scholarly journals Bioefikasi Neurotoksin Ekstrak Campuran Daun Permot dan Daun Batang Sereh Bentuk Semprot terhadap Kecoa Jerman (Blattella germanica)

2021 ◽  
Vol 27 (2) ◽  
pp. 95-101
Author(s):  
Flora Rumiati ◽  
Rina Priastini Susilowati ◽  
Sancia Nathania Legenie Banuang

Kecoa merupakan salah satu hama yang menjadi pembawa penyakit. Untuk mengatasi populasi kecoa diperlukan adanya insektisida yang efektif, aman dan tidak bersifat resistensi. Bahan kimia aktif di dalam ekstrak daun permot (Passiflora foetida) yang dapat digunakan sebagai insektisida antara lain ermanin, vitexin, harmalin, dan harmin pada permot. Bahan kimia aktif tersebut bersifat neurotoksin. Selain itu, digunakan kandungan minyak atsiri yang terdapat pada daun dan batang sereh (Cymbopogon nardus). Tujuan penelitian adalah untuk mengetahui bioefikasi pemberian ekstrak daun permot dan daun batang sereh terhadap mortalitas kecoa Jerman (Blattella germanica). Penelitian ini menggunakan metode eksperimental. Sampling penelitian dihitung dengan rumus Federer. Kecoa Jerman ditempatkan pada 6 kandang sesuai kelompok perlakuan dengan 5 kali ulangan. Kelompok perlakuan adalah kontrol negatif (tanpa paparan), kontrol positif (disemprot dengan insektisida sintetik berbahan praletin 0,170%, d-alletrin 0,135 % dan sipermetrin 0,100 %), kelompok perlakuan campuran ekstrak daun permot dan daun batang sereh dengan dosis 0,05%, 0,10%, 0,20%, dan 0,40%. Pengamatan dilakukan setelah 10 menit pertama penyemprotan, dilanjutkan tiap jam selama 6 jam dan 24 jam. Kecoa dinyatakan mati jika badan terbalik dan tidak ada gerakan. Dari hasil penelitian ini diperoleh nilai LC50 sebesar 341 ppm, dan nilai LC90 sebesar 1175 ppm. Berdasarkan uji one way Anova diperoleh hasil adanya perbedaan bermakna antara kelompok kontrol dengan kelompok perlakuan insektisida berbahan campuran ekstrak daun permot dan daun batang sereh. Dapat disimpulkan bahwa campuran daun permot dan daun batang sereh memiliki efek neurotoksik yang mematikan pada kecoa Jerman dosis 1175 ppm.

2020 ◽  
Vol 3 (1) ◽  
pp. 100-105
Author(s):  
Delisma Simorangkir

Depression is a mood disorder that is characterized by profound and sustained sadness and sadness until the loss of life passion. Lemongrass which contains eugenol is thought to have the potential as an antidepressant that works by inhibiting the Mono Amine Oxidase enzyme. This study aims to determine the effectiveness of antidepressant citronella extract (Cymbopogon nardus (L.) Rendle) on male white mice. This study used 5 treatment groups namely CMC Na 0.5%, amitriptilyn, lemongrass extract dose 1.5 mg/kgBB, 15 mg/kgBB and 150 mg/kgBB. Antidepressant testing was determined based on immobility time using the forsed swim test method. Data analysis using statistics was tested for normality and continued with the One Way Anova method with a 95% confidence level. The results showed that the most effective treatment group as an antidepressant was amitriptilyn followed by lemongrass extract dose 150 mg/kgBB. The conclusion of this study is that lemongrass extract dose 150 mg/kgBB has the effectiveness as an antidepressant but is no more effective than the comparison of amitrip


2021 ◽  
Vol 5 (3) ◽  
pp. 191-197
Author(s):  
Lutfiah Fitriani ◽  
Maria Tuntun ◽  
Marhamah Marhamah

