scholarly journals Simultaneous Quantification of Diarylheptanoids and Phenolic Compounds in Juglans mandshurica Maxim. by UPLC–TQ-MS

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 132
Author(s):  
Hong Yang ◽  
Li-Bo Wang ◽  
Ya-Ping Guo ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Juglans Regia ◽  
Folk Medicine ◽  
Total Content ◽  
Antitumor Activities ◽  
Juglans Mandshurica ◽  
Intrinsic Quality ◽  
First Time

The immature epicarps of Juglans mandshurica and Juglans regia have been used as folk medicine for the treatment of cancer in China. Other parts of the J.mandshurica plant, including leaves, branches, barks, and stems, have reported antitumor activities. We previously found that various diarylheptanoids and phenolic compounds isolated from J. mandshurica epicarps show significant antitumor activities. However, there are no reports of quantitative analysis of diarylheptanoids and phenolic compounds of J. mandshurica. In this study, a validated quantitative method, based on ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, was employed to determine the contents of eight diarylheptanoids and seven phenolic compounds in the epicarps of J. mandshurica during different growth periods, in different parts of the plant, and in the epicarps of two Juglans species. The most successful J. mandshurica epicarp harvesting time fell between Day 12 and Day 27. The leaves of J. mandshurica showed potential for medical use as they had the highest content of the 15 compounds (3.399 ± 0.013 mg/g). We showed for the first time that the total content of diarylheptanoids in J. mandshurica is higher than that in J. regia, though, conversely, J. regia has higher contents of phenolic compounds. The method developed in this study is practical and simple and can be applied for quantitative analysis for evaluating the intrinsic quality of J. mandshurica.

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

scholarly journals HPLC study of phenolic compounds in Mirabilis jalapa raw material

Pharmacia ◽  
2020 ◽  
Vol 67 (3) ◽  
pp. 145-152
Author(s):  
Dhurgham Khalid Abed Sarray ◽  
Liliia M. Horiacha ◽  
Iryna O. Zhuravel ◽  
Andrii I. Fedosov
Keyword(s):  
Phenolic Compounds ◽  
Folk Medicine ◽  
Total Content ◽  
Hplc Method ◽  
Raw Material ◽  
Control Methods ◽  
Quantitative Content ◽  
Different Types ◽  
Mirabilis Jalapa ◽  
Hplc Study

Mirabilis jalapa is a popular decorative plant valued for its beautiful multicolored flowers. Folk medicine in various countries applies Mirabilis jalapa as anti-microbial, anti-inflammatory, diuretic, spasmolytic drug. Chemical composition of different types of Mirabilis jalapa has not yet been adequately studied which is an obstacle for its application in medicine. The qualitative composition and quantitative content of phenolic compounds were studied by the HPLC method. The performed experiment revealed presence of hydroxycinnamic acids, flavonoids, isoflavonoids and coumarins in tested herb. The content of phenolic compounds was the highest in Mirabilis jalapa flowers, counting as much as 2977.41 ± 59.55 µg/mg. Total content of phenolic compounds in Mirabilis jalapa herb was 304.25 ± 6.08 µg/mg, in fruits – 67.92 ± 1.36 µg/mg, and in roots – 12.44 ± 0.25 µg/mg. Quantitatively neochlorogenic acid dominated in flowers, chlorogenic acid in fruits, whereas Mirabilis jalapa herb mostly contained rutin and hyperoside. The obtained results will be useful in the development of quality control methods for Mirabilis jalapa herb and manufacture of drug preparations on its basis.

scholarly journals HPLC determination of phenolic compounds content in Parmelia sulcata and Parmelia vagans thalli

Pharmacia ◽  
2019 ◽  
Vol 66 (4) ◽  
pp. 161-164
Author(s):  
O. A. Kyslychenko ◽  
Viktoriia V. Protska ◽  
Iryna O. Zhuravel
Keyword(s):  
Phenolic Compounds ◽  
Inflammatory Diseases ◽  
Raw Materials ◽  
Folk Medicine ◽  
Usnic Acid ◽  
Total Content ◽  
Raw Material ◽  
Parmelia Sulcata ◽  
Quantitative Content

