scholarly journals PREPARATION OF JUNIPERUS EXTRACT AND DETECTION OF ITS ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY

2019 ◽  
Vol 50 (4) ◽  
Author(s):  
Maeh &et al.
Keyword(s):  
Prostate Cancer ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Activity ◽  
Cytotoxicity Activity ◽  
Juniperus Phoenicea ◽  
Antimicrobial And Antioxidant Activity

 This study was aimed to produce silver nanoparticles by fruits extract of Juniperus phoenicea and comparison between the ethanolic extract and nano extract through the antioxidant, antibacterial, anti-parasite and cytotoxicity against prostate cancer. The synthesis of silver nanoparticles was shown by many characterizing techniques: UV, FTIR, XRD and SEM. Nano extract exhibited a higher antibacterial, antioxidant, antiparasite compared to ethanolic extract and higher cytotoxicity activity. The nano extract exhibited higher antibacterial activity compared with antibiotics. Finally, we study the toxicity of J. phoenicea by Inhibition of RBC hemolysis by H2O2, the results exhibited the highest inhibition activity of J. phoenicea against H2O2. The nanoextract of J. phoenicea can be used effectively in the production of potential antioxidant, antiparasite antimicrobial and anticancer.

scholarly journals PHYTOCHEMICAL SCREENING AND GAS CHROMATOGRAPHY-MASS SPECTROSCOPY ANALYSIS OF BIOACTIVE COMPOUNDS AND BIOSYNTHESIS OF SILVER NANOPARTICLES USING SPROUT EXTRACTS OF VIGNA RADIATA L. AND THEIR ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY

2019 ◽  
pp. 180-184
Author(s):  
SIVAKUMAR T
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Mass Spectroscopy ◽  
Vigna Radiata ◽  
Green Gram ◽  
Hexadecanoic Acid ◽  
Gas Chromatography Mass Spectroscopy

Objectives: The present study was aimed to investigate the facile synthesis of silver nanoparticles (AgNPs) using the green gram sprout extract (GGSE) of Vigna radiata L. and also in vitro studies of antioxidant and antimicrobial activities. Methods: Gas chromatography-mass spectroscopy techniques have been used for the qualitative and quantitative evaluation of the phytochemicals present in the green gram seedlings. The antioxidant activity of AgNPs and GGSE was analyzed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. In vitro antibacterial activity was performed using the agar well diffusion method. Results: The presence of various secondary metabolites such as flavonoids, steroids, terpenoids, alkaloids, amino acids, polyphenol, glycoside, and protein was found in samples. The major chemical compounds of V. radiata were n-hexadecanoic acid, stigmasterol, caffeine, hexadecanoic acid, cholest-5-en-3-ol (3.beta.)-, and cyclopentane. The percentage of DPPH activity was enhanced on increasing the concentration of AgNPs. In vitro antibacterial effect of the diverse concentrations of AgNPs was investigated against each Gram-negative (Klebsiella aerogenes, and Escherichia coli) and Gram-positive (Bacillus substilis and Staphylococcus aureus) bacterial strains. Conclusion: The result suggests that biosynthesized AgNPs have good antibacterial and antioxidant activity and might be a potential for the bioactive components.

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals Study of preparations of bee pollen extracts, antioxidant and antibacterial activity

2007 ◽  
Vol 31 (6) ◽  
pp. 1818-1825 ◽  
Author(s):  
Solange Teresinha Carpes ◽  
Rosicler Begnini ◽  
Severino Matias de Alencar ◽  
Maria Lúcia Masson
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Activity Index ◽  
Ethanolic Extract ◽  
Ethanol Solution ◽  
Pollen Extract ◽  
Extraction Condition ◽  
Polyphenol Compounds ◽  
Significant Difference

The aim of this study was to determine the antioxidant activity, phenolic content and antibacterial activity of pollen extracts obtained with different concentrations of ethanol. Each extraction condition (ethanol solutions from 40 to 90%) had a different effect in the phenolic compounds content. Although, the pollen extract obtained at 60, 70 and 80% of ethanol showed relatively higher levels of phenolic compounds (>10 mg/g) and did not present statistical significant difference between the extraction conditions. The amount of total phenolics ranged from 3.6 to 8.1 and 6.6 to 10.9 mg GAE/g for Alagoas state and Parana state pollen, respectively. The higher value for antioxidant activity index was 83.30% for the pollen from Alagoas state and 81.15 % for Parana state pollen. The highest degree of antioxidant activity was found in the extraction at 60% of ethanol solution for Parana state pollen, which also showed the highest concentration of polyphenol compounds. Staphylococcus aureus was inhibited by the ethanolic extract of Alagoas state pollen in all the concentrations of solvent, except the ethanolic extract of pollen at 90%. The extract at 60% of ethanol solution (Parana sample) inhibited Bacillus subtilis, Pseudomonas aeruginosa and Klebsiella sp.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI DARI DERIVAT METIL EKSTRAK ETANOL DAUN GAMBIR (Uncaria gambir)

