scholarly journals Impact of Moroccan Crocus sativus L. tepals, corms, and stigmas extract on growth and photosynthetic pigments in tomato seedling

2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Amine Khoulati ◽  
E. Saalaoui

An experiment was carried out in a greenhouse to study the effect of aqueous extracts of Crocus sativus L. by-products on tomato plants. Three concentrations of tepals and corms were used by fertigation: 1 g/L, 2 g/L, and 3 g/L. The aqueous extract of the stigmas was used as a foliar application at 0.6 g/L. The experiment was carried out in a completely randomized block with three repetitions for each concentration. The concentration of tepal extract at 3 g/L significantly (p≤0.05) increased the plants' height, the chlorophyll a, b content. The same results were observed for the foliar treatment with stigmas; however, there was no effect of tepal extract on the carotenoid content. On the other hand, the concentration 2 g/L of the corms extract had a positive impact (p≤0.05) in the chlorophyll b content while the concentration of 3 g/L increased the plant's height, the chlorophyll a (p≤0.05). Current results indicate that Crocus sativus by-products could improve certain physiological aspects of the recipient plants and new and natural biostimulants.

2016 ◽  
Vol 4 (4) ◽  
pp. 103-107
Author(s):  
Deepa Srivastava ◽  
K. Shukla

Photosynthetic effects of the leaves extracts from Ipomoea cairica on Partheniumhysterophorus L were studied. Ipomoea cairica dried leaf powder (20g) was dissolved in distilled water (200ml) for foliar application. The seedlings were maintained under natural condition in Insect free net house. The experiment was carried out to analyze the effects of Ipomoea cairica leaves extract by measuring chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll and carotenoid contents in Parthenium. The experimental plants were sprayed 20 days after emergence and control plants were maintained by a simultaneous spray of distilled water. Leaves of Partheniumhysterophorus were collected at 5 days interval following the foliar application of Ipomoea cairica leaves extract up to 15th day. Results showed that Chlorophyll (total chlorophyll, chlorophyll a and b) and carotenoid content was adversely affected as the duration of Ipomoea cairica leaves extract increased. This potential of Ipomoea cairica can be used as herbicide to control noxious weed Partheniumhysterophorous.


Author(s):  
V. P. Karpenko ◽  
I. I. Mostoviak ◽  
A. A. Datsenko ◽  
R. M. Prytuliak ◽  
O. I. Zabolotnyi

The article presents the results of a field experiment to study the effects of different rates of the microbial preparation Diazobacterin under different methods of using the plant growth regulator Radostim (seed treatment before sowing and spraying crops) on the content of chlorophyll a and b in buckwheat leaves of Yelena variety, their sum and carotenoids. Determination of chlorophyll a and b, their sum and carotenoids was performed in selected samples of buckwheat leaves in the field conditions, in the phases of stem branching and the beginning of flowering according to the method described by V.F. Gavrylenko and T.V. Zhyhalova using a spectrophotometer. The pigment concentration was calculated from Holm-Wettstein's equations for 100 % acetone. It was found that the content of photosynthetic pigments in buckwheat leaves is conditioned by the weather, rates and methods of application of the studied microbial preparations and phases of plant development. In particular, the analysis of chlorophyll and carotenoid content in the early flowering phase of buckwheat plants showed a significant increase compared to the phase of stem branching, which may be due to increased physiological and biochemical processes in plants, against the background of improving mineral nutrition, growth and plant development. Thus, with the multiple use of the microbial preparation Diazobacterin in the rates of 150, 175 and 200 ml and the stimulator of plant growth Radostim in the rate of 250 ml / t for seed treatment before sowing, the content of chlorophylls a and b, their sum and carotenoids with increasing application of Diazobacterin increased and the excess relative to control ranged within: 29–31 % for chlorophyll a; 10–11 % for chlorophyll b; 24–26 % for their sum and 23–24 % for carotenoids. At the same time, with a complex application for pre-sowing seed treatment of a mixture of the microbial preparation Diazobacterin in the rate of 200 ml per hectare of seeds with the stimulator of plant growth Radostym in the rate of 250 ml / t followed by spraying on this background crops the stimulator of plant growth Radostim in the rate of 50 ml / ha buckwheat leaves form the highest content of chlorophyll a in comparison with the control (40 %); chlorophyll b (15 %); the sum of chlorophyll a + b (33 %) and carotenoids (29 %). These data indicate the creation of more favorable conditions for physiological and biochemical processes in plants, including photosynthesis, with the direct positive effect of which, functionally active pigment complex of the leaf apparatus of buckwheat is formed.


Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1295
Author(s):  
Sana Saleem ◽  
Zuzana Bytešníková ◽  
Lukáš Richtera ◽  
Robert Pokluda

To ensure sustainable agricultural production and protection of crops from various biotic and abiotic stresses, while keeping in view environmental protection, by minimal usage of chemicals, the exploitation of beneficial microorganisms and modern nanotechnologies in the field of agriculture is of paramount importance. This study aimed to investigate the effects of Serendipita indica and guanidine-modified nanomaterial on the growth, and other selected parameters, of cabbage, as well as incidence of black spot disease. S. indica was applied in substrate and by seed inoculation. S. indica had a positive impact on the development of plants, and resulted in reduced black spot severity. The maximum plant height (119 mm) and number of leaves (8.3) were observed in S. indica-treated plants. Pigments were enhanced, i.e., chlorophyll a (0.79 mg/g), chlorophyll b (0.22 mg/g), and carotenoid content (0.79 mg/g), by substrate treatment. The highest antioxidant capacity (9.5 mM/L), chlorophyll a and b (1.8 and 0.6 mg/g), and carotenoid content (1.8 mg/L) were reported in S. indica seed treatment. S. indica treatment resulted in 59% and 41% disease incidence decrease in substrate and seed treatment, respectively. Guanidine-modified nanomaterial was seen to be effective in improving plant growth and reducing disease incidence; however, it did not perform better than S. indica. Application of nanoparticles resulted in enhanced normalized difference vegetation index and fluorescence by increasing chlorophyll a, b, and carotenoid content. Nitrogen content was the highest in plants treated with nanoparticles. However, the effect of the combined application of fungus and nanoparticles was similar to that of S. indica alone in substrate treatment, although negative impacts were reported in the biochemical parameters of cabbage. S. indica has great potential to enhance plant growth and manage Alternaria incidence in cabbage crops.


