scholarly journals TACTICS OF MUSCA DOMESTICA CONTROL AT OBJECTS OF VETERINARY AND SANITARY SUPERVISION

2019 ◽  
pp. 308-312
Author(s):  
Levchenko ◽  
Silivanova
Keyword(s):  
Musca Domestica ◽  
First Generation ◽  
Natural Populations ◽  
Resistance Index ◽  
Laboratory Culture ◽  
Probit Analysis ◽  
Control Measures ◽  
Laboratory Population ◽  
Development Of Resistance ◽  
Metered Dose

Prolonged use of chemical insecticides at veterinary and sanitary inspection facilities often leads to a decrease in the efficiency of disinsection measures, which is associat-ed with the development of resistance in natural insect populations, including Musca domestica L., house flies. The purpose of the presented work was to study the level of sensitivity of M. domestica to modern insecticides in livestock and poultry farms of the Tyumen region. Under laboratory conditions, the activity of modern insecticides from the pyrethroid, neonicotinoids, phenylpyrazols, pyrroles, and avermectins groups against the first generation of adult M. domestica of natural populations was measured using metered-dose contact. Using probit analysis, pooled lethal doses (LD50) of insecticides for natural populations of flies and sensitive laboratory culture were calculated. Next, we determined the resistance index for each insecticide as the ratio of their LD50 values for the natural and laboratory population. It has been found that flies of natural populations were sensitive to tested insecticides to varying degrees. The highest resistance indicator (57) was recorded for the acetamids of the fly population from the livestock farm. A population of flies sensitive to fipronil was also found in the poultry farm with resistance index 1. Given the results of labora-tory studies, this farm was effectively disinsected against M. domestica using fipronil-containing preparation. The tactics of pest control measures, based on preliminary laboratory studies of the sensitivity of flies and the selection of chemical insecticidal agents against a specific natural population, will effectively control the number of Musca domestica on objects of veterinary sanitary inspection.

scholarly journals Insecticide susceptibility of house flies (Musca domestica) from a livestock farm in Tyumen region, Russia.

2019 ◽  
Vol 22 (2) ◽  
pp. 213-219
Author(s):  
M. A Levchenko ◽  
E. A. Silivanova ◽  
G. F. Balabanova ◽  
R. H. Bikinyaeva
Keyword(s):  
Musca Domestica ◽  
First Generation ◽  
Lethal Dose ◽  
Probit Analysis ◽  
House Fly ◽  
Field Population ◽  
Insecticide Susceptibility ◽  
House Flies ◽  
Livestock Farm ◽  
Median Lethal Dose

The susceptibility of the field populations of the house fly Musca domestica L. (Diptera:Muscidae) from a livestock farm of the Tyumen region, Russia, to six insecticides (deltamethrin, cypermethrin, thiamethoxam, permethrin, fipronil, chlorfenapyr) is reported. After a forced contact of adult flies of susceptible strains and first generation of field populations with the residues of insecticides at the bottom of glass cups, the median lethal dose of each insecticide was determined by probit analysis. According to the resistance ratio, the susceptibility of the evaluated field population to insecticides increased in the order: cypermethrin < deltamethrin < permethrin ≤ chlorfenapyr < thiamethoxam < fipronil.

THE RESISTANCE OF MUSCA DOMESTICA L. FIELD POPULATIONS TO MODERN INSECTICIDES

2019 ◽  
Vol 1 (4) ◽  
pp. 407-412
Author(s):  
M.A. Levchenko ◽  
◽  
E.A. Silivanova ◽  
V.A. Plashkina ◽  
P.A. Shumilova ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Musca Domestica ◽  
First Generation ◽  
Laboratory Culture ◽  
Resistance Ratio ◽  
Livestock Farm ◽  
Poultry Facilities ◽  
Poultry Farms ◽  
Livestock Farms ◽  
Lethal Doses

