scholarly journals Fusion of purple membranes triggered by immobilization on carbon nanomembranes

2021 ◽  
Vol 12 ◽  
pp. 93-101
Author(s):  
René Riedel ◽  
Natalie Frese ◽  
Fang Yang ◽  
Martin Wortmann ◽  
Raphael Dalpke ◽  
...  
Keyword(s):  
Surface Coverage ◽  
Nitrilotriacetic Acid ◽  
Solid Support ◽  
Hybrid Membrane ◽  
Proton Pumping ◽  
Cross Linking ◽  
Two Dimensional ◽  
Self Assembled Monolayer ◽  
His Tag ◽  
Purple Membranes

A freestanding ultrathin hybrid membrane was synthesized comprising two functional layers, that is, first, a carbon nanomembrane (CNM) produced by electron irradiation-induced cross-linking of a self-assembled monolayer (SAM) of 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) and second, purple membrane (PM) containing genetically modified bacteriorhodopsin (BR) carrying a C-terminal His-tag. The NBPT-CNM was further modified to carry nitrilotriacetic acid (NTA) terminal groups for the interaction with the His-tagged PMs forming a quasi-monolayer of His-tagged PM on top of the CNM-NTA. The formation of the Ni-NTA/His-tag complex leads to the unidirectional orientation of PM on the CNM substrate. Electrophoretic sedimentation was employed to optimize the surface coverage and to close gaps between the PM patches. This procedure for the immobilization of oriented dense PM facilitates the spontaneous fusion of individual PM patches, forming larger membrane areas. This is, to our knowledge, the very first procedure described to induce the oriented fusion of PM on a solid support. The resulting hybrid membrane has a potential application as a light-driven two-dimensional proton-pumping membrane, for instance, for light-driven seawater desalination as envisioned soon after the discovery of PM.

scholarly journals Transformation Between 2D and 3D Covalent Organic Frameworks via Reversible [2+2] Cycloaddition

2020 ◽  
Author(s):  
Thaksen Jadhav ◽  
Yuan Fang ◽  
Cheng-Hao Liu ◽  
Afshin Dadvand ◽  
Ehsan Hamzehpoor ◽  
...  
Keyword(s):  
Band Structure ◽  
Absorption Edge ◽  
Room Temperature ◽  
Three Dimensional ◽  
Cross Linking ◽  
Two Dimensional ◽  
Covalent Organic Frameworks ◽  
Doping Behavior ◽  
2D Polymers ◽  
2D And 3D

We report the first transformation between crystalline vinylene-linked two-dimensional (2D) polymers and crystalline cyclobutane-linked three-dimensional (3D) polymers. Specifically, absorption-edge irradiation of the 2D poly(arylenevinylene) covalent organic frameworks (COFs) results in topological [2+2] cycloaddition cross-linking the π-stacked layers in 3D COFs. The reaction is reversible and heating to 200°C leads to a cycloreversion while retaining the COF crystallinity. The resulting difference in connectivity is manifested in the change of mechanical and electronic properties, including exfoliation, blue-shifted UV-Vis absorption, altered luminescence, modified band structure and different acid-doping behavior. The Li-impregnated 2D and 3D COFs show a significant ion conductivity of 1.8×10<sup>−4</sup> S/cm and 3.5×10<sup>−5</sup> S/cm, respectively. Even higher room temperature proton conductivity of 1.7×10<sup>-2</sup> S/cm and 2.2×10<sup>-3</sup> S/cm was found for H<sub>2</sub>SO<sub>4</sub>-treated 2D and 3D COFs, respectively.

Vapor Deposition of Composite Organic-Inorganic Films

2005 ◽  
Author(s):  
B. Kobrin ◽  
J. Chin ◽  
W. R. Ashurst
Keyword(s):  
Vapor Deposition ◽  
Room Temperature ◽  
Surface Coverage ◽  
Composite Coatings ◽  
Composite Films ◽  
Deposition Process ◽  
Deposition Method ◽  
Self Assembled Monolayer ◽  
Process Sequence ◽  
Inorganic Films

Results on the thermal and immersion stability of ultra-thin composite films created by a deposition method call MVD™ (Molecular Vapor Deposition [1]) are reported. It is observed that these composite films were denser and more stable in thermal and immersion applications when compared to traditional self-assembled monolayer (SAM) films. These improved films were created by a special “sequential” or “layered” deposition process sequence. The MVD™ composite coatings can be deposited at room temperature on a variety of materials such as polymers, fibers, metals, alloys and other materials which normally do not allow films to form with complete surface coverage.

Two-Dimensional Crystal Structure of a Quaterthiophene−Alkanethiol Self-Assembled Monolayer on Gold

Langmuir ◽  
2008 ◽  
Vol 24 (16) ◽  
pp. 8458-8464 ◽  
Author(s):  
C. Nogues ◽  
P. Lang ◽  
B. Desbat ◽  
T. Buffeteau ◽  
L. Leiserowitz
Keyword(s):  
Crystal Structure ◽  
Two Dimensional ◽  
Self Assembled Monolayer ◽  
Self Assembled ◽  
Dimensional Crystal

scholarly journals High surface coverage of a self-assembled monolayer by in situ synthesis of palladium nanodeposits

Nanoscale ◽  
2017 ◽  
Vol 9 (35) ◽  
pp. 13281-13290 ◽  
Author(s):  
Lucía Herrer ◽  
Victor Sebastian ◽  
Santiago Martín ◽  
Alejandro González-Orive ◽  
Francesc Pérez-Murano ◽  
...  
Keyword(s):  
Surface Coverage ◽  
High Surface ◽  
In Situ Synthesis ◽  
Self Assembled Monolayer ◽  
Confined Growth ◽  
Growth Method ◽  
Self Assembled

Nascent metal|monolayer|metal devices have been fabricated by depositing palladium, by growing palladium nanodeposits through a CO-confined growth method.

