scholarly journals Phenolic compounds in two subspecies of Drypis spinosa L. (Caryophyllaceae) in Croatia

2020 ◽  
Vol 80 (1) ◽  
Author(s):  
Dario Kremer ◽  
Iztok Jože Košir ◽  
Tanja Potočnik ◽  
Nikolina Rogulj ◽  
Karla Načinović ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Rosmarinic Acid ◽  
Hplc Analysis ◽  
Sinapic Acid ◽  
Total Flavonoids ◽  
Total Polyphenols ◽  
Spectrophotometric Methods ◽  
First Time

As a contribution to the chemotaxonomic relations, the quantitative analysis of bioactive phenolic compounds was carried out for the first time in Drypis spinosa L. subsp. spinosa and D. spinosa subsp. jacquiniana Murb. et Wettst. ex Murb. in Croatia. Total polyphenols (TP), tannins (T) and total flavonoids (TF) were determined in the samples of leaves, stems, and roots using UV-Vis spectrophotometric methods. For the subsp. spinosa, the highest content of TF was in leaves (0.09%), as well as the highest amount of TP (2.36%) and T (1.12%). In the subsp. jacquiniana, the highest content of TF (0.10%), TP (1.96%), and T (0.88%) was measured in stems. Coumaric, ferulic and rosmarinic acid were identified and quantified by HPLC analysis in both subspecies. Quercetin and sinapic acid were identified only in subsp. spinosa, while rutin and naringenin were found only in subsp. jacquiniana. Among them, ferulic acid was identified only in flowers of both subspecies. The results of this study represent a useful basis for further research of phytochemical and eventually phytotherapeutic potential of D. spinosa.

scholarly journals Phenolic compounds variation in Mentha L. Species in the course of a four-years period / Kolísanie fenolových látok v rôznych druhoch Mentha L. Počas 4 ročného obdobia

2015 ◽  
Vol 62 (s9) ◽  
pp. 2-7
Author(s):  
S. Fialová ◽  
D. Tekeľová ◽  
K. Rendeková ◽  
J. Klinčok ◽  
M. Kolárik ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Pharmacological Properties ◽  
European Pharmacopoeia ◽  
Total Polyphenols ◽  
Spectrophotometric Methods ◽  
Hydroxycinnamic Derivatives

AbstractMints rank among the most important Lamiaceae plants. In addition to essential oil, they contain many valuable phenolic compounds, including flavonoids and phenolic acids that participate in mints’ pharmacological properties. In this work, we examined the contents of phenolic compounds variation in the course of 4 years of vegetation. We compared the contents of total hydroxycinnamic derivatives expressed as rosmarinic acid (λ = 505 nm), total polyphenols and tannins expressed as rosmarinic acid (λ = 760 nm), and flavonoids expressed as luteolin-7-O-glucoside (λ = 392 nm) and quercetin (λ = 420 nm) in 1-, 2-, 3-, and 4-year-old plants’ dry leaves, respectively. Spectrophotometric methods of the European Pharmacopoeia were employed. Our results show high levels of active phenolic compounds, particularly in 3- and 4-year-old plants.

scholarly journals The effects of plant density and irrigation on phenolic content in cauliflower 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 178-185 ◽  
Author(s):  
Alina Kałużewicz ◽  
Jolanta Lisiecka ◽  
Monika Gąsecka ◽  
Włodzimierz Krzesiński ◽  
Tomasz Spiżewski ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Plant Density ◽  
Phenolic Content ◽  
Sinapic Acid ◽  
Dry Weight ◽  
Coumaric Acid

This study was conducted to study the influence of plant density and irrigation on the content of phenolic compounds, i.e., phenolic acids and flavonols in cv. ‘Sevilla’ cauliflower curds. Levels of phenolic acids and flavonols were in the range of 3.0–6.2 mg and 25.4–87.8 mg/100 g of dry weight, respectively, depending on plant density and irrigation. Of the phenolic acids, caffeic acid was detected in the highest amount, followed by p-coumaric acid, sinapic acid, gallic acid, and ferulic acid. Of the two flavonols detected, the levels of quercetin were higher than those of kaempferol. The content of the detected phenolic acids (with the exception of ferulic acid) and both flavonols increased with increasing plant density. Furthermore, the concentration of phenolic compounds (with the exception of ferulic acid) was significantly higher under irrigation.

