scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF COCOA POD THAT ASSOCIATED IN MALTODEXTRIN IN VARIOUS CONCENTRATION

2018 ◽  
Vol 5 (2) ◽  
pp. 123
Author(s):  
Asriani Hasanuddin ◽  
Chairil Anwar ◽  
Marhawati Mappatoba ◽  
Hafsah Hafsah
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Natural Food ◽  
Skin Extract ◽  
E Coli ◽  
Dpph Method ◽  
Antioxidant And Antimicrobial Activity ◽  
Peel Extract

Cocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives.

scholarly journals Antimicrobial, antifungal and antioxidant activities of bee glue ethanol and aqueous extracts

2018 ◽  
Vol 90 (2) ◽  
Author(s):  
Nabi Shariatifar ◽  
Akram Janghorban ◽  
Rezvan Rahimnia ◽  
Amir Sasan Mozaffari Nejad
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Aqueous Extracts ◽  
E Coli ◽  
Food Borne ◽  
Dpph Method

Food-borne pathogens is a group of micro-organisms that cause food-borne illness, thus the research for finding effective drugs against this infection is necessary. The purpose of this study determines antibacterial, antioxidant activity antifungal of bee glue ethanol and aqueous extracts. Antibacterial and antifungal activities of extracts were evaluated against food-born (Escherichia coli, Staphylococcus aureus, Bacillus cereus, Vibrio cholerae and Candida albicans) purchased from Boali hospital on by the well diffusion method. Broth serial dilution method was doing to find the Minimal Inhibitory Concentration (MIC) and the agar dilution method to find the Minimal Bacterial Concentration (MBC) of extracts. The antioxidant activity of the extracts assaying by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Ethanol extract of bee glue was found the most effective against S. aureus strains (inhibition zone=27±0.87 mm) than E. coli strains (inhibition zone=5.5±0.37mm). Aqueous extract of propolis was found the most effective against S. aureus strains (inhibition zone=14.5±0.75 mm) than E. coli strains (inhibition zone=3.5±0.5mm) and C. albicans (inhibition zone=10±0.5 mm). The MIC and MBC values of the extracts ethanol and aqueous most effective against B. cereus strains were 0.3125, 0.625, 0.3125 and 1.25 mg/mL than E. coli strains 0.625, 1. 25, 2.5 and 50 mg/mL. and also for C. albicans was 0.625, 1.25, 1.25 and 2.5 mg/mL. IC50 ethanol and aqueous extracts of propolis were 8±0.25 and 45±1.75, respectively. This study was to assess the Antibacterial, antifungal and antioxidant activities activities of bee glue extracts in in vitro.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR

2020 ◽  
pp. 78-84
Author(s):  
FAVIAN BAYAS-MOREJON ◽  
ANGELICA TIGRE ◽  
RIVELINO RAMON ◽  
DANILO YANEZ
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Potassium Phosphate ◽  
Natural Antioxidants ◽  
Butylated Hydroxytoluene ◽  
Penicillin G ◽  
Diffusion Method ◽  
E Coli ◽  
Antibacterial And Antioxidant Activity

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals Polyphenolics profile effects upon the antioxidant and antimicrobial activity of propolis extracts

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mădălina Maria Nichitoi ◽  
Ana Maria Josceanu ◽  
Raluca Daniela Isopescu ◽  
Gabriela Olimpia Isopencu ◽  
Elisabeta-Irina Geana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Diffusion Method ◽  
Type Model ◽  
E Coli ◽  
Antimicrobial Efficiency ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging ◽  
Antibacterial And Antifungal ◽  
The Relationship

AbstractPropolis, a complex bee product, is a source of numerous bioactive principles, beneficial for human health, therefore it is intensively studied. In the present work, extracts of propolis from Bihor Romanian County were studied to identify the relationship between the polyphenolic derivatives profile and their antioxidant and antimicrobial activity. Extracts were obtained using water and 25%, 50%, and 70% ethanolic solutions (w/w), at 2:1, 4:1, and 6:1 liquid: solid ratios (w/w). 21 polyphenolic derivatives were quantified by UHPLC-MS, proving that the extracts composition strongly depends on the solvent. The sum of quantified polyphenolics extracted varied between 1.5 and 91.2 mg/g propolis. The antioxidant capacity was evaluated using the free radicals 2,2’-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging methods. Antimicrobial efficiency was tested against Gram-positive (B. subtilis), Gram-negative bacteria (E. coli), and fungi (C. albicans) by disc-diffusion method. All extracts, even the aqueous ones, demonstrated antibacterial and antifungal activity. Chemometric methods (partial least squares) and a saturation-type model were used to evaluate the contribution of various bioactive principles in building the antioxidant capacity of extracts. Both experimental and modelling results show that 50% ethanolic extracts provide a rich polyphenolics profile and ensure a good antioxidant capacity.

Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria

2020 ◽  
Vol 16 (2) ◽  
pp. 109-114
Author(s):  
Souad Khebri ◽  
Naima Benkiki ◽  
Mouloud Yahia ◽  
Hamada Haba
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Β Carotene

Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil. Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates. Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system. Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.

scholarly journals Didymocarpous Pedicellatus R. Br.: Qualitative and Quantitative GCMS Approach for Quality Control in Traditional Poly-Herbal Formulation with In Vitro Antioxidant and Antimicrobial Activity

2019 ◽  
Vol 35 (2) ◽  
pp. 648-657 ◽  
Author(s):  
Wasim Ahmad ◽  
Ennus T. Tamboli ◽  
Abuzer Ali ◽  
Mohd Amir ◽  
S. M. A. Zaidi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Good Precision ◽  
Gram Negative ◽  
Herbal Formulation ◽  
Antioxidant And Antimicrobial Activity ◽  
Bacteria And Fungi

A Gas Chromatography and Maas Spectroscopy method was developed and validated for determination and identification of α-humulene in a traditional medicinal herb Didymocarpous pedicellatus R. Br. (DP) (Gesneriaceae) and its poly herbal formulation, Safoof-e-pathar phori (SPP) including their essential oil. Hydrodistillation method were used for the isolation of essential oils from the leaves of DP and SPP herbal formulation. The proposed analysis method was found to be linear r2 = 0.999 in a wide concentration range (0.1-1000 µgmL-1), with good precision (RSD < 2.0%). Antimicrobial activity of α-humulene, DP and SPP oil was assessed by using agar well diffusion method against clinically important Gram-positive, Gram-negative bacteria and fungi. α-Humulene was found to be more active in contrast to Gram-negative bacteria while DP oil and SPP oil were exhibited maximum inhibition against fungal strains. Antioxidant activity of α-humulene, DP oil and SPP oil were determined using 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH) 96 well plate method, which showed antioxidant activity in the following order: standard ascorbic acid (DPPH FRS50 = 2.40 µg) > DP oil (DPPH FRS50 = 3.68 µg) > SPP oil (DPPH FRS50 = 12.54 µg) > and α-humulene (DPPH FRS50 = 36.19 µg).

scholarly journals Antibacterial and Antioxidant Activities of Polyeugenol with High Molecular Weight

2020 ◽  
Vol 20 (3) ◽  
pp. 722
Author(s):  
Erwin Abdul Rahim ◽  
Nur Istiqomah ◽  
Gilang Almilda ◽  
Ahmad Ridhay ◽  
Ni Ketut Sumarni ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Molecular Weight ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
E Coli ◽  
Ic50 Value ◽  
Inhibition Zones ◽  
Escherichia Coli Bacteria

This study was aimed to prepare polyeugenol with high molecular weight and to evaluate its antibacterial and antioxidant activities. First, polyeugenol was synthesized from eugenol in the presence of H2SO4-CH3COOH (4:1) as catalyst. The synthesized polyeugenol was weighed by using viscometer, revealing its high molecular weight of (7.76–21.9) × 105 g/mol. Furthermore, the antibacterial activity of the polyeugenol was conducted against Staphylococcus aureus and Escherichia coli bacteria. It was conducted by applying well diffusion method at 1, 2, 3, 4 and 5% concentrations to observe inhibition zones, in which the tests showed that the antibacterial activity of the polyeugenol against S. aureus were 17.42, 17.76, 18.79, 21.42 and 22.55 mm, while those against E. coli were 15.87, 17.23, 17.56, 18.24 and 19.21 mm, respectively. In short, these results indicated a strong antibacterial activity. Then, tests on antioxidant activity against free radical DPPH (2,2-diphenyl-1-pycrylhydrazyl) gave the IC50 value of 80.47 µg/mL, indicating a strong antioxidant activity. Therefore, the polymer synthesized in this work has a high potential to be applied in various biomedical applications.

scholarly journals Biosynthesis of selenium nanoparticles from Bacillus species and its applications

2019 ◽  
Vol 11 (4) ◽  
pp. 810-815 ◽  
Author(s):  
B. C. Greeshma ◽  
M. Mahesh
Keyword(s):  
Antioxidant Activity ◽  
Colour Change ◽  
Sem Analysis ◽  
Selenium Nanoparticles ◽  
Diffusion Method ◽  
Bacillus Species ◽  
Experimental Conditions ◽  
E Coli ◽  
Ft Ir ◽  
Antioxidant And Antimicrobial Activity

