scholarly journals Study of acute toxicity of GIZh-298

2021 ◽  
pp. 48-54
Author(s):  
I. A. Miroshkina ◽  
A. V. Sorokina ◽  
A. V. Volkova ◽  
V. V. Zabrodina ◽  
I. V. Alekseev ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Clinical Picture ◽  
Toxic Substances ◽  
Male Mice ◽  
Control Groups ◽  
Starch Solution ◽  
Preclinical Research ◽  
Internal Organs ◽  
Experimental Animals ◽  
Lethal Doses

Assessment of acute toxicity is a necessary stage of preclinical research of the substance GIZh-298. The aim of present research is study of acute toxicity GIZh-298. Methods. GIZh-298 was administered once intraperitoneally to mice at doses 200-330 mg/kg. Equivalent volume of 1 % starch solution was administered to animals of the control groups. Periods of intoxication and death of animals with a detailed description of the observed clinical picture were registered. Euthanasia and pathoanatomical dissection were performed 14 days after drug administration. Results. The median lethal doses were identified: LD50 = 299,6 (279,7 – 320,8) mg/kg in female mice, LD50 = 302,3 (281,5 – 324,6) mg/kg in male mice at intraperitoneal introduction. The morphological view of the internal organs, detected during pathoanatomical dissection of all surviving experimental animals, did not differ from that observed in control animals. Conclusion. It was determined that GIZh-298 at intraperitoneal introduction concerns to low-toxic substances. According to classification Sidorov K.K. GIZh-298 may be related to 4th toxicity class.

scholarly journals Determination of acute toxicity of the ‘Bondarmin’ disinfectant

2019 ◽  
Vol 5 (2) ◽  
pp. 26-30
Author(s):  
A. O. Bondarchuk ◽  
A. P. Paliy ◽  
M. Ye. Blazheyevskiy
Keyword(s):  
Acute Toxicity ◽  
Toxic Effect ◽  
Toxicity Assessment ◽  
Laboratory Animals ◽  
Intragastric Administration ◽  
Toxic Substances ◽  
Internal Organs ◽  
National University ◽  
Scientific Laboratory ◽  
High Doses

In the article the results of the study of toxic effect of the designed disinfectant (active ingredient potassium peroxomonosulfate) on laboratory animals (mice) are presented. For the recent years a variety of scientific works both by domestic and by foreign scientists has been devoted to the study of different disinfectants’ toxicity. However today there is a number of issues that require more detailed studying and scientific justification. Among them the problem of toxic effects of disinfectants on the animal organism occupies a special place. The aim of our work was to study the toxic effect on the laboratory animals and to assess the acute toxicity (LD50) of the designed ‘Bondarmin’ disinfectant. Tests were carried out at the Laboratory of pharmacology and toxicology of the National University of Pharmacy (Kharkiv) and on the base of Educational and scientific laboratory of genetic and molecular research methods named after P. I. Verbitskiy in the Kharkiv State Zooveterinary Academy. Acute toxicity assessment (LD50) was carried out with intragastrointestinal administration of the designed drug to laboratory animals (mice). Changes in the internal organs of animals that were removed from the experiment for humane reasons and those who died after the experiment were detected by macroscopic examination. The lethality of laboratory animals after the intragastric administration of disinfectant was determined by the Prozorovskiy method The dynamic of changes in body weight of mice after the administration of disinfectant in high doses (from 1,500 to 3,500 mg/kg) was found out. The influence of the disinfectant on the mass coefficients of the internal organs of male mice after intragastric administration was evaluated. Toxic effect of the designed disinfectant ‘Bondarmin’, when using intragastric method of administration to laboratory animals (mice), according to the age and sexual index (LD50 = 2,702.40 ± 156.32 mg/kg), was established. Disinfectant ‘Bondarmin’ refers to IV toxicity class (low toxic substances).

scholarly journals Toxicopathological study of aqueous extract of beetle cocoon Larinus maculatus F. on some internal organs in male albino mice

