scholarly journals Optimization of NPK Medium with the Addition of Mung Bean Sprout Extract for Biomass and Lipid Production of Stanieria HS-48 as a Potential Biofuel

2021 ◽  
Vol 82 (1) ◽  
pp. 77-84
Author(s):  
Ni Wayan Kristina Eka Yanti ◽  
Aditya Toriq Rochmanto ◽  
Sri Handayani ◽  
Nasruddin ◽  
Nining Betawati Prihantini
Keyword(s):  
Growth Medium ◽  
Lipid Content ◽  
Mung Bean ◽  
Hot Spring ◽  
Lipid Production ◽  
Total Cell ◽  
Raw Material ◽  
Good Growth ◽  
Bean Sprout ◽  
Total Cell Density

Stanieria strain HS-48 from Ciater hot spring, Indonesia contains lipid content in the form of fatty acids that can be used as a raw material for biodiesel. Optimization of the growth medium was used to produce high contents of microalgal lipids. The growth medium in this study utilized NPK medium with the addition of variations in the concentration of mung bean sprout extract 2% and 4%. This study aimed to determine the best concentration of mung bean sprout extract in NPK medium for the highest biomass and lipid production of Stanieria HS-48. The experiments were conducted in airlift photobioreactors (APBR) for 19 days. The highest total cell density of Stanieria HS-48 in NPK medium with the addition of variations in the concentration of mung bean sprout extract 2% and 4% were 6.165 x 106 and 4.702 x 106 cell/ml. The lipid content of the biomass from Stanieria HS-48 on NPK medium with the addition of mung bean sprout extract 2% and 4% were 62.9% and 44.8%. The study showed that the NPK medium with the addition of the concentration of mung bean sprout extract 2% was a good growth medium for increasing biomass and lipid of Stanieria HS-48.

scholarly journals Use of Mung Bean Sprout (Tauge) as Alternative Fungal Growth Medium

2019 ◽  
Vol 1241 ◽  
pp. 012015
Author(s):  
M Ilmi ◽  
L K Putri ◽  
A A K Muhamad ◽  
A Cholishoh ◽  
S A Ardiansyah
Keyword(s):  
Growth Medium ◽  
Mung Bean ◽  
Fungal Growth ◽  
Bean Sprout

scholarly journals Requirement of Cyanobacteria Synechococcus elongatus for the biomass and lipid production

2021 ◽  
Vol 869 (1) ◽  
pp. 012042
Author(s):  
R Rusydi ◽  
A Yakupitiyage ◽  
S Pumpuang ◽  
S Mellisa ◽  
A Rahma ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Lipid Content ◽  
Specific Growth ◽  
Lipid Production ◽  
Optimal Dose ◽  
Synechococcus Elongatus ◽  
Fast Growth ◽  
Good Growth ◽  
Institute Of Technology

Abstract Synechococcus elongatus is unicellularCyanobacteria having fast growth and can conduct photosynthesis process in its cells.This research purposed to evaluate the potential of Synechococcus elongatus as an candidate of biodiesel feedstocks by evaluating its biomass and lipid content in appropriate nutrients. The study was conducted at Asian Institute of Technology, Thailand. The Factorial Completely Randomized Design was used as experimental design in this study. The species was grown in BG II medium which was added by different doses of NaNO3 and KHPO4. Cultivation was conducted for seven days for several parameters, namely appropriate dose of nutrients, specific growth rate, biomass, and lipid content. This study resulted the optimal dose of nitrate from NaNO3 and phosphate from KHPO4 for good growth of Synechococcus elongatus which were 289.11 mg/L of NO3- and 22.26 mg/L of HPO4.This species grew well in BG-II medium added optimal nutrients at specific growth rate of 0.34µg/day. The optimal lipid productivity of Synechococcus elongatus was achieved at day 6th of cultivation which its dried biomass was 0.21±0.03 g/L and total lipid was 1.89±0.28%. Based on the results, Synechococcus elongatus cultivated in BG-II medium was potential tobe biodiesel source with its fast growth and lipid content in its cell.