To plan and improve the efficiency of disinsection measures at livestock and poultry facilities, the information on the susceptibility of houseflies to modern insecticides is needed. The goal of this study was to determine the susceptibility level to fipronil, acetamiprid, ivermectin, and chlorfenapyr in Musca domestica L. field populations inhabiting agricultural facilities. The study was conducted in one poultry and in two livestock farms. The susceptibility of M. domestica adults of the first generation to insecticides was assessed by the method of dosed contacting, after which the median lethal doses and the resistance ratio were calculated relative to the laboratory culture. According to the results, the fly population from a cattlebreeding farm, in which neonicotinoid fly baits were used for ten years, was highly resistant to acetamiprid (resistance ratio 57) and tolerant to ivermectin (resistance ratio 10). The fly population from poultry farms, in which neonicotinoids were used as baits against flies for four years, was tolerant to acetamiprid (resistance ratio 5). The fly population from a livestock farm, in which insecticides have not been used for more than five years, was susceptible to all insecticides tested. The obtained results and literature data confirmed that the rate of the development of insecticide resistance in field populations of M. domestica depends on the form and duration of the application of insecticide formulations.

scholarly journals Resistance of House Fly, Musca domestica L. (Diptera: Muscidae), to Five Insecticides in Zhejiang Province, China: The Situation in 2017

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Jin-Na Wang ◽  
Juan Hou ◽  
Yu-Yan Wu ◽  
Song Guo ◽  
Qin-Mei Liu ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Probit Analysis ◽  
House Fly ◽  
Zhejiang Province ◽  
House Flies ◽  
Development Of Resistance ◽  
High Dependency ◽  
High Level ◽  
Very High ◽  
Lethal Doses

Objectives. High dependency on pesticides could cause selection pressure leading to the development of resistance. This study was conducted to assess the resistance of the house fly, Musca domestica, to five insecticides, namely, permethrin, deltamethrin, beta-cypermethrin, propoxur, and dichlorvos, in Zhejiang Province. Methods. Field strains of house flies were collected from the 12 administrative districts in Zhejiang Province in 2011, 2014, and 2017, respectively. Topical application method was adopted for the bioassays. The probit analysis was used to determine the median lethal doses with the 95% confidence interval, and then the resistance ratio (RR) was calculated. The insecticides resistance in different years and the correlations of the resistance between different insecticides were also analyzed. Results. The resistance of field strains house flies to insecticides in Zhejiang Province was relatively common, especially for permethrin, deltamethrin, and beta-cypermethrin. The reversion of the resistance to dichlorvos was found, and most of the field strains in Zhejiang Province became sensitive to dichlorvos in 2017. Propoxur was much easier to cause very high level of resistance; the Hangzhou strain had the highest RR value more than 1000 in 2014, and five field strains had the RR value more than 100 in 2017. Compared to 2011 and 2014, the resistance of the house flies to propoxur and deltamethrin increased significantly in 2017. The resistance of permethrin, deltamethrin, beta-cypermethrin, and propoxur was significantly correlated with each other, and the resistance of dichlorvos was significantly correlated with beta-cypermethrin. Conclusions. Our results suggested that resistance was existed in permethrin, deltamethrin, beta-cypermethrin, and propoxur in the house flies of Zhejiang Province, while the resistance reversion to dichlorvos was found.

scholarly journals Sanitary and epidemiological significance and resistance to insecticides of houseflies Musca domestica (Analytical Literature Review, 2000-2021)

2021 ◽  
Vol 104 (2) ◽  
pp. 72-86
Author(s):  
T. A. Davlianidze* ◽  
O. Yu. Eremina
Keyword(s):  
Insect Resistance ◽  
Musca Domestica ◽  
Natural Populations ◽  
Control Measures ◽  
House Flies ◽  
The Past ◽  
Intensive Use ◽  
The World ◽  
Epidemiological Significance ◽  
Active Substances

Insect resistance to insecticides has been and remains a serious problem affecting insect control worldwide. The fight against houseflies is an important part of pest control measures due to the ability of insects to carry pathogens of infectious diseases of humans and animals. But many of the chemicals that have been shown to be effective against them cease to work after just a few years of use. The intensive use of insecticides in world practice has led to the development of natural populations of houseflies that are resistant to all groups of insecticides used to control them. This species is one of the ten insect species that have developed resistance to the maximal amount of active substances. The review summarizes and analyzes the data of foreign and Russian authors on the insecticide resistance of houseflies (Musca domestica L.) over the past 20 years. Data on the resistance of house flies to both traditional insecticides and new chemicals are presented. The main mechanisms of insect resistance and the factors of its development are described. Schemes of rotation of insecticides in the controlling winged flies are given. The review analyzed 101 sources from 11 countries of the world.