Oxidative Stability of Edible Vegetable Oils Enriched in Polyphenols with Olive Leaf Extract

2007 ◽  
Vol 13 (6) ◽  
pp. 413-421 ◽  
Author(s):  
F.N. Salta ◽  
A. Mylona ◽  
A. Chiou ◽  
G. Boskou ◽  
N.K. Andrikopoulos
Keyword(s):  
Phenolic Compounds ◽  
Oxidative Stability ◽  
Antioxidant Capacity ◽  
Hplc Analysis ◽  
Leaf Extract ◽  
Olive Leaf ◽  
Total Polyphenols ◽  
Olive Leaf Extract ◽  
Rancimat Method ◽  
Oil Sunflower

Commercially available oils (olive oil, sunflower oil, palm oil, and a vegetable shortening) were enriched in polyphenols, by adding olive leaf extract. Addition of the extract was performed in such way that the oils were enriched with 200 mg/kg polyphenols. Total polyphenols of both enriched and commercial oils were estimated by the Folin—Ciocalteau assay, while identification and quantification of individual simple phenolic compounds was performed by GC/MS and of oleuropein by HPLC analysis. The enrichment resulted in the supplementation of the commercial oils mainly with oleuropein, hydroxytyrosol, and quercetin. Antioxidant capacity and oxidative stability of the enriched oils and the commercial ones were assessed by the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging assay and the Rancimat method, respectively. Both antioxidant capacity and oxidative stability were substantially improved for all the oils studied after supplementation. By the procedure adopted, oils rich in polyphenols, mainly in oleuropein, can be produced with olive leaf extract supplementation.

scholarly journals Monitoring of the PAL Enzymatic Activity and Polyphenolic Compounds in Leaves and Fruits of Two Myrtle Cultivars during Maturation

Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 389
Author(s):  
Silvia Medda ◽  
Leonarda Dessena ◽  
Maurizio Mulas
Keyword(s):  
Phenolic Compounds ◽  
Phenylalanine Ammonia Lyase ◽  
Raw Materials ◽  
Phenylpropanoid Pathway ◽  
Polyphenolic Compounds ◽  
Antioxidant Compounds ◽  
Total Polyphenols ◽  
Spectrophotometric Methods ◽  
Pal Activity ◽  
Potential Use

The leaves and berries of myrtle (Myrtus communis L.) are rich in phenolic compounds, such as phenolic acids, flavonoids, and flavanols. The richness of these antioxidant compounds allows the potential use of myrtle biomasses as raw materials for medicinal and functional food products. Most of the phenolic compounds originate from the phenylpropanoid pathway, where phenylalanine ammonia lyase (PAL) enzyme activates the first step. The objective of this research is to study the activity of PAL as related to accumulation in the myrtle fruits and leaves of some phenolic compounds in the period between blossom and full berry ripening. With this aim, we compared two model genotypes with different fruit coloration. In leaves and berries of two cultivars, ‘Giovanna’ with pigmented berries and ‘Grazia’ with white berries, the PAL activity and content of polyphenols, anthocyanins, flavonoids, and tannins were determined with spectrophotometric methods. PAL activity was quite constant in leaves and variable in berries: Greater in berries of ‘Giovanna’ than in those of ‘Grazia’ cultivar, and increasing from berry color-break to full ripening. In berries, a positive correlation between PAL and flavonoids (r = 0.44), and between PAL and anthocyanins (r = 0.69), as well as a negative correlation between PAL and total polyphenols (r = −0.471), were found.

scholarly journals Simultaneous Quantification of Diarylheptanoids and Phenolic Compounds in Juglans mandshurica Maxim. by UPLC–TQ-MS

