scholarly journals Comparison of wild and domesticated hot peppers fruit: volatile emissions, pungency and protein profiles

2021 ◽  
Vol 35 (3) ◽  
pp. 305-327
Author(s):  
Diego Comparini ◽  
Cosimo Taiti ◽  
Matteo Lanza ◽  
Federico Vita ◽  
Camilla Pandolfi ◽  
...  
Keyword(s):  
Back Propagation ◽  
Pepper Plant ◽  
Protein Profiles ◽  
Origin And Evolution ◽  
Cultural Aspects ◽  
Flight Mass Spectrometry ◽  
Sds Page ◽  
Tree Classifier ◽  
Hot Peppers ◽  
Species Specific

Capsicum plant species are globally cultivated in warm and temperate regions, being important for agro-economic, biological and cultural aspects. While their worldwide spread and their ability of cross-pollination to easily hybridize play an important role in the formation of numerous species and varieties but also create confusion for their classification. For this reason, the categorization of species and varieties is complex and several methods have been used to evaluate pepper plant origin and evolution. Therefore, the objectives of this study were to compare a wild pepper (Capsicum chacoense) with other two domesticated cultivars belonging to different species such as Capsicum annuum and C. baccatum and draw conclusions about their origins using different approaches. For this purpose three methodologies have been used and compared: the comparison of their fruits volatile organic compounds (VOCs) emissions , their capsaicin and dihydrocapsaicin content and the leaves proteomic profiles. The VOCs analysis has been conducted by a time-of-flight mass spectrometry (ToF-MS) with an innovative approach to better identify all the compounds detected, in particular using two different ionization agents (H3O+ and NO+) to better identify all the compounds detected. The VOCs and pungency analyses were then used to build back propagation neural networks (BPNN) and a Random Tree classifier to conduct a multivariate analysis and evaluate the most species-specific volatiles. The outcomes appeared to be a most accurate approach with respect to the traditional varieties descriptors used for peppers discrimination. The BPNN led to the identification of several putative volatiles as good candidates for the recognition of these species or significant nodes in a decision learning tool. Finally, protein profiles have been obtained by SDS-PAGE analysis on the leaves to perform a fast proteomic comparison among the species. The protein profiles showed the C. baccatum and C. chacoense were more similar to the domesticated pepper C. annuum.

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

scholarly journals A Clostridium perfringens outbreak traced to temperature-abused beef stew, Norway, 2012

2013 ◽  
Vol 18 (9) ◽  
Author(s):  
E Wahl ◽  
S Rømma ◽  
P E Granum
Keyword(s):  
Abdominal Pain ◽  
Food Safety ◽  
Clostridium Perfringens ◽  
Temperature Control ◽  
Team Member ◽  
Cohort Analysis ◽  
Gastrointestinal Illness ◽  
Protein Profiles ◽  
Duration Of Symptoms ◽  
Sds Page

On 21 January 2012, the Norwegian Food Safety Authority was informed about gastrointestinal illness among 111 swimming club members, who were staying at a hotel in Trondheim. A hotel dinner on 20 January was their only common meal. Kitchen staff were interviewed, and food leftovers and kitchen environment were sampled. A case was defined as a swimming team member staying at the hotel from 20 to 22 January, who fell ill with diarrhoea, abdominal pain or nausea during this period. A total of 43 cases were identified, with median duration of symptoms of 35 hours. cpe-positive Clostridium perfringens (3.8 x 108 CFU), but not Bacillus cereus, was isolated from beef stew eaten by cases. cpe-negative C. perfringens was detected in a sample from the kitchen floor. SDS-PAGE showed indistinguishable protein profiles among C. perfringens cultures isolated from the beef stew, but slightly different profiles from the culture isolated from the kitchen floor. Cohort analysis showed that eating beef stew and rice was significantly associated with illness. No pathogens were detected in the rice. The temperature control of the stew, but not of the rice, was poor. Our results strongly indicate that cases were infected by Clostridium perfringens in beef stew that had inadequate temperature control during preparation.

