scholarly journals PENGARUH EKSTRAK BIJI Ganitri (Elaeocarpus sphaericus Schum.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

2020 ◽  
Vol 2 (01) ◽  
pp. 28-37
Author(s):  
Ega Bramaseta ◽  
Tristi Indah Dwi Kurnia ◽  
Hasyim As'ari
Keyword(s):  
Escherichia Coli ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Growth Of Bacteria

This research was conducted in the laboratory of Biology University PGRI Banyuwangi to determine the effect of ganitri seeds extract (Elaeocarpus sphaericus Schum.) in inhibiting the growth of bacteria Escherichia coli and to determine the Minimum Inhibitory Concentration (MIC) of ganitri seeds extract (Elaeocarpus sphaericus Schum.). The method is using wells with 1% tetracycline positive control and negative control of sterile distilled water. Serial concentrations ganitri seed extract (Elaeocarpus sphaericus Schum.) is used by 10%, 20%, 30%, 40%, and 50%, and in the MIC test using serial concentrations of 1%, 2.5%, 5%, 7.5% and 10%. Based on the test results, the largest inhibition zone is shown by a concentration of 50% with an average diameter of 0,954 cm zone of inhibition and inhibition zone indicated smallest concentration of 10% with an average diameter of 0,215 cm inhibition zone. ANOVA test result value (F.Hit> F.Table) with F.Hit amounted to 33,512 and F.Tabel significance value of 2.85 and 0.000 (P <0.05), as the value of P <0.05 then it can be concluded that there is a concentration of ganitri seeds extract (Elaeocarpus sphaericus Schum.) on the growth of test bacteria. The results of Duncan test showed the ganitri seeds extract (Elaeocarpus sphaericus Schum.) at a concentration of 50% have significantly different inhibatory zone by treatment with 10%, 20%, 30%, 40%, positive control, and negative control. While MIC ganitri seed ethanol extract (Elaeocarpus sphaericus Schum.) Were still able to inhibit the growth of test bacteria at a concentration of 1% with an average diameter of 0.037 cm inhibition zone. The Minimum Inhibitory concentration (MIC) ganitri seed extract (Elaeocarpus sphaericus Schum.) shown by a concentration of 1%, where this concentration is still able to inhibit the growth of bacteria Escherichia coli.  Keyword: Ganitri seed extract, Bacteria Escherichia coli, Serial concentration, Minimum Inhibitory concentration (MIC), Inhibition zone.

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Sukun (Artocarpus altilis) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli

2020 ◽  
pp. 10-20
Author(s):  
Fuan Maharani Fiana ◽  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Ery Purwanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Artocarpus Altilis ◽  
Escherichia Coli Bacteria

Diarrhea is caused by bacterial infections, especially Staphylococcus aureus and Escherichia coli. The breadfruit leaves (Artocarpus altilis) are known contain flavonoid compounds, alkaloids, saponins, and tannins which can be used as antibacterial. This research was conducted to determine the ethanol extract of the breadfruit leaves against Staphylococcus aureus and Escherichia coli bacteria. The manufacture of the breadfruit leaf extract was using a maceration method with 96% ethanol solvent. The result of the tube test and the TLC test was positive, the breadfruit leaf extract containing flavonoids and tannins which have potential as antibacterial. The antibacterial test was carried out using the disc diffusion method (Kirby-Bauer test) against Staphylococcus aureus and Escherichia coli bacteria incubated for 1 x 24 hours. The extract concentrations tested were 10%, 15% and 20%. The positive control was using erythromycin antibiotics and the negative control was using aquades. The identification of the chemical content of plants is done by tube test and the TLC test. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Staphylococcus aureus bacteria, the average diameter of inhibitory zones respectively were 3.67, 3.50 and 2.67 mm with the positive control diameter of inhibition zone was 18.5 mm, the negative control diameter of inhibition zone was 0 mm. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Escherichia coli bacteria, the average diameter of inhibitory zones respectively were 5.33, 3.17 and 3.33 mm with the positive control inhibition zone diameter of 28.5 mm and the negative control of inhibition zone diameter of 0 mm. The activity of ethanol extract of the breadfruit leaves against the Staphylococcus aureus and Escherichia coli bacteria is included in the weak category.

