Characterization of Seasonal and Chemotypical Variability in the Volatile Oil from Leaves of Annona neosalicifolia H.Rainer (Annonaceae)

Introduction: Centratherum anthelminticum is an ethnomedicinal plant in India and a common ingredient in Ayurvedic formulations. The pharmacological effects of C. anthelminticum ranges from anti-oxidant, anti-diabetic, anti-microbial to recently found anti-cancer activity. Citrus aurantifolia (Rutaceae) is mainly used in daily consumption, in many cultural cuisines, and in juice production. It is widely used because of its antibacterial, anticancer, antidiabetic, antifungal, anti-hypertensive, anti-inflammation, anti-lipidemia, and antioxidant properties. Objective: To isolate and identify the compounds in the essential oils from the seeds of C. anthelminticum Kuntz and peels of C. aurantifolia (Christm.) swingle collected from the Delhi NCR region. Materials and Methods: The essential oils from seeds of C. anthelminticum Kuntz and peels of C. aurantifolia were extracted by steam distillation and analyzed by gas chromatograph and gas chromatography–mass spectrometer. Results: Chemical characterization of the volatile oil and seeds of C. anthelminticum Kuntz showed that the oil is greenish and semi viscous. The major component of the oil was found to be beta-caryophyllene (64.43%), beta-pinene (9.81%), alpha-humulene (4.64%), caryophyllene oxide (2.24%), gamma-elemene (1.73%), limonene (1.48%) and myrecene (0.11%). Whereas the major constituents of volatile oil from peels of C. aurantifolia comprises of twenty nine components viz. sesquiterpne alcohols (20.68%) including Globulol, Hurmularel, 6 - dien - 3 - ol. (-) -Spathulenol, ledol,alpha-bisabolol, - (-) Spathulenol and Sesquíterpene esters (20.68%), caryophyllene, alpha- Farnesene, alpha-Caryophylline, gamma -elemene, gamma-.neoclovene, monoterpene alcohol (13.79%). Conclusion: The present study revealed the chemical composition of essential oils of both the drugs under study which can be used as references in the food, pharmaceutical and cosmetic industries.

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Complex characterization of native Anethum graveolens volatile oil

Journal of Biotechnology ◽

10.1016/j.jbiotec.2015.06.323 ◽

2015 ◽

Vol 208 ◽

pp. S103

Author(s):

Alina Ortan ◽

Alexandru Ioan Calin ◽

Cristina Dinu Pirvu

Keyword(s):

Volatile Oil ◽

Anethum Graveolens ◽

Complex Characterization

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Study Regarding the Production and Characterization of Rose Petal Jam Enriched with Saint John`s Wort (Hypericum Perforatum) Essential Oil

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Food Science and Technology ◽

10.15835/buasvmcn-fst:10018 ◽

2014 ◽

Vol 71 (1) ◽

pp. 11

Author(s):

Maria Doinita Bors ◽

Georgiana Smaranda Petrut ◽

Maria Tofana ◽

Sonia Socaci ◽

Anca Farcas

Keyword(s):

Essential Oil ◽

Vitamin C ◽

Antioxidant Capacity ◽

Dry Matter ◽

Volatile Oil ◽

Ash Content ◽

Total Sugar ◽

Rose Petal ◽

The Rose

The purpose of this study was to extract the volatile oil from St. John's wort and use it to obtain a new product with improved qualities and real health benefits. In order to characterize the innovative product, several physicochemical analyses were conducted (dry matter, ash content, total sugar, total acidity, vitamin C, flavonoid content and antioxidant capacity). Also the consumer perception was followed by conducting a sensory analysis. The study revealed that the adition of H. perforatum essential oil in the rose petal jam imporved the vitamin C and flavoinoid content and also the antioxidant capacity of the product, meanwhile the dry matter, ash content and total sugar determinations were normal for the free pectin jam category.

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Synthesis and Characterization of Jia-Xiang Chrysanthemum Volatile Oil/β-Cyclodextrin Inclusion Complex

Proceedings of the National Academy of Sciences India Section A Physical Sciences ◽

10.1007/s40010-014-0126-8 ◽

2014 ◽

Vol 84 (3) ◽

pp. 381-385 ◽

Cited By ~ 1

Author(s):

Dawei Qin ◽

Xia Meng ◽

Lei Li

Keyword(s):

Inclusion Complex ◽

Volatile Oil ◽

Synthesis And Characterization ◽

Cyclodextrin Inclusion ◽

Cyclodextrin Inclusion Complex

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Chemical Characterization of Basil (Ocimum spp.) Germplasm from Brazil

HortScience ◽

10.21273/hortsci.32.3.464b ◽

1997 ◽

Vol 32 (3) ◽

pp. 464B-464

Author(s):

Roberto F. Vieira ◽

James E. Simon

Keyword(s):