The species of Parmelia genus have long been used in Indian folk medicine for the treatment of bronchitis, ulcers, furunculosis, cardiovascular diseases, urolithiasis, amenorrhea, and also at infectious and inflammatory diseases. In Ukraine, the most common lichens of the Parmelia genus are Parmelia sulcata Tailor and Parmelia vagans Nyl. At the same time, thalli of Parmelia genus lichens belong to the non-officinal and poorly studied types of raw material. The qualitative composition and the quantitative content of phenolic compounds in Parmelia sulcata and Parmelia vagans thalli was studied by HPLC. According to the results of the chromatographic analysis, salazinic, fumaroprotocetraric, usnic acids, chloratranorin and atranorin were identified in both types of raw material studied. In addition, protocetraric acid was identified in Parmelia sulcata thalli. According to the results of the experiment, the total content of identified phenolic compounds in Parmelia sulcata thalli was 2019.71±40.39 g/mol, and in Parmelia vagans thalli it comprised 1754.18±34.77 g/mol. In the thalli of both studied species of Parmelia genus, fumaroprotocetraric acid dominanted by the quantity. This substance was present in Parmelia sulcata thalli in the amount of 474.00±9.00 g/mol, and in Parmelia vagans thalli – 456.21±8.67 g/mol. In addition, a significant amount of chloratranorin (408.79±8.99 g/mol) was present in Parmelia sulcata thalli. Quite a high content of atranorin (393.34±8.65 g/mol) and usnic acid (375.31±7.53 g/mol) were defined in Parmelia vagans thalli. The results obtained can be used in the development of quality control methods for Parmelia sulcata and Parmelia vagans thalli, as well as medicines based on these types of raw materials.

scholarly journals The Influence of the Extraction Conditions of Blackthorn (Prunus spinosa) Fruits and Leaves on the Yield of Phenolic Compounds

2021 ◽  
pp. 49-56
Author(s):  
Nino Gamkrelidze ◽  
◽  
Giorgi Kvartskava ◽  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Plant Extracts ◽  
Food Production ◽  
Total Content ◽  
Dry Weight ◽  
Extraction Methods ◽  
Prunus Spinosa ◽  
Quality Of Food

Plant extracts used in food production significantly improve the quality of food. Chemical composition is the main prerequisites for the final use of plant extracts. The chemical composition of the extracts is affected by the extraction method and conditions - temperature, duration and solvent. On the basis of experimental data optimal conditions for the extraction of phenolic compounds from the fruits and leaves of blackthorn (Prunus spinosa) were selected. Among the extraction methods used in the experiment, the highest content of phenolic compounds in obtained extracts was observed during the ultrasonic extraction. A mixture of 0.25 parts of water and 0.75 parts of 96% ethanol showed best results from different volume ratios of water and ethanol. The optimal extraction time was 90 minutes. And the optimum temperature is 45°C. The total content of phenolic compounds in the extracts obtained by the abovementioned method amounted is 35.62±0.17 mgg -1(dry weight) for blackthorn fruits and 44,76 ± 0.08mgg -1(dry weight) for the leaves.

Bioactive Compounds from the Flower Part of Polygonum maritimum L. Collected from Algerian Coast

2020 ◽  
Vol 16 (4) ◽  
pp. 543-545 ◽  
Author(s):  
Imad Abdelhamid El Haci ◽  
Wissame Mazari ◽  
Fawzia Atik-Bekkara ◽  
Fatma Mouttas-Bendimerad ◽  
Fayçal Hassani
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Local Database ◽  
Rp Hplc ◽  
Algerian Coast ◽  
First Time

Background: Polygonum maritimum is one of the spontaneous halophyte plants of the Algerian coast. Many studies were carried out to evaluate the contents and the quality of phenolic compounds of this plant around the Mediterranean region. Objective: This paper intends to identify, for the first time, the phenolic compounds from the flower part of P. maritimum. Methods: RP-HPLC-PDA (Reversed Phase-High Performance Liquid Chromatography-Photo Diode Array) material was used for this purpose. Many standards were used and their retention times were stored in a local database. Identification was made on the basis of retention times of retained compounds and those found in the literature, and UV spectra of each peak. Results: This study intends to identify five phenolic acids (gallic, ferulic, sinapic, caffeic and syringic acids), one flavonol (rutin) and one flavanone (naringenin). Conclusion: P. maritimum is an important source of natural bioactive compounds that can be exploited for the benefit of many fields.