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 132
Author(s):  
Hong Yang ◽  
Li-Bo Wang ◽  
Ya-Ping Guo ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Juglans Regia ◽  
Folk Medicine ◽  
Total Content ◽  
Antitumor Activities ◽  
Juglans Mandshurica ◽  
Intrinsic Quality ◽  
First Time

The immature epicarps of Juglans mandshurica and Juglans regia have been used as folk medicine for the treatment of cancer in China. Other parts of the J.mandshurica plant, including leaves, branches, barks, and stems, have reported antitumor activities. We previously found that various diarylheptanoids and phenolic compounds isolated from J. mandshurica epicarps show significant antitumor activities. However, there are no reports of quantitative analysis of diarylheptanoids and phenolic compounds of J. mandshurica. In this study, a validated quantitative method, based on ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, was employed to determine the contents of eight diarylheptanoids and seven phenolic compounds in the epicarps of J. mandshurica during different growth periods, in different parts of the plant, and in the epicarps of two Juglans species. The most successful J. mandshurica epicarp harvesting time fell between Day 12 and Day 27. The leaves of J. mandshurica showed potential for medical use as they had the highest content of the 15 compounds (3.399 ± 0.013 mg/g). We showed for the first time that the total content of diarylheptanoids in J. mandshurica is higher than that in J. regia, though, conversely, J. regia has higher contents of phenolic compounds. The method developed in this study is practical and simple and can be applied for quantitative analysis for evaluating the intrinsic quality of J. mandshurica.

scholarly journals Effect of in vitro gastrointestinal digestion on phenolic compounds and the antioxidant activity of Aloe vera

2020 ◽  
Vol 7 (3) ◽  
pp. 11-25
Author(s):  
Imen Laib ◽  
Farida Kehal ◽  
Nour Elyakine Haddad ◽  
Taous Boudjemia ◽  
Malika Barkat
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Aloe Vera ◽  
Total Phenol Content ◽  
Gastrointestinal Digestion ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Total Polyphenol ◽  
Spectrophotometric Methods

AbstractThe aim of this work is to study the effect of digestion on the total polyphenol content, flavonoids and the antioxidant activity of Aloe vera. Total polyphenol contents and flavonoid spectrophotometric methods: The evaluation of the antioxidant activity was carried out by three methods, DPPH, ABTS and CUPRAC. To confirm the results obtained we carried out an analysis by ATR-FTIR. The total phenol content found in the Aloe vera extract studied was 1.3638 mg EAG/100 g, while the content of flavonoids found in the Aloe vera extract studied was 0.690 mg EQ/100 g. The values of total polyphenols and flavonoids decreased under the effect of gastrointestinal digestion. The spectra obtained during the ATR-FTIR analysis show that Aloe vera is rich in phenolic compounds and flavonoids. Intense bands corresponding to O–H bonds, C=C bond, C–H, CO, CH3 and CH2 confirm the presence of these bioactive compounds. For both the DPPH and CUPRAC methods, Aloe vera extract reveals a strong antioxidant activity, which gradually decreases during the oral and gastric phase and then increases after the intestinal digestion. For the ABTS method, the antioxidant activity decreases during the oral phase, increases during the gastric phase and then decreases again during the intestinal phase.

Antioxidant activity of ginger extract and identification of its active components

2015 ◽  
Vol 8 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Silvia Mošovská ◽  
Dominika Nováková ◽  
Michal Kaliňák
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Cation Radical ◽  
Zingiber Officinale ◽  
Dpph Radical ◽  
Nmr Analysis ◽  
Active Components ◽  
Ginger Extract ◽  
Total Polyphenols ◽  
Spectrophotometric Methods