2020 ◽  
Vol 15 (4) ◽  
pp. 145
Author(s):  
IRMA KRESNAWATY ◽  
ACHMAD ZAINUDDIN
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Chemistry Laboratory ◽  
Bacteria Growth ◽  
Ft Ir ◽  
Uv Visible ◽  
Chloroform Methanol

<p>ABSTRAK</p><p>Banyak tanaman yang dilaporkan memiliki kandungan senyawabahan aktif antioksidan dan antibakteri. Salah satu tanaman Indonesia yangmemiliki aktivitas ini adalah gambir (Uncaria gambir). Pada penelitian ini,ekstrak etanol daun gambir diubah menjadi derivat metilnya untukmembuatnya lebih larut dalam lemak dan diamati pengaruh derivatisasitersebut terhadap aktivitas antioksidan di laboratorium kimia organik danpengujian aktivitas antibakteri di laboratorium mikrobiologi UniversitasPadjadjaran. Penelitian ini dilaksanakan di laboratorium Kimia OrganikJurusan Kimia dan laboratorium Mikrobiologi Jurusan Biologi UniversitasPadjadjaran dari bulan Desember 2004 - Juli 2005. Ekstrak gambirdimetilasi  menggunakan  dimetil  sulfat  (DMS)  dan  dimurnikanmenggunakan kromatografi kolom dengan pelarut bergradien (kloroform :metanol = 99:1 ; 98:2 ; 95:5 ; 80:20 ; 70:30; dan 50:50 v/v) dan kemudianmenggunakan kloroform : metanol = 99 : 1 v/v. Aktivitas antioksidanmenunjukkan penurunan yang tampak dari peningkatan, yaitu : IC 50  13,41ppm untuk ekstrak etanol menjadi 121,81 ppm untuk hasil metilasi.Aktivitas antibakteri juga menunjukkan penurunan setelah dimetilasikarena adanya penurunan diameter hambat pertumbuhan bakteri. Duaisolat (isolat 1 dan 2) yang diperoleh dari hasil pemurnian dikarakterisasimenggunakan spektrofotometer UV-Visible dan FT-IR. Hasil yangdiperoleh mengindikasikan adanya senyawa fenolik yang ditunjukkan olehregang –OH pada 3445 dan 3448 cm -1 dan regang CH aromatik pada 3010dan 3030 cm -1 . Isolat 1 memiliki aktivitas antioksidan dan antibakteri yanglebih tinggi dibandingkan isolat 2.</p><p>Kata kunci : Uncaria gambir, derivat metil, antibakteri dan aktivitasantioksidan</p><p>ABSTRACT</p><p>The antioxidant and antibacterial activities of ethanolextract of gambir leaves (Uncaria gambir)</p><p>There are many plants in Indonesia reported to contain antioxidantand antibacterial substances. One of them having these activities is gambir(Uncaria gambir). In this research, ethanol extract of gambir leaves waschanged into its methyl derivate in order to make it more soluble in fats.The effect of the derivatization on antioxidant activity was observed atorganic chemistry laboratory and antibacterial activity was observed atmicrobiology laboratory of the Padjadjaran University. This research wascarried out in December 2004 to July 2005. Gambir extract wasmethylized using dimethylsulphate (DMS) and then purified usingcoloumn chromatography with gradient solvents (chloroform : methanol =99:1; 98:2; 50:50; 80:20; 70:30; and 50:50 v/v), and then with chloroform :methanol = 99:1 v/v. The antioxidant activity showed a decrease indicatedby an increase of IC 50 from 13.41 ppm for ethanolic extract to become121.81 ppm for the methylated compounds. The antibacterial activity alsoshowed a decrease after methylization due to the decrease of inhibitiondiameter of bacteria growth. Two isolates (isolate 1 and 2) obtained fromthe  purification  steps  were  characterized  using  UV-Visiblespectrophotometer and FT-IR. The results indicated the existence ofphenolic compunds showed by -OH stretching in 3,445 and 3,448 cm -1 ;and CH aromatic stretching in 3,010 and 3,030 cm -1 . Isolate 1 was higherin antioxidant and antibacterial activities than isolate 2.</p><p>Key words : Uncaria gambir, methyl derivative, antibacterial, antioxidantactivities</p>

scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF AMOMUM SUBULATUM ROXB. OF NEPAL

