Distribution of Acid-fast Bacilli in the Skin, Extremities and Internal Organs of Various Experimental Animals

The experiment with the chronic effects of alcohol on experimental animals reflects the picture of long-term use of alcoholic beverages by a person with alcoholism. It is impossible to fully study the ultrastructural changes in internal organs, including the reproductive system, in humans, both in acute and chronic alcohol consumption, but the results obtained in the experiment can be extrapolated to humans. The results obtained showed that changes in the parenchymal-stromal elements of the testicles during chronic alcoholization are reduced to dystrophic transformation of the spermatogenic epithelium. The testicular stroma is characterized by pronounced circulatory disorders with plethora and stasis in the postcapillaries and small veins, edema, phenomena of perivascular and interstitial sclerosis, with foci of pronounced hyalinosis, most expressed by 2-3 months of alcoholism. By 6 months of the model experiment, there was no dynamics of the growth of morphological changes, partial adaptation to constant intoxication with ethyl alcohol.

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A investigation of the influence of water-soluble polysaccharides of calamus root (Acorus calamus L.) introduction on the functions of experimental animals (pathomorphological aspects)

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2010-3-57-64 ◽

2010 ◽

Vol 9 (3) ◽

pp. 57-64

Author(s):

A. M. Guriyev ◽

M. V. Belousov ◽

R. R. Akhmedzhanov ◽

M. S. Yusubov ◽

A. A. Churin ◽

...

Keyword(s):

Body Mass ◽

The State ◽

Water Soluble ◽

Parenteral Administration ◽

Acorus Calamus ◽

Pathological Changes ◽

Internal Organs ◽

Experimental Animals ◽

Influence Of Water

The influence of the sum of water-soluble polysaccharides (WSPSs) extracted from sedge (Acorus calamus L.) rhizomes on the state of internal organs of rats and rabbits at a long parenteral administration has been studied. The intraperitoneal WSPS introduction to rats in doses of 20, 100, and 200 mg/kg of body mass for 3 months and the intravenous WSPS introduction to rabbits in doses of 25 and 50 mg/kg of body mass for 1 months caused no pathological changes in the morphology of internal organs and systems.

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Toxicological characteristics of a medicinal product for veterinary «Ketoprofen 10%» in laboratory rats (subchronic toxicity)

Veterinariya, Zootekhniya i Biotekhnologiya ◽

10.36871/vet.zoo.bio.202106006 ◽

2021 ◽

Vol 1 (6) ◽

pp. 34-41

Author(s):

A. N. Shkatova ◽

◽

D. A. Devrishov ◽

O. B. Litvinov ◽

V. E. Brylina ◽

...

Keyword(s):

Body Weight ◽

Medicinal Product ◽

Intramuscular Injection ◽

Study Drug ◽

The Body ◽

Subchronic Toxicity ◽

Internal Organs ◽

Experimental Animals ◽

Laboratory Rats

In a subchronic experiment with intramuscular injection in rats, it was found that a dose of 70,7 mg/kg is toxic, and doses of 35,4 and 17,2 mg/kg are inactive (safe). It was noted that the 14 day administration of the drug «Ketoprofen 10%» in a dose 70,7 mg/kg caused a decrease in the body weight of experimental animals, contributed to the development of hepatotoxic action, a change in the mass coefficients of internal organs. The above effects were reversible 10 days after discontinuation of the study drug.

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Peculiarities of the course of radiation-mechanical injuries during modeling of bone-muscular trauma of the limbs in expirement

Vestnik of Russian military medical Academy ◽

10.17816/brmma62810 ◽

2020 ◽

Vol 22 (4) ◽

pp. 82-86

Author(s):

S. I. Slukhay ◽

K. P. Golovko ◽

I. S. Drachev ◽

D. V. Remizov

Keyword(s):

Surgical Treatment ◽

Soft Tissues ◽

Morphological Changes ◽

Mechanical Damage ◽

The Body ◽

Acute Radiation ◽

Weapons Of Mass Destruction ◽

Internal Organs ◽

Septic Complications ◽

Experimental Animals

Experimental data on the course of the wound process and changes in the internal organs of animals during acute radiation sickness are presented. Morphological changes in the wound and with a fracture of the femur, on the model of combined radiation-mechanical conduction in 30 experimental animals (rats). It was found that wounds after primary surgical treatment often suppurate, and the performed osteosynthesis in fractures of tubular bones turns out to be an untenable delivery of metal structures. An unfavorable course of the wound process is noted in the form of pronounced purulent-necrotic changes in soft tissues, which leads to the appearance of degenerative-dystrophic changes in internal organs in experimental animals. The development of the infectious process in the wound, radiation damage is more severe, while the lethality increases by 2023%. The total area and thickness of the newly formed granulation tissue is 1,52 times less than in non-irradiated animals. As a consequence of the disturbance of reparative processes during the development of the mutual burdening syndrome, the consolidation of the fracture with radiation injury to rats was completed in 28,51,2 days, while in non-irradiated animals it was already by 2023 days. The reason for the death of animals is purulent-septic complications in 21 (73%) irradiated rats. Sepsis due to St. aureus was noted in 14 (66,6%) rats and St. epidermalis in 2 (9,5%) animals. Colibacillary sepsis was recorded in 5 (23,8%) rats. Thus, in the conditions of the use of weapons of mass destruction, radiation-mechanical damage will occupy one of the leading places in the structure of sanitary losses. At the same time, the simultaneous effect of radiation and a mechanical factor on the body forms one of the most dangerous variants of damage, in which a favorable outcome is predicted only in milder forms of acute radiation syndrome and provided that surgical treatment is timely.