Abstract The object of presented study was the evaluation of antioxidant activity of extract from Zingiber officinale. A method for the preparation of the extract was chosen, which enabled to isolate mostly phenolic compounds. According to the measurements with Folin-Ciocalteu reagent, obtained extract comprised total polyphenols (181.41 mgGAE/g of extract) from which flavonoids contributed to 7.8 % (14.15 mgquercetin/g of extract). Nuclear magnetic resonance (NMR) analysis showed that the phenolic ketones were predominant in the extracts of whole phenolic compounds. According to results of two spectrophotometric methods (ABTS and DPPH test), ginger extract showed stronger ability to scavenge DPPH radical than ABTS cation radical.

scholarly journals Pharmacognostic and physicochemical evaluation, HPLC analysis and antiproliferative properties of Kigelia africana (Lam.) Benth

2021 ◽  
Vol 19 (2) ◽  
pp. 410-428
Author(s):  
O.T. Fatokun ◽  
L. Omorogbe ◽  
A. Adamu ◽  
K.B. Esievo ◽  
S.E. Okhale
Keyword(s):  
Phenolic Compounds ◽  
Moisture Content ◽  
Calcium Oxalate ◽  
Ferulic Acid ◽  
Caffeic Acid ◽  
Hplc Analysis ◽  
Stem Bark ◽  
Calcium Oxalate Crystals ◽  
Root Extracts ◽  
Oxalate Crystals

Introduction/Objective of study: Kigelia africana (Bignoniaceae) is enriched with bioactive constituents and has thus found various uses in African folklore. This study aims to evaluate the pharmacognostic, physicochemical, chromatographic and antiproliferative properties of K. africana.Methodology: Standard methods were used to determine the qualitative microscopy, moisture content, ash and extractive value. Furthermore, HPLC analysis was conducted on the samples in order to detect and quantify some phenolic compounds (gallic acid, chlorogenic acid, rutin, ferulic acid, caffeic acid and quercetin). The Sorghum bicolor model was used for the antiproliferative assay. All experiment was carried out in triplicates.Results: Microscopy revealed amphistomata with the presence of non-glandular unicellular, uniseriate trichomes on K. africana leaf. Cellulose, tannins, calcium oxalate crystals on the leaf and stem bark, while the roots lacked calcium oxalate crystals. Ash contents were leaf (21.8 ± 0.1) %w/w, stem bark (4.8 ± 0.03)%w/w and root (3.9 ± 0.2)%w/w. Moisture content was (10.5 ± 0.5) %w/w and (9.5 ± 0.2) %w/w for the root and leaf parts, respectively. All values were within WHO limits for crude drugs. The stem bark and root parts contained more water-soluble constituents than alcohol soluble constituents. From the results of HPLC analysis the leaf, stem bark and root extracts gave 24 peaks, 16 peaks and 30 peaks, respectively, a few peaks matched with reference compounds- quercetin, caffeic acid, ferulic acid and rutin. Results of antiproliferative assay showed that methotrexate was significantly (p ˂ 0.05) more effective than the stem bark (from 2-64 mg/mL) with inhibitions ranging from 72.0 ± 1.4% - 90.0 ± 2.4% and root extracts (from 4 – 64 mg/mL) that had inhibitions ranging from 50.3 ± 1.5% - 97.7 ± 0.4% but comparable with leaf extract (from 16 mg/mL - 64 mg/mL) with inhibitions ranging from 68.4 ± 0.8% - 99.0 ± 0.1%.Conclusion: Further information which can be included in an official monograph of the plant for its proper identification and quality control has been provided by this study. Kigelia africana exhibited effective antiproliferative activities and the presence of phenolic compounds.

scholarly journals Quantification of some secondary metabolites in selected Cornaceae species / Stanovenie vybraných sekundárnych metabolitov v niektorých druhoch čeľade Cornaceae

2015 ◽  
Vol 62 (s9) ◽  
pp. 8-11 ◽  
Author(s):  
V. Forman ◽  
M. Haladová ◽  
D. Grančai
Keyword(s):  
Secondary Metabolites ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Plant Material ◽  
European Pharmacopoeia ◽  
Total Polyphenols ◽  
Spectrophotometric Methods ◽  
Cornus Mas ◽  
Cornus Kousa ◽  
Hydroxycinnamic Derivatives