2017 ◽  
Vol 10 (4) ◽  
pp. 95
Author(s):  
Bimala Subba ◽  
Tank Raj Seling ◽  
Ram Chandra Kandel ◽  
Ganga Prasad Phuyal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Drug ◽  
Traditional Use ◽  
Low Altitude ◽  
Amomum Subulatum ◽  
Antimicrobial And Antioxidant Activity ◽  
Antibacterial And Antioxidant Activity

Objective: The present study was designed to study and compare the antibacterial and antioxidant activity of methanol extracts of Amomum subulatum Roxb. seeds collected from two different regions of Nepal.Methods: Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl assay and Antibacterial activity was measured by agar well-diffusion method.Results: Results showed that both sample extracts had interesting antibacterial activity against all test microorganisms and had remarkable radical scavenging potential to be used as an antioxidant. Sample B (i.e., collected from high altitude Taplejung district) exerted comparative quit higher antimicrobial and antioxidant activity than the Sample A (i.e., is collected from low altitude Panchthar district). However, the standard antibacterial drug Tetracycline exhibits superior activity than the extracts.Conclusions: The results suggest that both samples (i.e., seed extract of A. subulatum Roxb.) possess almost similar moderate antibacterial and antioxidant activity in comparison to standards which justifies the traditional use of this plant.Keywords: Extract, Cardamom, Activity, Medicinal plants, Seeds, Bacillus pumilus.

Green synthesis of silver nanoparticles using Atrocarpus altilis leaf extract and the study of their antimicrobial and antioxidant activity

2016 ◽  
Vol 180 ◽  
pp. 264-267 ◽  
Author(s):  
Veerasamy Ravichandran ◽  
Sethu Vasanthi ◽  
Sivadasan Shalini ◽  
Syed Adnan Ali Shah ◽  
Rajak Harish
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Antimicrobial And Antioxidant Activity

scholarly journals Polymer Capped Silver Nanoparticles from Ziziphus nummularia Leaves Extract: Potent Antibacterial and Antioxidant Activity

2021 ◽  
Vol 18 (4) ◽  
pp. 691-701
Author(s):  
Faruk Arodiya ◽  
Chirag Makvana ◽  
Kokila Parmar
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Fourier Transform ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Ziziphus Nummularia ◽  
Antimicrobial And Antioxidant Activity

Generally, synthesis and encapsulation process improve therapeutic value of nano encapsulated drugs. The silver nanoparticles (AgNPs) biosynthesized from Ziziphus nummularia leaves and encapsulated with polyvinyl pyrrolidone (PVP) polymer as antibacterial agents, due to its high bioavailability, better encapsulation and less toxic properties. The nanoparticles (AgNPs) biosynthesized from Ziziphus nummularia leaves and capped with polyvinyl pyrrolidone (PVP) polymer, The acquired AgNPs and polymeric functionalized AgNPs were fully characterised by the UV- Visible spectroscopy , Transmission electron microscopy (TEM), X-Ray diffraction pattern (XRD) and Fourier transform infrared spectroscopy (FTIR).The crystalline Ag NPs and Polymer Functionalized AgNPs have a face-centered cubic structure with an average size of 9.20 nm, according to X-ray Diffraction spectroscopy. Fourier Transform Infrared spectroscopy revealed that biomolecules such as proteins are incapable of reducing metal ions and the formation of an encapsulating layer in terms of metal ions. High-Resolution transmission electron microscopy revealed that Polymer functionalized AgNPs ranged in size of 10 nm. AgNPs and Polymer functionalized AgNPs showed effective antimicrobial and antioxidant activity. The biosynthesized monodisperse silver nanoparticles and encapsulated silver nanoparticles demonstrated better antimicrobial and antioxidant activity which can be used in various biomedical applications.

scholarly journals Phenolic Content and Antioxidant, Antibacterial Activities of Ethanolic Extract From Lemon Balm and Oregano Plants

2014 ◽  
Vol 11 (1) ◽  
pp. 103-110
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Strong Relationship ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Resistant Isolate ◽  
Lemon Balm

The antioxidant and antibacterial activities of ethanolic extract and phenolic compounds extract of Lemon balm (Melissa officinalis) and Oregano (Oreganum vulgare) plants were studied; the phenolic content and the relationship between these compounds and the above activities were also investigated. The results showed that the Lemon balm had the highest phenolic content (56.5% mg g) and the phenolic content of Oregano was twice lower than Lemon balm. Lemon balm has the highest antioxidant activity which causes lipid peroxidation inhibition activity of linoleic acid (90.5%), this activity was more than ?-tocopherole antioxidant activity (79.3 %). It was found that the main source of antioxidant activity of these plants was belonging to phenolic compounds and the results proved the strong relationship between antioxidant activity and phenolic content. The ethanolic extract of Lemon balm was exhibited strong antibacterial activity ,the inhibition included all bacterial isolates , with highest inhibition zone against Bacillus cereus ( 26 mm), while Oregano did not exhibit clear antibacterial activity, Aeromonas hydrophila was the most resistant isolate . It was obvious from the results of effect of phenolic compounds on bacteria that no relationship between antibacterial activity and phenolic content and the inhibition may be due to other compounds.

