Evaluation of The Antioxidant Properties of Abid Rahim Date Palm (Phoenix dactylifera L.)fruit

2019 ◽  
Vol 2 (1) ◽  
pp. 157-163
Author(s):  
B.M Mofio ◽  
P.E. Ofure

Free radicals are implicated as a cause and consequence of diverse health pathologies including neuro-degenerative diseases, cardiovascular ailments, diabetes mellitus, cancer, nephropathies, inflammatory disorders, auto-immune diseases, idiosyncratic reactions etc. There is however a renewed interest in the study of plants for novel antioxidants. The present study evaluated the antioxidant properties of the ethanol extract of Date palm (Phoenix datylifera L.) fruit using 2,2 diphenyl-1-picrylhydrazyl (DPPH) assay and also conducted phyto-chemical analysis using standard protocols. The crude extract produced a reduced antioxidant effect compared to ascorbic acid. Specifically, at high test concentrations (0.50 and 0.25 mg/ml), the mean antioxidant activity of the extract was 65.7% and 55.2% respectively relative to 79.0% and 76.8% with ascorbic acid at the same concentration. The extract also induced an abysmally low antioxidant activity of less than 32% below 0.25 mg/ml. Phyto-chemical analysis revealed that the extract contained flavonoids, alkaloids, steroids, terpenoids and cardiac glycosides. Phoenix datylifera L. fruit could be a potential source for isolation of potent antioxidant principles..

2020 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
Lisa Giovanny ◽  
Faliha Arinda Lestari ◽  
Nurul Marfira ◽  
Laksmi Ambarsari ◽  
Siti Warnasih

Dates seeds are one of the byproducts of the date palm juice industry which has the potential for further processing. Dates seeds contain active compounds that are known to have ability to inhibit αglucosidase. This study aims to measure the activity of 70% ethanol extract and the fraction of date palm seeds with a mechanism in inhibiting αglucosidase. The measurement of inhibitory activity was carried out by extracting date palm seeds using 70% water and ethanol. The extraction product is then fractionated with n-hexane, ethyl acetate, n-butanol, and water. Then the total phenolic and flavonoid calculations were calculated for each extract and fraction. Measurement of α-glucosidase inhibition activity was measured based on reaction of substrate to enzyme by the addition of inhibitors. The results showed that the ethanolextract had the highest total phenolic and flavonoid, which were 76.86 mg GAE / g and 21.19 mg QE / g, respectively. n-hexane fraction have the best inhibitory activity seen from the IC50 value, which is 12.69 mg/L. The kinetics of inhibition of 70% ethanol extract of date palm seeds are mixed inhibition. Keywords: α-glucosidase, date seeds, flavonoids, kinetic inhibition, phenolics


2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.


2019 ◽  
Vol 8 (2) ◽  
pp. 179-184
Author(s):  
Siti Warnasih ◽  
◽  
Ade Heri Mulyati ◽  
Diana Widiastuti ◽  
Zuniar Subastian ◽  
...  

Red Sayer is one of the date varieties from the United Arab Emirates. This variety is one type of dates that is imported into Indonesia and used as processed date palm juice producing waste, namely date seeds. Date seeds can be made into some coffee that is rich in antioxidants and does not contain caffeine. The purpose of this study is to determine the chemical characteristics, antioxidant activity, total phenol, and caffeine contents in coffee of the Red Sayer variety date seeds. Coffee of date seeds is made into powder and then analyzed for its contents of water, ash, protein, fat, carbohydrates and total sugar, besides its antioxidant activity, total phenol, and caffeine levels. The results of the analysis obtained are, as follows: 4.42±0.01% water, 1.17±0.04% ash, 8.55±0.64% protein, 7.34±0.07% fat, 78.52±0.76% carbohydrate, 16.39±0.01% total sugar, 23.81±0.22 µg/mL antioxidant activity (IC50) and 340.65±1.53 mg GAE / 100g total phenol, while caffeine is not detected. Red Sayer date seed coffee, therefore, can be a source of carbohydrates and natural antioxidants that does not contain caffeine.


2009 ◽  
Vol 15 (6) ◽  
pp. 583-590 ◽  
Author(s):  
I. Ben Thabet ◽  
S. Besbes ◽  
M. Masmoudi ◽  
H. Attia ◽  
C. Deroanne ◽  
...  

This study is a contribution to valorise date palm sap (Phoenix dactylifera L.) by elaboration of high quality syrup. Sap was concentrated by evaporation and the obtained product was characterized by its physicochemical, rheological, thermal, sensory properties and by its antioxidant activity. Syrups from date palm sap have a good nutritional value marked by high amounts of sugars (58—75 g/100 g fresh matter basis), minerals (2.1—2.6 g/100 g fresh matter basis) and phenolics (147.61—224.55 mg of ferulic acid equivalents/kg fresh weight). Syrup also presents an antioxidant activity that appears related to total phenolic content. Rheological properties indicate that syrup preserves a Newtonian behavior from 10 °C to 55 °C well modeled by Arrhenius equation. Hedonic evaluation showed that consumers’ appreciation of date palm syrup was not significantly different to the most known sap syrup: maple syrup.