scholarly journals UJI DAYA ANTIMIKROBA EKSTRAK ETANOL DAUN SIRIH MERAH (Piper Crocatum Ruiz & Pav.) TERHADAP PERTUMBUHAN Staphylococcus aureus ATCC 6538, Eschericia coli ATCC 11229 DAN Candida albicans ATCC 10231 SECARA IN VITRO

Biomedika ◽  
2011 ◽  
Vol 5 (1) ◽  
Author(s):  
Anika Candrasari ◽  
M. Amin Romas ◽  
Ovi Rizky Astuti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Betel Leaf

Red betel leaf contains flavonoids, alkaloids and essential oils that are suspected able to inhibit the growth of microbes. This study aims to analyze antimicrobial activity of ethanol extract of red betel leaf agains to Staphylococcus aureus, Eschericia coli and Candida albicans. Subjects were red betel leaf ethanol extract at concentration of 2.5%, 5%, 10%, 20%, 40%, 80% and 100%. As a positive control used amoxycilin, chloramphenicol and ketoconazole. The study showed that concentration 10 % - 100 % inhibit the growth of Staphylococcus aureus. In Escherichia coli all the data obtained, having an average which is not much different from the mean of the negative control. While on Candida albicans average diameter of inhibition zone of extract concentration 40% p = 0.197 concluded that statistically has significant antifungal power compared with ketokonazole.Keywords: Ethanol extract, Piper Ruiz & Pav crocatum, Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albican

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals Aktivitas Ekstrak Daun Bangle (zingiber purpureum roxb.) Terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa

2020 ◽  
Vol 2 (1) ◽  
pp. 31-38
Author(s):  
Fajrin Noviyanto ◽  
Siti Hodijah ◽  
Yusransyah Yusransyah
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibitory Concentration ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
High Morbidity ◽  
Leaf Extracts ◽  
Control Solution

The bacteria that cause infections that can lead to high morbidity and mortality, the bacterium Pseudomonas aeruginosa. Bangle has a pharmacological activity as antibacterial, laxative, pancreatic lipase inhibitor, and protect cells from damage caused by oxidative stress. The purpose of this study are: to know the chemical constituents present in the extract of leaves bangle (Zingiber purpureum Roxb.) Can be efficacious as an antibacterial and knowing Minimal Inhibitory concentration (MIC) of the extracts of leaves bangle against Pseudomonas aeruginosa. Tests on the leaf extracts for antibacterial activity against Pseudomonas aeruginosa bangle made by the method of Kirby Bauer and solvents used are DMSO. Test solution with a concentration of leaf extract bangle 200, 400, 600, 800 and 1,000 ppm, the positive control solution (ciprofoxacin) and the solution negative control (DMSO). The results showed that the chemical constituents present in the extract of leaves bangle (Zingiber purpureum Roxb.) Are flavonoids, saponins, tannins, alkaloids and steroids. Value Minimum Inhibitory Concentration (MIC) of ethanol extract of the leaf bangle S bacteria Pseudomonas aeruginosa is a concentration of 40 % with an average diameter of 5.44 mm inhibitory. MIC extract ethanol extract of leaf bangle belonging to the bacterial activity that is strong enough..

UJI AKTIVITAS EKSTRAK ETANOL 96% BIJI SRIKAYA (Annona squamosa L.) SEBAGAI ANTIDIARE YANG DISEBABKAN OLEH BAKTERI Shigella dysenteriae DENGAN METODE DIFUSI CAKRAM

2017 ◽  
Vol 1 (2) ◽  
pp. 181
Author(s):  
Leonov Rianto ◽  
Indri Astuti Handayani ◽  
Annisa Septiyani
Keyword(s):  
Statistical Tests ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Negative Control ◽  
Seed Extract ◽  
Shigella Dysenteriae ◽  
Annona Squamosa ◽  
Inhibitory Power ◽  
Growth Of Bacteria ◽  
Positive Controls

