EGFR EXPRESSION IN PRIMARY EPITHELIAL URINARY BLADDER TUMORS AND ITS CORRELATION WITH CLINICOPATHOLOGICAL PARAMETERS

2021 ◽  
pp. 44-47
Author(s):  
Deepika Sharma ◽  
Sudarshan Kumar Sharma ◽  
Pamposh Raina ◽  
Anchana Gulati
Keyword(s):  
Urinary Bladder ◽  
Histological Grade ◽  
Urinary Bladder Cancer ◽  
Clinicopathological Parameters ◽  
Female Size ◽  
Novel Therapy ◽  
Chi Square ◽  
Cross Sectional ◽  
Urothelial Carcinomas ◽  
Egfr Expression

Introduction: EGFR is over expressed in many epithelial tumors including urothelial carcinomas. Over expression of EGFR is considered as a poor prognostic marker in various studies. Thus, the present study was done to evaluate the EGFR expression in primary urothelial carcinomas and its correlation with clinicopathological parameters. Methods: This was a cross-sectional, observational study carried out between June 1, 2019 to May 31, 2020 in the Department of Pathology and Urology, Indira Gandhi Medical College, Shimla. One hundred and ninety seven patients with primary epithelial urinary bladder cancer were included. Patients with inammatory and metastatic lesions of urinary bladder and post chemoradiotherapy were excluded. The correlation between EGFR expression and the various factors like age (<60 years or ≥60 years), sex (male/female), size of tumor (< 3cm or ≥ 3cm), number of tumors (solitary/multiple) and grade (high/low) were evaluated using EpiInfoV.7 software version and chi-square test. Results: The age of patients ranged from 36 to 89 years. Male preponderance was observed. Most common clinical presentation was painless hematuria. EGFR positivity was observed in majority, 190 cases, irrespective of histological type and grade of tumor. No statistical signicant correlation was found between EGFR expression and age of patient, size of tumor, number of tumor and histological grades of urothelial tumors. Conclusion: Majority of urothelial carcinomas over expressed EGFR irrespective of their histological grade. So, targeted EGFR therapy in urothelial carcinomas can emerge as a novel therapy improving overall survival and prognosis of the patients with urothelial carcinomas.

scholarly journals Immunohistochemical Investigation of HER/AKT/mTOR Pathway and Cellular Adhesion Molecules in Urothelial Carcinomas

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Nikolaos Koletsas ◽  
Triantafyllia Koletsa ◽  
Spyros Choidas ◽  
Konstantinos Anagnostopoulos ◽  
Stavros Touloupidis ◽  
...  
Keyword(s):  
Adhesion Molecules ◽  
Urinary Bladder Cancer ◽  
Cellular Adhesion ◽  
Mtor Pathway ◽  
Clinicopathological Parameters ◽  
Cancer Tissue ◽  
Her2 Overexpression ◽  
High Grade ◽  
Cellular Adhesion Molecules ◽  
Urothelial Carcinomas

Background. Several investigators have suggested the possibility that the expression of both EGFR and HER2 could be utilized for molecularly targeted therapy in urinary bladder cancer. We tried to evaluate the expression of HER2 and EGFR and activation of the AKT/PTEN/mTOR pathway in urothelial carcinomas and if there is any association between them and cellular adhesion molecules (CAMs). Materials and Methods. Forty-one paraffin-embedded urothelial cancer tissue blocks were collected. Immunostains for HER2, EGFR, MIB1, phospho-AKT, PTEN, phospho-mTOR, e-cadherin, p-cadherin, and b-catenin were performed on tissue microarrays sections. The immunohistochemical results were correlated with clinicopathological parameters. Results. The overexpression of HER2 was found in 19.6% of the cases and it was associated with high grade tumors with a high mitotic index and phosphorylation of AKT and mTOR. Muscle-invasive tumors presented both cytoplasmic and nuclear losses of PTEN expression. There was no association between HER/AKT/mTOR pathway activation and CAM expression. Although cadherins were often coexpressed, only p-cadherin immunoreactivity was associated with tumor grade and high proliferative index. Conclusions. HER2 overexpression is found in a respective proportion of urothelial carcinomas. P-cadherin expression is associated with high grade UCs but it is not affected by HER2 overexpression or by activation of HER/AKT/mTOR pathway.