Abstract Leaves of selected species of the Cornaceae family (Swida alba, Swida australis, Swida controversa, Swida coreana, Swida baileyi, Cornus kousa, Cornus mas) were investigated in this study. These shrubs or small trees with hard wood are cultivated in Slovakia (Tesarske Mlyňany Arboretum). Some species (C. mas and C. kousa) have edible fruits. Cornels find broad ethnopharmacological use, for example, in Traditional Chinese Medicine (TCM), where fruits of C. officinalis are used for the treatment of several disorders, such as organ hypersecretion or diabetes. They are also used as tonics and for astringent effects. Leaves and fruits of C. kousa have been reported to possess antioxidative, anti-inflammatory and antidiabetic properties. The aim of our study is to compare the content of selected secondary metabolites groups in the leaves of above-mentioned species. The plant material was collected in the Tesarske Mlyňany Arboretum, Slovakia, in September 2010. Modified spectrophotometric methods of the European Pharmacopoeia, 8th edition (Ph. Eur. 8) were used for all assays. Results show that the total hydroxycinnamic derivatives (THD) content varied from 1.14 (S. alba) to 2.86% (S. coreana) when expressed as rosmarinic acid, and from 1.01 (S. alba) to 2.55% (S. coreana) when expressed as caffeic acid. The flavonoids content ranged from 0.21 (C. mas) to 1.37% (S. coreana), total polyphenols content from 9.6 (C. kousa) to 7.27% (S. coreana), and tannins content from 7.17 (C. kousa) to 4.04% (S. coreana). Leaves of Korean dogwood (S. coreana) were observed to show the highest content of THD expressed as rosmarinic acid (2.86%) and caffeic acid (2.55%), as well as of flavonoids (1.37%). Kousa dogwood (C. kousa) contained the highest amounts of tannins (7.17%) and total polyphenols (9.6%) on the other hand.

scholarly journals Phenolic compounds and in vitro antioxidant activity of six accessions of mashua (Tropaeolum tuberosum R. & P.) from Puno Region, Peru

2021 ◽  
Vol 74 (3) ◽  
pp. 9707-9714
Author(s):  
Haim Behar Montes ◽  
Oscar Reategui ◽  
Danae Marcela Liviac Muñoz ◽  
Jesús Heráclides Arcos Pineda ◽  
Iván Karlos Best Cuba
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Total Content ◽  
Total Flavonoids ◽  
Total Polyphenols ◽  
Tropaeolum Tuberosum ◽  
In Vitro Antioxidant Activity

Mashua (Tropaeolum tuberosum R. & P.) is an Andean crop of high nutritional value and medicinal properties, which presents a great diversity in morphology and color. The aim of the study was to evaluate the content of phenolic compounds and in vitro antioxidant activity of the most economically important mashua accessions in the Puno Region, Peru. Six accessions of mashua (three purplecolored and three yellow-colored) were evaluated. The content of total polyphenols, total flavonoids and identification of phenolic compounds was determined by the Folin-Ciocalteu assay, aluminum chloride colorimetric method and HPLC-DAD, respectively. In vitro antioxidant activity was evaluated using the FRAP and DPPH assays. In general, the purple-colored mashua had a significantly higher content of total polyphenols, total flavonoids, and in vitro antioxidant activity compared to the yellowcolored mashua; being the Tt-23 accession purple-colored (peel/pulp, purple/purple), which presented a significantly higher content of phenolic compounds and in vitro antioxidant activity compared to the other accessions evaluated (P<0.05). Furthermore, a significant correlation was observed between FRAP and DPPH activities with the total content of polyphenols and flavonoids (P<0.01), as well as between FRAP activity and the caffeic acid and rutin levels (P<0.05). These results suggest that purple-colored mashua, particularly the Tt-23 accession (peel/pulp, purple/purple), has better nutraceutical and antioxidant properties due to its higher content of phenolic compounds.

Sign in / Sign up

Export Citation Format

Share Document