PROLIFERATIVE POTENTIALITY OF AMELOBLASTOMA BY EXPRESSION OF Ki-67

2021 ◽  
pp. 29-30
Author(s):  
Debeswar Das ◽  
Priya Shree ◽  
Kumar Vishwajit ◽  
Anupama Kumari ◽  
Satyendra Prasad Yadav
Keyword(s):  
Prognostic Marker ◽  
Oral Pathology ◽  
The Other ◽  
Odontogenic Tumor ◽  
Incisional Biopsy ◽  
Normal Epithelium ◽  
Ki 67 ◽  
Positive Correlation ◽  
Orofacial Region ◽  
Hematoxylin And Eosin

Ameloblastoma is a type of Odontogenic tumor, commonly encountered in orofacial region. It is very aggressive in nature with potential features of recurrence. Ki -67 is already a proven proliferative marker, which is used in our study to assess the proliferative potentiality of Ameloblastoma. Incisional biopsy was done from 10 cases of Ameloblastoma and 5 normal cases as control, at the department of Oral pathology and Microbiology, BIDSH, Patna. Each specimen was processed and two sections were prepared and stained with hematoxylin and eosin and Ki-67 immunohistochemical marker. Positive stained cells with with Ki-67 in ameloblastoma along with normal epithelium were counted from four randomly selected areas and were quantied using a microscope at 400x magnication. Positive correlation seen with Ki-67 positive cells and percentages, which indicates the value of one variable increases, the other also increases. The overall conclusion is that Ki-67 expression can be used as a prognostic marker in Ameloblastoma.

scholarly journals Correlation of 18F-FDG PET/CT SUVmax with clinical features, D-dimer and LDH in patients with primary intestinal lymphoma

2021 ◽  
Vol 49 (7) ◽  
pp. 030006052110298
Author(s):  
Shuo Zhou ◽  
Wenxin Chen ◽  
Meifu Lin ◽  
Guobao Chen ◽  
Cailong Chen ◽  
...  
Keyword(s):  
Fdg Pet ◽  
Clinical Staging ◽  
Imaging Features ◽  
Intestinal Lymphoma ◽  
Ki 67 ◽  
Positive Correlation ◽  
Pet Ct ◽  
18F Fdg ◽  
Fdg Pet Ct ◽  
D Dimer

Objective To investigate the characteristics of fluorine-18-deoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) maximum standardized uptake value (SUVmax) in primary intestinal lymphoma (PIL) and its correlation with D-dimer and lactate dehydrogenase (LDH). Methods Fifty-two patients diagnosed with PIL from June 2016 to December 2019 were analyzed. All patients underwent 18F-FDG PET/CT. The relationships between SUVmax and different pathological subtypes, clinical stages and risk grades were analyzed. The correlations between SUVmax and Ki-67, LDH and D-dimer were determined. Additionally, PET/CT imaging results were collected from 35 patients with primary intestinal cancer (PIC) and compared with the imaging features of PIL. Results SUVmax was significantly different between PIL and PIC groups and various PIL pathological subgroups. Patients in the high-risk PIL group had markedly higher SUVmax values than the intermediate-risk and low-risk groups. A significant positive correlation was observed between SUVmax and Ki-67 in patients with PIL. SUVmax was significantly different between the elevated and normal D-dimer groups. D-dimer showed a positive correlation with SUVmax. Conclusion 18F-FDG PET/CT SUVmax reflects the aggressiveness of lymphoma to a certain degree, is correlated with Ki-67 and determines the risk grades of PIL. Moreover, it facilitates differential diagnosis, clinical staging and treatment based on D-dimer levels.

scholarly journals Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients

2016 ◽  
Vol 38 (2) ◽  
pp. 635-645 ◽  
Author(s):  
Wei Lin ◽  
Shu-hui Dai ◽  
Tao Chen ◽  
Nobuyuki Kawai ◽  
Keisuke Miyake ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Significant Positive Correlation ◽  
Tumor Grade ◽  
Molecular Therapy ◽  
Ki 67 ◽  
Positive Correlation ◽  
Flt Pet ◽  
Pet Tracer ◽  
Positron Emission ◽  
Regulation Functions