INSECTICIDAL SUSCEPTIBILITY AND DETOXIFICATION ENZYME ACTIVITIES IN MUSCA DOMESTICA L. (DIPTERA: MUSCIDAE) OF FIELD POPULATIONS

2021 ◽  
Vol 1 (4) ◽  
pp. 428-435
Author(s):  
Mikhail A. Levchenko ◽  
◽  
Elena A. Silivanova ◽  
Polina A. Shumilova ◽  
Natalya A. Sennikova ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Enzyme Activities ◽  
First Generation ◽  
Laboratory Strain ◽  
Probit Analysis ◽  
Field Population ◽  
Resistance Ratio ◽  
Glutathione S Transferase ◽  
Detoxification Enzyme ◽  
Detoxifying Enzyme

This paper presents the results of the study of insecticidal susceptibility to five modern insecticides and the assessment of detoxifying enzyme activities in house flies Musca domestica L. of two field populations collected at livestock facilities in the Tyumen region. The objects of the study were larva and 3-5-days old adults of M. domestica of the laboratory strain and the first generation obtained from insects of the field populations. The lethal doses for 50% mortality of acetamiprid, fipronil, ivermectin, chlorfenapyr, and deltamethrin against adults M. domestica were calculated by the probit analysis method based on the results of the assessment of intestinal insecticidal activities of these substances by the feeding tests. The results of toxicological experiments showed that adults M. domestica of one field population were tolerant to ivermectin (the resistance ratio was 4,0) while adults of the second field population were tolerant to deltamethrin (the resistance ratio was 4,5). The field populations of M. domestica tested in this study differed in terms of detoxifying enzyme activities in adults. Carboxylesterase and glutathione-S-transferase activities were statistically significantly more in M. domestica adults of the population that was tolerant to deltamethrin than those in adults of the population tolerant to ivermectin. It needs to take into account differences (in insecticidal susceptibilities and in enzyme activities) between insect field populations inhabiting different farms when one selects pest control means against insects in livestock and poultry farms.

scholarly journals Field Efficacy of Steinernema sp. (Rhabditida: Steinernematidae) on the Colorado Potato Beetle Overwintering Generation

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1464
Author(s):  
Maja Čačija ◽  
Renata Bažok ◽  
Majda Kolenc ◽  
Tena Bujas ◽  
Zrinka Drmić ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
First Generation ◽  
Control Measures ◽  
Recommended Dose ◽  
Steinernema Feltiae ◽  
First Year ◽  
Potato Beetle ◽  
Field Efficacy ◽  
Second Year ◽  
Economic Pest

Colorado potato beetle (CPB) is an economic pest of potato that has developed resistance to all classes of chemical insecticides, thus requiring alternative control measures. As a potential solution, entomopathogenic nematodes (EPNs) have proven effective in suppressing this pest, but their efficacy against overwintering generations of CPB in Croatia has not been sufficiently researched. The aim of this two-year (2018–2019) field study was to determine the efficacy of Steinernema feltiae and Steinernema carpocapsae applied to overwintering CPB adults. EPNs were applied at three doses (7.5 mil./10 m2, 5.0 mil./10 m2 (the recommended dose) and 2.5 mil./10 m2) by watering the soil where the adults were overwintering. The first-year results were satisfactory for both EPNs: the efficacy of S. feltiae ranged from 79.03% to 100.00%, while the efficacy of S. carpocapsae ranged from 77.32% to 96.22%. In the second year, the highest efficacy (69.57%) was obtained using the recommended dose of S. feltiae. Although the results are not consistent across the two years of our study and suggest further research, they indicate that EPNs have great potential in controlling overwintering CPB generations to reduce first generation abundance and damage, and also to prevent the spread of new generations to surrounding potato growing areas.

Inbreeding effects among inbred and outbred laboratory colonies of Peromyscus maniculatus

1992 ◽  
Vol 70 (4) ◽  
pp. 820-824 ◽  
Author(s):  
David O. Ribble ◽  
John S. Millar
Keyword(s):  
Performance Measures ◽  
Reproductive Performance ◽  
First Generation ◽  
Peromyscus Maniculatus ◽  
Survival Rates ◽  
Natural Populations ◽  
Outbreeding Depression ◽  
Sibling Pairs ◽  
Laboratory Colonies ◽  
And Control

