scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF AMOMUM SUBULATUM ROXB. OF NEPAL

2017 ◽  
Vol 10 (4) ◽  
pp. 95
Author(s):  
Bimala Subba ◽  
Tank Raj Seling ◽  
Ram Chandra Kandel ◽  
Ganga Prasad Phuyal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Drug ◽  
Traditional Use ◽  
Low Altitude ◽  
Amomum Subulatum ◽  
Antimicrobial And Antioxidant Activity ◽  
Antibacterial And Antioxidant Activity

Objective: The present study was designed to study and compare the antibacterial and antioxidant activity of methanol extracts of Amomum subulatum Roxb. seeds collected from two different regions of Nepal.Methods: Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl assay and Antibacterial activity was measured by agar well-diffusion method.Results: Results showed that both sample extracts had interesting antibacterial activity against all test microorganisms and had remarkable radical scavenging potential to be used as an antioxidant. Sample B (i.e., collected from high altitude Taplejung district) exerted comparative quit higher antimicrobial and antioxidant activity than the Sample A (i.e., is collected from low altitude Panchthar district). However, the standard antibacterial drug Tetracycline exhibits superior activity than the extracts.Conclusions: The results suggest that both samples (i.e., seed extract of A. subulatum Roxb.) possess almost similar moderate antibacterial and antioxidant activity in comparison to standards which justifies the traditional use of this plant.Keywords: Extract, Cardamom, Activity, Medicinal plants, Seeds, Bacillus pumilus.

scholarly journals Evaluation of the Antibacterial and Antioxidant Activity of Mimosa rubicaulis and Reinwardtia indica

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Roshani Gurung ◽  
Sundar Adhikari ◽  
Kalpana Parajuli
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Antibacterial Activity ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Ic50 Value ◽  
Antibacterial And Antioxidant Activity

Many plants have the property of wound healing, but most of the people are using costly allopathic medicine for the wound. This might be due to lack of awareness about the traditional uses and lack of scientific study of ethnomedicinal plants. So, this study aimed to carry out the antibacterial and antioxidant activity of two medicinal plants which are used traditionally for wound healing activity, i.e.,Mimosa rubicaulis and Reinwardtia indica. Different parts of Mimosa rubicaulis (root, stem, and leaves) and Reinwardtia indica (flower and leaves) were used for the study. Extractions were done by maceration using ethanol as solvent. Antibacterial activity was carried out by the well diffusion method, and antioxidant activities were screened by DPPH radical scavenging and NO scavenging assays. Extract of M. rubicaulis has shown a weak zone of inhibition towards S. aureus and P. vulgaris whereas R. indica has shown no zone of inhibition towards selected bacterial strains. Leaf, root, and stem extracts of M. rubicaulis have shown potent antioxidant activity, i.e., IC50 value of 9.8 µg/ml, 10.19 µg/ml, and, 13.64 µg/ml, respectively. Similarly, leaf extract of R. indica exhibited antioxidant activity with an IC50 value of 19.73 μg/ml. The percentage inhibition of NO radicals of root and stem of M. rubicaulis and leaves of R. indica was 31%, 21%, and 22%, respectively. Out of these two plant species, M. rubicaulis had shown antibacterial activity towards selected microorganism, but antioxidant activity was shown by both plant species. These properties on above mentioned two plant species might help for the development of a marketed formulation for antibacterial and wound-healing agent since wound healing is promoted by antibacterial and antioxidant activities.

scholarly journals Antibacterial and Antioxidant Activities of Slender Amaranth Weed

2020 ◽  
Vol 38 ◽  
Author(s):  
M. AKBAR ◽  
I.N. SHERAZI ◽  
M.S. IQBAL ◽  
T. KHALIL ◽  
H.M. WAQAS
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pseudomonas Syringae ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Root Extract

ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.

scholarly journals Phytochemical, Antibacterial and Antioxidant Activity Evaluation of Rhodiola crenulata

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3664
Author(s):  
Lingyun Zhong ◽  
Lianxin Peng ◽  
Jia Fu ◽  
Liang Zou ◽  
Gang Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethyl Gallate ◽  
Dpph Radical ◽  
Rhodiola Crenulata ◽  
Dpph Radical Scavenging

