scholarly journals EFFECT OF GRADUAL HIGH SALINITY ON SOME STRESS PARAMETERS (GLUCOSE,TOTAL PROTEIN AND LACTATE) IN BLOOD PLASMA OF COMMON CARP

2017 ◽  
Vol 48 (2) ◽  
Author(s):  
Al- Khshali & Al- Hilalli
Keyword(s):  
Blood Plasma ◽  
Common Carp ◽  
Total Protein ◽  
High Salinity ◽  
Tap Water ◽  
Control Treatment ◽  
Average Weight ◽  
Water Control ◽  
Stress Parameters ◽  
In Blood Plasma

The present study was conducted to investigate the effect of high salinity on the some stress parameters of common carp (Cyprinus carpio) which gradually exposed to salt concentrations of 5, 10 and 15g/liter, as well as tap water (control 0.1g/liter) for 90 days .80 fish were randomly distributed on eight glass tanks with 2 replicates as 10 fish / replicate at average weight of 15 ± 3 g to study the effect of salinity on the Total protein , Glucose ,and Lactate in blood plasma , Fish were fed during the trial on commercial diet with 31.9 % protein content.. Results showed that an increase in the total protein to 4.98 and then decrease to 4.60  and 3.90 g/100 cm3 when the salinity increased to 5, 10 and 15 g/l respectively, compared with the control treatment (4.94 g/100 cm3).  Glucose reached 75.11, 91.20 and 95.17 mg/100 ml at the concentrations of 5, 10 and 15 g/l respectively, compared with the control treatment (66.64 mg/100ml). Lactate also increased to 43.12, 45.38  and 48.53  mg/100 ml , when the salinity increased to  5 , 10 and 15 g/l respectively, in comparison with control treatment  (40.65 mg/100 ml) .

scholarly journals Effect of Different Salinity Concentrations on the Chemical analysis and Time Appearance of Feces for Cyprinus Carpio

2019 ◽  
Vol 7 (2) ◽  
pp. 28
Author(s):  
Hasan Ali Al Hilali ◽  
Mohammed Shaker Al-Khshali
Keyword(s):  
Body Composition ◽  
Chemical Analysis ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Salt Concentration ◽  
Tap Water ◽  
The Body ◽  
Control Treatment ◽  
Average Weight ◽  
Appearance Time

The present study was conducted to investigate the effect of high salinity on some stress parameters of common carp (Cyprinus carpio), which gradually exposed to salt concentrations of 5, 10 and 15g/liter, as well as tap water (water without chlorine) (control 0.1g/liter) for 90 days. Eighty fish were randomly distributed on eight glass tanks with 2 replicates, 10 fish / replicate at average weight of 15 ± 3 g, to study the effect of salinity on the time appearance of feces and, the chemical analysis of the body composition .The results showed that the appearance time of feces was decreased with the rise of salt concentrations of common carp. As the passage of food through digestive tract took less time in high salinities. and the appearance of feces was after 4.16 hours of feed intake in a salt concentration of 15 g/liter, whereas it started after 5.22 hours in a saline concentration of 10 g/l and after 6.11 in a salt concentration of 5g/l in comparison with the control treatment (6.17 hours). Regarding the chemical analysis of the body composition, it was found that the moisture dropped with the rise of salinity, as it mounted to 77.09, 74.94 and 73.80% in the salt concentrations 5,10 and 15 g/l respectively, in comparison with the control treatment (78.45%).The percentage of protein decreased to 14.19, 13.89 and 13.74% in the salt concentrations 5, 10 and 15 g/l respectively, in comparison with the control treatment (14.28%).The percentage of fat amounted to 5.71, 5.68, and 5.66% in the salt concentrations 5,10 and 15 g/l respectively, in comparison with the control treatment (5.73%). whereas the percentage of ash was increased to 3.01, 5.40 and 6.80% with the increase in salinity to 5,10 and 15 g/l respectively ,compared with the control treatment (1.80%).

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

scholarly journals Effect of prochloraz fungicide on neopterin and biopterin concentrations in blood plasma of common carp

2014 ◽  
Vol 83 (2) ◽  
pp. 101-105 ◽  
Author(s):  
Petr Maršálek ◽  
Ivana Mikuliková ◽  
Helena Modrá ◽  
Zdeňka Svobodová
Keyword(s):  
High Performance Liquid Chromatography ◽  
Blood Plasma ◽  
Common Carp ◽  
High Performance ◽  
Pesticide Exposure ◽  
Cell Mediated Immunity ◽  
Traditional Role ◽  
Reverse Phase ◽  
Exposed Fish ◽  
In Blood Plasma

Neopterin and biopterin are often used as markers of cell mediated immunity. The aim of this study was to evaluate the effect of prochloraz on neopterin and biopterin concentrations in blood plasma of common carp as possible indicators for monitoring perturbations of the immune system caused by xenobiotics like pesticides. Prochloraz is a widely used imidazole fungicide in horticulture and agriculture. A total of 60 juvenile common carp were divided into four groups of 15 fish and exposed to prochloraz at concentrations of 0, 50, 150 and 380 µg·l-1, respectively. The total length of exposure was 28 days. Neopterin and biopterin concentrations were determined by reverse phase high performance liquid chromatography with fluorescence detection. Our results indicated that concentrations of neopterin (25 ± 7.6 nmol·l-1) and biopterin (190 ± 29 nmol·l-1) in plasma of untreated common carp were comparable with those in mammals. Neopterin concentrations significantly (P< 0.01) increased after exposure to prochloraz in comparison to non-exposed fish, while biopterin concentrations were not influenced by exposure to prochloraz. This study contains new data on neopterin and biopterin concentrations in fish plasma and investigates neopterin and biopterin in their non-traditional role as markers of cell mediated immunity of fish associated with pesticide exposure.