scholarly journals Chemical composition, antioxidant and antibacterial activities of the essential oils of Juniperus phoenicea, Juniperus thurifera and Juniperus oxycedrus

2019 ◽  
Vol 9 (3) ◽  
pp. 190-198
Author(s):  
Houda El Hajjouji ◽  
Rachid Rahhal ◽  
Said Gmouh ◽  
Mohammed Hsaine ◽  
Hassan Fougrach ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Juniperus Thurifera ◽  
Juniperus Oxycedrus ◽  
Juniperus Phoenicea

This study aimed to compare the chemical composition, the antioxidant activity and the antibacterial activity of essential oils (EOs) of Juniperus phoenicea, Juniperus thurifera and Juniperus oxycedrus, obtained from Ait Bouguemez region (Province of Azilal, Morocco). The analysis by GC/MS of essential oils led to identify 37, 54 and 38 components for J. phoenicea, J. thurifera, and J. oxycedrus, respectively. Monoterpenic fraction was found predominant in essential oils of the three samples. The DPPH free radical scavenging activity showed that essential oil of J. thurifera has the strongest antioxidant activity with an IC50 value of 12.07 μg/mL. The antibacterial activity showed that S. aureus was more sensitive than P. aeruginosa and E. coli for the three EOs tested.

scholarly journals Green synthesis of silver nanoparticles using Tropaeolum majus: Phytochemical screening and antibacterial studies

2021 ◽  
Vol 10 (1) ◽  
pp. 85-94
Author(s):  
Saud Bawazeer ◽  
Abdur Rauf ◽  
Syed Uzair Ali Shah ◽  
Ahmed M. Shawky ◽  
Yahya S. Al-Awthan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Biological Action ◽  
Klebsiella Pneumonia ◽  
Atomic Force ◽  
Tropaeolum Majus

Abstract The green biosynthesis of metal nanoparticles of already explored phytomedicines has many advantages such as enhanced biological action, increased bioavailability, etc. In this direction, keeping in view the peculiar medicinal value of Tropaeolum majus L., we synthesized its silver nanoparticles (AgNPs) by adopting eco-friendly and cost-effective protocol by using methanolic and aqueous extract of T. majus. The synthesized AgNPs were characterized by using several techniques including UV spectroscopic analysis, FTIR analysis, and atomic force microscopy. The methanolic/aqueous extracts of T. majus and synthesized AgNPs were assessed for antioxidant potential and antimicrobial effect. The preliminary screening showed that the T. majus extracts have variety of reducing phytochemicals including tannins, terpenoids, flavonoids, and cardiac glycosides. The green synthesis of AgNPs was confirmed by the appearance of sharp peak at 430–450 nm in the UV-Visible spectra. The FTIR spectral analysis of extract and AgNPs exhibited that peaks at 2947.23, 2831.50, 2592.33, 2522.89, and 1,411 cm−1 disappeared in the spectra of FTIR spectra of the AgNPs, indicating carboxyl and hydroxyl groups are mainly accountable for reduction and stabilization of AgNPs. Atomic force microscopic scan of the synthesized AgNPs confirmed its cylindrical shape with size of 25 µm. The extracts and AgNPs were investigated for antioxidant potential by DPPH-free radical essay, which showed that aqueous extract has significant and dose-independent antioxidant activity; however, the synthesized AgNPs showed decline in antioxidant activity. The extracts and synthesized AgNPs were also evaluated for antibacterial activity against Klebsiella pneumonia, Staphylococcus aureus, and Bacillus subtilis. Neither extract nor AgNPs were active against Klebsiella pneumonia. The aqueous and methanolic extract exhibited inhibition against Bacillus subtilis and their synthesized AgNPs were active against Staphylococcus aureus. Our data concluded that the extracts of T. majus have necessary capping and reducing agents which make it capable to develop stable AgNPs. The aqueous extract of T. majus has potential antioxidant effect; however, the AgNPs did not enhance its free radical scavenging effect. The bacterial strains’ susceptibility of the extract and AgNPs was changed from Bacillus subtilis to Staphylococcus aureus, respectively. The biological action of AgNPs is changed in case of antibacterial activity which means that AgNPs might change the specificity of T. majus and likewise other drugs.

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