Fatty Acid Profile of Synbiotic Cheese and its Effect on Intestinal Inflammation in Rats

2019 ◽  
Vol 14 (1) ◽  
pp. 45-50
Author(s):  
Nurliyani ◽  
Harmayani Eni ◽  
Rahmatulloh Satyaguna ◽  
Rakasivi Kanita Galih Julia
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Fatty Acid ◽  
Lactic Acid Bacteria ◽  
Fatty Acid Profile ◽  
Intestinal Inflammation ◽  
Lactobacillus Rhamnosus ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Chain Fatty Acids

Porang (Amorphophallus oncophyllus) is a local perennial plant rich in glucomannan. The objective of this study was to determine the effect of porang glucomannan addition during cheese processing on fatty acid profile, organic acid and vitamin B6 of goat milk cheese ripened with Lactobacillus rhamnosus. In addition, the effect of cheese consumption on short-chain fatty acid profile in the caecum digesta of inflammatory rats was evaluated. We found that the addition of glucomannan to the cheese during its ripening increased the levels of myristic, pentadecanoic acid, and cis-oleic acids. Rats consuming this cheese had elevated cecal levels of propionic, butyric, total short-chain fatty acids, and lactic acid bacteria. Consumption of synbiotic cheese also decreased the intestinal inflammation via increasing the total lactic acid bacteria, propionic, butyric, and total short-chain fatty acids.

scholarly journals 118 Awardee Talk - Genomic control of fatty acid profile in beef

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 108-108
Author(s):  
Steven D Shackelford ◽  
Andy King ◽  
Warren Snelling ◽  
Tara G McDaneld ◽  
Larry A Kuehn ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Short Chain Fatty Acids ◽  
The United States ◽  
High Energy ◽  
Short Chain ◽  
Genomic Control ◽  
A Genome ◽  
Chain Fatty Acids

Abstract Steers (n = 868) were raised, feedlot-finished with ad libitum access to a high-energy diet and harvested to determine if there is genomic control of fatty acid profile traits in beef breeds common to the United States. Cattle included purebred and crossbred progeny of Angus, Red Angus, Hereford, Shorthorn, Simmental, Charolais, Limousin, Gelbvieh, Maine Anjou, Chiangus, Braunvieh, Salers, Brahman, Brangus, Santa Gertrudis, and Beefmaster as well as three composite lines. Either directly or through imputation, genotypes were determined for > 133,000 functional single nucleotide polymorphisms (SNP). At approximately 38 h postmortem, a 2.54-cm-thick longissimus steak was obtained from the 13th rib region of the left side of each carcass. At 14 d postmortem, steaks were cooked and subsequently longissimus was pulverized in liquid nitrogen. Fatty acid profile was determined by gas chromatography and mass spectrometry. A genome-wide association study was conducted for fatty acid profile traits expressed as a deviation from the contemporary group mean using the Mixed Linear Model Analysis of SNP & Variation Suite 8.8.3 (Golden Helix) and Pre-computed Kinship Matrix using the GBLUP Genomic Relationship Matrix. A SNP in coiled coil domain containing 57 (CCDC57; Chromosome 19 at 51,349,695) affected the percentage of C14:0 (P < 10–46), short-chain fatty acids (P < 10–36), and saturated fatty acids (P < 10–17). Also, a SNP in thyroid hormone responsive (THRSP; Chromosome 29 at 18,090,403) affected the percentage of C14:0 (P < 10–16) and short-chain fatty acids (P < 10-10). The percentage of polyunsaturated fatty acids was affected by SNP in myostatin (Chromosome 2 at 6,213,980; P < 10–15). These results show that fatty acid profile of beef can be changed through genetic selection but, it is not clear if the level of change will be great enough to impact human health.

scholarly journals Naturally Acquired Lactic Acid Bacteria from Fermented Cassava Improves Nutrient and Anti-dysbiosis Activity of Soy Tempeh

2021 ◽  
Vol 9 (A) ◽  
pp. 1148-1155
Author(s):  
Rio Kusuma ◽  
Jaka Widada ◽  
Emy Huriyati ◽  
Madarina Julia
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gut Microbiota ◽  
Dietary Fibre ◽  
Short Chain Fatty Acids ◽  
Diabetic Animal ◽  
Short Chain ◽  
Microbiota Composition ◽  
Chain Fatty Acids

