STUDY OF TOXICITY AND SAFETY OF LIPOPHILIC EXTRACTS OF THE WHITE SEA BROWN ALGAE – FUCUS VESICULOSUS AND LAMINARIA SACCHARINA ON THE DAPHNIA MAGNA STRAUS MODEL

2020 ◽  
pp. 49-55
Author(s):  
N. P. Podosinovikova ◽  
K. A. Krasnov ◽  
A. A. Bondarenko ◽  
M. L. Alexandrova ◽  
M. A. Zaytseva ◽  
...  
Keyword(s):  
Biological Activity ◽  
Acute Toxicity ◽  
Daphnia Magna ◽  
Brown Algae ◽  
White Sea ◽  
Aquatic Organisms ◽  
Reproductive Activity ◽  
The White Sea ◽  
Order Of Magnitude ◽  
Method Of Extraction

Assessment of the acute toxicity, safety and biological activity of lipophilic extracts of the White Sea brown algae“– S. latissima and F. vesiculosus– on the model of zoohydrobionts Daphnia magna Straus has been performed.Two methods of obtaining dry lipid concentrate of kelp and fucus were tested. It has been shown that the acute toxicity of the lipid extract of kelp does not depend on the method of its preparation and is about 200 mg/L. The acute toxicity of fucus extract depends on the method of preparation. It is about 100 mg/L in the «cold» method of extraction and increases by an order of magnitude in the «hot» method. To study the biological activity, samples of brown algae obtained by «cold» extraction were used.In chronic experiments, preparations of brown algae were used in a concentration of 8.0 mg/L, which was less than 0,1 LC50 of the acute toxicity of fucus and did not cause toxic effects.For 24 days, the preparations were introduced in contact with Daphnia twice a week. Subsequently, the control and experimental hydrobionts were kept under the same conditions until the death of the last individuals. It has been shown that the preparations of S. latissima and F. vesiculosus in the proposed concentration by 20-30% increased the lifespan of Daphnia in normal conditions and by 50-60% in extreme conditions, while stimulating the reproductive activity of aquatic organisms by 3-4 times. The results obtained suggest that the studied drugs have pronounced adaptogenic and cytoprotective effects.

scholarly journals COMPARATIVE STUDY OF POLYPHENOLS OF BROWN ALGAE OF THE BARENTS SEA AND THE WHITE SEA, AS WELL AS THE WATERS OF THE NORTH ATLANTIC

2020 ◽  
pp. 129-137
Author(s):  
Ekaterina Dmitriyevna Obluchinskaya ◽  
Lubov Viktorovna Zakharova
Keyword(s):  
Brown Algae ◽  
Barents Sea ◽  
White Sea ◽  
Raw Materials ◽  
Dry Mass ◽  
Biologically Active ◽  
The White Sea ◽  
Polyphenol Content ◽  
Norwegian Sea ◽  
The Barents Sea

The polyphenol content in the brown algae of the Barents, White Seas, as well as the water areas of the Northwest Atlantic (the Norwegian Sea, the Faxflow bay of the Atlantic Ocean) located in Russia, Norway, Greenland, and Iceland are compared. Algae of the following species were used for this study: Fucus vesiculosus, Fucus spiralis, Fucus serratus, Ascophyllum nodosum, Fucus evanescens. It was found that the most productive raw materials for the extraction of polyphenolic compounds are brown algae F. vesiculosus, growing in Zavalishin Bay of the Barents Sea (Russia): the highest polyphenol content (14.4%) in the summer of 2019 was noted here. Polyphenols detected in F. vesiculosus in the summer from the White Sea on about. Great burnt (13.3%) (Russia), as well as in the Norwegian Sea, Cape Sydspissen (11.6%) (Norway). The minimum content of polyphenols was found in F. spiralis (0.7% dry mass) on the coast of Iceland (Faxflow bay), a low content of polyphenols was characteristic of all types of algae from this location (0.7–2.4%). Three-way analysis of variance (MANOVA) on the example of three types of algae (F. vesiculosus, F. spiralis, A. nodosum) showed that all the studied factors (place of collection, type of algae, fertile phase) are significant. The most significant factor affecting the accumulation of polyphenols by brown algae is the location of algae growth. The high content of polyphenols in the types of algae we studied from Russian water areas allows us to recommend their use as food and medicinal raw materials, as well as raw materials for biologically active additives.