Morphology-Controlled 2D Ordered Microstructure Arrays by Surface Modification of Colloidal Template

2008 ◽  
Vol 8 (9) ◽  
pp. 4436-4440 ◽  
Author(s):  
Jian-Hong Lee ◽  
Ing-Chi Leu ◽  
Yi-Wen Chung ◽  
Min-Hsiung Hon
Keyword(s):  
Surface Characteristics ◽  
Two Dimensional ◽  
Polystyrene Spheres ◽  
Self Assembled Monolayer ◽  
Periodic Arrays ◽  
Colloidal Monolayer ◽  
Surface Modified ◽  
Modified Polystyrene ◽  
First Time ◽  
Structure Patterns

In this paper, we describe a convenient approach for fabricating two-dimensional, ordered TiO2 bowl-like structure and inverted-bowl-like structure patterns consisting of submicrometer arrays on a self-assembled monolayer film, for the first time using a surface-modified polystyrene colloidal monolayer as the template. Typically, the well-ordered two-dimensional TiO2 bowl-like structures were obtained by liquid phase deposition within the interstitial voids in assemblies of polystyrene spheres followed by the dissolution of the polystyrene template with dichloromethane. Otherwise, through a simple wet treatment by immersing in the sulfuric acid at 50 °C, the surface characteristics of polystyrene were turned from hydrophobic to hydrophilic. The ordered inverted-bowl-like structures could be prepared on this treated surface. We believe that this method could be extendable to colloidal template with smaller dimensions, and to periodic arrays made of other materials.

THE ELECTROCHEMICAL STUDIES ON A SELF-ASSEMBLED VIOLOGEN MONOLAYER USING QUARTZ CRYSTAL MICROBALANCE

2002 ◽  
Vol 01 (05n06) ◽  
pp. 593-596 ◽  
Author(s):  
J. Y. OCK ◽  
H. K. SHIN ◽  
Y. S. KWON ◽  
S. H. SONG ◽  
S. M. CHANG ◽  
...  
Keyword(s):  
Self Assembly ◽  
Quartz Crystal ◽  
Surface Coverage ◽  
Gold Surface ◽  
Frequency Change ◽  
Self Assembled Monolayer ◽  
Solution Interface ◽  
Reversible Redox ◽  
Mass Detector ◽  
Self Assembled

The electrochemical behavior of viologen self-assembled monolayer has been investigated with QCM, which has been known as ng order mass detector. The self-assembly process of viologen was monitored using resonant frequency (ΔF) and resonant resistance (R). The QCM measurements indicated a mass adsorption for viologen assembling on the gold surface with a frequency change of about 300, 135 Hz and its surface coverage (Γ) was calculated to be 5.02 × 10-9 and 1.64 × 10-9 mol/cm2. A reversible redox process was also observed and analyzed with an ionic interaction at the viologen/solution interface using ΔF.

scholarly journals Addition of Six-His-Tagged Peptide to the C Terminus of Adeno-Associated Virus VP3 Does Not Affect Viral Tropism or Production

2002 ◽  
Vol 76 (23) ◽  
pp. 12023-12031 ◽  
Author(s):  
Huang-Ge Zhang ◽  
Jinfu Xie ◽  
Igor Dmitriev ◽  
Elena Kashentseva ◽  
David T. Curiel ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Affinity Purification ◽  
High Titer ◽  
Plasmid Vector ◽  
Nitrilotriacetic Acid ◽  
Start Codon ◽  
Wild Type ◽  
Adeno Associated Virus ◽  
Viral Tropism ◽  
His Tag

ABSTRACT Production of large quantities of recombinant adeno-associated virus (AAV) is difficult and not cost-effective. To overcome this problem, we have explored the feasibility of creating a recombinant AAV encoding a 6×His tag on the VP3 capsid protein. We generated a plasmid vector containing a six-His (6×His)-tagged AAV VP3. A second plasmid vector was generated that contained the full-length AAV capsid capable of producing VP1 and VP2, but not VP3 due to a mutation at position 2809 that encodes the start codon for VP3. These plasmids, necessary for production of AAV, were transfected into 293 cells to generate a 6×His-tagged VP3mutant recombinant AAV. The 6×His-tagged VP3 did not affect the formation of AAV virus, and the physical properties of the 6×His-modified AAV were equivalent to those of wild-type particles. The 6×His-tagged AAV did not affect the production titer of recombinant AAV and could be used to purify the recombinant AAV using an Ni-nitrilotriacetic acid column. Addition of the 6×His tag did not alter the viral tropism compared to wild-type AAV. These observations demonstrate the feasibility of producing high-titer AAV containing a 6×His-tagged AAV VP3 capsid protein and to utilize the 6×His-tagged VP3 capsid to achieve high-affinity purification of this recombinant AAV.

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