Cytotoxic, Antioxidant and Antimicrobial Activity of Polygonum Chinensis Linn

2012 ◽  
Vol 4 (4) ◽  
pp. 1569-1574
Author(s):  
Srividya A.R. ◽  
A Shalom ◽  
Chandrasekhar R ◽  
Vijayan P ◽  
V.J Vishnuvarthtan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
Dilution Method ◽  
Cytotoxicity Studies ◽  
Ic50 Value ◽  
The Family ◽  
Stem Extract ◽  
Serial Dilution Method ◽  
Antioxidant And Antimicrobial Activity

Polygonum chinensis linn belongs to the family Polygonaceae. The plant used in this study was shade dried and extracted with methanol by a cold maceration process. Qualitiave phytochemicals estimation showed the presence of the majority of compounds including terpinoids, flavonoids, tannins, saponins and glycosides. All the extracts showed moderate inhibition ranged from 12- 14 mm against both Gram-positive and Gram-negative strains and 00-21 mm against fungal trains.  In the two-fold serial dilution method, the minimum inhibitory concentration of the extract ranges between 250 – >1000 µg/ml.  The leaf extract of polygonum chinensis showed the potent activity with an IC50 value of 18.59 ± 0.356 µg/ml. In DPPH, the lipid peroxidation and hydrogen peroxide method, the leaf extract showed potent antioxidant activity. Root and stem extract showed moderate antioxidant activity. All the extracts showed moderate cytotoxicity to all the five cell lines Vero, A-549, L6, BRL, 3A and L-929 with CTC50 value ranging from 400-125 µg/ml. Short-term cytotoxicity studies were conducted for all the extracts against (Dalton Lymphocytes ascites) cells.  The CTC50 value was found to be 435 µg/ml. Thus, the extracts of P. Chinensis may possess cytotoxic, antioxidant and antimicrobial activity.   

scholarly journals Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. & Thomson

2021 ◽  
Vol 9 (1) ◽  
pp. 2-8
Author(s):  
Geetanjali Upadhyay ◽  
Lalit M. Tewari ◽  
Geeta Tewari ◽  
Neha Chopra ◽  
Naveen C. Pandey ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Tinospora Cordifolia ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
The Family ◽  
Stem Extract

Background: Medicinal plants are considered a rich source of ingredients, which can be used in drug development and synthesis. Tinospora cordifolia (Wild.) Hook.f. & Thomson, commonly known as guduchi, heart-leaved moonseed and giloya is a herbaceous vine of the family Menispermaceae, has several beneficial properties including antioxidant activity. Aim: The present study was carried out to analyze the antioxidant activity of leaf and stem extracts of Tinospora cordifolia by using DPPH (1,1-Diphenyl-2-picrylhydrazyl) and ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) free radical scavenging assays. Materials and Methods: Dried and powdered leaves and stem of T. cordifolia were extracted with methanol. Ascorbic acid was taken as standard. Total phenolic content was estimated by using Folin-ciocalteu's reagent while total flavonoid content by aluminium chloride reagent to find the correlation of polyphenols with antioxidant activity. ABTS assay of methanolic leaf and stem extracts showed the highest scavenging activity as compared to the DPPH assay. Results: Methanolic stem extract showed higher phenolic and flavonoid content along with antioxidant activity as compared to the methanolic leaf extract. Conclusion: The stem extract exhibited more antioxidant activity than the leaf extract with regards to the all parameters analyzed.

scholarly journals Pharmacognostical, Phytochemical, Antioxidant and Antimicrobial Activity of Costus woodsonii

2019 ◽  
pp. 53-59
Author(s):  
Mukesh S Sikarwar ◽  
Chew Khai Szeek ◽  
Neeraj Paliwal
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Concentration Test ◽  
Antioxidant And Antimicrobial Activity

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals BIOLOGICAL ACTIVITY OF ESSENTIAL OIL OF PITANGA (Eugenia uniflora L.) LEAVES

2019 ◽  
Vol 36 (1) ◽  
Author(s):  
Fernanda Sviech ◽  
Karlos Eduardo Pianoski ◽  
Roberta Letícia Kruger ◽  
Valesca Kotovicz ◽  
Eduarda M. Bainy Molardi Bainy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Antibacterial Activities ◽  
Alternative Source ◽  
Eugenia Uniflora ◽  
Natural Extracts ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activity

Natural extracts present antioxidant activity and can replace synthetic antioxidants. This study aimed to extract essential oil from Pitanga (Eugenia uniflora L.) leaves and evaluate its chemical composition, as well as antioxidant and antibacterial activities. The essential oil was obtained by hydrodistillation for 3 hours with a yield of 1.34 ± 0.18 %. The total antioxidant activity was evaluated by the DPPH and phosphomolybdenum methods. The oil presented an IC50 value of 100.96 µL mL‑1 and 1503.44 ± 29.48 mg α‑tocopherol mL-1 of oil. The major compounds identified by the GC-MS were germacrone (13.57%), spathulenol (7.49%), curzerene (5.31%) and α-cadinol (4.70%). The oil was also evaluated for its antimicrobial activity and was effective against Staphylococcus aureus. These results show that essential oil of pitanga leaves have good antioxidant and antimicrobial activity, being an alternative source of bioactive compounds.

scholarly journals Antioxidant, Antimicrobial and Cytotoxic Activities of Samanea saman (Jacq.) Merr.