This research aims to find out how to extract ethanol 96% seed custard apples as Antidiarrhoeal that is caused by bacteria Shigella dysenteriae with different concentrations and compare with chloramphenicol. In addition, also to find out whether their respective inhibitory power of concentration of ethanol 96% seed extract custard apples have a difference in meaning. Research done by experimental methods, was carried out in the laboratory of pharmaceutical Academy IKIFA. Testing was done using the diffusion disc method. The concentration of ethanol 96% seed extract custard apples are used i.e. 15%, 30%, 45%, 60%, and 75%. As positive controls used chloramphenicol 30 µg/ml and NaCl 0.9% as the negative control. Each test is carried out as many as eight repetitions. The results showed an average diameter drag power which formed on the test solution with a concentration of 15%, 30%, 45%, 60% and 75% respectively of 6,887 mm, 11,49 mm, 8,144 mm, 7,694 mm, 7,150 mm, whereas for chloramphenicol for 40,31 mm. Based on the results of the statistical tests there are meaningful differences between concentrations showed a meaningful difference between the concentration of 15% and 30%, 15% and 60% and 15% and 75%. Conclusion of this research is at a concentration of 15%, 96% ethanol extract of seeds of custard apples have been able to inhibit the growth of bacteria, Shigella dysenteriae

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

UJI DAYA HAMBAT EKSTRAK ETANOL RIMPANG TEMU GIRING (CURCUMA HEY NEANA VAL.) TERHADAP PERTUMBUHAN ESCHERICHIA COLI SECARA IN VITRO

2017 ◽  
Vol 1 (1) ◽  
pp. 68
Author(s):  
Aditya Maulana Perdana Putra ◽  
Rustifah Rustifah ◽  
Muhammad Arsyad
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Volatile Oil ◽  
Negative Control ◽  
Diffusion Method ◽  
Minimum Inhibitory Concentrations ◽  
Escherchia Coli

Infection is a major problem that the world’s attention. Infectious diseases have caused the death of over 13 million people worldwide every year, particularly in the developing countries such as Indonesia. One of the species of bacteria that cause infections are Escherichia coli. Curcuma heyneana rhizome (Curcuma heyneana Val.) is a plant that is often used as a traditional medicine to antibacterial. This study was intended to determine the antibacterial inhibition activity, and minimum inhibitory concentrations (MIC) from the usage of an ethanol extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) for the growth of an Escherchia coli by in vitro.Curcuma heyneana rhizome (Curcuma heyneana Val.) was extracted using maseration method with solvent ethanol. Each extracts identified the active compounds group consisting of flavonoids, saponins, curcumin, volatile oil, and tannins. This study was an experimental laboratoric by using the diffusion method with discs blank with 10  treatment groups concentration which are 100%, 50%, 25%, 12,5%, 6,25%, 3,12%, 1,56%, 0,78%, positive control and negative control with three repetitions.The phytochemical screenings analysis showed curcuma heyneana rhizome (Curcuma heyneana Val.) of ethanol extract containing flavonoids, saponins, curcumin, volatile oil, and tannins. Ethanol extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) had an effectiveness in inhibiting the growth of an Escherchia coli by in vitro. Minimum inhibitory concentrations (MIC) from the usage of an ethanol extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) for the growth of an Escherchia coli by in vitro is 12,5% with average inhibition zone 9,77 mm. The results showed that the ethanol extract curcuma heyneana rhizome (Curcuma heyneana Val.) had an effectiveness in inhibiting the growth of an Escherchia coli, it showed inhibition zone diameter due to higher concentrations of the resistance is increasing. Analysis of the test data with Kruskal-Wallis (sig) = 0,001 which is smaller than α < 0,05. It means that there are significant differences in the average diameter of an each concentration of ethanol extract of curcuma heyneana rhizome in i

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

scholarly journals Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

2020 ◽  
Vol 3 (2) ◽  
pp. 36-40
Author(s):  
Viki Ayu Intan Permatasari ◽  
Mutia Hariani Nurjanah ◽  
Wimbuh Tri Widodo
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
Traditional Medicines ◽  
Alternative Ingredients ◽  
High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

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