scholarly journals Identifikasi Komposisi Kimia dan Uji Aktivitas Antioksidan dari Biji Kurma (Phoenix dactylifera)

2018 ◽  
Vol 4 (2) ◽  
pp. 182-189
Author(s):  
Yusraini Dian Inayati Siregar ◽  
Tarso Rudiana ◽  
Windi Riyadi
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Date Palm ◽  
Octadecenoic Acid ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Seed Extract ◽  
Hexane Extract ◽  
Ether Extract ◽  
Diethyl Ether Extract

Dates are a component of dates that have 6.10-11.47% of all dates. In some countries, dates are a major problem in the processing industry because the seeds currently only become waste. This research was conducted to determine the solvent that produces the largest yield using the soxhletation method and determine its antioxidant activity. Dates are disoxletasion with a variety of solvents n-hexane, diethyl ether, and ethanol. Also carried out the variation of extraction time for 1.5; 2; and 2.5 hours. Based on the results of the study showed that the results of the largest date seed extract were obtained using ethanol of 4.93% for 2.5 hours. Antioxidant activity of date palm seed extract (IC50) using DPPH ethanol extract method has IC50 of 2.27 ppm; n-hexane extract 8396.56 ppm; and diethyl ether extract 9826.88 ppm. Inhibition of date palm seed extract using the TBA method derived from ethanol extract of 98.22% at a concentration of 250 ppm. The results of the analysis using LC-MS showed that ethanol extract was thought to contain 13-hydroxyabscisic acid compound, 1-ethylidene-4-methylidene-2oxo-7-(propan-2-il)-tocahydro-1H-indent-5-il- 3-methylpent-2-enoate, 12-hydroxy-acid (8,10,14) -eicosatrienoic and 1-hydroxy-3- (pentadecanoiloxy) propan-2-il (5,8,11,14) -icosa-5, 8,11,14-tetraenoate. The results of the analysis using GC-MS on n-hexane extract showed 2,4-di-tert-butylphenol and 9-octadecenoic acid. Diethyl ether extract contains 9-octadecenoic acid and mono (2-ethylhexyl) benzocarboxylic acid.  

scholarly journals Screening and characterization of potential bioactive compounds from Selaginella wightii

2020 ◽  
Author(s):  
Abheepsa Mishra
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Fungal Infections ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Dpph Method

A chemical investigation of the whole plant of Selaginella wightii was undertaken and the extracts of Selaginella wightii were screened for antioxidant and antimicrobial activities . The antioxidant activity by DPPH method was higher in ethanol extract and diethyl ether extract. In β-carotene-Linoleic acid assay, ethanol extract and diethyl ether extract showed good inhibition zone exhibiting significant antioxidant activity. The various extracts were then examined for antimicrobial activity by using the standard well-diffusion method. The diethyl ether extracts, ethanol, and aqueous extracts showed higher levels of antimicrobial activity than the other extracts. All the extracts also showed good anticandidal activity. The extracts were found to be rich in flavonoids and three compounds were identified. Our study indicates the potential use of Selaginella wightii extracts for treating antimicrobial or fungal infections.

scholarly journals Relative Toxicity of Leaf Extracts of Eucalyptus globulus and Centella asiatica against Mosquito Vectors Aedes aegypti and Anopheles stephensi

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Savitha Sekhar Nair ◽  
Vinaya Shetty ◽  
Nadikere Jaya Shetty
Keyword(s):  
Aedes Aegypti ◽  
Diethyl Ether ◽  
Eucalyptus Globulus ◽  
Anopheles Stephensi ◽  
Centella Asiatica ◽  
Hexane Extract ◽  
Ether Extract ◽  
Leaf Extracts ◽  
Diethyl Ether Extract ◽  
Third Instar Larvae

The larvicidal activity of different solvent leaf extracts (hexane, diethyl ether, dichloromethane, and methanol) of Eucalyptus globulus and Centella asiatica against two geographically different strains of Aedes aegypti and Anopheles stephensi was investigated. The extracts were tested against the late third instar larvae of Aedes aegypti and Anopheles stephensi, and larval mortality was observed after 24 hours of treatment. LC50 and LC90 were calculated. The LC50 values of hexane extract of Eucalyptus globulus against the late third instar larvae of the BSN and JPN strains of Aedes aegypti and the DLC and KNG strains of Anopheles stephensi were 225.2, 167.7, 118.8, and 192.8 ppm, while those of the hexane extract of Centella asiatica were 246.5, 268.7, 50.6, and 243.5 ppm, respectively. The LC50 values of diethyl ether extract of Centella asiatica were 339.6, 134.5, 241, and 14.7 ppm. The hexane extracts of both plants and the diethyl ether extract of C. asiatica presented the highest potential for the control of Aedes aegypti and Anopheles stephensi. The present findings also reveal the necessity of assaying multiple strains of a species to fully comprehend the larvicidal efficacy of a compound.

scholarly journals Preliminary Chemical, Total Polyphenol, Total Flavonoid Contents and Antioxidant Activity of Eucalyptus camaldulensis Dehnh.