1970 ◽  
Vol 3 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Afia Ferdous ◽  
Mohammad Zafar Imam ◽  
Tajnin Ahmed
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Brine Shrimp ◽  
Dpph Radical ◽  
Cytotoxic Potential ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Samanea Saman ◽  
Soluble Fractions

In the present investigation the n-hexane, carbon tetrachloride and choloroform soluble fractions of crude methanolic extract of Samanea saman bark were tested for antioxidant, antimicrobial and cytotoxic potential. Antioxidant activity of the extracts was evaluated by DPPH radical scavenging assay and total antioxidant activity test. Antimicrobial activity was tested using disc diffusion method against thirteen bacteria and three fungi and cytotoxicity was tested by brine shrimp lethality bioassay. Chloroform and hexane soluble fraction showed IC50 value of 12μg/ml and 14μg/ml respectively in scavenging DPPH radical while the reference Butylated hydroxytoluene showed an IC50 value of 10μg/ml. The carbon tetrachloride fraction showed the highest total antioxidant capacity. The carbon tetrachloride fraction was also found to possess mild to moderate microbial growth inhibitory capacity. In the brine shrimp lethality bioassay, the n-hexane, carbon tetrachloride, chloroform soluble fractions showed LC50 value of 14.94μg/ml, 0.831μg/ml and 3.288μg/ml respectively. The results suggest good antioxidant and cytotoxic potential of chloroform and hexane soluble fractions and antimicrobial activity of carbon tetrachloride fraction of Samanea saman bark extract. Key Words: Samanea saman; Leguminoseae; Cytotoxicity; Antimicrobial; Antioxidant; Total antioxidant capacity. DOI: 10.3329/sjps.v3i1.6792S. J. Pharm. Sci. 3(1): 11-17

scholarly journals In-vitro antimicrobial and antioxidant activity of Argyreia cuneata (Willd.) Ker Gawl. (Convolvulaceae)

2018 ◽  
Vol 8 (6) ◽  
pp. 22-27
Author(s):  
TR Prashith Kekuda ◽  
Nitish A. Bharadwaj ◽  
MB Sachin ◽  
BK Sahana ◽  
GS Priyanka
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Radical Scavenging ◽  
Flower Extract ◽  
Stem Extract ◽  
Poisoned Food Technique ◽  
Antimicrobial And Antioxidant Activity

Objectives: Argyreia cuneata (Willd.) Ker Gawl. belongs to the family Convolvulaceae. The present study was performed to screen the potential of crude extract of various parts of A. cuneata to exhibit antimicrobial activity. Methods: Extraction of shade dried and powdered leaf, stem and flower of A. cuneata was carried out by maceration technique. Antibacterial and antifungal activity of extracts was evaluated by Agar well diffusion and Poisoned food technique respectively. Antioxidant activity was determined by DPPH radical scavenging, ABTS radical scavenging and ferric reducing assays. Results:  All extracts were effective in inhibiting test bacteria and the susceptibility of bacteria to extracts was in the order: Bacillus cereus > Shigella flexneri > Escherichia coli > Salmonella typhimurium. Leaf extract and stem extract exhibited highest and least antibacterial activity, respectively. Extracts were effective in causing inhibition of seed-borne fungi viz. Aspergillus niger and Bipolaris sp to >50%. Leaf extract exhibited marked antifungal activity followed by flower extract and stem extract. All extracts were shown to exhibit concentration dependent scavenging and reducing activity. Antioxidant activity of extracts observed was in the order: leaf extract > flower extract > stem extract.  Conclusion: Among various parts of A. cuneata, leaf extract exhibited marked antimicrobial and antioxidant activity. The plant can be employed as an effective antimicrobial and antioxidant agent in suitable form. Further studies may be undertaken to recover phytochemicals from the plant and to investigate the antimicrobial and antioxidant activity of isolated components. Keywords: Argyreia cuneata, Maceration, Antimicrobial, Agar well diffusion, Poisoned food technique, Antioxidant

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF COCOA POD THAT ASSOCIATED IN MALTODEXTRIN IN VARIOUS CONCENTRATION

2018 ◽  
Vol 5 (2) ◽  
pp. 123
Author(s):  
Asriani Hasanuddin ◽  
Chairil Anwar ◽  
Marhawati Mappatoba ◽  
Hafsah Hafsah
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Natural Food ◽  
Skin Extract ◽  
E Coli ◽  
Dpph Method ◽  
Antioxidant And Antimicrobial Activity ◽  
Peel Extract

Cocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives.

scholarly journals Cytotoxic, antioxidant, and antimicrobial activities of Celery (Apium graveolens L.)

