INSECTICIDAL SUSCEPTIBILITY AND DETOXIFICATION ENZYME ACTIVITIES IN MUSCA DOMESTICA L. (DIPTERA: MUSCIDAE) OF FIELD POPULATIONS

2021 ◽  
Vol 1 (4) ◽  
pp. 428-435
Author(s):  
Mikhail A. Levchenko ◽  
◽  
Elena A. Silivanova ◽  
Polina A. Shumilova ◽  
Natalya A. Sennikova ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Enzyme Activities ◽  
First Generation ◽  
Laboratory Strain ◽  
Probit Analysis ◽  
Field Population ◽  
Resistance Ratio ◽  
Glutathione S Transferase ◽  
Detoxification Enzyme ◽  
Detoxifying Enzyme

This paper presents the results of the study of insecticidal susceptibility to five modern insecticides and the assessment of detoxifying enzyme activities in house flies Musca domestica L. of two field populations collected at livestock facilities in the Tyumen region. The objects of the study were larva and 3-5-days old adults of M. domestica of the laboratory strain and the first generation obtained from insects of the field populations. The lethal doses for 50% mortality of acetamiprid, fipronil, ivermectin, chlorfenapyr, and deltamethrin against adults M. domestica were calculated by the probit analysis method based on the results of the assessment of intestinal insecticidal activities of these substances by the feeding tests. The results of toxicological experiments showed that adults M. domestica of one field population were tolerant to ivermectin (the resistance ratio was 4,0) while adults of the second field population were tolerant to deltamethrin (the resistance ratio was 4,5). The field populations of M. domestica tested in this study differed in terms of detoxifying enzyme activities in adults. Carboxylesterase and glutathione-S-transferase activities were statistically significantly more in M. domestica adults of the population that was tolerant to deltamethrin than those in adults of the population tolerant to ivermectin. It needs to take into account differences (in insecticidal susceptibilities and in enzyme activities) between insect field populations inhabiting different farms when one selects pest control means against insects in livestock and poultry farms.

scholarly journals Insecticide susceptibility of house flies (Musca domestica) from a livestock farm in Tyumen region, Russia.

2019 ◽  
Vol 22 (2) ◽  
pp. 213-219
Author(s):  
M. A Levchenko ◽  
E. A. Silivanova ◽  
G. F. Balabanova ◽  
R. H. Bikinyaeva
Keyword(s):  
Musca Domestica ◽  
First Generation ◽  
Lethal Dose ◽  
Probit Analysis ◽  
House Fly ◽  
Field Population ◽  
Insecticide Susceptibility ◽  
House Flies ◽  
Livestock Farm ◽  
Median Lethal Dose

The susceptibility of the field populations of the house fly Musca domestica L. (Diptera:Muscidae) from a livestock farm of the Tyumen region, Russia, to six insecticides (deltamethrin, cypermethrin, thiamethoxam, permethrin, fipronil, chlorfenapyr) is reported. After a forced contact of adult flies of susceptible strains and first generation of field populations with the residues of insecticides at the bottom of glass cups, the median lethal dose of each insecticide was determined by probit analysis. According to the resistance ratio, the susceptibility of the evaluated field population to insecticides increased in the order: cypermethrin < deltamethrin < permethrin ≤ chlorfenapyr < thiamethoxam < fipronil.

Negative cross resistance of Spodoptera litura Fabricius population of tomato to newer molecule spinetoram

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 127-132
Author(s):  
M. Visnupriya ◽  
N. Muthukrishnan
Keyword(s):  
Spodoptera Litura ◽  
Laboratory Strain ◽  
Field Population ◽  
Resistance Ratio ◽  
Cross Resistance ◽  
In Vivo Toxicity