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Study the Effect of Cladosporium Spp. on Some Internal Organs of Experimental Animals

Indian Journal of Public Health Research & Development ◽

10.5958/0976-5506.2019.02632.9 ◽

2019 ◽

Vol 10 (9) ◽

pp. 1343

Author(s):

Zainab R. Zghair

Keyword(s):

Internal Organs ◽

Experimental Animals

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Toxicopathological study of aqueous extract of beetle cocoon Larinus maculatus F. on some internal organs in male albino mice

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v36i0e.439 ◽

2012 ◽

Vol 36 (0E) ◽

pp. 315-321

Author(s):

Iqbal Naji Tawfiq

Keyword(s):

Oral Administration ◽

Aqueous Extract ◽

Mus Musculus ◽

Folk Medicine ◽

Low Doses ◽

Undesirable Effect ◽

Male Mice ◽

Pathological Changes ◽

Internal Organs ◽

Experimental Animals

The present study was carried out to investigate the effect of oral administration of hot aqueous extract of beetle cocoon Larinus maculatus Faldermann, in a two doses 50and100mg/Kg/Bw for 25 and 45days respect- tively on some organs such as liver, lung, kidney, intestine, heart, spleen, and brain in male mice Mus musculus. The results indicated that there were toxicopathological changes in many tissues of experimental animals. Histo -pathological changes was dose and period dependent . It was found that the aqueous extract of beetle cocoon has undesirable effect at the administered doses, since the raw extract of this cocoon is currently being used in Folk medicine as treatment for cough, bronchitis in Iraq. This study revealed that the low doses of this extract have some deleterious effects on certain organs, so it should not be used for long durations in control of respiratory disease.

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Influence of intraabdominal hypertension on morphological changes of internal organs in experiment

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2019-23(1)-01 ◽

2019 ◽

Vol 23 (1) ◽

pp. 6-11

Author(s):

A.I. Sukhodolya ◽

K.Yu. Krenov ◽

I.V. Loboda ◽

V.M. Monastyrsky

Keyword(s):

Morphological Changes ◽

Abdominal Cavity ◽

Intraabdominal Pressure ◽

Control Group ◽

Internal Organs ◽

Experimental Animals ◽

Abdominal Hypertension ◽

Wide Range ◽

Intraabdominal Hypertension ◽

Neutral Formalin

The development of intra-abdominal hypertension is possible with a wide range of surgical pathologies, in particular, the abdominal cavity, as well as in other nosologies and significantly aggravates the prognosis. Thus, polytrauma and major burns present up to 60% of the risk of abdominal compartment syndrome (AKS), and intestinal obstruction, peritonitis and acute destructive pancreatitis - in almost 80% of cases. The purpose of the work was to investigate the morphological changes that occur in the internal organs in an intraabdominal hypertension syndrome caused by peritonitis. The experiment was conducted on 28 guinea pigs, divided into three groups: I — the control group (n=8), the II group, in which the peritonitis was simulated by destruction of the colon in the experimental animals (n=10), III — the group of experimental animals, in which the peritonitis model was combined with a paranephrine blockade in combination with a blockage of the circular ligament of the liver (n=10). In all experimental animals, intraabdominal pressure was measured every 6 hours during the day. The results were processed using Microsoft Excel spreadsheet formulas using the Student t criterion. For morphological study section material was used (fragments of the kidneys, small intestine and peritoneum). To facilitate cutting the pieces, we carried out preliminary fixation of organs in 10% neutral formalin (at least 24 hours). From each organ, cut into 1–2 bits or ribbons, which were fixed in 10% neutral formalin solution. After posting according to the generally accepted method, bits were poured into paraffin. Paraffin sections 5–7 microns thick were stained with hematoxylin and eosin. As a result of experimental work, it has been found that combined regional blockades effectively reduce intra-abdominal pressure within six hours after use, but their effect is short-lived. In the experimental peritonitis group, with the implementation of regional blockades with lidocaine solution, the morphological changes of the kidneys and peritoneum were less pronounced. Improvement of the technique of regional blockades, in particular the continued infusion of local anesthetics, can significantly increase the effectiveness of the technique without side effects that are inherent in, in particular, peridural analgesia.

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An investigation of the introduction of water-soluble polysaccharides calamus root (Acorus calamus L.) effect on the experimental animals functions

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2010-6-18-23 ◽

2010 ◽

Vol 9 (6) ◽

pp. 18-23

Author(s):

A. M. Gurieyv ◽

M. V. Belousov ◽

R. R. Akhmedzhanov ◽

M. S. Yusubov ◽

A. A. Churin ◽

...