2018 ◽  
Vol 47 (1) ◽  
pp. 24-33
Author(s):  
Atul BHARGAVA ◽  
Francisco FUENTES ◽  
Sudhir SHUKLA ◽  
Shilpi SRIVASTAVA ◽  
Deepak OHRI

A two-year study was conducted to evaluate the foliage yield potential in 13 germplasm lines of Chenopodium album for 3 successive cuttings. Correlations among foliage yield and its contributing traits, along with path analysis was also worked out. Foliage yield was maximum for C. album IC 107297, followed by C. album H.P. and C. album amaranticolor. The genotype × year interaction was non-significant for all the traits except stem diameter and moisture content. Leaf size, plant height and stem diameter showed significant positive correlation with foliage yield both at phenotypic and genotypic levels in all the cuttings. Chlorophyll a and chlorophyll b showed positive association with carotenoid content and negative association with ascorbic acid in all the cuttings as well as on pooled basis. Significant negative association was observed between leaves/plant and foliage yield at genotypic level in all the cuttings (Ist cutting: -0.472*; IInd cutting: -0.414*; IIIrd cutting: -0.480*) as well as on pooled basis (-0.591**). Protein content negatively affected foliage yield in all the cuttings. Fibre content had high negative value of direct path for pooled data but positively influenced foliage yield indirectly via leaves/plant, stem diameter, chlorophyll a, chlorophyll b and protein content. Ascorbic acid positively affected yield in Ist cutting as well as on pooled basis. Leaf size had high positive direct effect and significant positive association with foliage yield that indicates a true relationship between these traits. Leaf size also indirectly affected foliage yield in a positive direction through majority of other traits. Thus, direct selection for leaf size should be exercised to bring about improvement in foliage yield in C. album.


2014 ◽  
Vol 6 (3) ◽  
pp. 331-334
Author(s):  
Sali Ali ALIU ◽  
Imer RUSINOVCI ◽  
Shukri FETAHU ◽  
Bekim GASHI

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.


2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Amine Khoulati ◽  
Sabir Ouahhoud ◽  
Samira Mamri ◽  
Mouhssine Meziane ◽  
Mohammed Choukri ◽  
...  

The valorization of Crocus sativus L. by-products has become important given its interesting content of bioactive molecules. In the present study, aqueous tepal extract (ATE) studied eggplant seedling’s growth and physiology under a plastic tunnel. ATE was foliage sprayed 3 times every 15 days, with various concentrations (1 mg/mL, 2 mg/mL, 3 mg/mL) in addition to a treatment containing 2 mg/mL of tepals and 0.6 mg/mL of stigmas (T+S). The concentration of 2 mg/L ATE significantly ( p ≤ 0.05 ) increased the plant’s height, the chlorophyll content, and decreased antioxidant activity and MDA (malondialdehyde). However, the concentration 3 mg/mL inhibited the plant growth; increased the content of ascorbic acid and polyphenol; and resulted in lipid peroxidation and antioxidant activities increases, indicating oxidative stress ( p ≤ 0.05 ). On the other hand, T+S significantly influenced some parameters analyzed. Our findings demonstrate that ATE can act as a biostimulant at 2 mg/mL to enhance eggplant growth in plastic tunnel production and used in plant stress situations.


2017 ◽  
Vol 1 (4) ◽  
pp. 18
Author(s):  
S. Manikandan ◽  
P. Thamizhiniyan

The present study was conducted to characterize the effect of organic and inorganic fertilizer on the phytochemical constituents of sun flower. The phytochemical constituent such as chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were analyzed. From the result it was observed that both the treatments had significantly improved the phytochemical constituents of sunflower. Among the treatments, organic fertilizer is found to be more efficient than inorganic fertilizer over control. The present work indicates towards the beneficial effect or organic and inorganic fertilizers for the improvement of sunflower.


2010 ◽  
Vol 56 (No. 6) ◽  
pp. 263-267 ◽  
Author(s):  
M. Jain ◽  
S. Tiwary ◽  
R. Gadre

Treatment of maize seedlings with different concentrations of sorbitol decreased the rate of germination substantially. Root and shoot length was also reduced by sorbitol treatment, however, decrease in root length was lower than shoot length. Incubation of leaf segments from maize seedlings grown in continuous light with sorbitol decreased the fresh weight and increased the dry weight in a concentration-dependent manner. Sorbitol treatment also reduced the total chlorophylls, chlorophyll a as well as chlorophyll b; the decrease in chlorophyll 'b' being more prominent than chlorophyll 'a', however, carotenoid content was declined marginally. Supply of sorbitol decreased the protein and RNA content; however, proline content and in vivo nitrate reductase activity (NRA) were increased. The results demonstrate an inhibitory effect of sorbitol-induced stress on overall growth in maize. Amongst the biochemical parameters analysed, chlorophyll, protein and RNA contents were declined, while proline content and nitrate reductase activity were enhanced with sorbitol treatment.


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