Field population of Spodoptera litura from tomato ( resistant to the majority of the conventional insecticide molecules) were subjected to the in vivo toxicity of spinetoram 12 SC to assess whether cross resistance exists or not. Untreated larvae of both field and laboratory strains showed no mortality during 48 hours of feeding. After 48 hours of feeding on spinetoram 12 SC treated leaves, LC50s of field larvae were 0.28, 0.93, 3.71 and 7.11 ppm for the 2nd, 3rd, 4th and 5th instars of S. litura respectively. However, in the laboratory strain these values were 1.12, 5.86, 36.72 and 91.55 ppm for 2nd, 3rd, 4th and 5th instars of S. litura respectively. Resistance ratio was 0.25, 0.16, 0.10 and 0.08 for the 2nd instar up to the 5th instar of S. litura.

scholarly journals BIOLOGICAL PARAMETERS AND ENZYME ACTIVITIES IN MUSCA DOMESTICA UNDER SELECTION WITH FIPRONIL

2021 ◽  
pp. 295-300
Author(s):  
Levchenko ◽  
Silivanova ◽  
Shumilova ◽  
Sennikova ◽  
Kinareikina
Keyword(s):  
Musca Domestica ◽  
Enzyme Activities ◽  
Laboratory Strain ◽  
Resistance Mechanisms ◽  
House Fly ◽  
Individual Development ◽  
Detoxification Enzymes ◽  
Biological Parameters ◽  
World Knowledge ◽  
Control Programs

Insect resistance to insecticides is one of the main issues of veterinary, medicine, and horticulture around the world. Knowledge of insecticidal resistance mechanisms is crucial for the development of insect control programs. The aim of the present study was to assess some biological parameters and enzyme activities in the house fly Musca domestica L. under selection with fipronil. The selection of M. domestica with fipronil was conducted by non-choice feeding when adults in each generation were fed with sugar that was pre-treated with insecticide solution. In even-numbered year generation, we evaluated the duration of individual development stages, the weight of individuals, fertility, and activity of the main detoxification enzymes (monooxygenases, esterases, and glutathione-S-transferases) in larvae and adults. The assessment of insect susceptibility to fipronil showed that larvae in the tenth generation of the fipronil-selected strain were more susceptible to fipronil than the individuals in the laboratory strain, and adults did not differ from the control as per this indicator. In the tenth generation of the fipronil-selected strain, we found that the duration of the development period from the egg stage to the emergence of adults lasted longer (by 18%) compared to the laboratory line. We noted that the activity of monooxygenases and glutathione-S-transferase in larvae and adults varied in certain generations of the fipronil-selected strain.

scholarly journals TACTICS OF MUSCA DOMESTICA CONTROL AT OBJECTS OF VETERINARY AND SANITARY SUPERVISION

2019 ◽  
pp. 308-312
Author(s):  
Levchenko ◽  
Silivanova
Keyword(s):  
Musca Domestica ◽  
First Generation ◽  
Natural Populations ◽  
Resistance Index ◽  
Laboratory Culture ◽  
Probit Analysis ◽  
Control Measures ◽  
Laboratory Population ◽  
Development Of Resistance ◽  
Metered Dose

Prolonged use of chemical insecticides at veterinary and sanitary inspection facilities often leads to a decrease in the efficiency of disinsection measures, which is associat-ed with the development of resistance in natural insect populations, including Musca domestica L., house flies. The purpose of the presented work was to study the level of sensitivity of M. domestica to modern insecticides in livestock and poultry farms of the Tyumen region. Under laboratory conditions, the activity of modern insecticides from the pyrethroid, neonicotinoids, phenylpyrazols, pyrroles, and avermectins groups against the first generation of adult M. domestica of natural populations was measured using metered-dose contact. Using probit analysis, pooled lethal doses (LD50) of insecticides for natural populations of flies and sensitive laboratory culture were calculated. Next, we determined the resistance index for each insecticide as the ratio of their LD50 values for the natural and laboratory population. It has been found that flies of natural populations were sensitive to tested insecticides to varying degrees. The highest resistance indicator (57) was recorded for the acetamids of the fly population from the livestock farm. A population of flies sensitive to fipronil was also found in the poultry farm with resistance index 1. Given the results of labora-tory studies, this farm was effectively disinsected against M. domestica using fipronil-containing preparation. The tactics of pest control measures, based on preliminary laboratory studies of the sensitivity of flies and the selection of chemical insecticidal agents against a specific natural population, will effectively control the number of Musca domestica on objects of veterinary sanitary inspection.