Selenium nanoparticles have been widely used in the various areas especially in medical field for its anticancer and immune modulatory properties. To reduce the toxic chemicals released by chemical process, the present work was aimed at synthesis of selenium nanoparticles by microbes. In this study, microbes were isolated from soil samples from different areas of Bangalore and screened for the antioxidant activity by DPPH assay. The organism with highest antioxidant activity (IC50 value = 11.6µg/mL) was identified as Bacillus species. Under experimental conditions, this microbe along with sodium selenite synthesised selenium nanoparticle indicated by the colour change of the medium to reddish orange. The synthesised selenium nanoparticles were further characterised. From UV-Vis spectrophotometry, the maximum peak was obtained at 266.5nm. The FT-IR analysis showed peaks at different wavelengths with the maximum of 3200cm-1 showing the presence of alcoholic group. The shape and size of the selenium nanoparticles was also calibrated by SEM analysis as oval and 209nm-748nm respectively. The nanoparticles were further analysed for antimicrobial assays by well diffusion method against E. coli, S. aureus, P. aeruginosa, St. mutans and antifungal assay against the strains of C. albicans, A. niger and A. flavus. The highest zone of inhibition was observed against E.coli (1.7 cm) at a concentration of 400µg and maximum for C. albicans (2.5cm) at a concentration of 400µg. Microbial synthesized nanoparticles emerges as a promising medicine in curing different disorders because of its potent antioxidant and antimicrobial activity.  

In Vitro Comparative Study on the Antibacterial and the Antioxidant Activity of Pergularia tomentosa L.

2021 ◽  
pp. 285-291
Author(s):  
Souad Belakehal ◽  
Brahim Labed ◽  
Louiza Zenkhri ◽  
Khedidja Benzahi ◽  
Ahmed Tabchouche
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Constituents ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
A Value ◽  
Antioxidant And Antimicrobial Activity

Ethanolic extracts of Pergularia tomentosa L. were used for the relative analysis of antioxidant and antimicrobial activity against two types of Gram-negative bacteria. (Escherichia coli G (-) and Pseudomonas aeruginosa G (-)) and a gram positive (Staphylococus aureus G (+)). Maximum activity was recorded against Escherichia coli, with a maximum inhibition diameter of 23mm using the disk diffusion method. In addition, the results revealed a higher bacterial activity against Escherichia coli with an inhibition diameter of 23mm compared to standard antibiotics Ampicillin/Nystatin with an inhibition diameter of 11.75mm. The study of the antioxidant activity by the DPPH and FRAP methods showed that vitamin C is the most active with an IC50 equal to 0.010 g/ml, followed by the crude extract, with a value of 0.012 g/ml and finally Tocopherol (VE) which seems to be the least effective with an IC50 of 0.015 g/ml. The results obtained in the present study suggest that Pergularia tomentosa L. can be used in the treatment of diseases caused by the organisms tested. Further chemical and pharmacological investigations can be carried out to isolate and identify the chemical constituents in selected plants responsible for antimicrobial activity.

scholarly journals Influence of Deep Eutectic Solvents (DESs) on Antioxidant and Antimicrobial Activity of Seed Extracts of Selected Citrus Species

2020 ◽  
pp. 120-128
Author(s):  
Lamija Kolarević ◽  
Emir Horozić ◽  
Zahida Ademović ◽  
Broza Šarić- Kundalić ◽  
Darja Husejnagić
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ionic Liquids ◽  
Lactic Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Diffusion Method ◽  
Antioxidant And Antimicrobial Activity ◽  
Seed Extracts

Deep eutectic solvents (DESs), as an alternative to ionic liquids, have greener credentials than ionic liquids and have attracted increasing attention in many applications. Choline chloride-based DESs in combination with different hydrogen bond donors (organic acid, sugars and urea), showed a high extraction efficiency. The aim of this study was to examine the possible effects of choline chloride-based eutectic solvents on the antioxidant and antimicrobial activity of lemon and mandarin orange seed extracts. Lactic acid, glucose, urea and water were used to prepare choline chloride-based Natural Deep Eutectic Solvents (NADESs). Ferric-Reducing Antioxidant Power (FRAP) Assay and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method were used to determine antioxidant activity. Antibacterial activity was investigated by diffusion method on reference bacterial strains E. coli, E. faecalis, S. aureus, B. subtilis and L. monocytogenes. The analysis revealed a significant reduction potential of the eutectic solvent based on lactic acid as well as significant antioxidant activity of lemon and mandarin seed extracts. In general, mandarin extracts showed better antioxidant capacity. In vitro antibacterial activity assays showed a complete absence of bacterial growth inhibition of the extracts. However, eutectic solvents with lactic acid have shown a significant antimicrobial effect.

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