scholarly journals Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 509 ◽  
Author(s):  
Roxana Djalali Farahani-Kofoet ◽  
Katja Witzel ◽  
Jan Graefe ◽  
Rita Grosch ◽  
Rita Zrenner
Keyword(s):  
Negative Impact ◽  
Fusarium Species ◽  
Asparagus Officinalis ◽  
Root Characteristics ◽  
Disease Symptoms ◽  
Flight Mass Spectrometry ◽  
Significant Negative Impact ◽  
Species Specific ◽  
Specific Impact

Soil-borne pathogens can have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum and F. redolens. In this study, their species-specific impact regarding disease severity and root morphological traits was analysed. Additionally, various isolates were characterised based on in vitro physiological activities and on protein extracts using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The response of two asparagus cultivars to the different Fusarium species was evaluated by inoculating experiments. Differences in aggressiveness were observed between Fusarium species and their isolates on roots, while no clear disease symptoms became visible in ferns eight weeks after inoculation. F. redolens isolates Fred1 and Fred2 were the most aggressive strains followed by the moderate aggressive F. proliferatum and the less and almost non-aggressive F. oxysporum isolates, based on the severity of disease symptoms. Fungal DNA in stem bases and a significant induction of pathogenesis-related gene expression was detectable in both asparagus cultivars. A significant negative impact of the pathogens on the root characteristics total root length, volume, and surface area was detected for each isolate tested, with Fred1 causing the strongest effects. No significant differences between the tested asparagus cultivars were observed.

scholarly journals  Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 259-263 ◽  
Author(s):  
A. Aksakal
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Visual Assessment ◽  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Sds Page ◽  
Molecular Masses ◽  
Whole Cell Protein ◽  
Salmonella Serovars

This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE.

scholarly journals Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA

2004 ◽  
Vol 64 (2) ◽  
pp. 317-326 ◽  
Author(s):  
J. A. de O. Rodrigues ◽  
J. F. Höfling ◽  
F. C. A. Tavares ◽  
K. M. R. Duarte ◽  
R. B. Gonçalves ◽  
...  
Keyword(s):  
Candida Species ◽  
Profile Analysis ◽  
Protein Profile ◽  
Negative Control ◽  
Yeast Cells ◽  
Sds Page ◽  
Differential Medium ◽  
Oral Yeasts ◽  
Species Specific ◽  
High Level

The purpose of this work was to evaluate biochemical and serological methods to characterize and identify Candida species from the oral cavity. The strains used were five Candida species previously identified: C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. tropicalis, and Kluyveromyces marxianus, as a negative control. The analyses were conducted through the SDS-PAGE associated with statistical analysis using software, chromogenic medium, and CHROMagar Candida (CA), as a differential medium for the isolation and presumptive identification of clinically important yeasts and an enzyme-linked immunoabsorbent assay (ELISA), using antisera produced against antigens from two C. albicans strains. This method enabled the screening of the three Candida species: C. albicans, C. tropicalis, and C. Krusei, with 100% of specificity. The ELISA using purified immunoglobulin G showed a high level of cross-reaction against protein extracts of Candida species. The SDS-PAGE method allowed the clustering of species-specific isolates using the Simple Matching coefficient, S SM = 1.0. The protein profile analysis by SDS-PAGE increases what is known about the taxonomic relationships among oral yeasts. This methodology showed good reproducibility and allows collection of useful information for numerical analysis on information relevant to clinical application, and epidemiological and systematical studies.

Arcobacter cibarius sp. nov., isolated from broiler carcasses

2005 ◽  
Vol 55 (2) ◽  
pp. 713-717 ◽  
Author(s):  
Kurt Houf ◽  
Stephen L. W. On ◽  
Tom Coenye ◽  
Jan Mast ◽  
Jan Van Hoof ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Pairwise Comparison ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Mean Values ◽  
Specific Pcr ◽  
Sds Page ◽  
Arcobacter Butzleri ◽  
Species Specific