scholarly journals Phenolic Compounds from Centaurea horrida L Growing in Lebanon

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Rima Boukhary ◽  
Maha Aboul-ElA ◽  
Othman Al-Hanbali ◽  
Abdalla El-Lakany
Keyword(s):  
Phenolic Compounds ◽  
Middle East ◽  
Ethyl Acetate ◽  
Caffeic Acid ◽  
Folk Medicine ◽  
Isolation And Identification ◽  
Phytochemical Investigation ◽  
First Time

Centaurea horrida has been used in folk medicine for many purposes as diuretic, antiinflammatory, hypotensive, antidiarrhetic, mild astringent, bitter tonic, stomachic, digestive, and emmenagogue and in cosmetics. An infusion of this plant is used to treat diabetes and digestive problems Also it is used as a wash for tired eyes and in conjunctivitis. C.horrida grown in Lebanon is widely used in the treatment of diarrhea, as antihypertensive and as hypoglycemic. Phytochemical investigation of the species grown in Lebanon led to the isolation and identification of five phenolic compounds isolated from methanol, ethyl acetate and butanol fractions of Centaurea extracts. Their structures were determined by NMR, MS, UV and IR methods. It is important to mention that the isolated flavonoids fisetin, hispidulin, quercetin, quercetin -3-D- galactoside and caffeic acid were isolated for the first time from Centaurea horrida. L growing in the Middle East.

scholarly journals Lovage (Levisticum officinale W.D.J. Koch) Roots: A Source of Bioactive Compounds towards a Circular Economy

Resources ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 81
Author(s):  
Rafael Mascoloti Spréa ◽  
Ângela Fernandes ◽  
Tiane C. Finimundy ◽  
Carla Pereira ◽  
Maria José Alves ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Circular Economy ◽  
Folk Medicine ◽  
Thiobarbituric Acid ◽  
Biological Properties ◽  
Hexane Extract ◽  
Pharmaceutical Industries ◽  
Hydroethanolic Extract ◽  
First Time

Lovage (Levisticum officinale W.D.J. Koch) is an aromatic plant from the Apiaceae (Umbelliferae) family used as a condiment in several regions of Europe and also described to have medicinal properties. While the aerial parts are used as foods, the roots are generally discarded. In the past, lovage roots were used in folk medicine for their diuretic, carminative, and spasmolytic properties. Therefore, the exploitation of this undervalued part of the plant can be a source of valuable bioactive compounds for food and/or pharmaceutical industries. Thus, in this study, extracts of different polarity were prepared and studied regarding their chemical composition and bioactive properties. To the best of our knowledge, the composition in phenolic compounds and the volatile profile of the n-hexane extract are reported for the first time. A total of 24 compounds were identified by GC-MS in the n-hexane extract, evidencing a high relative abundance of phthalides. A total of eight phenolic compounds were identified in lovage root extracts (decoction and hydroethanolic extract), with vanillic acid being the major compound. Regarding antioxidant activity, also reported for the first time, decoction and hydroethanolic extract exhibited a high antioxidant capacity in thiobarbituric acid reactive substances (TBARS) (179 ± 11 μg/mL) and in oxidative hemolysis (OxHLIA) assays (510 ± 6 μg/mL), respectively. n-Hexane extract showed relevant anti-proliferative activity against all tumor cell lines tested (GI50, 48–69 μg/mL), despite inhibiting also the growth of a non-tumoral hepatocyte cell line, however, presenting a significantly higher GI50 value (147 μg/mL). This study revealed that lovage root, an agri-food residue, can be a source of valuable bioactive compounds also presenting biological properties that deserve being explored, which could lead to a circular economy for food and/or the pharmaceutical industry.

scholarly journals Phenolic compounds in two subspecies of Drypis spinosa L. (Caryophyllaceae) in Croatia

2020 ◽  
Vol 80 (1) ◽  
Author(s):  
Dario Kremer ◽  
Iztok Jože Košir ◽  
Tanja Potočnik ◽  
Nikolina Rogulj ◽  
Karla Načinović ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Rosmarinic Acid ◽  
Hplc Analysis ◽  
Sinapic Acid ◽  
Total Flavonoids ◽  
Total Polyphenols ◽  
Spectrophotometric Methods ◽  
First Time