The chemical components, as well as the antibacterial and antioxidant activities of the essential oil (EO) and crude extracts prepared from Rhodiola crenulata were investigated. The essential oil was separated by hydrodistillation, and gas chromatography-mass spectrometry (GC-MS) was used to identify its constituents. A total of twenty-seven compounds was identified from the EO, and its major components were 1-octanol (42.217%), geraniol (19.914%), and 6-methyl-5-hepten-2-ol (13.151%). Solvent extraction and fractionation were applied for preparing the ethanol extract (crude extract, CE), petroleum ether extract (PE), ethyl acetate extract (EE), n-butanol extract (BE), and water extract (WE). The CE, EE and BE were abundant in phenols and flavonoids, and EE had the highest total phenol and total flavonoid contents. Gallic acid, ethyl gallate, rosavin and herbacetin were identified in the EE. The antibacterial activity results showed that the EO exhibited moderate inhibitory activity to the typical clinic bacteria, and EE exhibited the strongest antibacterial activity among the five extracts. For the compounds, ethyl gallate showed the strongest inhibitory activity to the test bacteria, and its minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value for all the tested bacteria was 0.24 mg/mL and 0.48 mg/mL, respectively. The results of antioxidant activity showed that both CE and EE exhibited strong antioxidant activities in the DPPH radical scavenging and Fe2+ reducing power tests, however, EO showed relatively weaker antioxidant ability. Ethyl gallate and rosavin exhibited excellent activity in the DPPH radical scavenging assay, and their IC50 value was 5.3 µg/mL and 5.9 µg/mL, respectively. Rosavin showed better reduction power activity than the other three compounds. These results could provide more evidence for the traditional use of R. crenulata, and would be helpful for improving its application further.

scholarly journals Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Mampolelo M. Photolo ◽  
Vuyo Mavumengwana ◽  
Lungile Sitole ◽  
Matsobane G. Tlou
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Bacterial Species ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Dpph Assay ◽  
Isolation And Identification ◽  
Antimicrobial And Antioxidant Activity

This study reports on the isolation and identification of Methylobacterium radiotolerans MAMP 4754 from the seeds of the medicinal plant, Combretum erythrophyllum, for the purposes of investigating antimicrobial and antioxidant activities from this endophyte. The strain identity was confirmed by 16S rRNA-based phylogeny and Scanning Electron Microscopy (SEM). Ethyl acetate and chloroform (1 : 1 v/v) extracts from the endophyte were tested for antimicrobial and antioxidant activity on a total of 7 bacterial species (3 Gram-positive and 4 Gram-negative) using the standard Minimum Inhibitory Concentration (MIC) protocol and Quantitative Radical Scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The MICs were recorded at 250 μg/mL for B. subtilis ATCC 19659, B. cereus ATCC 1076, E. coli ATCC1053, and 62.5 μg/mL for K. oxytoca ATCC 13182 and M. smegmatis ATCC 21293, while an IC50 of 5.65 μg/mL was recorded with the DPPH assay. Qualitative phytochemical analysis was positive for alkaloids, flavonoids, and steroids. Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of 9-octadecene, 2,4-dinitrophenyl acetate, and 2(5H)-furanone, which have been previously reported for the targeted activities. M. radiotolerans MAMP 4754 tested positive for antimicrobial and antioxidant activity and this is linked to the production of plant-derived secondary metabolites by this strain.

scholarly journals In Vitro Assessment of Selected Medicinal Plants Used by the Bapedi Community in South Africa for Treatment of Bacterial Infections

2018 ◽  
Vol 23 ◽  
pp. 2515690X1876273 ◽  
Author(s):  
Refilwe Given Kudumela ◽  
Peter Masoko
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Bacterial Infections ◽  
Antioxidant Activities ◽  
Scientific Information ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Limpopo Province ◽  
Total Phenolic