2012 ◽  
Vol 36 (0E) ◽  
pp. 315-321
Author(s):  
Iqbal Naji Tawfiq
Keyword(s):  
Oral Administration ◽  
Aqueous Extract ◽  
Mus Musculus ◽  
Folk Medicine ◽  
Low Doses ◽  
Undesirable Effect ◽  
Male Mice ◽  
Pathological Changes ◽  
Internal Organs ◽  
Experimental Animals

The present study was carried out to investigate the effect of oral administration of hot aqueous extract of beetle cocoon Larinus maculatus Faldermann, in a two doses 50and100mg/Kg/Bw for 25 and 45days respect- tively on some organs such as liver, lung, kidney, intestine, heart, spleen, and brain in male mice Mus musculus. The results indicated that there were toxicopathological changes in many tissues of experimental animals. Histo -pathological changes was dose and period dependent . It was found that the aqueous extract of beetle cocoon has undesirable effect at the administered doses, since the raw extract of this cocoon is currently being used in Folk medicine as treatment for cough, bronchitis in Iraq. This study revealed that the low doses of this extract have some deleterious effects on certain organs, so it should not be used for long durations in control of respiratory disease.

scholarly journals Biological activity of new 6-[(1-naphthylmethyl)thio)]-4-chloropyrazolo[3,4-d]pyrimidines

2020 ◽  
Vol 20 (1-2) ◽  
pp. 146-151
Author(s):  
Ekaterina S. Ofitserova ◽  
Lilia N. Alekseeva ◽  
Artem A. Shklyarenko ◽  
Igor P. Yakovlev
Keyword(s):  
Acetic Acid ◽  
Biological Activity ◽  
Acute Toxicity ◽  
Analgesic Activity ◽  
White Male ◽  
Generation Model ◽  
Toxic Substances ◽  
Male Mice ◽  
Reference Drug ◽  
Non Linear

Topicality. This article presents the results of the study of the biological activity of newly synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines. The acute toxicity of 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines was determined experimentally on non-linear white male mice with a total weight of 1620 g. A generation model was used to experimentally evaluate the analgesic activity acetic writhing in male mice. It was experimentally confirmed that the new synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines were non-toxic and had pronounced analgesic activity. Aim: to study the biological activity of the new synthesized 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines. Materials and methods. The prediction of the likely types of biological activity was carried out with the use of PASS computer program. The acute toxicity of 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines was determined experimentally on non-linear white male mice. Analgesic activity was established on a model of acetic acid writhing in mice. Results. 6-[(1-naphthylmethyl)thio]-4-chloropyrazolo[3,4-d]pyrimidines were classified as non-toxic substances (LD50 2000 mg / kg). The analgesic activity of the test compounds was higher than that of the reference drug. Conclusion. The new synthesized 6-[(1-naphthylmethyl]thio)-4-chloropyrazolo[3,4-d]pyrimidines are non-toxic and have pronounced analgesic activity.

scholarly journals Determination of the parameters of acute toxicity of «Brovermectin-granulate» on earlings carp

2017 ◽  
Vol 19 (74) ◽  
pp. 20-23
Author(s):  
Yu. V. Loboiko ◽  
M. M. Danko ◽  
O. V. Krushelnytska ◽  
S. I. Kravets
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Oral Administration ◽  
General Condition ◽  
Lethal Dose ◽  
Toxic Substances ◽  
Starch Solution ◽  
Method Of Calculation ◽  
Anterior Intestine

The paper presents the results of research to determine the parameters of acute toxicity of «Brovermectin-granulate». The material for the study of acute toxicity were of carp earlings. In experiment used drug «Brovermectin-granulate» (1 g of the drug contains: active actiion substance ivermectin – 3.5 mg, tocopherol acetate – 20 mg). The introduction of the drug  was carried out orally using a probe in anterior intestine of fish. The drug was administered to fish in the form of a homogeneous suspension produced 1 % starch solution individually, in doses of 1000, 4000, 8000, 12000, 16000 and 20000 mg/kg of body weight. For settings acute toxicity determined the general condition and death of fish; for a dose of the drug (DL50) calculated by methods G. Kerber, H. Pershyn, least squares analysis of probit mortality curves for V.B. Prozorovskyj, means three points for B.M. Shtabskyj. At this stage of research it was found lethal (DL100) and maximum tolerated (DL0) dose of «Brovermectin-granulate» for earlings carp. As a result of studies found that 100% death of fish following the dose of 20000 mg/kg (DL100), and for the drug at a dose of 4000 mg/kg (DL0) set to survival of 100% fish. Established that the value of average lethal dose of «Brovermectin-granulate» for fish regardless of the method of calculation mainly coincided and were 10932,8–11200 mg/kg for earlings carp. Thus, the drug «Brovermectin-granulate» for oral administration to fish belongs to grade 4 toxicity – low toxic substances.