Toxic Metabolites of Micromycetes in Brown Algae of the Families Fucaceae and Laminariaceae from the White Sea

2021 ◽  
Vol 47 (1) ◽  
pp. 35-38
Author(s):  
A. A. Burkin ◽  
G. P. Kononenko ◽  
A. A. Georgiev ◽  
M. L. Georgieva
Keyword(s):  
Brown Algae ◽  
White Sea ◽  
The White Sea ◽  
Toxic Metabolites

scholarly journals Sensitivity of Zebrafish (Danio rerio) Embryos to Hospital Effluent Compared to Daphnia magna and Aliivibrio fischeri

2020 ◽  
pp. S681-S691
Author(s):  
M WITTLEROVÁ ◽  
G JÍROVÁ ◽  
A VLKOVÁ ◽  
K KEJLOVÁ ◽  
M MALÝ ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Sublethal Effects ◽  
Aquatic Organisms ◽  
Spinal Curvature ◽  
Treated Wastewater ◽  
Zebrafish Embryos ◽  
Fish Embryo ◽  
Additional Tool ◽  
Aliivibrio Fischeri

The Fish Embryo Acute Toxicity (FET) Test was adopted by the Organisation for Economic Co-operation and Development as OECD TG 236 in 2013. The test has been designed to determine acute toxicity of chemicals on embryonic stages of fish and proposed as an alternative method to the Fish Acute Toxicity Test performed according to OECD TG 203. In recent years fish embryos were used not only in the assessment of toxicity of chemicals but also for environmental and wastewater samples. In our study we investigated the acute toxicity of treated wastewater from seven hospitals in the Czech Republic. Our main purpose was to compare the suitability and sensitivity of zebrafish embryos with the sensitivity of two other aquatic organisms commonly used for wastewater testing – Daphnia magna and Aliivibrio fischeri. For the aim of this study, in addition to the lethal endpoints of the FET test, sublethal effects such as delayed heartbeat, lack of blood circulation, pericardial and yolk sac edema, spinal curvature and pigmentation failures were evaluated. The comparison of three species demonstrated that the sensitivity of zebrafish embryos is comparable or in some cases higher than the sensitivity of D. magna and A. fischeri. The inclusion of sublethal endpoints caused statistically significant increase of the FET test efficiency in the range of 1-12 %. Based on our results, the FET test, especially with the addition of sublethal effects evaluation, can be considered as a sufficiently sensitive and useful additional tool for ecotoxicity testing of the acute toxicity potential of hospital effluents.

scholarly journals A comparative study of the acute toxicity of the herbicide atrazine to cladocerans Daphnia magna, Ceriodaphnia silvestrii and Macrothrix flabelligera

2014 ◽  
Vol 26 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Raquel Aparecida Moreira ◽  
Adrislaine da Silva Mansano ◽  
Lidiane Cristina da Silva ◽  
Odete Rocha
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Native Species ◽  
Aquatic Organisms ◽  
Toxicity Tests ◽  
Toxic Substances ◽  
Tropical Freshwaters ◽  
Test Organisms ◽  
Herbicide Atrazine ◽  
Acute Toxicity Tests

AIM: In this study we compared the sensitivity of three species of Cladocera, Daphnia magna, Ceriodaphnia silvestrii and Macrothrix flabelligera, to the commercial product of the herbicide Atrazine, the Atrazine Atanor 50 SC® (500 g/L), widely used on crops in Brazil. METHODS: Acute toxicity tests were performed at the nominal atrazine concentrations 2.25, 4.5, 9.0, 18.0, 36 and 72 mg L-1, on C. silvestrii and M. flabelligera and at 2.25, 4.5, 9.0, 18.0, 36, 72 and 144 mg L-1 on D. magna. The range of concentrations tested was established in a series of preliminary tests. RESULTS: The toxicity tests showed that the two species naturally occurring in water bodies in Brazil were more susceptible than Daphnia magna. The effective concentrations of Atrazine Atanor 50 SC® (EC50- 48 h) to the species M. flabelligera, C. silvestrii and D. magna were 12.37 ± 2.67 mg L-1, 14.30 ± 1.55 mg L-1 and 50.41 ± 2.64 mg L-1, respectively. Furthermore, when EC50 observed here for M. flabelligera and C. silvestrii were compared with published values of EC50 or LC50 (mg L-1) for various aquatic organisms exposed to atrazine, it was seen that these two cladocerans were the most sensitive to the herbicide. CONCLUSIONS: Considering these results and the broad distribution of C. silvestrii and M. flabelligera in tropical and subtropical regions, it is concluded that these native species would be valuable test organisms in ecotoxicological tests, for the monitoring of toxic substances in tropical freshwaters.