Keyword(s):

General Condition ◽

Functional Activity ◽

Water Soluble ◽

Acorus Calamus ◽

Pathological Changes ◽

Internal Organs ◽

Experimental Animals ◽

General Weight ◽

Intravenous Introduction

An investigation has been performed as to the effect of a long-term parenteral introduction of the sum of water-soluble polysaccharides (WSPS) calamus root (Acorus calamus L.) extracted by means of the in-house technology on the functions of experimental animals. As it follows from the results of the experiments, the intragastric introduction of the WSPS to rats in the doses of 20, 100, and 200 mg/kg during a three-month term and the intravenous introduction to rabbits in the doses of 25 and 50 mg/kg during a one-month term do not lead to the death of the animals or to any pathological changes in their general condition, the dynamics of the general weight, or the functional activity of the investigated internal organs and systems.

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OXIC-HYGIENIC STUDY OF THE USE OF A SYNBIOTIC SUSPENSION AND A PROBIOTIC MODIFIED GINGIVAL GEL ON ANIMALS

СИСТЕМНЫЙ АНАЛИЗ И УПРАВЛЕНИЕ В БИОМЕДИЦИНСКИХ СИСТЕМАХ ◽

10.36622/vstu.2021.20.1.006 ◽

2021 ◽

pp. 44-50

Author(s):

Наталия Владимировна Чиркова ◽

Алена Алексеевна Плутахина ◽

Ксения Евгеньевна Чиркова ◽

Елена Анатольевна Андреева ◽

Елена Юрьевна Каверина

Keyword(s):

High Frequency ◽

Antibacterial Agents ◽

Periodontal Diseases ◽

Age Groups ◽

Blood Parameters ◽

Internal Organs ◽

Toxicological Study ◽

Experimental Animals ◽

White Rats ◽

Weight Coefficients

Лечение заболеваний пародонта представляет собой одну из наиболее сложных и важных проблем современной стоматологии, актуальность которой обусловлена высокой частотой воспалительных заболеваний пародонта среди населения во всех возрастных группах - до 90% в популяции. Нерегулярность профилактики и эпизодические обращения к врачу - пародонтологу для лечения заболеваний пародонтита утяжеляют течение заболевания, которое при отсутствии лечения прогрессирует. Развитие пародонтита является результатом дисбаланса между микрофлорой полости рта и иммунной защитой организма. С целью ликвидации воспаления применяют различные иммунокорректоры и антибактериальные средства (антисептики, антибиотики, фитопрепараты). Альтернативой антибактериальной терапии при заболеваниях пародонта являются различные методы биотерапевтического воздействия, предполагающие местное и системное применение синбиотиков и пробиотиков, фаговых препаратов и других средств. Токсико - гигиенические исследования использования суспензии синбиотика «Бифистим», а также геля «Асепта с прополисом», модифицированного пробиотиком «Бифилиз» проводились на базе НИИ ВГМУ им. Н.Н. Бурденко. Была проведена оценка в остром и хроническом токсикологических экспериментах на 50 белых крысах (самцах) и на 5 морских свинках. Изучались кожно-резорбтивное действие, кумулятивные свойства, динамика массы и температуры тела, весовые коэффициенты внутренних органов, исследование показателей периферической крови. Оценка проведенных результатов токсикологического исследования опытных животных подтвердила отсутствие токсического действия исследуемой суспензии синбиотика «Бифистим», а также геля для десен «Асепта с прополисом», модифицированного пробиотиком «Бифилиз». Данные исследования позволили сделать вывод, что изучаемые препараты не токсичны и могут использоваться для лечения пациентов Treatment of periodontal diseases is one of the most difficult and important problems of modern dentistry, the relevance of which is due to the high frequency of inflammatory periodontal diseases among the population in all age groups - up to 90% in the population. Irregular prophylaxis and occasional visits to a periodontist for the treatment of periodontitis complicate the course of the disease, which progresses in the absence of treatment. The development of periodontitis is the result of an imbalance between the microflora of the oral cavity and the body's immune defenses. In order to eliminate inflammation, various immunocorrectors and antibacterial agents (antiseptics, antibiotics, phytopreparations) are used. An alternative to antibiotic therapy for periodontal diseases are various methods of biotherapeutic action, involving the local and systemic use of probiotics, phage preparations and other agents. Toxic and hygienic studies of the use of a suspension of sinbiotics "Bifistim" and as well as the gel "Asepta with propolis" modified with the probiotic "Bifiliz" were carried out on the basis of the Research Institute of VSMU them. N.N. Burdenko. Evaluation was carried out in acute and chronic toxicology experiments on 50 white rats (males) and 5 guinea pigs. Studied skin-resorptive effect, cumulative properties, dynamics of body weight and temperature, weight coefficients of internal organs, study of peripheral blood parameters. Evaluation of the results of the toxicological study of experimental animals confirmed the absence of the toxic effect of the investigated suspension of sinbiotics "Bifistim" as well as the gel for gums "Asepta with propolis", modified by the probiotic "Bifiliz". These studies led to the conclusion that the studied drugs are not toxic and can be used to treat patients

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