THE RESISTANCE OF MUSCA DOMESTICA L. FIELD POPULATIONS TO MODERN INSECTICIDES

2019 ◽  
Vol 1 (4) ◽  
pp. 407-412
Author(s):  
M.A. Levchenko ◽  
◽  
E.A. Silivanova ◽  
V.A. Plashkina ◽  
P.A. Shumilova ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Musca Domestica ◽  
First Generation ◽  
Laboratory Culture ◽  
Resistance Ratio ◽  
Livestock Farm ◽  
Poultry Facilities ◽  
Poultry Farms ◽  
Livestock Farms ◽  
Lethal Doses

To plan and improve the efficiency of disinsection measures at livestock and poultry facilities, the information on the susceptibility of houseflies to modern insecticides is needed. The goal of this study was to determine the susceptibility level to fipronil, acetamiprid, ivermectin, and chlorfenapyr in Musca domestica L. field populations inhabiting agricultural facilities. The study was conducted in one poultry and in two livestock farms. The susceptibility of M. domestica adults of the first generation to insecticides was assessed by the method of dosed contacting, after which the median lethal doses and the resistance ratio were calculated relative to the laboratory culture. According to the results, the fly population from a cattlebreeding farm, in which neonicotinoid fly baits were used for ten years, was highly resistant to acetamiprid (resistance ratio 57) and tolerant to ivermectin (resistance ratio 10). The fly population from poultry farms, in which neonicotinoids were used as baits against flies for four years, was tolerant to acetamiprid (resistance ratio 5). The fly population from a livestock farm, in which insecticides have not been used for more than five years, was susceptible to all insecticides tested. The obtained results and literature data confirmed that the rate of the development of insecticide resistance in field populations of M. domestica depends on the form and duration of the application of insecticide formulations.

scholarly journals Detoxification of host plant phenolic aglycones by the spruce budworm

2018 ◽  
Author(s):  
Dominic Donkor ◽  
Zahra Mirzahosseini ◽  
Jacquie Bede ◽  
Eric Bauce ◽  
Emma Despland
Keyword(s):  
Enzyme Activity ◽  
Host Plant ◽  
Choristoneura Fumiferana ◽  
Spruce Budworm ◽  
Glutathione S Transferase ◽  
Detoxification Enzyme ◽  
Counter Measures ◽  
Ph Conditions ◽  
Detoxifying Enzyme ◽  
Midgut Ph

AbstractThis study examines the post-ingestive fate of two host-plant derived small-molecule phenolics (the acetophenones piceol and pungenol) that have previously been shown to be toxic to the outbreaking forest pest, spruce budworm (Choristoneura fumiferana). We test first whether these compounds are transformed during passage through the midgut, and second whether the budworm upregulates activity of the detoxification enzyme glutathione-s-transferase (GST) in response to feeding on these compounds. Insects were reared on either foliage or artificial diet to the fourth instar, when they were transferred individually to one of two treatment diets, either control or phenolic-laced, for approximately 10 days, after which midguts were dissected out and used for Bradford soluble protein and GST enzyme activity analysis. Frass was collected and subjected to HPLC-DAD-MS. HPLC showed that the acetophenones do not autoxidize under midgut pH conditions, but that glucose- and glutathione-conjugates are present in the frass of insects fed the phenolic-laced diet. GST enzyme activity increases in insects fed the phenolic-laced diet, in both neutral pH and alkaline assays. These data show that the spruce budwom exhibits counter-adaptations to plant phenolics similar to those seen in angiosperm feeders, upregulating an important detoxifying enzyme (GST) and partially conjugating these acetophenones prior to elimination, but that these counter-measures are not totally effective at mitigating toxic effects of the ingested compounds in the context of our artifical-diet based laboratory experiment.

