Discussion on High-Yield Cultivation and Assembly Technology of Selenium-Rich Rice and Rapeseed Rotation in Taoyuan County

Taoyuan County is a large grain and rapeseed production county. Taking advantage of the resource advantage of soil rich in selenium in Taoyuan County [1], it promotes high-yield cultivation and assembly technology of selenium-rich rice and rapeseed rotation in one-season rice area, optimizing the aggregate structure of the soil, improving the soil ecology and reducing the content of heavy metals in the soil [2], laying the foundation for the continuous increase in agricultural efficiency and farmers' income. Through rice and rapeseed rotation, the overwintering base of rice field borers are reduced, and the incidence of pests and diseases in the coming year is effectively reduced [3]. The implementation of supporting technologies for rice-rapeseed rotation cropping and the widespread promotion of high-quality varieties and planting techniques have improved the level of farmers’ planting. After one season of rice harvesting, most of the farmland is left unused. Using winter fallow fields to develop selenium-enriched rapeseed industry can actually increase the income of farmers.

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Selection of high-quality sperm with thousands of parallel channels

Lab on a Chip ◽

10.1039/d0lc01182g ◽

2021 ◽

Author(s):

Mohammad Simchi ◽

Jason Riordon ◽

Jae Bem You ◽

Yihe Wang ◽

Sa Xiao ◽

...

Keyword(s):

Clinical Practice ◽

High Selectivity ◽

High Yield ◽

Sperm Selection ◽

High Quality ◽

Parallel Channels ◽

Selection Of

A 3D-structured sperm selection device is presented that achieves both high selectivity and high yield via thousands of parallel channels. The device significantly outperforms the best clinical practice by selecting ∼100 000 of higher-quality sperm.

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A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts

Toxins ◽

10.3390/toxins13060420 ◽

2021 ◽

Vol 13 (6) ◽

pp. 420

Author(s):

Yi Ma ◽

Liu Cui ◽

Meng Wang ◽

Qiuli Sun ◽

Kaisheng Liu ◽

...

Keyword(s):

Large Scale ◽

Expression System ◽

High Yield ◽

Wild Type ◽

High Quality ◽

Efficient Protection ◽

Bacterial Ghosts ◽

Cell Envelopes ◽

Extracellular Structures ◽

First Time

Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-type protein E (EW) from phage ΦX174 and the screened mutant protein E (EM) in the Escherichia coli BL21(DE3) strain. The results show that the lysis efficiency mediated by protein EM was improved. The implementation of the pLysS plasmid allowed nearly 100% lysis efficiency, with a high initial cell density as high as OD600 = 2.0, which was higher compared to the commonly used BG preparation method. The results of Western blot analysis and immunofluorescence indicate that the expression level of protein EM was significantly higher than that of the non-pLysS plasmid. High-quality BGs were observed by SEM and TEM. To verify the applicability of this method in other bacteria, the T7 RNA polymerase expression system was successfully constructed in Salmonella enterica (S. Enterica, SE). A pET vector containing EM and pLysS were introduced to obtain high-quality SE ghosts which could provide efficient protection for humans and animals. This paper describes a novel and commonly used method to produce high-quality BGs on a large scale for the first time.

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Rational engineering strategies for achieving high-yield, high-quality and high-stability of natural product production in actinomycetes

Metabolic Engineering ◽

10.1016/j.ymben.2021.06.003 ◽

2021 ◽

Author(s):

Qing-Ting Bu ◽

Yue-Ping Li ◽

Huang Xie ◽

Ji-Feng Li ◽

Zhong-Yuan Lv ◽

...

Keyword(s):

Natural Product ◽

High Stability ◽

High Yield ◽

High Quality ◽

Rational Engineering

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High Microsatellite and SNP Genotyping Success Rates Established in a Large Number of Genomic DNA Samples Extracted From Mouth Swabs and Genotypes

Twin Research and Human Genetics ◽

10.1375/twin.9.4.501 ◽

2006 ◽

Vol 9 (4) ◽

pp. 501-506 ◽

Cited By ~ 14

Author(s):

Josine L. Min ◽

Nico Lakenberg ◽

Margreet Bakker-Verweij ◽

Eka Suchiman ◽

Dorret I. Boomsma ◽

...