scholarly journals EGFR-expression in primary urinary bladder cancer and corresponding metastases and the relation to HER2-expression. On the possibility to target these receptors with radionuclides

2015 ◽  
Vol 49 (1) ◽  
pp. 50-58 ◽  
Author(s):  
Jörgen Carlsson ◽  
Kenneth Wester ◽  
Manuel De La Torre ◽  
Per-Uno Malmström ◽  
Truls Gårdmark
Keyword(s):  
Bladder Cancer ◽  
Urinary Bladder ◽  
Kinase Inhibitors ◽  
Urinary Bladder Cancer ◽  
Distant Metastases ◽  
Whole Body ◽  
Minor Effect ◽  
Her2 Expression ◽  
Primary Tumors ◽  
Egfr Expression

Abstract Background. There is limited effect of tyrosine kinase inhibitors or “naked” antibodies binding EGFR or HER2 for therapy of metastasized urinary bladder cancer and these methods are therefore not routinely used. Targeting radionuclides to the extracellular domain of the receptors is potentially a better possibility. Methods. EGFR- and HER2-expression was analyzed for primary tumors and corresponding metastases from 72 patients using immunohistochemistry and the internationally recommended HercepTest. Intracellular mutations were not analyzed since only the receptors were considered as targets and intracellular abnormalities should have minor effect on radiation dose. Results. EGFR was positive in 71% of the primary tumors and 69% of corresponding metastases. Local and distant metastases were EGFR-positive in 75% and 66% of the cases, respectively. The expression frequency of HER2 in related lesions was slightly higher (data from previous study). The EGFR-positive tumors expressed EGFR in metastases in 86% of the cases. The co-expression of EGFR and HER2 was 57% for tumors and 53% for metastases. Only 3% and 10% of the lesions were negative for both receptors in tumors and metastases, respectively. Thus, targeting these receptors with radionuclides might be applied for most patients. Conclusions. At least one of the EGFR- or HER2-receptors was present in most cases and co-expressed in more than half the cases. It is therefore interesting to deliver radionuclides for whole-body receptor-analysis, dosimetry and therapy. This can hopefully compensate for resistance to other therapies and more patients can hopefully be treated with curative instead of palliative intention.

Clinicopathological study of urothelial neoplasms in urinary bladder with special reference to expression of Her2/neu and Ki-67 in malignant lesions

2021 ◽  
Vol 8 (3) ◽  
pp. 378-385
Author(s):  
Sarmila Sen ◽  
Ayesha Abid ◽  
Ranjana Bandyopadhyay
Keyword(s):  
Urothelial Carcinoma ◽  
Histological Grade ◽  
Tertiary Care ◽  
Population Group ◽  
Low Grade ◽  
Care Hospital ◽  
Ki 67 ◽  
Cross Sectional ◽  
Non Invasive ◽  
Urothelial Carcinomas

Urothelial carcinomas are the fourth most common tumors worldwide. Studies have shown that Her2/neu is associated with increased tumour grade and Ki‐67 is related to tumour recurrence and stage progression. As no such study has been undertaken is this region, the present study was aimed to establish Her2/neu and Ki-67 as novel prognostic markers of urothelial neoplasms.: To evaluate the clinical profile of urothelial neoplasms and correlate the expression of Her2/neu and Ki-67 in urothelial carcinomas.Observational and cross-sectional study (January 2018-May 2019) in a tertiary care hospital in Eastern India.: 40 specimens of urothelial neoplasms were studied. Clinical history of patients was collected. Microscopic examination was done to assess tumour stage and histological grade. Immunohistochemistry with Her2/neu and Ki-67 was performed.: Statistical Package for the Social Sciences SPSS (version 20.0, IBM, Chicago, IL, USA) for windows software.: 8 cases were diagnosed as low grade non-invasive urothelial carcinoma, 31 cases as infiltrating urothelial carcinoma and 1 case as papillary urothelial neoplasm of low malignant potential (PUNLMP). 96.8% of infiltrating carcinomas and 87.5% of low grade non-invasive carcinomas showed Ki-67 positivity. Moderate-to-strong Her2/neu overexpression (2+ or 3+) was observed in only 21% of the cases.: The expression of Ki-67 increased with increase in the grade of the tumour which was suggestive of the prognostic importance of Ki-67. Her2/neu positivity was seen in infiltrating cases. No significant association between the tumor grade and Her2/neu expression, indicated the need for further studies with a larger population group.