Escherichia coli adalah kuman oportunis yang banyak ditemukan di usus besar manusia sebagai flora normal. Serai wangi merupakan tanaman yang dapat dimanfaatkan sebagai obat tradisional karena memiliki kandungan senyawa yang bersifat antibakteri. Serai wangi diketahui memiliki kandungan alkaloid, terpenoid, saponin, flavonoid, dan tanin. Tujuan penelitian ini adalah Mengetahui daya hambat ekstrak serai wangi (Cymbopogon nardus (L.) Rendl terhadap pertumbuhan bakteri Escherichia coli ATCC 25922. Jenis penelitian adalah eksperimen dengan metode Difusi Kirby Bauer. Konsentrasi ekstrak serai wangi yang digunakan adalah 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, dan 100% dengan 3 kali pengulangan. Hasil penelitian menunjukan bahwa ekstrak serai wangi mampu menghambat pertumbuhan bakteri Escherichia coli ATCC 25922 pada konsentrasi 10%-100% dengan rerata zona hambat 6,80mm – 16,70 mm. Analisa data menggunakan uji One-way Anova dengan hasil nilai P=0,000 (P<0,05) menunjukan bahwa ekstrak berpengaruh terhadap pertumbuhan bakteri Escherichia coli ATCC 25922, dilanjutkan uji beda nyata terkecil dengan P<0,05 menunjukan adanya perbedaan yang nyata setiap konsentrasi. Pada konsentrasi 100% dengan konsentrasi 50%-10%, konsentrasi 90% dan 80% dengan konsentrasi 40%-10%, konsentrasi 70% dengan konsentrasi 30%-10%, konsentrasi 60% dan 50% dengan konsentrasi 20%-10%, dan konsentrasi 40% dengan konsentrasi 10%.


2018 ◽  
Vol 7 (2) ◽  
pp. 50 ◽  
Author(s):  
Rina Priastini Susilowati ◽  
Win Darmanto ◽  
Nanik Siti Aminah

It has been successfully found new herbal mosquito coils called “Morizena” made from Passiflora foetida leaf extract (40%), Chrysantemum cinerariaefolium flowers seed extract (40%), essential oils of  leaves-stems of Cymbopogon nardus (20%). The herbal mosquito coils are more effective to kill Aedes aegypti compared to commercial synthetic mosquito coils made from Transfluthrin. Treatment of exposure to herbal mosquito coils “Morizena” on Aedes aegypti for 8 hours/day with concentration 500 ppm (P1), 1000 ppm (P2), 2000 ppm (P3), 3000 ppm (P4), 4000 ppm (P5) and treatment of exposure to synthetic mosquito coils Transfluthrin 2500 ppm (K1) as a positive control, and without treatment of exposure to mosquito coils (K0) as a negative control. Experimental animals used were adult Ae. aegypti mosquito with 25 mosquitoes in each treatment. Experimental design used is one-way Anova test with linear regression to calculate its LC50 and LC90. The results of test to herbal mosquito coils “Morizena” given for 8 hours/day obtained mortality of Ae. aegypti by 92% to a concentration of 3000 ppm (P4) and 100% to a concentration of 4000 ppm (P5) and 100% for test to synthetic mosquito coils transfluthrin 2500 ppm (K1). Ae. aegypti LC50 and LC90 value for treatment of exposure to herbal mosquito coils “Morizena” are 999 ppm and 2977 ppm. Treatment of herbal mosquito coils “Morizena” with graded doses up to 4000 ppm and synthetic mosquito coils Transfluthrin 2500 ppm causing an increase in the enzyme acetylcholinesterase activity of Ae. aegypti. The conclusion is based on the Ae. aegypti  LC90 value is 2977 ppm, which means the effective dose of herbal mosquito coils “Morizena” to kill Ae. aegypti is 2977 ppm.


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Mahmudi Mahmudi ◽  
Hari Santoso ◽  
Saimul Laili

