevenagel Condensation Reaction and their Antibacterial Activity Study

2010 ◽  
Vol 7 (s1) ◽  
pp. S543-S551 ◽  
Author(s):  
Shubha Jain ◽  
B. Nagi Reddy ◽  
K. Sambasiva Rao ◽  
G. Neeliah
Keyword(s):  
Antibacterial Activity ◽  
Reaction Rate ◽  
Knoevenagel Condensation ◽  
Condensation Reaction ◽  
Microwave Oven ◽  
Microwave Assisted Synthesis ◽  
Microwave Assisted ◽  
Knoevenagel Condensation Reaction ◽  
New Compounds ◽  
Active Methylene

The reaction rate of Knoevenagel condensation can be dramatically enhanced by irradiating the reaction mixture containing an aldehyde, active methylene compound,L-proline and chlorobenzene with a commercial microwave oven. Twenty Knoevenagel condensation products were synthesized within 5-17 min in good yields. The new compounds were also screened for their antibacterial activity.

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals Synthesis and Antibacterial Testing of Imina Derivative Compounds from Piperonal and Anilin

2018 ◽  
Vol 21 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Dimas Syamsudin ◽  
Ismiyarto Ismiyarto ◽  
Ngadiwiyana Ngadiwiyana
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Melting Point ◽  
1H Nmr ◽  
Antibacterial Agent ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
Inhibition Zone

Synthesis of Imine derivates used as antibacterial activity has been done. Synthesis was conducted by condensation reaction between piperonal and aniline. The product synthesis was characterized by melting point, LC-MS, FTIR and 1H-NMR, then application of the product as antibacterial towards bacteria Staphylococcus aureus and Escherichia coli. The product of synthesis was pale-brown solid, and had melting point at 56-61 0C. Identification by LC-MS showed the compound molecular weight was 225.30 gram/mol, identification by FTIR showed a compound contained imine C=N at 1620.21 cm-1, C-H aromatic at 1581.63 cm-1, Csp3-H at 2962.37 cm-1 from CH2, and C-O at 1265.30 cm-1, Identification by 1H-NMR showed a compound had a singlet peak at δ=6 ppm that proton was from the CH2, multiplet peak at δ=6.9-7.7 ppm proton from two aromatics and singlet peak at δ=8.4 ppm from proton imine. The minimum inhibitory concentration of the product towards Staphylococcus aureus and Escherichia coli was 1 mg/mL with diameter inhibition zone 2 mm and 3 mm. From these data it could be concluded that the compound 3,4- methlenedioxybenzilyimine was formed and it had ability as an antibacterial agent.

scholarly journals Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 201 ◽  
Author(s):  
Suryadi Budi Utomo ◽  
Mita Fujiyanti ◽  
Warih Puji Lestari ◽  
Sri Mulyani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Hexadecyltrimethylammonium Bromide ◽  
E Coli ◽  
Escherichia Coli Bacteria

<p>This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the <em>S.</em><em> </em><em>aureus</em> bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against <em>E. coli</em>.</p>

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ALGA Caulerpa racemosa DARI PERAIRAN PULAU MANTEHAGE MINAHASA UTARA TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 905
Author(s):  
Yosefa E.H. Ritan ◽  
Defny S. Wewengkang ◽  
Jainer Pasca Siampa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Chloroform Fraction ◽  
Caulerpa Racemosa ◽  
Gram Positive Bacteria ◽  
Chemical Content ◽  
Escherichia Coli Bacteria