Keyword(s):

Success Rate ◽

Genomic Dna ◽

Snp Genotyping ◽

High Yield ◽

Success Rates ◽

Nucleotide Polymorphism ◽

High Quality ◽

Single Nucleotide ◽

Dna Yield ◽

Yield And Quality

AbstractIn this article, we present the genomic DNA yield and the microsatellite and single nucleotide polymorphism (SNP) genotyping success rates of genomic DNA extracted from a large number of mouth swab samples. In total, the median yield and quality was determined in 714 individuals and the success rates in 378,480 genotypings of 915 individuals. The median yield of genomic DNA per mouth swab was 4.1 μg (range 0.1–42.2 μg) and was not reduced when mouth swabs were stored for at least 21 months prior to extraction. A maximum of 20 mouth swabs is collected per participant. Mouth swab samples showed in, respectively, 89% for 390 microsatellites and 99% for 24 SNPs a genotyping success rate higher than 75%. A very low success rate of genotyping (0%–10%) was obtained for 3.2% of the 915 mouth swab samples using microsatellite markers. Only 0.005% of the mouth swab samples showed a geno-typing success rate lower than 75% (range 58%–71%) using SNPs. Our results show that mouth swabs can be easily collected, stored by our conditions for months prior to DNA extraction and result in high yield and high-quality DNA appropriate for genotyping with high success rate including whole genome searches using microsatellites or SNPs.

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High-yield synthesis of CsPbBr3 nanoparticles: Diphenylphosphine as a reducing agent and its effect in Pb-seeding nucleation and growth

Nanotechnology ◽

10.1088/1361-6528/ac46d8 ◽

2021 ◽

Author(s):

Eder Antonio Castillo-Ruiz ◽

Diana Fabiola Garcia-Gutierrez ◽

Domingo Ixcóatl Garcia-Gutierrez

Keyword(s):

Nucleation And Growth ◽

Reducing Agent ◽

High Yield ◽

Reaction Yield ◽

High Quality ◽

Stabilizing Agents ◽

Nucleation Sites ◽

Nucleation Mechanisms ◽

Attractive Option ◽

Mass Increment

Abstract Based on the reported nucleation mechanisms for CsPbX3 and II-VI/IV-VI quantum dots, CsPbBr3 nanoparticles with a high reaction-yield, up to 393% mass-increment, were synthesized by the hot-injection method. The introduction of diphenylphosphine (DPP) as a reducing agent improved nanoparticle nucleation and growth, giving out evidence for Pb-seeding in CsPbBr3 nanoparticles formation. Additionally, a clear influence of the DPP in a CsPbBr3-Cs4PbBr6 incomplete phase transformation was observed, marked by the appearance of several PbBr2 nanoparticles, indicating the need for an improved ratio between the stabilizing agents and the precursors, due to the increased number of nucleation sites produced by the DPP. The resulting CsPbBr3 nanoparticles showed high quality, as they displayed 70%-90% photoluminescence quantum yield (PLQY), narrow size distribution with an average nanoparticle size of ~10 nm and the characteristic cubic morphology reported in previous works. This increment in CsPbBr3 nanoparticles’ reaction yield will contribute to making them a more attractive option for different optoelectronic applications.

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Productivity and Yield Quality of Intersubspecies Crossing of Japonica-Indica

Buletin Plasma Nutfah ◽

10.21082/blpn.v24n1.2018.p19-30 ◽

2018 ◽

Vol 24 (1) ◽

pp. 19

Author(s):

Sobrizal Sobrizal ◽

Carkum Carkum ◽

Wijaya M. Indriatama ◽

Aryanti Aryanti ◽

Ita Dwimahyani

Keyword(s):

Genetic Variability ◽

Amylose Content ◽

Rice Production ◽

High Yield ◽

Food Crops ◽

Milled Rice ◽

Rice Varieties ◽

Pests And Diseases ◽

Self Sufficiency ◽

Yield Quality

<p>In the middle of 1980s, rice self-sufficiency in Indonesia has been achieved, but the growth of rice production slowed down since the 1990s. Narrow genetic variability of released rice varieties contributed largely to the occurrence of leveling of potential rice yield over the past decades. To enlarge the genetic variability, an intersubspecies crossing of Koshihikari (japonica) and IR36 (indica) has been performed. Through this crossing, three high yielding and high yield quality promising lines of KI 37, KI 238, and KI 730 have been obtained. The objective of this study was to evaluate the superiorities of these lines through multi-location yield trials, pests, diseases, and grain qualities examinations. Examination methods used followed the release food crops variety procedure issued by the Indonesian Ministry of Agriculture. The result of examinations showed that the average yield of KI 730 was 7.47 t/ha, it was significantly higher than that of Ciherang (6,73 t/ha). KI 730 has a good grain quality, with translucent milled rice, a high percentage of milled rice (78.0%) and head rice (91.01%). The texture of its cooking rice was soft, sticky, with the amylose content of 20.41%. In addition, pests and diseases resistances of KI 730 were better than those of other lines tested. After evaluation by National Food Crops Release Variety Team, the KI 730 line was released as a national superior variety with the name of Tropiko. Tropiko should become an alternative variety to grow widely in order to increase national rice production and farmers income.</p>