We examined the effects of sibling matings upon reproductive performance among inbred and outbred laboratory colonies of Peromyscus maniculatus. The inbred colony was founded by 12 females collected from one locality in Alberta and bred for 20 generations, with 35–45 pairs each generation. The outbred colony consisted of first-generation mice born of wild-caught females from diverse areas in Alberta. Consistent with theoretical expectations, there were no differences in reproductive performance between sibling and control (outbred) pairs within the inbred colony of mice. In contrast, sibling pairs had significantly fewer young per litter than control pairs within the outbred colony. Reproductive performance measures (proportion breeding, days from pairing to first litter, number of litters, and total number of offspring produced) were also significantly lower among sibling pairs from the outbred colony than among sibling pairs from the inbred colony. Lastly, we predicted that reproductive performance of the control pairs from the outbred colony would be less than that of control pairs from the inbred colony, due to outbreeding depression. Contrary to our predictions, average litter survival rates were greatest among the outbred colony control pairs. We suggest that the benefits of inbreeding or outbreeding extend broadly across the inbreeding–outbreeding continuum in natural populations of northern Peromyscus.

scholarly journals Comparative Study of the Effect of Solvents on the Efficacy of Neonicotinoid Insecticides Against Malaria Vector Populations Across Africa

2022 ◽  
Author(s):  
Magellan Tchouakui ◽  
Tatiane Assatse ◽  
Leon M. J. Mugenzi ◽  
Benjamin D. Menze ◽  
Daniel Nguiffo-Nguete ◽  
...  
Keyword(s):  
Democratic Republic Of Congo ◽  
Natural Populations ◽  
Resistance Mechanisms ◽  
Lower Mortality ◽  
Malaria Vectors ◽  
Cross Resistance ◽  
Metabolic Resistance ◽  
Resistance Development ◽  
Development Of Resistance ◽  
Bottle Test

Abstract Background New insecticides with a novel mode of action such as neonicotinoids have recently been recommended for public health by WHO. Resistance monitoring of such novel insecticides requires a robust protocol to monitor the development of resistance in natural populations. In this study, we comparatively used three different solvents to assess the susceptibility of malaria vectors to neonicotinoids across Africa.MethodsMosquitoes were collected from May to July 2021 from three agricultural settings in Cameroon (Njombe-Penja, Nkolondom, and Mangoum), the Democratic Republic of Congo (Ndjili-Brasserie), Ghana (Obuasi), and Uganda (Mayuge). Using the CDC bottle test, we compared the effect of three different solvents (ethanol, acetone, MERO) on the efficacy of neonicotinoids against Anopheles gambiae s.l. In addition, TaqMan assays were used to genotype key pyrethroid-resistant markers in An. gambiae and to evaluate potential cross-resistance between pyrethroids and clothianidin.ResultsLower mortality were observed when using absolute ethanol or acetone alone as solvent (11.4- 51.9% mortality in Nkolondom, 31.7- 48.2% in Mangoum, 34.6- 56.1% in Mayµge, 39.4- 45.6% in Obuasi, 83.7- 89.3% in Congo and 71.05- 95.9% in Njombe pendja) compared to acetone + MERO for which 100% mortality were observed for all the populations. Synergist assays (PBO, DEM and DEF) revealed a significant increase of mortality suggesting that metabolic resistance mechanisms are contributing to the reduced susceptibility. A negative association was observed between the L1014F-kdr mutation and clothianidin resistance with a greater frequency of homozygote resistant mosquitoes among the dead than among survivors (OR=0.5; P=0.02). However, the I114T-GSTe2 was in contrast significantly associated with a greater ability to survive clothianidin with a higher frequency of homozygote resistant among survivors than other genotypes (OR=2.10; P=0.013). ConclusionsThis study revealed a contrasted susceptibility pattern depending on the solvents with ethanol/acetone resulting to lower mortality, thus possibly overestimating resistance, whereas the MERO consistently showed a greater efficacy of neonicotinoids but it could prevent to detect early resistance development. Therefore, we recommend monitoring the susceptibility using both acetone alone and acetone+MERO (8-10µg/ml for clothianidin) to capture the accurate resistance profile of the mosquito populations.

scholarly journals Biocidal potential of Eugenia pyriformis essential oil in the control of Rhipicephalus (Boophilus) microplus in the free-living cycle