Therapeutic Potential of Biochanin-A Against Isoproterenol-Induced Myocardial Infarction in Rats

2020 ◽  
Vol 18 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Sangeethadevi Govindasami ◽  
Veera Venkata Sathibabu Uddandrao ◽  
Nivedha Raveendran ◽  
Vadivukkarasi Sasikumar
Keyword(s):  
Myocardial Infarction ◽  
Antioxidant Enzymes ◽  
Therapeutic Potential ◽  
Biochanin A ◽  
Glutathione S Transferase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Creatine Kinase Mb ◽  
Male Wistar Rats ◽  
Detoxifying Enzyme ◽  
Cardioprotective Effects

Background: This study determined the effect of Biochanin A (BCA) on isoproterenol (ISO) induced Myocardial Infarction (MI) in male Wistar rats. Methods: Animals (weighing 150-180 g) were divided into four groups, with six animals in each group and pretreated with BCA (10mg/kg Body Weight [BW]) and ɑ-tocopherol (60mg/kg BW) for 30 days; and ISO (20mg/kg BW) was administrated subcutaneously on the 31st and 32nd day. Results: ISO-induced MI rats demonstrated the significant elevation of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, lactate dehydrogenase, creatine kinase-MB and cardiac troponin; however, concomitant pretreatment with BCA protected the rats from cardiotoxicity caused by ISO. Activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase significantly reduced in the heart with ISO-induced MI. Pretreatment with BCA produced a marked reversal of these antioxidant enzymes related to MI-induced by ISO. Conclusion: In conclusion, this study suggested that BCA exerts cardioprotective effects through modulating lipid peroxidation, enhancing antioxidants, and detoxifying enzyme systems.

Current Status of Pyrethroids Resistance in Aedes aegypti (Culicidae: Diptera) in Lahore District, Pakistan: A Novel Mechanistic Insight

2021 ◽  
Author(s):  
Saira Nawaz ◽  
Hafiz Muhammad Tahir ◽  
Muhammad Asif Mahmood ◽  
Muhammad Summer ◽  
Shaukat Ali ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Enzyme Level ◽  
Laboratory Strain ◽  
Current Status ◽  
Glutathione S Transferase ◽  
Development Of Resistance ◽  
Biochemical Assays ◽  
Dengue Transmission ◽  
Lambda Cyhalothrin ◽  
Glutathione S Transferases

Abstract Aedes aegypti (Linnaeus, 1762) is a major vector responsible for dengue transmission. Insecticides are being used as the most effective tool to control vector populations in Lahore, Pakistan. Control of Ae. aegypti is threatened by the development of resistance against insecticides. The current status of insecticide resistance was evaluated against pyrethroids (deltamethrin, cypermethrin, and lambda-cyhalothrin) in different populations of Lahore (Model Town, Mishri Shah, Sadar Cantt, Walton, and Valencia). The susceptibility of the larval and adult populations was tested following the standard WHO guidelines. Moderate to high levels of resistance were found against pyrethroids in the larval (RR50: 3.6–27.2 and RR90: 5–90) and adult populations (percentage mortality &lt; 98%). Biochemical assays revealed a statistically significant increase in the enzyme level in all field populations compared to the laboratory strain. The value of esterase was one-fold higher, monooxygenase was 3.9- to 4.7-fold higher, and glutathione S-transferases was 1.9- to 2.6-fold higher in field populations compared to the laboratory strain. These results depict the presence of resistance against deltamethrin, cypermethrin, and lambda-cyhalothrin in field populations of Lahore mediated by metabolic enzymes i.e. esterases, monooxygenases, and glutathione S-transferase.

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