There is very little scientific information on indigenous plants used for medicinal purposes. Therefore, the aim of the study was to evaluate the antibacterial and antioxidant activities of the leaves of Commelina africana, Elephantorrhiza elephantina, Dombeya rotundifolia, and the whole plant excluding the roots of Schkuhria pinnata indigenous medicinal plants from the Limpopo Province, which may be used for the treatment in humans infected with bacterial pathogens. The screening of different phytoconstituents using standard methods revealed the presence of terpenoids, flavonoids, tannins, and saponins. The total phenolic, tannin, and flavonoid content were estimated using spectrophotometric methods. D rotundifolia had the highest amounts of phenol (259.00 ± 2.65 mg of gallic acid equivalent [GAE]/g), tannin (330.33 ± 15.63 mg of GAE/g), and flavonoid (19.90 ± 0.75 mg of quercetin equivalent [QE]/g) content, while S pinnata had the least. All the plants had antibacterial activity against Escherichia coli and Pseudomonas aeruginosa with the bioautography method. The microbroth dilution assay revealed antibacterial activity with minimum inhibitory concentration values raining from 0.02 to 2.50 mg/mL. D rotundifolia had the highest antioxidant activity in both the free radical scavenging and ferric reducing power methods. S pinnata and C africana extracts had high antibacterial activity, while D rotundifolia had high antioxidant activity, which made them potential plants for isolation of bioactive compounds and possible application in the pharmaceutical industry.

scholarly journals Antibacterial and antioxidant activity of different extracts obtained from Withania somnifera (Ashwagandha)

2021 ◽  
Vol 12 (4) ◽  
pp. 873-877
Author(s):  
Kubukgolla W W ◽  
Attanayake A M H S ◽  
Jayaweera J A A S
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Commensal Bacteria ◽  
Root Extract ◽  
Open Market ◽  
Candida Sp ◽  
Antibacterial And Antioxidant Activity ◽  
Different Parts

Ashwagandha (W. somnifera) is widely known as the queen of Indian Ayurveda. The objective of this study was to determine the antioxidant and antibacterial activity of the different parts of the Ashwagandha.  The antioxidant activity of different extracts from different parts of Ashwagandha was determined using DPPH free radical scavenging method. Ashwaghanda root inhabiting bacteria was isolated using pour-plate technique. The agar well method and filter paper method were followed to assess the antibacterial activity of the extracts. Ethanol-water extracts (1:1 v/v) gave higher yield of extractable matter than methanol extracts. The antioxidant activity of fresh root extract was significantly higher than other parts of the plant (stem and leaves) as well as the plant material bought from open market. The minimal inhibitory concentration (MIC) values of Ethanol-water extract against MRSA, Candida Sp. and Salmonella Sp. were >256 mg/L. The commensal bacteria (Bacilli Sp.) inhabiting Ashwagandha roots displayed mild antibacterial activity against pathogenic P. aeruginosa. Keywords:  Ashwaghanda, commensal bacteria, Antibacterial activity, Antioxidant activity, Crude extracts

scholarly journals Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia)

2017 ◽  
Vol 9 (1) ◽  
pp. 134
Author(s):  
Mardho Tillah ◽  
Irmanida Batubara ◽  
Rita Kartika Sari
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Positive Control ◽  
Diffusion Method ◽  
Pinus Merkusii

<p class="Abstract">The most common human pathogen that colonizes in a third of healthy people around the world are <em>Staphylococcus aureus</em>, and one of the materials allegedly able to overcome the pathogen is resin. Resin has been used in folk medicine for thousands of years to treat diseases. The antimicrobial activity of natural resins can be associated with a variety of organic compounds contained in them such as diterpenoids and triterpenoids. This research aimed to explore the antibacterial and antioxidant activities of <em>Pinus merkusii, P. oocarpa, P. insularis, Agathis loranthifolia</em> resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using <em>n</em>-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples determined using the disc diffusion method against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. The results showed that the yield of resin extract was ranging from 8.44 % to 95.56%. All extracts and essential oil could not inhibit <em>E coli </em>growth but inhibit the <em>S. aureus </em>growth. This experiment concluded that resin <em>n</em>-hexane extract from <em>P.oocarpa</em> was the most potent as antibacterial activity against <em>S. aureus</em>. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid. Result of this study show that pine resin from Indonesia has potential as an antibacterial agent. </p>

scholarly journals Antibacterial and Antioxidant Activities of Centeurea damascena Methanolic Extract