scholarly journals Angiodrastic Chemokines in Colorectal Cancer: Clinicopathological Correlations

2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
George Emmanouil ◽  
George Ayiomamitis ◽  
Adamantia Zizi-Sermpetzoglou ◽  
Maria Tzardi ◽  
Andrew Moursellas ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Clinical Stage ◽  
Clinicopathological Parameters ◽  
Cancer Tissue ◽  
Colorectal Tumors ◽  
Poor Survival ◽  
Chi Square ◽  
Strong Expression ◽  
Egfr Expression ◽  
Clinicopathological Correlations

Aim. To study the expression of angiodrastic chemokines in colorectal tumors and correlate findings with clinicopathological parameters and survival.Methods. The proangiogenic factor VEGF, the angiogenic chemokines CXCL8 and CXCL6, and the angiostatic chemokine CXCL4 were measured by ELISA in tumor and normal tissue of 35 stage II and III patients and correlated with the histopathology markers Ki67, p53, p21, bcl2, EGFR, and MLH1 and 5-year survival. The Wilcoxon and chi-square tests were used for statistical comparisons.Results. There was a significant increase of CXCL6 (p=0.005) and VEGF (p=0.003) in cancerous tissue compared to normal. Patients with lower levels of CXCL8 and CXCL4 lived significantly longer. Patients with loss of EGFR expression had higher levels of CXCL8 while p21 loss was associated with higher levels of CXCL6. Chemokine levels were not correlated with TNM or Dukes classification. Strong expression of p53 was accompanied by decreased survival.Conclusions. (1) The angiogenic factors CXCL6 and VEGF are increased in colorectal cancer tissue with no association with the clinical stage of the disease or survival. (2) However, increased levels of tissue CXCL8 and CXCL4 are associated with poor survival. (3) Strong expression of p53 is found in patients with poor survival.

scholarly journals Breast carcinoma: A clinicopathological study of 90 cases.

2020 ◽  
Vol 27 (02) ◽  
pp. 381-387
Author(s):  
Iram Asrar ◽  
M. Usman ◽  
Saira Javeed ◽  
Ammara Anwar ◽  
Nadia Naseem ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Tumor Size ◽  
Histological Grade ◽  
Local Population ◽  
Menopausal Status ◽  
Tertiary Care ◽  
P Value ◽  
Histological Grading ◽  
Chi Square ◽  
Cross Sectional

Objective: To describe the clinicopathological features of breast carcinoma in the local population based on age, menopausal status, laterality, tumor site, tumor size, histological subtype, and histological grading of breast carcinoma. Study Design: Descriptive cross-sectional study. Setting: University of Health Sciences (U.H.S), Lahore. Period: 6 months (June 2017-Dec 2017) after approval from the ethical committee of U.H.S. Material & Methods: In this study, the clinicopathological profile of 90 female patients of breast carcinoma was evaluated from different tertiary care hospitals and U.H.S, Lahore. Histological diagnosis and histological grading was then carried out and were analyzed statistically through SPSS 21.0.  Associations between the histological grade and other parameters were established using the Chi-square test. Results: The mean age of patients was 48.44±11.64 with 33.3% of patients younger than 40 years of age and 58.9% of women were found pre-menopausal. The left breast was more involved (65.6%) than the right side, upper outer quadrant of the breast was the commonest site (64.4%), and the majority of the tumors (76%) were more than 2 cm in size. All 90 cases were histologically diagnosed as invasive carcinoma of no special type (NST). Nottingham histological grade-II was observed as the commonest grade (54.4%) followed by grade- III (31.1%). Histological grade was significantly associated with the tumor size (p-value=0.000, Chi-square value= 29.17). Conclusions: Breast cancer is mostly reported in younger premenopausal women in our region. Delay in the diagnosis is most likely related to larger tumor sizes and moderate to poor histological grades.