2020 ◽  
Vol 32 (5) ◽  
pp. 1230-1234 ◽  
Author(s):  
Nguyen Van Thuy ◽  
Nguyen Minh Tien ◽  
Nguyen Ngoc Quy ◽  
Mai Huyng Cang ◽  
Pham Minh Quan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Aqueous Extract ◽  
Eucalyptus Camaldulensis ◽  
Ethanolic Extract ◽  
Total Flavonoid ◽  
Ether Extract ◽  
Total Polyphenol ◽  
Diethyl Ether Extract ◽  
Pharmacologically Active

This study aimed to identify phytochemicals and evaluate antioxidant activities of different extracts from Eucalyptus camaldulensis, an aromatic plant in the Myrtaceace family. Examined extracts in different solvents such as diethyl ether extract (DEE), the ethanolic extract (EE) and the aqueous extract (AE). Total polyphenol and total flavonoid contents were determined via aluminum chloride colorimetric method. Antioxidant activity was performed via ABTS and DPPH radical scavenging assays. The results showed that a wide variety of pharmacologically active compounds such as alkaloid, oil, flavonoid, triterpenoid, saponin, tannin and polyphenol were present in the leaves of Eucalyptus camaldulensis. The result of quantitative determination showed that total polyphenol content of the diethyl ether extract, ethanolic extract and aqueous extract achieved 73.47 ± 1.64, 306.48 ± 3.87 and 76.47 ± 1.64 mgGAE/g, respectively. Meanwhile, total flavonoid content was 41.74 ± 2.21, 45.98 ± 1.79 and 18.05 ± 0.81 mgQE/g, respectively. The ethanolic extract exhibited the highest DPPH (IC50 = 10.52 ± 0.14 μg/mL) and ABTS (IC50 = 9.86 ± 0.17 μg/mL). These results indicate that Eucalyptus camaldulensis leaves can be used in dietary applications with the potential to reduce oxidative stress.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

2018 ◽  
Vol 11 (1) ◽  
pp. 322
Author(s):  
Sathvika Chintalapani ◽  
Swathi M. S. ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ether Extract ◽  
Sesuvium Portulacastrum ◽  
Flavonoid Content ◽  
Diethyl Ether Extract ◽  
Phenolic And Flavonoid

  Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays.Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

2018 ◽  
Vol 11 (1) ◽  
pp. 322
Author(s):  
Sathvika Chintalapani ◽  
Swathi M. S. ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ether Extract ◽  
Sesuvium Portulacastrum ◽  
Flavonoid Content ◽  
Diethyl Ether Extract ◽  
Phenolic And Flavonoid

  Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays.Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

Evaluation of The Antioxidant Properties of Abid Rahim Date Palm (Phoenix dactylifera L.)fruit

2019 ◽  
Vol 2 (1) ◽  
pp. 157-163
Author(s):  
B.M Mofio ◽  
P.E. Ofure
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Chemical Analysis ◽  
Date Palm ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L ◽  
The Mean ◽  
Novel Antioxidants

Free radicals are implicated as a cause and consequence of diverse health pathologies including neuro-degenerative diseases, cardiovascular ailments, diabetes mellitus, cancer, nephropathies, inflammatory disorders, auto-immune diseases, idiosyncratic reactions etc. There is however a renewed interest in the study of plants for novel antioxidants. The present study evaluated the antioxidant properties of the ethanol extract of Date palm (Phoenix datylifera L.) fruit using 2,2 diphenyl-1-picrylhydrazyl (DPPH) assay and also conducted phyto-chemical analysis using standard protocols. The crude extract produced a reduced antioxidant effect compared to ascorbic acid. Specifically, at high test concentrations (0.50 and 0.25 mg/ml), the mean antioxidant activity of the extract was 65.7% and 55.2% respectively relative to 79.0% and 76.8% with ascorbic acid at the same concentration. The extract also induced an abysmally low antioxidant activity of less than 32% below 0.25 mg/ml. Phyto-chemical analysis revealed that the extract contained flavonoids, alkaloids, steroids, terpenoids and cardiac glycosides. Phoenix datylifera L. fruit could be a potential source for isolation of potent antioxidant principles..

scholarly journals Potency of Ethanol Extracts Palm Seeds (Phoenix dactylifera L.) as Antidiabetic with Inhibition Kinetics Parameter

2020 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
Lisa Giovanny ◽  
Faliha Arinda Lestari ◽  
Nurul Marfira ◽  
Laksmi Ambarsari ◽  
Siti Warnasih
Keyword(s):  
Inhibitory Activity ◽  
Date Palm ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Total Phenolic ◽  
Phoenix Dactylifera L ◽  
Palm Juice ◽  
Ethanol Extracts ◽  
Fraction Measurement ◽  
Phenolic And Flavonoid