Natural Products ◽

Traditional Medicine ◽

Chemical Characterization ◽

Volatile Oil ◽

The Other ◽

Methyl Cinnamate ◽

Methyl Chavicol ◽

The One

Ocimum species are largely used in Brazil both as a condiment and in traditional medicine against bronchitis, cough, and sorethroat in the form of tea or syrup. As little research has examined the natural products from Brazilian basil, 14 accessions of Ocimum, including O. basilicum (4), O. campechianum (3), O. gratissimum (6), and O. kilimandsharicum (1), collected in Brazil were grown in the Purdue Univ. greenhouse and upon maturity harvested, the volatile oil extracted and analyzed by GC/MS. Thirty-one constituents were identified. Three accessions of O. gratissimum showed high content of eugenol (40% to 66%), while the other accessions contained either high thymol (33%) or p-cymene (28% to 42%). The constituents of the single O. kilimandscharicum included 1,8-cineole (39%), methyl-chavicol (21%), and ß-bisabolene (23%). O. campechianum accessions contained either high 1,8-cineole (62%) or high ß-caryophyllene (79%). O. basilicum could also be separated chemically: a linalool:methyl chavicol type (47:28%); one methyl chavicol type (72%), and a third, methyl cinnamate (61%). One accession was identified containing >90% trans-methyl cinnamate, which crystallized during extraction. Plants rich in targeted compounds, such as the one with 90% trans-methyl cinnamate, can be used as source of germplasm for breeding and potential commercialization

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ID:2024 Synthesis and Characterization of PLGA-PEG Thymoquinone Nanoparticle and its Cytotoxicity Effects in Tamoxifen-resistant Breast Cancer Cells

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.248 ◽

2017 ◽

Vol 4 (S) ◽

pp. 55 ◽

Cited By ~ 1

Author(s):

Rozaina Ahmad ◽

Noor Haida Mohd Kaus ◽

Shahrul Hamid

Keyword(s):

Breast Cancer ◽

Drug Release ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Spherical Shape ◽

Entrapment Efficiency ◽

Volatile Oil ◽

Pluronic F68

Background: Drug resistance has been a continuous challenge in cancer treatment. The use of nanotechnology in the development of new cancer drugs has potential. One of the extensively studied compound is thymoquinone (TQ) and this work aims to compare two types of TQ-nanoformulation and its cytotoxicity towards resistant cancer cells. Methods: TQ-nanoparticles were prepared and optimized by using 2 different formulation with different drug to PLGA-PEG ratio (1:20 and 1:7) and different PLGA-PEG to pluronic F68 ratio (10:1 and 2:1). The morphology and size were determined using TEM and DLS. Characterization of particles were done using UV-VIS, ATR-IR, entrapment efficiency and drug release. The effects of drug, polymer and surfactants were compared between the two formulations. Cytotoxicity assay was performed using MTS assay. Results: TEM finding showed 96% of particles produced with 1:7 drug to PLGA-PEG were less than 90 nm in size and spherical in shape. This was confirmed with DLS which showed smaller particle size than those formed with 1:20 drug to PLGA-PEG ration. Further analysis showed zeta potential was negatively charge which could facilitate cellular uptake as reported previously. In addition, PDI value was less than 0.1 in both formulations indicating monodispersed and less broad in size distribution. The absorption peak of PLGA-PEG-TQ-Nps were at 255 nm. The 1:7 drug to polymer formulation was selected for further analysis where the entrapment efficiency was 79.9% and in vitro drug release showed a maximum release of TQ at 64% respectively. Cytotoxicity result showed IC50 of TQ-nanoparticle at 20.05 µM and free TQ was 8.25 µM. Conclusion: This study showed that nanoparticle synthesized with 1:7 drug to PLGA-PEG ratio and 2:1 PLGA-PEG to pluronic F68 were less than 100 nm and had spherical shape as confirmed with DLS. This could facilitate its transportation and absorption to reach its target. There was conserved TQ stability as exhibited by slow release of this volatile oil. Cytotoxicity effect was noted when resistant breast cancer cells were treated with these particles.

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Characterization of Chemical Composition of Pericarpium Citri Reticulatae Volatile Oil by Comprehensive Two-Dimensional Gas Chromatography with High-Resolution Time-of-Flight Mass Spectrometry

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/237541 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

Kunming Qin ◽

Lijuan Zheng ◽

Hao Cai ◽

Gang Cao ◽

Yajing Lou ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

High Resolution ◽

Time Of Flight ◽

Volatile Oil ◽

Two Dimensional ◽

Resolution Time ◽

Flight Mass Spectrometry

Pericarpium Citri Reticulatae (Chenpi in Chinese) has been widely used as an herbal medicine in Korea, China, and Japan. Chenpi extracts are used to treat indigestion and inflammatory syndromes of the respiratory tract such as bronchitis and asthma. This thesis will analyze chemical compositions of Chenpi volatile oil, which was performed by comprehensive two-dimensional gas chromatography with high-resolution time-of-flight mass spectrometry (GC × GC-HR-TOFMS). One hundred and sixty-seven components were tentatively identified, and terpene compounds are the main components of Chenpi volatile oil, a significant larger number than in previous studies. The majority of the eluted compounds, which were identified, were well separated as a result of high-resolution capability of the GC × GC method, which significantly reduces, the coelution.β-Elemene is tentatively qualified by means of GC × GC in tandem with high-resolution TOFMS detection, which plays an important role in enhancing the effects of many anticancer drugs and in reducing the side effects of chemotherapy. This study suggests that GC × GC-HR-TOFMS is suitable for routine characterization of chemical composition of volatile oil in herbal medicines.

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