scholarly journals The Effect of Handling and Transport of the Concentration of Glucose and Cortisol in Blood Plasma of Common Carp

1999 ◽  
Vol 68 (4) ◽  
pp. 265-274 ◽  
Author(s):  
Z. Svobodová ◽  
P. Kaláb ◽  
L. Dušek ◽  
B. Vykusová ◽  
J. Kolářová ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Common Carp ◽  
In Blood Plasma

THE PENETRATION OF IODINE-LABELLED FOLLICLE-STIMULATING HORMONE AND ALBUMIN INTO THE SEMINIFEROUS TUBULES OF SHEEP AND RATS

1972 ◽  
Vol 54 (1) ◽  
pp. 67-77 ◽  
Author(s):  
B. P. SETCHELL ◽  
A. L. C. WALLACE
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Blood Plasma ◽  
Human Serum ◽  
Total Protein ◽  
Follicle Stimulating Hormone ◽  
Volume Of Distribution ◽  
Rete Testis ◽  
Seminiferous Tubules ◽  
In Blood Plasma

SUMMARY The entry of 125I-labelled ovine follicle-stimulating hormone (FSH) and human serum albumin into the seminiferous tubules of rams has been studied by measuring radioactivity in blood plasma and rete testis fluid after an intravenous injection of the labelled protein. After the injection of FSH, radioactivity/mg total protein in rete testis fluid exceeded that in blood plasma from the first day onwards, but after the injection of human serum albumin, radioactivity/mg total protein in rete testis fluid only reached that in plasma after 4–5 days and never exceeded it. Protein-bound radioactivity disappeared from the blood more quickly after an injection of FSH than after an injection of albumin. The volume of distribution for ovine FSH in the testes of rats was greater than that for human serum albumin. The volume of distribution for FSH was greater, in absolute terms, in the testes with the efferent ducts ligated 24 h previously than in the contralateral control testes when the FSH was injected at the time of efferent duct ligation. The volume of distribution for albumin was slightly less in the ligated testes than in the control testes. This suggests that FSH but not albumin had penetrated into the fluid trapped inside the testis. The liver and kidney had much higher concentrations of protein-bound radioactivity per unit weight than did the testis after the injection of FSH, so it does not seem that FSH is selectively taken up by the testis.

scholarly journals Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods

2005 ◽  
Vol 48 (3) ◽  
pp. 385-388 ◽  
Author(s):  
Dimas Augusto Morozin Zaia ◽  
Fábio Rangel Marques ◽  
Cássia Thaïs Bussamra Vieira Zaia
Keyword(s):  
Comparative Study ◽  
Blood Plasma ◽  
Total Protein ◽  
Total Proteins ◽  
Erythrosin B ◽  
Spectrophotometric Methods ◽  
Biuret Method ◽  
Bradford Method ◽  
In Blood Plasma

A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.

scholarly journals Effects of Salinity on the Macro- and Micronutrient Contents of a Halophytic Plant Species (Portulaca oleracea L.)

Land ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 481
Author(s):  
Gulom Bekmirzaev ◽  
Baghdad Ouddane ◽  
Jose Beltrao ◽  
Mukhamadkhon Khamidov ◽  
Yoshiharu Fujii ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Species ◽  
High Salinity ◽  
Tap Water ◽  
Water Solution ◽  
Experimental Studies ◽  
Portulaca Oleracea ◽  
Control Treatment ◽  
Halophytic Plant ◽  
Salinized Soil

The main purpose of the two consecutive experimental studies presented here was to compare the effect of salinity on nutrients in leaves of the halophytic plant species Portulaca oleracea L. and in soil. The first experiment was conducted to study the effect of salinity on plant growth, biomass accumulation, yield, root layer development, salt accumulation, and the dynamics of changes in mineral substances in plants and soil. In the second experiment, P. oleracea seeds were sown directly into salinized soil (treated immediately before plant growth) to determine the nutrient levels in leaves and soil. Three salinity treatments (saline water solution with NaCl: T1, 5 dS m−1; T2, 9.8 dS m−1; and T3, 20 dS m−1) and a control treatment (T0, 1 dS m−1) were used in the first experiment. The soil in the second experiment was used in a previous study (performed immediately before P. oleracea growth) (salinized soil: T1, 7.2 dS m−1; T2, 8.8 dS m−1; T3, 15.6 dS m−1; T0, 1.9 dS m−1). The plants were irrigated with tap water at amounts in the range of 0.25–0.50 L/pot. Analysis of the experimental results showed that P. oleracea is resistant to salinity, is able to remove ions (400–500 kg ha−1 NaCl), and can be grown in saline soil. The results indicated that P. oleracea is able to grow in high-salinity soil. This finding was confirmed by the dry matter obtained under high-salinity conditions. Salinity stress affected nutrient uptake in leaves and soil.

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