Personal Audio System: Hearing Symptoms, Habits, and Sound Pressure Levels Measured in Real Ear and a Manikin

2020 ◽  
Vol 63 (6) ◽  
pp. 2016-2026
Author(s):  
Tamara R. Almeida ◽  
Clayton H. Rocha ◽  
Camila M. Rabelo ◽  
Raquel F. Gomes ◽  
Ivone F. Neves-Lobo ◽  
...  
Keyword(s):  
Sound Pressure ◽  
Daily Basis ◽  
Cross Sectional Study ◽  
Normal Hearing ◽  
Chi Square ◽  
Cross Sectional ◽  
The Real ◽  
Significant Difference ◽  
Sound Pressure Levels ◽  
Audio System

Purpose The aims of this study were to characterize hearing symptoms, habits, and sound pressure levels (SPLs) of personal audio system (PAS) used by young adults; estimate the risk of developing hearing loss and assess whether instructions given to users led to behavioral changes; and propose recommendations for PAS users. Method A cross-sectional study was performed in 50 subjects with normal hearing. Procedures included questionnaire and measurement of PAS SPLs (real ear and manikin) through the users' own headphones and devices while they listened to four songs. After 1 year, 30 subjects answered questions about their usage habits. For the statistical analysis, one-way analysis of variance, Tukey's post hoc test, Lin and Spearman coefficients, the chi-square test, and logistic regression were used. Results Most subjects listened to music every day, usually in noisy environments. Sixty percent of the subjects reported hearing symptoms after using a PAS. Substantial variability in the equivalent music listening level (Leq) was noted ( M = 84.7 dBA; min = 65.1 dBA, max = 97.5 dBA). A significant difference was found only in the 4-kHz band when comparing the real-ear and manikin techniques. Based on the Leq, 38% of the individuals exceeded the maximum daily time allowance. Comparison of the subjects according to the maximum allowed daily exposure time revealed a higher number of hearing complaints from people with greater exposure. After 1 year, 43% of the subjects reduced their usage time, and 70% reduced the volume. A volume not exceeding 80% was recommended, and at this volume, the maximum usage time should be 160 min. Conclusions The habit of listening to music at high intensities on a daily basis seems to cause hearing symptoms, even in individuals with normal hearing. The real-ear and manikin techniques produced similar results. Providing instructions on this topic combined with measuring PAS SPLs may be an appropriate strategy for raising the awareness of people who are at risk. Supplemental Material https://doi.org/10.23641/asha.12431435

777: Tissue Microarray Analysis of pAkt, PTEN and L-Plastin in Urinary Bladder Cancer

2005 ◽  
Vol 173 (4S) ◽  
pp. 211-211
Author(s):  
Loleta D. Harris ◽  
Tomasz Tuziak ◽  
Jorge De Lo Cerda ◽  
Anita L. Sabichi ◽  
Ying Yang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urinary Bladder ◽  
Tissue Microarray ◽  
Microarray Analysis ◽  
Urinary Bladder Cancer ◽  
Tissue Microarray Analysis

Oscillation between identities and Subjective psychological health objectives among an informed audience in therapeutic patient education

2018 ◽  
Vol 10 (1) ◽  
pp. 10202
Author(s):  
Sandrine Roussel ◽  
Alain Deccache ◽  
Mariane Frenay
Keyword(s):  
Patient Education ◽  
Psychological Health ◽  
Healthcare Professionals ◽  
Cross Sectional Survey ◽  
Chi Square ◽  
Cross Sectional ◽  
Therapeutic Patient Education ◽  
French Speaking ◽  
Health Objectives ◽  
Self Administered Questionnaire

Introduction: The implementation of Therapeutic Patient Education (TPE) remains a challenge. An exploratory study highlighted two tendencies among practitioners of TPE, which could hamper this implementation: an oscillation between identities (as caregivers versus as educators) and an inclination towards subjective psychological health objectives. Objectives: To verify whether these tendencies can be observed among an informed audience in TPE. Next, to explore the variables associated with one or other of these tendencies. Method: A quantitative cross-sectional survey by a self-administered questionnaire was carried out among 90 French-speaking healthcare professionals. Statistical analyses (chi-square, logistic regression) were then conducted. Results: Sixty percent of respondents displayed identity oscillation, which was found to be linked to task oscillation, patient curability, scepticism towards medicine and practising in France. Fifty-six percent pursued subjective psychological health objectives, which was found to be associated with health behaviour objectives and a locus of power in the healthcare relationship distinct from those seen in the pre-existing health models (biomedical, global). This tendency seems to constitute an alternative model of TPE. Discussion & conclusion: Identity oscillation and subjective psychological health objectives can be both observed. This study stresses the need to deliberate on the form(s) of TPE that is/are desired.

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