ABSTRACTAlgae Caulerpa racemosa are able to produce chemical content that leave the potential to be antibacterial. The purpose of this study was to determine whether the extracts and fractions of algae Caulerpa racemosa had antibacterial activity against the Gram-negative bacteria Escherichia coli and the Gram-positive bacteria Staphylococcus aureus. The sample was extracted using the maceration method with 95% ethanol and the fractionation method used was liquid-liquid fractionation. Antibacterial activity testing was carried out using the disc diffusion agar method. The results obtained from the antibacterial activity test on Escherichia coli bacteria produce an inhibition zone in all extracts and fractions used while Staphylococcus aureus bacteria produced an inhibition zone for all  extracts and fractions, in ethanol extract with inhibitory value 6.72mm, n-hexane fraction 7.53mm, chloroform fraction 6.93mm and fraction methanol 7.32mm, respectively. The extracts and fractions of algae Caulerpa racemosa samples showed no activity against Escherichia coli, while Staphylococcus aureus has performed antibacterial activity with moderate category. The conclusion of this study, extracts and fractions of algae caulerpa racemosa had no antibacterial activity on the bacteria Escherichia coli while bacteria Staphylococcus aureus had antibacterial activity with moderate inhibition.Keywords: Algae Caulerpa racemosa, Antibacterial, Escherichia coli, Staphylococcus aureusABSTRAKAlga Caulerpa racemosa mampu menghasilkan kandungan kimia yang berpotensi sebagai antibakteri. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri  ekstrak dan fraksi dari alga Caulerpa racemosa di Perairan Pulau Mantehage Minahasa Utara terhadap bakteri Escherichia coli sebagai bakteri Gram-negatif dan Staphylococcus aureus sebagai bakteri Gram-positif. Sampel diekstraksi dengan menggunakan metode maserasi dengan pelarut etanol 95% dan metode fraksinasi yang digunakan yaitu fraksinasi cair-cair. Pengujian aktivitas antibakteri yang dilakukan menggunakan metode difusi agar. Hasil yang didapat dari uji aktivitas antibakteri pada bakteri Escherichia coli tidak menghasilkan zona hambat pada semua ekstrak dan fraksi yang digunakan sedangkan pada bakteri Staphylococcus aureus menghasilkan zona hambat pada semua ekstrak dan fraksi yaitu, ekstrak etanol dengan daya hambat 6,72mm, fraksi n-heksan 7,53mm, fraksi kloroform 6,93mm dan fraksi metanol 7,32mm. Kesimpulan dari penelitian ini, ekstrak dan fraksi dari sampel alga Caulerpa racemosa tidak memiliki aktivitas pada bakteri Escherichia coli sedangkan pada bakteri Staphylococcus aureus memiliki aktivitas antibakteri dengan daya hambat yang sedang.Kata Kunci: Alga Caulerpa racemosa, Antibakteri, Escherichia coli, staphylococcus aureus

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF LIME JUICE EXTRACT AGAINST ESCHERICHIA COLI

2020 ◽  
Vol 3 (1) ◽  
pp. 135-139
Author(s):  
Nerdy Nerdy ◽  
Palas Tarigan ◽  
Elysa Elysa ◽  
Puji Lestari ◽  
Siti Nurmalisa
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Lime Juice ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Escherichia Coli Bacteria

Lime (Citrus aurantifolia Swingle) is known to have seceral phytochemical contents (flavonoids and saponins) that can be memiliki berbagai aktivitas farmakologis yang bermanfaat. Salah satu aktivitas farmaokologis yang diberikan oleh flavonoid adalah sebagai antibacterial activity. The aims of this research was to determine the antibacterial activity of lime juice against Escherichia coli bacteria. This research used an experimental laboratory research method using antibacterial effectiveness trials against the growth of Escherichia coli bacteria. The research design for antibacterial effectiveness used the lime fruit extract method with 4 treatment groups, positive control and negative control. Antibacterial effectiveness was observed the inhibition zone diameter by disc diffusion method. Results of the Escherichia coli antibacterial effectiveness test obtained at a concentration of 100% had antibacterial activity with an average diameter of the inhibition zone 19.9 mm. The lowest concentration that still provides antibacterial resistance against Escherichia coli is at a concentration of 12.5% ​​with an average diameter of the inhibition zone of 17.9 mm. The results obtained indicated that the antibacterial effectiveness test on lime fruit extract is very strong. At a concentration of 100% it has the best antibacterial activity, the inhibition zone diameter similar with the inhibition zone of 500 mg tetracycline.