scholarly journals STUDY OF THE «ACUTE» TOXICITY OF LIPOSOMAL DRUG FORM OF ANTINEOPLASTIC PHOTOSENSITIZER THIOSENS

2015 ◽  
Vol 14 (3) ◽  
pp. 49-54 ◽  
Author(s):  
N. Yu. Kulbachevskay ◽  
E. V. Sanarova ◽  
O. I. Konyeva ◽  
N. P. Ermakova ◽  
V. M. Buchman ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Dosage Form ◽  
Dose Range ◽  
Clinical Signs ◽  
Male Rats ◽  
Male Mice ◽  
Preclinical Research ◽  
Male And Female ◽  
Liposomal Drug ◽  
Series Of Experiments

The laboratory for development of drug forms, N.N. Blokhin Russian Cancer Research Center developed a lyophilized liposomal dosage form (LLDF) of a new infrared photosensitizer phthalocyanine derivative - Thiosens. Previous studies determined optimal composition of the dosage form, technology of its production and methods of analysis; a number of biological experiments were performed. The next step of preclinical research is studying "acute" toxicity of LLDF, which is presented in this paper. The study of "acute" toxicity of LLDF Thiosens was carried out in the laboratory of Pharmacology and Toxicology. "Acute" toxicity of LLDF Thiosens was studied in the experiments on mice hybrids F1 (CBA » C57Bl / 6J) male and female and weinbrener outbred male rats. In the first series of experiments LLDF Thiosens was administered to male mice in the dose range of 5; 10; 20 and 30 mg/kg. In the second series of experiments, male and female mice were injected with LLDF Thiosens in the dosage range 6; 12 and 24 mg/kg. Male mice were treated by LLDF Thiosens intravenously; no symptoms of toxicity were registered at the doses of 5 and 10 mg/kg; death was observed in all experimental animals after administration at the doses of 20 and 30 mg/kg on the first day in the result of inactivity and piloerection. In the second series of experiments no sex differences were found in single intravenous injection of LLDF Thiosens. The results of the studies of "acute" toxicity in rats demon strated that when LLDF Thiosens was administered in doses 3, 6 and 9 mg/kg animal deaths were reported. A single intravenous LLDF Thiosens administration in rats in the excess of the expected dose for human in 1.5-4.5 times was well tolerated by the animals: animal mortality, clinical signs of toxicity and changes in behavioral responses were absent.

ACUTE TOXICITY OF OCTENIDINE HEXAFLUOROSILICATE

2021 ◽  
Author(s):  
I. O. Shyshkin ◽  
V. Yu. Anisimov ◽  
A. V. Nikitin ◽  
V. O. Gelmboldt
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Least Squares Method ◽  
Lethal Dose ◽  
Statistical Processing ◽  
Toxic Substances ◽  
White Rats ◽  
Relative Safety ◽  
Lethal Doses