Twenty Gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in Arcobacter species-specific PCR tests but that yielded an amplicon in an Arcobacter genus-specific PCR test were isolated from 13 unrelated broiler carcasses. Numerical analysis of the profiles obtained by SDS-PAGE of whole-cell proteins clustered all isolates in a single group distinct from the other Arcobacter species. DNA–DNA hybridization among four representative strains exhibited DNA binding values above 91 %. DNA–DNA hybridization with reference strains of the current four Arcobacter species revealed binding levels below 47 %. The G+C contents ranged between 26·8 and 27·3 mol%. Pairwise comparison of 16S rRNA gene sequences revealed the mean values for similarity to the type strain of Arcobacter cryaerophilus (97·5 %), Arcobacter butzleri (96·5 %), Arcobacter skirrowii (96·0 %) and Arcobacter nitrofigilis (95·0 %). The levels of similarity to Campylobacter and Helicobacter species were below 88 and 87 %, respectively. The isolates could be distinguished from other Arcobacter species by the following biochemical tests: catalase, oxidase and urease activities; reduction of nitrate; growth at 25 and 37 °C under aerobic conditions; growth on 2–4 % (w/v) NaCl media; and susceptibility to cephalothin. These data demonstrate that the 20 isolates represent a single novel Arcobacter species, for which the name Arcobacter cibarius sp. nov. is proposed, with LMG 21996T (=CCUG 48482T) as the type strain.

scholarly journals Autolysis of Lactobacillus helveticus and Propionibacterium freudenreichii in Swiss cheeses: first evidence by using species-specific lysis markers

1998 ◽  
Vol 65 (4) ◽  
pp. 609-620 ◽  
Author(s):  
FLORENCE VALENCE ◽  
ROMAIN RICHOUX ◽  
ANNE THIERRY ◽  
AIRI PALVA ◽  
SYLVIE LORTAL
Keyword(s):  
Coenzyme A ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Helveticus ◽  
Propionibacterium Freudenreichii ◽  
Swiss Cheese ◽  
Specific Lysis ◽  
Cold Room ◽  
Sds Page ◽  
Intracellular Proteins ◽  
Species Specific

Lactobacillus helveticus and Propionibacterium freudenreichii are essential starters in Swiss cheesemaking and the release of their intracellular enzymes through autolysis could significantly influence ripening. To provide evidence of this lysis, cheese made from microfiltered thermized milk inoculated with Lb. helveticus ITGLH77, Prop. freudenreichii ITGP23 and a commercial Streptococcus thermophilus was assayed. Starter viability was determined and lysis was monitored during ripening by protein analysis with SDS-PAGE of aqueous cheese extracts and by immunoblot detection of intracellular proteins: dipeptidase (PepD) for Lb. helveticus and methylmalonyl coenzyme A mutase for Prop. freudenreichii. We verified that the species specificity of these lysis markers was towards the cytoplasms of all the species currently used in Swiss cheese. Lb. helveticus exhibited an almost complete loss of viability (99·9%) from the beginning of ripening in the cold room; concomitantly PepD appeared in the cheese extracts and was detected until the end of ripening. Damaged Lb. helveticus cells were also visualized by scanning electron microscopy. In addition, free PepD was also successfully detected in commercial Swiss-related cheeses. All these results clearly demonstrated the autolysis of Lb. helveticus in Swiss cheese. Prop. freudenreichii ITGP23 grew during warm room ripening and no loss of viability was detected after maximal growth (109 cfu/g cheese). Free methylmalonyl-coenzyme A mutase was detected at the end of ripening during cold storage, when the cheese extracts were concentrated 20-fold, demonstrating that the autolysis of Prop. freudenreichii was tardy and limited.

Modulation of protein profiles inRhizobiumsp. under salt stress

1996 ◽  
Vol 42 (6) ◽  
pp. 617-620 ◽  
Author(s):  
Deepti Saxena ◽  
Sunil Khanna ◽  
Mohammed Amin
Keyword(s):  
Salt Stress ◽  
Molecular Mass ◽  
Stress Proteins ◽  
Protein Profiles ◽  
Sds Page ◽  
The Difference ◽  
Induced Changes ◽  
Rhizobium Sp

Salinity-induced changes in the protein profiles in Rhizobium sp. exhibited alterations in the expression of as many as 19 proteins, which either showed an enhanced rate of synthesis or a decline in the levels as compared with controls. All these proteins were predominantly of low molecular mass (below 40 kDa) except for one (52 kDa). Induction and repression of proteins in salt-grown cells and salt-shocked cells were qualitatively similar. However, the difference in the protein profiles was more marked in salt-grown cells as compared with salt-shocked cells.Key words: Rhizobium, SDS–PAGE, salt stress, proteins.

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