scholarly journals Seleksi Aktivitas Antibakteri dari Beberapa Gorgonia dari Perairan Manado Terhadap Bakteri Staphylococcus aureus, Escherichia coli, dan Edwarsiella tarda

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 220
Author(s):  
Febby Ester Fany Kandou ◽  
Pience Veralyn Maabuat ◽  
Deidy Yulius Katili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Inhibitory Zone ◽  
Purposive Sampling ◽  
Three Samples ◽  
Two Samples ◽  
Escherichia Coli Bacteria ◽  
Halo Zone

Telah dilakukan penelitian dengan tujuan menguji aktivitas antibakteri dari beberapa Gorgonia yang berasal dari Perairan Pulau Bunaken Manado. Tahapan penelitian yaitu pengambilan sampel gorgonia pada lokasi Perairan Bunaken Manado dengan metode purposive sampling, identifikasi, ekstraksi dengan metode maserasi, peremajaan bakteri uji dengan metode goresan, pembuatan larutan Mc. Farland, pengujian aktivitas antibakteri dengan metode Kirby-Bauer. Bakteri uji yang digunakan adalah Staphylococcus aureus, Escherichia coli, dan Edwarsiella tarda. Hasil penelitian dari ke 9 sampel Gorgonia teridentifikasi 7 sampel Gorgonia yaitu Annella sp, Melithaea sp, Astrogorgia sp, Mopsella sp *cf, Siphonogorgia geodeffry *cf,  Gorgonia sp, dan Iciligorgia sp *cf serta 2 sampel yang belum teridentifikasi. Pengujian antibakteri didapatkan untuk bakteri uji Staphylococcus aureus, ada 4 sampel yang memiliki zona hambat dengan kategori sedang, 1 sampel dengan kategori kuat dan 4 sampel tidak memiliki zona hambat tetapi memiliki zona halo. Bakteri uji Escherichia coli, ada 5 sampel memiliki zona hambat dengan kategori sedang, 1 sampel dengan kategori kuat dan 3 sampel tidak memiliki zona hambat tetapi memiliki zona halo. Bakteri uji Edwarsiella tarda, ada 2 sampel memiliki zona hambat dengan kategori sedang, dan 7 sampel tidak memiliki zona hambat tetapi memiliki zona halo. Berdasarkan hasil penelitian dapat disimpulkan bahwa ke 9 sampel Gorgonian memiliki sifat bakterisida (membunuh bakteri) dan bakteriostatik (menghambat pertumbuhan bakteri)The aim of the research to examine the antibacterial activity of some Gorgonian from Bunaken Island Waters. The stages of the research are Gorgonian sampling, identification, extraction by maceration method, making Mc. Farland solution, and testing the antibacterial activity by the Kirby-Bauer method. The test bacteria used Staphylococcus aureus, Escherichia coli, and Edwarsiella tarda. The results of the nine Gorgonian samples were identified namely Annella sp, Melithaea sp, Astrogorgia sp, Mopsella sp *cf, Siphonogorgia geodeffry *cf, Gorgonia sp, and Iciligorgia sp and three unidentified samples. Antibacterial testing was obtained for the Staphylococcus aureus bacteria, there were four samples that had medium inhibitory categories, one sample with strong category and four samples did not have inhibitory zones but had a halo zone. Escherichia coli bacteria, there are five samples having inhibition zone with medium categories, one sample with strong category and three samples do not have inhibitory zone but have a halo zone. Edwarsiella tarda bacteria, there are two samples having inhibition zone with medium categories, and seven samples do not have inhibitory zone but have a halo zone. Based on the results of the study it can be concluded that the nine Gorgonian samples have bactericidal and bacteriostatic properties

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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