The aim of the work. Determination of toxicometric characteristics of octenidine hexafluorosilicate (OHFS), characterized by significant pharmaceutical potential, in an acute experiment on rats by oral administration. Materials and Methods. A study of the acute toxicity of octenidine hexafluorosilicate was carry out on 42 male Wistar rats weighing 180–200 grams. The main criterion for quantifying the toxicity of octenidine hexafluorosilicate was LD50, which was determined using the least squares method. In addition, the following hazard indicators were calculated: 1/LD50 – median lethal dose (absolute toxicity), LD84/LD16 – the range of lethal doses (zone of acute toxic effect), 1/(LD50-S) – the total toxicity index and the S-function angle of inclination (variability of lethal doses). Statistical processing of the results was carry out using the «StatPlus 2009» software (AnalystSoft, USA, 2009). Results and Discussion. The results of the acute toxicity determination of octenidine hexafluorosilicate show that this compound, in the oral route of administration, belongs to the III class of toxicity for the human (slightly hazardous) and to the IV class of toxicity for the animals (white rats). Based on the variability of lethal doses, the studied hexafluorosilicate can be attribute to compounds that do not pose a high potential risk of the onset and development of poisoning. The calculated toxicity and hazard values of octenidine hexafluorosilicate show that it does not pose a particular danger to humans. Extrapolation to humans of acute toxicity parameters obtained in animals was determined using the coefficient of resistance to the species and is 132.15 mg/kg body weight. Conclusions. The results of determining the toxicometric characteristics of octenidine hexafluorosilicate in rats by oral administration allow to classify this compound as moderately toxic substances (LD50 = 555.05 mg / kg, toxicity class IV). The determined parameter of acute toxicity of OHFS is close to the LD50 values of other hexafluorosilicates known from the literature; relative safety and high caries-prophylactic and periodontal-protective efficacies of OHFS indicate the prospects for further studies of this compound.

scholarly journals Acute Toxicity of Cu-MOF Nanoparticles (nanoHKUST-1) towards Embryos and Adult Zebrafish

2021 ◽  
Vol 22 (11) ◽  
pp. 5568
Author(s):  
Natalia Abramenko ◽  
Gregory Deyko ◽  
Evgeny Abkhalimov ◽  
Vera Isaeva ◽  
Lyubov Pelgunova ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Toxic Effect ◽  
Biomedical Applications ◽  
Copper Ions ◽  
Adult Zebrafish ◽  
Internal Organs ◽  
Copper Release ◽  
Transmission Electron ◽  
Concentration Dependency ◽  
Metal Organic

Metal-organic frameworks (MOFs) demonstrate unique properties, which are prospective for drug delivery, catalysis, and gas separation, but their biomedical applications might be limited due to their obscure interactions with the environment and humans. It is important to understand their toxic effect on nature before their wide practical application. In this study, HKUST-1 nanoparticles (Cu-nanoMOF, Cu3(btc)2, btc = benzene-1,3,5-tricarboxylate) were synthesized by the microwave (MW)-assisted ionothermal method and characterized by X-ray powder diffraction (XRD) and transmission electron microscopy (TEM) techniques. The embryotoxicity and acute toxicity of HKUST-1 towards embryos and adult zebrafish were investigated. To gain a better understanding of the effects of Cu-MOF particles towards Danio rerio (D. rerio) embryos were exposed to HKUST-1 nanoparticles (NPs) and Cu2+ ions (CuSO4). Cu2+ ions showed a higher toxic effect towards fish compared with Cu-MOF NPs for D. rerio. Both forms of fish were sensitive to the presence of HKUST-1 NPs. Estimated LC50 values were 2.132 mg/L and 1.500 mg/L for zebrafish embryos and adults, respectively. During 96 h of exposure, the release of copper ions in a stock solution and accumulation of copper after 96 h were measured in the internal organs of adult fishes. Uptake examination of the major internal organs did not show any concentration dependency. An increase in the number of copper ions in the test medium was found on the first day of exposure. Toxicity was largely restricted to copper release from HKUST-1 nanomaterials structure into solution.

scholarly journals Determination of acute toxicity parameters of the drug ‘MEGASTOP for dogs’ on white rats and mice

2020 ◽  
Vol 6 (2) ◽  
pp. 20-26
Author(s):  
O. L. Orobchenko ◽  
M. Ye. Romanko ◽  
M. O. Yaroshenko ◽  
I. O. Gerilovych ◽  
N. A. Zhukova ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Clinical Symptoms ◽  
Liver Volume ◽  
Male Rats ◽  
Toxic Substances ◽  
Main Experiment ◽  
Polyethylene Glycol 400 ◽  
White Rats ◽  
Rats And Mice