scholarly journals Acute toxicity of carbofuran to selected species of aquatic and terrestrial organisms

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 103-108 ◽  
Author(s):  
R. Dobšíková
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Poecilia Reticulata ◽  
Aquatic Organisms ◽  
Sinapis Alba ◽  
Agricultural Practice ◽  
White Mustard ◽  
Water Flea ◽  
Raphidocelis Subcapitata ◽  
The World

Carbofuran is an anticholinesterase carbamate commonly used as an insecticide, nematicide and acaricide in agricultural practice throughout the world. The aim of the study was to investigate the acute toxicity of carbofuran to selected species of aquatic organisms (the guppy Poecilia reticulata Peters, the water flea Daphnia magna Straus and the green alga Raphidocelis subcapitata Korsikov), and to a terrestrial organism (a white mustard Sinapis alba Linné). Daphnia magna Straus was found to be the most sensitive organism.    

scholarly journals Assessment of accumulation of petroleum hydrocarbons by the hydrobionts of the south-eastern part of the Onega bay of the White sea

2019 ◽  
Vol 65 (4) ◽  
pp. 462-474 ◽  
Author(s):  
N. V. Neverova ◽  
T. Ya. Vorobyeva ◽  
A. V. Chupakov
Keyword(s):  
Oil Spill ◽  
Petroleum Hydrocarbons ◽  
White Sea ◽  
Negative Impact ◽  
Aquatic Organisms ◽  
Fuel Oil ◽  
The White Sea ◽  
Oil Sand ◽  
Heavy Petroleum ◽  
Onega Bay

In order to assess the current state of the ecosystem of the southeast of the Onega Bay of the White Sea affected by fuel oil spill in 2003, the accumulation of petroleum hydrocarbons was analyzed by the dominant species of aquatic organisms collected on the littoral of the most polluted coast in the areas of Purnem and Lyamts villages. In 2012, samples of aquatic organisms were taken in an area where all the species discussed in this work are represented on a small area: bivalved mollusks, attached molluscs, gastropods, polychaetes. In 2013 and 2018, samples of hydrobionts were additionally selected, in the three-kilometer strip of the coast on either sides of the givin point. In 2012 and 2013, high concentrations of HC in the tissues of bivalves were recorded. In 2018, the concentrations of hydrocarbons in the tissues of the studied hydrobionts were comparable to background values. A non-parametric test of Mann-Whitney showed a significant decrease in НС in mussel tissue from 2013 to 2018, at a significance level of 0.05. Taking into account the low levels of HC in the aqueous medium (less than 1 MPC of fishfarm) and in bottom sediments (from 0.34 to 9.03 mg/kg, the median of 1.41 mg/kg) in 2018, and is comparable with the background contents of hydrocarbons in tissues of aquatic organisms. We can conclude that after 15 years of the fuel oil spill, the condition of the Cape Deep ecosystem in terms of the content of hydrocarbons returns to the baseline state, continuing emissions of oil-sand lumps do not adversely affect the ecosystem. Based on the work done, it can also be concluded that ecotoxocological methods are priority in assessing the prolonged (or delayed) accidental impact of heavy petroleum products on aquatic ecosystems. The conclusion about the presence or absence of a negative impact on the aquatic ecosystem of hydrocarbons, based solely on the analysis of abiotic components, may not be sufficiently informative because it does not take into account the accumulative and deferred effects, especially manifested in the cold Arctic waters.

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