Natural insecticides made from lemongrass plants and zodia which are environmentally friendly insecticides and contribute to mosquito mortality. The purpose of this study was to determine the effect of lemongrass insecticides  (Cymbopogon nardus) and leaves of zodiac (Evodia suaveolens) on mosquito mortality (Aedes aegypti), and to determine the treatment of insecticides that were more effective against the mortality of mosquitoes. The research method used the experimental completely randomized design (CRD) with 4 treatments and 5 replications, namely treatment 1 control; treatment of 2 insecticides zodia; treatment of 3 lemongrass insecticides; and treatment of 4 combination lemongrass insecticides and zodia. The results showed that the control treatment did not effect, whereas in the treatment of zodia insecticides, lemongrass and combinations there were influences, presumably this happened because of difference in the chemical compounds of the 2 plants against mosquitoes. The results showed that mosquito mortality was controlled by 0%; mosquito mortality with zodia insecticide of 28%; mosquito mortality with lemongrass insecticide of 16.4%; and mosquito mortality with combination lemongrass insecticides and zodiac of 43.2%. The results of the One Way ANOVA test showed that there were significant differences and tended to experience increased mosquito mortality after being treated. Insecticides that are more effective in causing mosquito mortality are combination insecticides of lemongrass and zodia leaves.Keywords: Vegetable Insecticides, Lemongrass Plants, Zodia Plants, Mosquitoes.ABSTRAKInsektisida alami yang terbuat dari tanaman serai dan zodia merupakan insektisida alami yang ramah lingkungan dan berperan terhadap mortalitas nyamuk. Tujuan dari penelitian ini untuk mengetahui pengaruh insektisida serai (Cymbopogon nardus) dan daun zodia (Evodia suaveolens) terhadap mortalitas nyamuk (Aedes aegypti) dan untuk mengetahui perlakuan insektisida yang lebih efektif terhadap mortalitas nyamuk (Aedes aegypti). Metode penelitian menggunkan eksperimental Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu perlakuan 1 kontrol; perlakuan 2 insektisida zodia; perlakuan 3 insektisida serai; dan perlakuan 4 insektisida kombinasi serai dan zodia. Hasil penelitian menunjukkan perlakuan kontrol tidak ada pengaruh terhadap mortalitas nyamuk, sedangkan pada perlakuan insektisida zodia, serai serta kombinasi terdapat pengaruh, diduga hal tersebut terjadi karena adanya perbedaan kandungan senyawa kimia dari 2 tanaman tersebut terhadap nyamuk. Hasil penelitian menunjukkan mortalitas nyamuk perlakuan kontrol 0%; mortalitas nyamuk dengan insektisida zodia 28%; mortalitas nyamuk dengan insektisida serai 16,4%; dan mortalitas nyamuk dengan insektisida kombinasi serai dan zodia 43,2%. Hasil analisis uji One Way ANOVA menunjukkan terdapat perbedaan yang signifikan dan cenderung mengalami peningkatan mortalitas nyamuk setelah diberi perlakuan. Insektisida yang lebih efektif dalam mengakibatkan mortalitas pada nyamuk yaitu insektisida kombinasi serai dan daun zodia.Kata kunci: Insektisida Nabati, Tanaman Serai, Tanaman Zodia, Nyamuk


2021 ◽  
Vol 5 (1) ◽  
pp. 25
Author(s):  
David Mohamad Qadafi ◽  
Poedji Hastutiek ◽  
Lilik Maslachah ◽  
Endang Suprihati ◽  
Muhammad Hambal

Indonesia is one of the largest tropical countries in the world and various diseases can arise in the tropics which are caused by animals as vectors. An example of a vector that can carry diseases is a mosquito. Mosquitoes are insects that live side by side with humans buy act as vectors of disease. Mosquito Aedes aegypti is a type of mosquito that can carry the virus that causes Dengue Hemorrhagic Fever. This research was conducted to determine the effectiveness of Permot leaf ethanol extract (Passiflora foetida L.) as an alternative repellent against adult mosquitoes Aedes aegypti. This research was conducted from October to December 2020 and used Permot leaf ethanol extract consisting of 3 cream concentrations, namely 2.5%, 5%, 7.5%, negative control using cream without permot leaf ethanol extract and positive control using mosquito cream. The data of this study were tested using one way ANOVA to find the effectiveness rate and comparations of the each Permot leaf repellent extract. This study proven that the permot leaf ethanol extract is effective as a repellent for Aedes aegypti mosquitoes.


2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies


Author(s):  
Aline Krindges ◽  
Vanusca Dalosto Jahno ◽  
Fernando Morisso

Incorporation studies of particles in different substrates with herbal assets growing. The objective of this work was the preparation and characterization of micro/nanoparticles containing cymbopogon nardus essential oil; and the incorporation of them on bacterial cellulose. For the development of the membranes was used the static culture medium and for the preparation of micro/nanoparticles was used the nanoprecipitation methodology. The incorporation of micro/nanoparticles was performed on samples of bacterial cellulose in wet and dry form. For the characterization of micro/nanoparticles were carried out analysis of SEM, zeta potential and particle size. For the verification of the incorporation of particulate matter in cellulose, analyses were conducted of SEM and FTIR. The results showed that it is possible the production and incorporation of micro/nanoparticles containing essential oil in bacterial cellulose membranes in wet form with ethanol.


Author(s):  
Fabiana da Costa BARROS ◽  
Deivide de Brito FREITAS ◽  
Vitor Duque Estrada do NASCIMENTO ◽  
Everaldo ZONTA ◽  
Leonardo Duarte Batista da SILVA
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