2018 ◽  
Vol 4 (2) ◽  
pp. 182-189
Author(s):  
Yusraini Dian Inayati Siregar ◽  
Tarso Rudiana ◽  
Windi Riyadi

Dates are a component of dates that have 6.10-11.47% of all dates. In some countries, dates are a major problem in the processing industry because the seeds currently only become waste. This research was conducted to determine the solvent that produces the largest yield using the soxhletation method and determine its antioxidant activity. Dates are disoxletasion with a variety of solvents n-hexane, diethyl ether, and ethanol. Also carried out the variation of extraction time for 1.5; 2; and 2.5 hours. Based on the results of the study showed that the results of the largest date seed extract were obtained using ethanol of 4.93% for 2.5 hours. Antioxidant activity of date palm seed extract (IC50) using DPPH ethanol extract method has IC50 of 2.27 ppm; n-hexane extract 8396.56 ppm; and diethyl ether extract 9826.88 ppm. Inhibition of date palm seed extract using the TBA method derived from ethanol extract of 98.22% at a concentration of 250 ppm. The results of the analysis using LC-MS showed that ethanol extract was thought to contain 13-hydroxyabscisic acid compound, 1-ethylidene-4-methylidene-2oxo-7-(propan-2-il)-tocahydro-1H-indent-5-il- 3-methylpent-2-enoate, 12-hydroxy-acid (8,10,14) -eicosatrienoic and 1-hydroxy-3- (pentadecanoiloxy) propan-2-il (5,8,11,14) -icosa-5, 8,11,14-tetraenoate. The results of the analysis using GC-MS on n-hexane extract showed 2,4-di-tert-butylphenol and 9-octadecenoic acid. Diethyl ether extract contains 9-octadecenoic acid and mono (2-ethylhexyl) benzocarboxylic acid.  


2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Mustapha Boukouada ◽  
Zineb Ghiaba ◽  
Nadhir Gourine ◽  
Isabelle Bombarda ◽  
Mokhtar Saidi ◽  
...  

The fatty acid composition of date seed oil from two different date palm ( Phoenix dactylifera L.) cultivars, locally known as Degla-Baïdha and Tafezouine, were investigated. GC analysis revealed the presence of five dominant fatty acids: oleic C18:1 (46.51; 39.15%), lauric C12:0 (22.1; 28.5%), myristic C14:0 (10.7; 11.4%), palmitic C16:0 (9.6; 8.7%) and linoleic C18:2 (6.9; 6.1%). The oils was characterised by a low content of tocopherols (0.53; 1.41 μg/g). The antioxidant activity of the oils was investigated using the DPPH•(1,1-di-phenyl-2-picryl-hydrazyl) scavenging assay. The oils had a weak bleaching effect on DPPH• free radicals. This study showed that the qualities of the tested oils are highly comparable with those of some commercial seed oils of other plants. Furthermore, a statistical analysis using the hierarchy ascendant classification method was conducted in order to highlight the similarities and/or the differences regarding the contents of the main fatty acids found in some common plants and in the five most famous cultivars of Phoenix dactylifera of south eastern Algeria (Tafezouine, Degla-Baïdha, Deglet-Nour, Ghars, Tamdjouhert).


Author(s):  
Habchi A. ◽  
Dekmouche M. ◽  
Hamia C. ◽  
Saidi M. ◽  
Yousfi M. ◽  
...  

This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).


2017 ◽  
Vol 8 (1) ◽  
pp. 63-71
Author(s):  
Yetri Elisya ◽  
Harpolia Cartika ◽  
Anindita Rizkiana

Palm date fruits (Phoenix dactylifera L) have been used for medical treatment, one of the medical purpose as antioxidant, which is the coumpound for free radical scavenging. In this study used palm juice makes with non sentrifuged method by grounds 200 grams of palm fruit with 20 ml of aquadest and store in room temperature. For date palm syrup used in this study were from certain brand distributed on the market. Antioxidant activity was testing by using DPPH method and determined by spectrophotometry Uv-Vis in wavelength 517nm, and Total Phenolic Content was testing by Folin Ciocalteu method and determined by spectrophotometry Uv-Vis in wavelength 774nm.11 sample of date palm syrup was sampling by purposive sampling methods and then got 2 sample to tested. Date Palm Juice were made by Non Cetrifuged methods. All sample were tested antioxidant activity using DPPH and Total Phenolic Content using Folin Ciocalteu. This study obtained that best operating time for all sample in antioxidant activity is 30 minutes with the highest point is Date Palm Juice 91,54%, Sample X for 86,93% and Sampel Y 89,92%. There was a linear correlation between with the result Total Phenolic Content for Date Palm Juice 9,25 mgGAE/g, Sample X 4,13 mgGAE/g and Sampel Y 4,28 mgGAE/g. Date Palm Juice and Date Palm Syrup could scavenge free radical DPPH in huge concentration and contain phenolic compound.


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