Background/Aims: The nucleolar 58-kDa microspherule protein (MSP58) has important transcriptional regulation functions and plays a crucial role in the tumorigenesis and progression of cancers. 3'-deoxy-3'-[18F]fluorothymidine (FLT) has emerged as a promising positron emission tomography (PET) tracer for evaluating tumor malignancy and cell proliferation. Methods: In the present study, the expression of MSP58 was evaluated by immunohistochemistry and the corresponding PET image was examined using FLT-PET in 55 patients with various grades of gliomas. Results: The immunoreactivity score (IRS) of MSP58 increased with tumor grade with grade IV gliomas exhibiting the highest expression and showed a highly significant positive correlation with the Ki-67 index (r = 0.65, P < 0.001). The IRS of MSP58 in the tumor showed a highly significant positive correlation with corresponding FLT uptake value (r = 0.61, P < 0.001). The correlation between MSP58 expression and glioma malignancy was also confirmed by immunofluorescence, RT-PCR and western blot analysis. FLT uptake value also exhibited a highly significant positive correlation with the Ki-67 index (r = 0.59, P < 0.001). Kaplan-Meier analysis revealed that MSP58 expression has a significant prognostic ability for the overall survival time similar to that found in the uptake value of FLT-PET. Conclusion: These results indicate that MSP58 plays an important role in cell proliferation and will be one of the potential candidates of molecular therapy targeting proliferation. FLT-PET might be used as an early measure of treatment response in the proliferation-targeted therapy.

SERUM GONADOTROPHINS, TESTOSTERONE AND SPERMATOGENESIS IN SUBFERTILE MEN

1977 ◽  
Vol 86 (3) ◽  
pp. 651-658 ◽  
Author(s):  
J. H. Aafjes ◽  
J. C. M. van der Vijver ◽  
R. Docter ◽  
P. E. Schenck
Keyword(s):  
Partial Correlation ◽  
Sperm Count ◽  
Correlation Coefficients ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
The Other ◽  
Sperm Production ◽  
Positive Correlation ◽  
Natural Logarithm ◽  
Biopsy Score

ABSTRACT In 210 subfertile men there existed a significant positive correlation between serum FSH and LH (0.41). No correlation was observed between the gonadotrophin levels and testosterone. In contrast to this FSH as well as LH were negatively correlated with the natural logarithm (In) of the sperm count/ml ejaculate (−0.44 and −0.18, respectively). When the positive correlation which existed between FSH and LH was used to calculate partial correlation coefficients, the coefficient between FSH and ln sperm count did hardly change (−0.41) the coefficient between LH and ln sperm count on the other hand became insignificant (−0.05). This suggests that spermatogenesis influences FSH serum levels in subfertile men by a decreased suppression when sperm production is diminished. Testicular biopsies taken from 97 of these patients were used to determine biopsy scores. These scores showed a significant negative correlation with FSH (−0.34) and a positive one with ln sperm count/ml ejaculate (0.45). Interestingly the biopsy score of 16 patients who fertilized their wives, was found to be higher compared with the score of the other patients who did not fertilize. The number of sperm/ml ejaculate and the FSH values of these 2 groups of biopsied patients were, however, not significantly different. This leads to the conclusion that the biopsy score is a better parameter for the evaluation of oligospermic men than either sperm count or FSH serum values.

Morpho-Biochemical Characteristics of Brinjal Germplasms Affect the Abundance And Infestation of Jassid

2021 ◽  
Vol 19 (1) ◽  
pp. 81-91
Author(s):  
F Yasmin ◽  
MR Amin ◽  
M Afroz ◽  
MAH Swapon ◽  
MM Hossain
Keyword(s):  
Protein Content ◽  
Chlorophyll Content ◽  
Negative Correlation ◽  
Significant Positive Correlation ◽  
Significant Negative Correlation ◽  
Reducing Sugar ◽  
Total Sugar ◽  
The Other ◽  
Biochemical Characteristics ◽  
Positive Correlation

The tropical vegetable, brinjal is cultivated throughout the year in Bangladesh but the crop is infested by a number of insects including jassid. This study investigated the effects of different biophysical and biochemical characteristics of twelve brinjal germplasms on the abundance and infestation of jassid. The experiment was conducted during September 2018 to March 2019 in Gazipur, Bangladesh with twelve brinjal germplasms namely BD-7320, BD-7328, BD-9952, BD-10154, BD-10158, BARI Begun-1, BARI Begun-4, BARI Begun-5, BARI Begun- 6, BARI Begun-7, BARI Begun-8 and BARI Begun-9. The incidence of jassid was recorded from 3rd December to 26th March, and the lowest population was found on BARI Begun- 6. Among the biophysical traits, plant height, number of branch and leaf per plant, and leaf area exerted significant positive correlation; whereas trichome on lower and upper surfaces of leaf, spine per stem and leaf showed significant negative correlation with the abundance of jassid. On the other hand, moisture, reducing sugar, total sugar and protein content of the leaves and fruits, chlorophyll content of the leaves exerts significant positive correlation but ash and pH contents of the leaves and fruits were negatively correlated with the abundance of jassid. Jassids showed the lowest level of leaf infestation on BARI Begun-6, which could be for further analysis to develop jassid resistant brinjal. SAARC J. Agric., 19(1): 81-91 (2021)