2019 ◽  
Vol 39 (11) ◽  
pp. 879-888
Author(s):  
Jaqueline P. Medeiros ◽  
Wanessa C. Bortollucci ◽  
Eloisa S. Silva ◽  
Herika L.M. Oliveira ◽  
Caio F.A.A. Campo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Immersion Test ◽  
Probit Analysis ◽  
Boophilus Microplus ◽  
Control Measures ◽  
Cattle Tick ◽  
Free Living ◽  
Adult Females ◽  
Southern Cattle Tick ◽  
Cattle Herds

ABSTRACT: Brazil has one of the largest commercial cattle herds in the world, which naturally coexist with an enormous number of parasitic species. Southern cattle tick, Rhipicephalus (Boophilus) microplus, is among these species, interfering with animal productivity and causing losses to the beef and dairy cattle sector. The use of chemical acaricides in the control of this mite has resulted in the emergence of resistant populations. In this sense, alternative control measures using plants as sources of botanical acaricides have shown to be effective. Eugenia pyriformis Cambess is a Brazilian plant with antioxidant and antimicrobial activity; however, there are no reports on its acaricidal activity in the literature. The present study aimed to evaluate the acaricidal and larvicidal potential of E. pyriformis leaf essential oil (EO) on southern cattle tick at different stages of the reproductive cycle. E. pyriformis leaves were collected and dried, and had their EO extracted by hydrodistillation (3h) using a modified Clevenger apparatus. Chemical analysis was performed by gas chromatography coupled with mass spectrometry (GC/MS), and 32 compounds belonging to the sesquiterpene class were identified: hydrocarbons (17.98%) and oxygenated forms (81.96%), with spathulenol (43.65%) and caryophyllene oxide (12.17%) as the most common. The EO was evaluated by the Adult Immersion Test at the concentrations (500.00 to 3.12mg/mL) in which the following parameters were measured: mortality of females (%), hatchability of eggs (%), and product efficiency (%). Larvae were assessed by the Larval Packet Test at concentrations ranging from 25.00 to 0.00004mg/mL. Lethal concentrations (LC) required for killing 50 and 99.9% of adult females and larvae were determined using Probit analysis. LC50 and LC99.9 of EO were 0.06 and 24.60mg/mL and 1,208.80 and 2,538mg/mL for larvae and adult females, respectively. Action of the EO in the free-living cycle of R. (B.) microplus larvae was another parameter assessed. To this end, the larvae were deposited in pots containing Brachiaria decumbens and, after migration to the leaf apex, a solution containing LC99.9 (24.60mg/mL) of the EO was sprayed. After 24h, 72.25% of the larvae had died, indicating stability of the EO when subjected to uncontrolled temperature and humidity conditions. The mechanism of action through which the EO killed the larvae and adult females was investigated by the Bioautographic Method, which showed inhibition of 3.15mg/mL of the EO on the acetylcholinesterase (AChE) enzyme. The results found in the present experiment indicate that E. pyriformis essential oil is an alternative in the control of southern cattle tick in the larval (parasitic) and free-living cycle (non-parasitic) stages under field conditions.

scholarly journals Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)

Insects ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 340
Author(s):  
Naima Bel Mokhtar ◽  
Amal Maurady ◽  
Mohammed Reda Britel ◽  
Mustapha El Bouhssini ◽  
Costas Batargias ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Natural Populations ◽  
Geographical Location ◽  
Wolbachia Infection ◽  
Hessian Fly ◽  
Mayetiola Destructor ◽  
Laboratory Population ◽  
Rrna Gene ◽  
16S Rdna Pcr ◽  
Hessian Flies

Mayetiola destructor (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat producing areas in Morocco. Using specific 16S rDNA PCR assay, Wolbachia infection was observed in 3% of the natural populations and 10% of the laboratory population. High throughput sequencing of V3-V4 region of the bacterial 16S rRNA gene revealed that the microbiota of adult Hessian flies was significantly influenced by their native regions. A total of 6 phyla, 10 classes and 79 genera were obtained from all the samples. Confirming the screening results, Wolbachia was identified as well in the natural Hessian flies. Phylogenetic analysis using the sequences obtained in this study indicated that there is one Wolbachia strain belonging to supergroup A. To our knowledge, this is the first report of Wolbachia in Hessian fly populations. The observed low abundance of Wolbachia most likely does not indicate induction of reproductive incompatibility. Yet, this infection may give a new insight into the use of Wolbachia for the fight against Hessian fly populations.

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