Introduction: Gut microbiota dysbiosis indicated by increased gram-negative bacteria and reduced Firmicutes-producing short chain fatty acids bacteria has been linked with impairment in glucose metabolism. Tempeh is traditional fermented soy food that can stimulate the growth of beneficial bacteria. In Indonesia, some tempeh was produced by adding acidifier that contains lactic acid bacteria. This process may impact the nutrient and anti-dysbiosis activity of tempeh.   Objectives: To evaluate the impact of acidifier on nutrient and gut microbiota profile of diabetic animal model.  Method: Modified tempeh was made by addition of water extract of fermented cassava. Standard and modified tempeh were subjected to proximate analysis and dietary fibre. Diabetic animals were received standard tempeh or modified tempeh diet replacing 15% and 30% of protein in the diet for 4 weeks of intervention. At the end of experiment, caecal content was collected. Short chain fatty acids and microbiota composition were analysed using 16s rDNA next generation sequencing (NGS). Result: There is significant different (p<0.05) on fat, protein, water and dietary fibre content between regular soy tempeh and modified tempeh. There is significant different (p<0.05) on serum glucose and short chain fatty acid composition among group. Diabetic animal has low ratio of Firmicutes/Bacteroidetes. Supplementation of both tempeh increased bacterial diversity, Firmicutes /Bacteroidetes ratio and short chain fatty acids producing bacteria.   Conclusion: Addition of naturally occurred lactic acid bacteria from fermented cassava during tempeh processing improved both nutrient and microbiota composition in the gut of diabetes mellitus.  

Variable Effects of Short Chain Fatty Acids and Lactic Acid in Inducing Intestinal Mucosal Injury in Newborn Rats

2002 ◽  
Vol 35 (4) ◽  
pp. 545-550 ◽  
Author(s):  
Jing Lin ◽  
Suhas M. Nafday ◽  
Sara N. Chauvin ◽  
Margret S. Magid ◽  
Sudha Pabbatireddy ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Mucosal Injury ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Newborn Rats ◽  
Intestinal Mucosal Injury ◽  
Chain Fatty Acids

scholarly journals Stimulation of fetal hemoglobin production by short chain fatty acids

Blood ◽  
1995 ◽  
Vol 86 (8) ◽  
pp. 3227-3235 ◽  
Author(s):  
E Liakopoulou ◽  
CA Blau ◽  
Q Li ◽  
B Josephson ◽  
JA Wolf ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Globin Gene ◽  
Fetal Hemoglobin ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Erythroid Cell ◽  
Gamma Globin ◽  
Carbon Fatty Acid ◽  
Chain Fatty Acids

Abstract Butyrate, a four-carbon fatty acid, and its two-carbon metabolic product, acetate, are inducers of gamma-globin synthesis. To test whether other short-chain fatty acids share this property, we first examined whether propionic acid, a three-carbon fatty acid that is not catabolized to acetate, induces gamma-globin expression. Sodium propionate increased the frequency of fetal hemoglobin containing erythroblasts and the gamma/gamma + beta mRNA ratios in adult erythroid cell cultures and F reticulocyte production in a nonanemic juvenile baboon. Short-chain fatty acids containing five (pentanoic), six (hexanoic), seven (heptanoic), eight (octanoic), and nine (nonanoic) carbons induced gamma-globin expression (as measured by increase in gamma-positive erythroblasts and gamma/gamma + beta mRNA ratios) in adult erythroid burst-forming unit cultures. There was a clear-cut relationship between the concentration of fatty acids in culture and the degree of induction of gamma-globin expression. Three-, four-, and five-carbon fatty acids were better inducers of gamma globin in culture as compared with six- to nine-carbon fatty acids. These results suggest that all short-chain fatty acids share the property of gamma-globin gene inducibility. The fact that valproic acid, a derivative of pentanoic acid, also induces gamma-globin expression suggests that short-chain fatty acid derivatives that are already approved for human use may possess the property of gamma-globin inducibility and may be of therapeutic relevance to the beta-chain hemoglobinopathies.

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