To Determine the Degree of Agreement between Ki67 and Histopathology to Differentiate between Hydatidiform Mole

2021 ◽  
Vol 15 (10) ◽  
pp. 2619-2621
Author(s):  
Mariya Manzoor ◽  
Haseeb Ahmed Khan ◽  
Sabiha Riaz ◽  
Imrana Tanvir ◽  
Atiya Batool Gardezi ◽  
...  
Keyword(s):  
Hydatidiform Mole ◽  
P Value ◽  
Kappa Statistics ◽  
Cross Sectional Survey ◽  
Ki 67 ◽  
Cross Sectional ◽  
Histopathologic Diagnosis ◽  
Hematoxylin And Eosin ◽  
High Degree ◽  
Gross Examination

Aim: To determine the degree of agreement between Ki67 and histopathology to differentiate between hydatidiform mole and hydropic abortus. Methods: Descriptive, Cross Sectional Survey was conducted in the Department of Pathology, Fatima Memorial Hospital, Lahore during 6 months (May 11, 2016 to Nov 11, 2016). Using non-probability consecutive sampling, 105 cases were included in this study as per the inclusion and exclusion criteria. 10% buffered formalin was used to fix the sepecimens. Gross examination and staining with Hematoxylin and Eosin was done. The cases were diagnosed by a histopathologist. IHC staining Ki67 was performed, assessed by histopathologist and the data was recorded on the proforma. Results: The mean age was 27.79±5.81 years with minimum and maximum ages of 18 years and 42 years respectively. The histopathological findings showed hydatidiform mole in 41 (39%) women and hydropic abortus in 64(61%) women. The Ki67 was reported to be >25 in 41(39%) patients and less than or equal to 25 in 64(61%) patients. The agreement of differentiation in the two types for histopathology and Ki67 was found in 101(96.2%) patients. Kappa statistics showed 92% (p-value= 0.000) strength of agreement between histopathology and Ki67. Conclusion: There is high degree of agreement between Ki67 and histopathology for differentiation of hydatidiform mole and hydropic abortus. So Ki67 can be used as an adjacent in histopathologic diagnosis of hydatidiform mole in difficult cases. MeSh words: Hydatidiform Mole, Immunohistochemistry, Ki-67 Antigen

Risk of simultaneous phenotypic expression of hip and elbow dysplasia in dogs

2010 ◽  
Vol 23 (01) ◽  
pp. 28-30 ◽  
Author(s):  
T. Cachon ◽  
D. Remy ◽  
C. Carozzo ◽  
E. Viguier ◽  
P. Maitre ◽  
...  
Keyword(s):  
Risk Ratio ◽  
Phenotypic Expression ◽  
The Other ◽  
Phenotypic Correlation ◽  
Positive Correlation ◽  
Elbow Dysplasia

SummaryIn order to look for phenotypic correlation between hip (HD) and elbow (ED) dysplasia, we used radiographic scoring obtained from 1,411 dogs of different breeds, which were evaluated for authoritative grading of both conditions. In this population, we found that the risk ratio for an animal to be simultaneously affected by HD and ED is 1.67. For a dog with ED, the risk ratio to be affected by HD increases as the ED grade increases. Similarly, for a dog affected by HD, the risk ratio to be affected by ED increases as the HD grade increases. In a dog affected by HD or ED, the clinician should look for the second condition in the same animal. Due to the low, yet positive correlation, selection against one trait will not affect the other trait sufficiently. Therefore selection has to be conducted at reduction of HD as well as reduction of ED.

The Sucrose-Natural Gypsum Makes the Compound Retarder to the Cement and the Concretes Performance Influence Experimental Study

2011 ◽  
Vol 335-336 ◽  
pp. 1219-1222
Author(s):  
Ru Qin Wang
Keyword(s):  
Experimental Study ◽  
Physical Properties ◽  
Experimental Research ◽  
Mixing Time ◽  
The Other ◽  
Positive Correlation ◽  
Other Hand ◽  
Cement And Concrete

By the experimental research to the effect sucrose-natural gypsum as composite retarder on the performance of cement and concrete. This paper analyzes and summarizes the fact that the influence of this kind of composite retarder is more obvious than the slow setting effect of a single sugar or plaster. And the retardation of cement and concrete the mixing time is basically the amount of sugar a positive correlation. And on the other hand, it will not influence other physical properties.