2021 ◽  
Vol 17 (1) ◽  
pp. 147-156
Author(s):  
Mohammed Saleh Al Aboody ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antihypertensive Agents ◽  
Apium Graveolens ◽  
Cell Protein ◽  
Dilution Method ◽  
Local Market ◽  
Antioxidant And Antimicrobial Activity

Celery (Apium graveolens Linn, Family: Apiaceae) is a common edible herb used as a spice in the traditional medicine of several nations since time immemorial. The whole plant is extensively used in cooking as soups and salads. A. graveolens has various pharmacological properties such as anticancer, anti-obesity, anti-hepatotoxic, and antihypertensive agents. Hence, it is of interest to document the in vitro cytotoxic, antioxidant, and antimicrobial activity of A. graveolens. The plants were collected in the local market, shade dried, and different parts of the plants were extracted with 70% ethanol using a cold maceration process. Antioxidant tests were performed based on the various radical scavenging methods. Antimicrobial activity and MIC were completed using the respective cup-plate and two-fold serial dilution method. In vitro cytotoxic studies were achieved by the MTT; Sulphorhodamine B assayed total cell protein content. DLA and ESC cells determined the short-term toxicity. The leaf extract exhibited significant antioxidant properties against NO, DPPH, ABTS, LPO, and HPO methods. Thus, potential inhibition against Gram-positive, Gram-negative, and fungal strains within the MIC ranges of 250-500 μg/ml was observed. All the extracts of the plant presented in the study revealed greater cytotoxicity effects against five respective cancer cell lines, L6, Vero, BRL 3A, A-549, L929, and L-929 with the ranging of 443-168.5 μg/ml. Thus, we show that A. graveolens possess a potential cytotoxic, antioxidant, and antimicrobial activity.

scholarly journals Screening of Antioxidant and Antimicrobial Activity of Micromeria fruticosa serpyllifolia Volatile Oils: A Comparative Study of Plants Collected from Different Regions of West Bank, Palestine

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Nihaya Salameh ◽  
Naser Shraim ◽  
Nidal Jaradat ◽  
Motasem El Masri ◽  
Lina Adwan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Plant Extracts ◽  
Dilution Method ◽  
Volatile Oils ◽  
Three Samples ◽  
Broth Microdilution Method ◽  
Micromeria Fruticosa

Background. The investigation of volatile oils used in traditional medicine is vital to enhance the quality of healthcare. This study is aimed at screening the antioxidant and antimicrobial properties of Micromeria fruticosa serpyllifolia volatile oils from three different regions in Palestine (north, middle, and south). Methods. Volatile oils of three samples of M. fruticosa serpyllifolia were extracted using the microwave-ultrasonic apparatus. The antioxidant activity of the volatile oils was assessed by inhibition of DPPH free radical. The antimicrobial activity was examined using the broth microdilution method. Assessment of antifungal activity was achieved using the agar dilution method. Results. Screening the biological activity of plant extracts revealed that the sample from Ramallah (middle region) possessed the most potent antioxidant activity with an IC50 value of 0.45 μg/mL. The three samples exhibited broad antimicrobial activity and showed potential antifungal activity. The sample from the southern region showed the highest potency against Shigella sonnei with the lowest reported MIC; the sample from the northern region demonstrated the least potency against clinical isolate of Staphylococcus aureus and “methicillin”-resistant Staphylococcus aureus. Conclusions. The study showed that Micromeria fruticosa serpyllifolia volatile oil samples from different regions in Palestine possess different potential antioxidant and antimicrobial activities that were in line with traditional uses of the plant extracts.

scholarly journals FORMULATION OF SERUM GEL CONTAINING ANGELICA KEISKEI LEAF EXTRACT AS AN ANTIOXIDANT AND TYROSINASE ENZYME INHIBITOR

2020 ◽  
pp. 108-111
Author(s):  
DIAH LIA AULIFA ◽  
RIZKA Y. NOERFITRI ◽  
DEBY TRISTIYANTI ◽  
ARIF BUDIMAN
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Enzyme Inhibitor ◽  
Physical Stability ◽  
Ic50 Value ◽  
Angelica Keiskei ◽  
Inhibitory Activities ◽  
Tyrosinase Enzyme ◽  
Inhibitory Power ◽  
Skin Lightening

Objective: The aim of this study was to formulate and evaluate the efficacy of Angelica keiskei (AK) leaf extract in serum gel as an antioxidant and tyrosinase enzyme inhibitor. Methods: The leaves of AK were extracted using the maceration method, while the antioxidant and tyrosinase enzyme inhibitory activities were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and L-Tyrosine as assay and substrate, respectively. Subsequently, the extract was formulated into serum gel and evaluated for physical stability, antioxidant and inhibitory activity to tyrosinase enzyme. Results: The results showed that AK leaf extract has antioxidant activity, with an IC50 value = 7.73 mg/ml, while the tyrosinase enzyme inhibitory power had an IC50 value = 500 mg/ml. Based on physical stability, the serum gel was stable after 28 d of storage. The serum gel of AK leaf extract also showed antioxidant activity and tyrosinase enzyme inhibition with IC50 value of 16.68 mg/ml and 741.62 mg/ml, respectively. Conclusion: This study demonstrated that the serum gel of AK leaf extract has potential as a skin lightening agent and is safe for use as a topical preparation.

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