As a contribution to the chemotaxonomic relations, the quantitative analysis of bioactive phenolic compounds was carried out for the first time in Drypis spinosa L. subsp. spinosa and D. spinosa subsp. jacquiniana Murb. et Wettst. ex Murb. in Croatia. Total polyphenols (TP), tannins (T) and total flavonoids (TF) were determined in the samples of leaves, stems, and roots using UV-Vis spectrophotometric methods. For the subsp. spinosa, the highest content of TF was in leaves (0.09%), as well as the highest amount of TP (2.36%) and T (1.12%). In the subsp. jacquiniana, the highest content of TF (0.10%), TP (1.96%), and T (0.88%) was measured in stems. Coumaric, ferulic and rosmarinic acid were identified and quantified by HPLC analysis in both subspecies. Quercetin and sinapic acid were identified only in subsp. spinosa, while rutin and naringenin were found only in subsp. jacquiniana. Among them, ferulic acid was identified only in flowers of both subspecies. The results of this study represent a useful basis for further research of phytochemical and eventually phytotherapeutic potential of D. spinosa.

scholarly journals Walnut (J. regia) Agro-Residues as a Rich Source of Phenolic Compounds

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 535
Author(s):  
Aljaz Medic ◽  
Jerneja Jakopic ◽  
Anita Solar ◽  
Metka Hudina ◽  
Robert Veberic
Keyword(s):  
Phenolic Compounds ◽  
Juglans Regia ◽  
Rich Source ◽  
Phenolic Contents ◽  
Persian Walnut ◽  
Phenolic Profiles ◽  
Hydroxybenzoic Acids ◽  
First Time ◽  
By Products ◽  
Agro Residues

The present study was designed to identify and quantify the major phenolic compounds (phenolics) in the inner and outer husks, buds and bark of the Persian walnut, Juglans regia L. A comparison across six different cultivars grown in Slovenia was also carried out: ‘Fernor’, ‘Fernette’, ‘Franquette’, ‘Sava’, ‘Krka’ and ‘Rubina’. A total of 83 compounds were identified, which included 25 naphthoquinones, 15 hydroxycinnamic acids, 8 hydroxybenzoic acids, 13 flavanols, 2 flavones, 1 flavanone and 19 flavonols. For the first time, 38 phenolics in the husks, 57 phenolics in the buds and 29 phenolics in the bark were presented in J. regia within this study. Naphthoquinones were the major phenolics determined, approximately 75% of all analysed phenolics in the inner husk, 85% in the outer husk, 50% in buds and 80% in bark. The highest content of phenolics was found in the walnut buds, followed by the bark, the inner husk and the outer husk. On the basis of these high phenolic contents, walnut husks, buds and bark represented valuable by-products of the walnut tree. These data also show origin-related phenolic contents across the cultivars, and thus these phenolic profiles might serve to define the origins of different walnut cultivars.

scholarly journals Quantitative Analysis of Phenolic Compounds and Mineral Contents of Rosa canina L. Waste Seeds

2021 ◽  
Vol 9 (6) ◽  
pp. 1120-1123
Author(s):  
Kadriye Özlem Saygı
Keyword(s):  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Inductively Coupled Plasma ◽  
Folk Medicine ◽  
Atomic Emission ◽  
Emission Spectrometry ◽  
Plasma Atomic Emission Spectrometry ◽  
Mineral Contents ◽  
Rosa Canina ◽  
Inductively Coupled

Natural products play an important role in medicine. They have been used extensively in folk medicine to treat various illnesses. In this work, quantitative analysis of phenolic compounds in methanol, acetonitrile and dichloromethane extracts of Rosa canina L. waste seeds were investigated by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) LC-MS/MS. Mineral analysis of R. canina seeds was determined by inductively coupled plasma–atomic emission spectrometry (ICP-OES). Fe, Mn, K and Zn were found as chief elements. Quantitative analysis revealed that catechin was the major flavonoid in all extracts. This work offers a viewpoint for recycling the R. canina waste seeds into the economy due to their bioactive content.

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