Dates seeds are one of the byproducts of the date palm juice industry which has the potential for further processing. Dates seeds contain active compounds that are known to have ability to inhibit αglucosidase. This study aims to measure the activity of 70% ethanol extract and the fraction of date palm seeds with a mechanism in inhibiting αglucosidase. The measurement of inhibitory activity was carried out by extracting date palm seeds using 70% water and ethanol. The extraction product is then fractionated with n-hexane, ethyl acetate, n-butanol, and water. Then the total phenolic and flavonoid calculations were calculated for each extract and fraction. Measurement of α-glucosidase inhibition activity was measured based on reaction of substrate to enzyme by the addition of inhibitors. The results showed that the ethanolextract had the highest total phenolic and flavonoid, which were 76.86 mg GAE / g and 21.19 mg QE / g, respectively. n-hexane fraction have the best inhibitory activity seen from the IC50 value, which is 12.69 mg/L. The kinetics of inhibition of 70% ethanol extract of date palm seeds are mixed inhibition. Keywords: α-glucosidase, date seeds, flavonoids, kinetic inhibition, phenolics

scholarly journals Isolation and Elucidation Structure of 28-Hydroxy-3-Friedelanone of Nyamplung (Callophyllum inophyllum, Linn.) Leaves

2020 ◽  
Vol 9 (2) ◽  
pp. 117-125
Author(s):  
Triana Kusumaningsih ◽  
◽  
Muhammad Widyo Wartono ◽  
Nais Puji Wijanarti ◽  
◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Nonpolar Solvent ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Isolated Compound

The isolation triterpenoid from Nyamplung (Callophyllum inophyllum, Linn.) leaves has been conducted. The isolation was employed by maceration using ethanol as solvent and liquids extraction using ethyl acetate. Ethyl acetate extract was partitioned successively using nonpolar solvent with hexane, dichloromethane, and diethyl ether, respectively. The diethyl ether extract was purified by column chromatography. The isolated compound of fraction D1 was obtained as white solids crystal with yield of 0.0035%. The isolated compound was determined based on the FTIR, 1HNMR, 13CNMR, HSQC, and HMBC spectra. The isolated compound was identified as 28-hydroxy-3-friedelanone.

scholarly journals Preliminary Phytochemical Screening of wild edible fruits from Boda and Kolli hills

2017 ◽  
Vol 3 ◽  
Author(s):  
S.P. Anand ◽  
S. Deborah
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Ethanol Extract ◽  
Vital Role ◽  
Kolli Hills ◽  
Diethyl Ether Extract ◽  
Therapeutic Value ◽  
Phytochemical Compounds ◽  
Wild Edible Fruits ◽  
Phytochemical Constituent

<span>Medicinal plant plays a vital role for ailment of various chronic diseases due to the presence of phytochemical constituent. The preliminary phytochemical compounds was studied by using the ethanol, ethyl acetate, diethyl ether, chloroform and aqueous extracts of 15 wild edible fruits collected from <em>Boda </em>and <em>Kolli</em> Hills. The phytochemical compounds such as Phenol, tannins, saponin, alkaloid, flavonoids, steroids, </span><span lang="EN-IN">phlobotannins</span><span>, terpenoids, anthroquinones and cardiac glycosides were screened in 15 edible fruits by using standard methods. The diethyl ether extract, ethyl acetate and ethanol extract of the selected fruits<strong> </strong>shows the presence of phytochemicals compound. The highest occurrence shows the high therapeutic value possessing majority of phytochemical constituent of active compounds. In Further studies, the compound from these fruits were isolated and used for medicinal purposes</span>

scholarly journals Chemical Characteristics, Antioxidant Activity, Total Phenol, and Caffeine Contents in Coffee of Date Seeds (Phoenix dactylifera L.) of Red Sayer Variety

2019 ◽  
Vol 8 (2) ◽  
pp. 179-184
Author(s):  
Siti Warnasih ◽  
◽  
Ade Heri Mulyati ◽  
Diana Widiastuti ◽  
Zuniar Subastian ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
United Arab Emirates ◽  
Date Palm ◽  
Natural Antioxidants ◽  
Phoenix Dactylifera ◽  
Total Sugar ◽  
Total Phenol ◽  
Chemical Characteristics ◽  
Phoenix Dactylifera L ◽  
Palm Juice

Red Sayer is one of the date varieties from the United Arab Emirates. This variety is one type of dates that is imported into Indonesia and used as processed date palm juice producing waste, namely date seeds. Date seeds can be made into some coffee that is rich in antioxidants and does not contain caffeine. The purpose of this study is to determine the chemical characteristics, antioxidant activity, total phenol, and caffeine contents in coffee of the Red Sayer variety date seeds. Coffee of date seeds is made into powder and then analyzed for its contents of water, ash, protein, fat, carbohydrates and total sugar, besides its antioxidant activity, total phenol, and caffeine levels. The results of the analysis obtained are, as follows: 4.42±0.01% water, 1.17±0.04% ash, 8.55±0.64% protein, 7.34±0.07% fat, 78.52±0.76% carbohydrate, 16.39±0.01% total sugar, 23.81±0.22 µg/mL antioxidant activity (IC50) and 340.65±1.53 mg GAE / 100g total phenol, while caffeine is not detected. Red Sayer date seed coffee, therefore, can be a source of carbohydrates and natural antioxidants that does not contain caffeine.

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