2019 ◽  
Vol 5 (1) ◽  
pp. 55-63
Author(s):  
Mohammad Jaafreh ◽  
Khaled Khleifat ◽  
Haitham Qaralleh ◽  
Muhamad Al-limoun
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Micrococcus Luteus ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
The Family

The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial and antioxidant activity as well as its toxicity. The aims of the study were to determine the antimicrobial and antioxidant activities and toxicity of methanolic plant extracts of Centaurea damascene. The methanolic extracts were screened for their antibacterial activity against nine bacteria (Staphylococcus aureus ATCC 43300, Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 10240, and Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 11293, Pseudomonas aerugino and Klebsiella pneumoniae, Enterobacter aerogenes ATCC 13048 and Salmonella typhi ATCC19430). The antibacterial activity was assessed by using the disc diffusion methods and the minimum inhibition concentrations (MIC) using microdilution method. The extracts from Centaurea damascene possessed antibacterial activity against several of the tested microorganisms. The MIC of methanol extract of C. damascene ranged from 60–1100 µg/mL. Free radical scavenging capacity of the C. damascena methanol extract was calculated by DPPH and FRAP test. DPPH radicals were scavenged with an IC50 value of 17.08 µg /ml. Antioxidant capacities obtained by the  FRAP was 51.9 and expressed in mg Trolox g-1 dry weight. The total phenolic compounds of the methanol extracts of aerial parts, as estimated by Folin–Ciocalteu reagent method, was about 460 mg GAE/ g. The phenolic contents in the extracts highly correlate with their antioxidant activity, (R2 = 0.976) confirming that the antioxidant activity of this plant extracts is considerably phenolic contents-dependent

scholarly journals Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

2019 ◽  
Vol 14 (2) ◽  
pp. 73
Author(s):  
Wendy Alexander Tanod ◽  
Didit Kustantio Dewanto ◽  
Samliok Ndobe ◽  
Putut Har Riyadi ◽  
Masteria Yunovilsa Putra
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Moderate Activity ◽  
Soft Corals ◽  
Strong Activity ◽  
E Coli ◽  
Central Sulawesi

This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.

scholarly journals ANTIBACTERIAL, IN VITRO CYTOTOXIC, AND ANTIOXIDANT ACTIVITIES OF ELECTROLYZED OXIDIZING/REDUCING WATER

2019 ◽  
pp. 40-43
Author(s):  
ASMIYENTI DJALIASRIN DJALIL ◽  
ELZA SUNDHANI ◽  
RETNO WAHYUNINGRUM ◽  
DWI HARTANTI ◽  
NONI LESTIOWATI ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
T47d Cell ◽  
Human Breast ◽  
Potential Health ◽  
Electrolyzed Water ◽  
Diphenyl Tetrazolium Bromide

Objective: Electrolyzed oxidizing/reducing water is popular as health beneficial water in Indonesia. In this study, we examined the level of antibacterial,anticancer, and antioxidant activity of the electrolyzed water.Methods: The efficacy of electrolyzed water produced by Enagic® at six level pH (2.5, 6.0, 7.0, 8.5, 9.0, and 9.5) was investigated. Antibacterialactivity was evaluated using a macrodilution method. The anticancer activity was performed against human breast cancer (T47D) cell lines using3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Moreover, the antioxidant activity was determined using antioxidant model,2,2-diphenyl-1picrylhydrazyl radical scavenging activity.Results: The results show that electrolyzed water exhibited antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis.Among six level pH, electrolyzed water at pH 2.5 showed the highest antibacterial activity. The in vitro cytotoxic activity of electrolyzed water showedpotential moderate cytotoxicity. The activity tends to be higher in alkaline electrolyzed water. However, the electrolyzed water showed free radicalscavenging activity.Conclusion: Electrolyzed water that marked in Indonesia has some potential health benefits. The activity is dependent on pH.

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