Expression of heat shock proteins in medulloblastoma

2013 ◽  
Vol 12 (5) ◽  
pp. 452-457 ◽  
Author(s):  
George A. Alexiou ◽  
George Vartholomatos ◽  
Kalliopi Stefanaki ◽  
Amalia Patereli ◽  
Lefkothea Dova ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Significant Positive Correlation ◽  
Large Cell ◽  
Hsp70 Expression ◽  
Ki 67 ◽  
Positive Correlation ◽  
Wide Range ◽  
Shock Proteins ◽  
Multiplex Bead

Object Medulloblastoma (MB) is the most common malignant brain tumor in children. Heat shock proteins (HSPs) comprise a superfamily of proteins that serve as molecular chaperones and are overexpressed in a wide range of human cancers. The purpose of the present study was to investigate the expression of HSP27 (pSer82), HSP27 (pSer15), HSP40, HSP60, HSP70, HSP90-α, Akt, and phospho-Akt by multiplex bead array assay of MBs. The results of HSP and Akt expression were correlated with MB subtype; immunohistochemical expression of Ki-67 index, bcl-2, and p53; and patients' prognosis. Methods The authors retrospectively evaluated 25 children with MB who underwent surgery. Immunohistochemical analysis of Ki-67, p53, and bcl-2 expression was performed in all cases. By using multiplex bead array assay, a simultaneous detection of HSP27 (pSer82), HSP27 (pSer15), HSP40, HSP60, HSP70, HSP90-α, Akt, and phospho-Akt was performed. Results Medulloblastoma with extensive nodularity had significantly lower HSP27 (pSer15) expression (p = 0.039) but significantly higher HSP60 expression (p = 0.021) than classic MB. Large-cell MB had significantly higher HSP70 expression (p = 0.028) than classic MB. No significant difference was found between HSP27 (pSer82), HSP40, HSP90-α, Akt, or phospho-Akt expression and MB subtype. Large-cell MBs had significantly higher Ki-67 index compared with classic MBs (p = 0.033). When analyzing all MBs, there was a significant negative correlation between HSP27 (pSer15) and Ki-67 index (r = −0.475, p = 0.016); a significant positive correlation between HSP70 expression and Ki-67 index (r = 0.407, p = 0.043); and a significant positive correlation between HSP70 expression and bcl-2 index (r = 0.491, p = 0.023). Patients with large-cell MB had a worse survival than those with classic MB, but the difference did not reach statistical significance (p = 0.076). Conclusions A substantial expression of several HSPs in MB was observed. Given that HSPs represent an attractive strategy for anticancer therapy, further studies, involving larger series of patients, are obviously necessary to clarify the relationship of HSPs with tumor aggressiveness and prognosis.

scholarly journals Ki-67 as a prognostic marker according to breast cancer subtype and a predictor of recurrence time in primary breast cancer

2010 ◽  
Vol 1 (5) ◽  
pp. 747-754 ◽  
Author(s):  
REIKI NISHIMURA ◽  
TOMOFUMI OSAKO ◽  
YASUHIRO OKUMURA ◽  
MITSUHIRO HAYASHI ◽  
YASUO TOYOZUMI ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Marker ◽  
Primary Breast Cancer ◽  
Breast Cancer Subtype ◽  
Recurrence Time ◽  
Ki 67 ◽  
Cancer Subtype

scholarly journals Therapeutic Efficacies of Artemisia capillaris Extract Cream Formulation in Imiquimod-Induced Psoriasis Models

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Song Yi Lee ◽  
Suyeong Nam ◽  
Sungyun Kim ◽  
Ja Seong Koo ◽  
In Kee Hong ◽  
...  
Keyword(s):  
Skin Lesions ◽  
The Other ◽  
Epidermal Thickness ◽  
Artemisia Capillaris ◽  
Cream Formulation ◽  
Cumulative Score ◽  
Intracellular Adhesion Molecule ◽  
Psoriasis Therapy ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

A cream formulation containing Artemisia capillaris (AC) extract (ACE) was developed for psoriasis therapy. Although ACE can be dissolved in organic solvents, its topical application is restricted because of toxicities. Therefore, a cream formulation was developed for the convenient and safe local application of ACE on skin lesions. The antipsoriatic properties of the ACE cream were evaluated using an imiquimod- (IMQ-) induced psoriasis-like mouse model. In psoriasis-like mouse models, the cumulative score (redness, thickness, and scaling) of the IMQ + ACE cream group was significantly lower than those of the other groups on day 4 (p < 0.05). The results of the hematoxylin and eosin staining of skin tissues revealed that the epidermal thickness value of the IMQ + ACE cream group was significantly lower than those of the other experimental groups (p < 0.05). The expression level of intracellular adhesion molecule-1 (ICAM-1), which indicates the leukocyte infiltration into the skin and subsequent interactions with keratinocytes, was also lower in the IMQ + ACE cream group than in the IMQ group. These results indicate that ACE cream formulation could be used safely and conveniently for psoriasis treatment.

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