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Barofractionation as an Innovative Method to Obtain Pectic Polysaccharides

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.899.599 ◽

2021 ◽

Vol 899 ◽

pp. 599-605

Author(s):

Dara Slobodova ◽

Raisa Gorshkova ◽

Stanislav Pankov

Keyword(s):

High Pressure ◽

Physicochemical Parameters ◽

New Method ◽

High Yield ◽

Dynamic Mode ◽

High Quality ◽

Pectic Polysaccharides ◽

Ph Values ◽

Pectin Polysaccharides ◽

Innovative Method

An innovative method has been developed and an installation has been created for obtaining pectin polysaccharides in a dynamic mode under the influence of high pressure. The process of degradation of protopectin at various pH values pH of the hydrolyzing agent was studied using the example of sunflower baskets. It has been established that the use of the new method makes it possible to obtain high-quality target products with a high yield in gentle conditions. The possibility of combining the stages of hydrolysis-extraction and fractionation has been demonstrated, which makes it possible to control the process of obtaining pectic polysaccharides in the direction of obtaining substances with specified physicochemical parameters.

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The Behaviour of Some Grape Hybrid Elite Varieties Obtained at SCDVV Blaj, in 2014-2015

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:12364 ◽

2016 ◽

Vol 73 (2) ◽

pp. 219

Author(s):

Anca Cristina Babes ◽

Maria Iliescu ◽

Liliana Lucia Tomoiaga

Keyword(s):

Clonal Selection ◽

Continuous Process ◽

Sugar Accumulation ◽

Research Station ◽

Good Resistance ◽

High Quality ◽

Pests And Diseases ◽

High Sugar

At Research Station for Viticulture and Enology Blaj (SCDVV Blaj), is a continuous process to improve and to obtain the new grapevine varieties, by sexual crossbreeding and clonal selection, varieties with high sugar accumulation and with equilibrate acidity, which could ensure high quality wines. During 2014-2015 period, the hybrid elite varieties: 5-26 and 6-110 were compared to the control Pink Traminer 60 Bl. and Feteasca Regala 21 Bl. As a study result, the elite varieties, 5-26 and 6-110, stood out by sugar/acidity balance, with good resistance to pests and diseases.

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Reduction of Fertilizer Use in South China—Impacts and Implications on Smallholder Rice Farmers

Sustainability ◽

10.3390/su12062240 ◽

2020 ◽

Vol 12 (6) ◽

pp. 2240 ◽

Cited By ~ 4

Author(s):

Helena Wehmeyer ◽

Annalyn H. de Guia ◽

Melanie Connor

Keyword(s):

Impact Analysis ◽

Management Practice ◽

Guangdong Province ◽

Ease Of Use ◽

Impact Factors ◽

Fertilizer Use ◽

Pests And Diseases ◽

Rice Farmers ◽

Agricultural Efficiency ◽

Sustainability Research

Despite the overuse of fertilizer in China, yields have stagnated while environmental pollution has risen. To increase sustainable production, the “Three Controls” Technology (3CT) was adopted in Guangdong Province as an agricultural best management practice for rice production. Its goal is to reduce the fertilizer use of farmers while decreasing the number of unproductive tillers and controlling pests and diseases. The objective of this study was to determine the farmers’ perception of 3CT focusing on three different impact factors: economic, social, and environmental. Using a digital survey questionnaire application, 142 farmers from six villages in Guangdong Province were interviewed to evaluate perceived changes in their farming and livelihood since adopting 3CT. Results showed that the farmers were highly satisfied with 3CT. They perceived positive livelihood changes and increased agronomic performance with reduced fertilizer use. Farmers who had adopted 3CT for the longest perceived significantly higher levels of change, more benefits, and improved agricultural efficiency. The study showed that 3CT is highly appreciated by farmers due to its effectiveness, ease of use, and compatibility. Our model highlighted the relevance of including social and environmental impact analysis for sustainability research in agriculture. Ultimately, 3CT has the potential of being implemented in other regions of China.

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