The experiments were performed on 58 males of nonlinear white rats 3–4 months old and weighing 180–200 g and 64 females of nonlinear white mice 2.5–3 months old and weighing 18–22 g. In the main experiment on rats, six experimental groups were formed, the animals of which were injected intragastrically with the drug ‘MEGASTOP for dogs’ (by absolute weight) in doses of 1,000.0, 2,000.0, 3,000.0, 4,000.0, 5,000.0, and 6,000.0 mg/kg body weight; in the main experiment on mice, seven experimental groups were formed, the animals of which were administered the drug in doses of 100.0, 500.0, 1,000.0, 1,500.0, 2,000.0, 2,500.0, and 3,000.0 mg/kg body weight. Control rats and mice were injected with 2.0 cm3 and 0.2 cm3 of polyethylene glycol-400, respectively. Clinical symptoms of poisoning with the drug ‘MEGASTOP for dogs’ of white rats (at doses of 2,000.0–6,000.0 mg/kg body weight) and mice (at doses of 1,000.0–3,000.0 mg/kg body weight) were refusals of food and water, loss of coordination, sitting in one place, a dose-dependent increase in depression with subsequent complete depression, lack of response to external stimuli and death on the first or fourth day after administration. During autopsy in rats and mice that died as a result of poisoning with the drug ‘MEGASTOP for dogs’, we recorded pallor of the mucous membranes of the mouth, trachea, pharynx, and esophagus; increase in heart volume, atrial blood supply; pulmonary hyperemia; uncoagulated blood; increase in liver volume, dark cherry color, flabby consistency; catarrhal inflammation of the mucous membrane of the small intestine. According to the results of determining the parameters of acute toxicity of the drug ‘MEGASTOP for dogs’ in the case of a single intragastric injection, LD50 for male rats is 3,384.98 ± 444.94 mg/kg, and for female mice — 2,025.88 ± 279.46 mg/kg body weight, which allows to classify it to class IV by the toxicity — low-toxic substances (LD50 — 501–5,000 mg/kg) and by the degree of danger to class III— moderately dangerous substances (LD50 — 151–5,000 mg/kg)

scholarly journals Distribution of Acid-fast Bacilli in the Skin, Extremities and Internal Organs of Various Experimental Animals

Repura ◽  
1966 ◽  
Vol 35 (1) ◽  
pp. 27-32
Author(s):  
TATSUO MORI ◽  
KENJI KOHSAKA ◽  
YOSHIHARU KISHI ◽  
MASAXO KAMEI ◽  
SHINJI NISHIMURA
Keyword(s):  
Internal Organs ◽  
Experimental Animals ◽  
Acid Fast Bacilli

scholarly journals Ketoksikan Akut dari Ekstrak Etanolik Daun Jarak Pagar (Jatropa curcas) pada Mencit Jantan Galur Balb/C

2014 ◽  
Vol 15 (1) ◽  
pp. 52
Author(s):  
Hanif Nasiatul Baroroh ◽  
Eka Prasasti Nur Rachmani
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Mice ◽  
Animal Test ◽  
Group I ◽  
Liver And Kidney ◽  
Single Dosage ◽  
Histopathology Examination ◽  
Lung And Kidney

The acute toxicity of Jatropa curcas leaves on Balb/C male mice was studied in rats. This research aimed to determine acute toxicity, evaluate spectrum of toxic effect and mechanism that caused the death of animal test after administration of ethanolic extract of J. curcas leaves, single dosage orally on 24 hours observation. The research used male mice, which are divided into 5 groups. Group I was negative control with CMC-Na. Group II, III, IV, and V were given extract with dose of 1400 mg/kgBW, 2240 mg/kgBW, 3584 mg/kgBW and 5734 mg/kgBW, respectively. Evaluation of the toxic symptoms and death of animal test was done for 24 hours. If the animal test was died before 24 hours then it underwent surgery to take the heart, liver, lung, and kidney. In the end of the evaluation, all mice were killed to take the vital organs for histopathologic examination. No mortality was observed during study. The test resulted LD50 of ethanolic extract from J. curcas leaves using Balb/C male mice was 5734 mg/kg of BW. It was categorized as practically not toxic. Administration of the extract did not cause alterations of animal behaviours. Histopathology examination shows inflammation in lung, liver, and kidney after administration of the extract.

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