Effect of short-term growth of mung bean and its soil incorporation on the density of the soybean cyst nematode, Heterodera glycines, in pot experiments

Nematology ◽  
2017 ◽  
Vol 19 (10) ◽  
pp. 1147-1155 ◽  
Author(s):  
Seiya Chikamatsu ◽  
Wang Xiaoman ◽  
Daisuke Ito ◽  
Emi Yamada ◽  
Koki Toyota
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Raw Material ◽  
Short Term ◽  
Bean Sprout ◽  
Dry Soil ◽  
Moisture Conditions

We previously reported that incorporation of bean sprout residue into soil stimulated hatching of the soybean cyst nematode (SCN), Heterodera glycines. The objective of this study was to evaluate the effects of short-term growth of mung bean (Vigna radiata), the raw material of bean sprout, and its incorporation into the soil on the density of SCN in soil. When mung bean was grown in pots for 2 and 4 weeks in a SCN-infested andosol, incorporated into the soil and further incubated for 2 weeks, the SCN density decreased markedly. Mung bean was also grown in pots under different moisture conditions. The SCN density decreased in the soils with 48-68 g water, but not with 39 g water (100 g dry soil)−1 in which mung bean grew the poorest. The present study suggests that short-term growth of mung bean may be useful to decrease the density of SCN in soil.

scholarly journals Production of Lipids from Municipal Sewage Sludge by Two Stage Extraction Process

2016 ◽  
Vol 16 (1) ◽  
pp. 38
Author(s):  
Bharathi P. ◽  
Pennarsi M.
Keyword(s):  
Sewage Sludge ◽  
Lipid Content ◽  
Lipid Production ◽  
Extraction Process ◽  
Raw Material ◽  
Municipal Sewage ◽  
Municipal Sewage Sludge ◽  
Two Stage ◽  
Extraction Processes ◽  
Tlc Analysis

In this investigation, municipal sewage sludge was used as a lipid feedstock. Two stage extraction processes was developed to obtain good yield of lipid content. The maximum lipid yields 32.5% was achieved from chloroform: methanol solvents through optimum conditions of 2:1 ratio, 50oC temp for 30 min. The lipid content was characterized by TLC analysis. The lipid properties were analyzed and proved as a lipid. Hence, municipal sewage sludge serves as a valuable raw material for lipid production

scholarly journals miR-15b negatively correlates with lipid metabolism in mammary epithelial cells

2018 ◽  
Vol 314 (1) ◽  
pp. C43-C52 ◽  
Author(s):  
Meiqiang Chu ◽  
Yong Zhao ◽  
Shuai Yu ◽  
Yanan Hao ◽  
Pengfei Zhang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Epithelial Cells ◽  
Steroid Hormones ◽  
Lipid Content ◽  
Mammary Epithelial Cells ◽  
Lipid Production ◽  
Fatty Acid Synthetase ◽  
Lipid Synthesis ◽  
Mammary Epithelial ◽  
Lipid Formation

Mammary epithelial cells are regulated by steroid hormones, growth factors, and even microRNAs. miR-15b has been found to regulate lipid metabolism in adipocytes; however, its effects on lipid metabolism in mammary epithelial cells, the cells of lipid synthesis and secretion, are as yet unknown. The main purpose of this investigation was to explore the effect of miR-15b on lipid metabolism in mammary epithelial cells, along with the underlying mechanisms. miR-15b was overexpressed or inhibited by miRNA mimics or inhibitors; subsequently, lipid formation in mammary epithelial cells, and proteins related to lipid metabolism, were investigated. Through overexpression or inhibition of miR-15b expression, the current investigation found that miR-15b downregulates lipid metabolism in mammary epithelial cells and is expressed differentially at various stages of mouse and goat mammary gland development. Inhibition of miR-15b expression increased lipid content in mammary epithelial cells through elevation of the lipid synthesis enzyme fatty acid synthetase (FASN), and overexpression of miR-15b reduced lipid content in mammary epithelial cells with decreasing levels of FASN. Moreover, the steroid hormones estradiol and progesterone decreased miR-15b expression with a subsequent increase in lipid formation in mammary epithelial cells. The expression of miR-15b was lower during lactation and negatively correlated with lipid synthesis proteins, which suggests that it may be involved in lipid synthesis and milk production. miR-15b might be a useful target for altering lipid production and milk yield.

scholarly journals The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 463 ◽  
Author(s):  
Ali Nawaz Kumbhar ◽  
Meilin He ◽  
Abdul Razzaque Rajper ◽  
Khalil Ahmed Memon ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Lipid Content ◽  
Fossil Fuels ◽  
Fossil Fuel ◽  
Lipid Production ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Biofuel Production ◽  
Promising Strategy ◽  
Waste Extract

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.

scholarly journals PENGARUH PERBEDAAN UMUR PANEN TERHADAP KANDUNGAN LEMAK Nitzschia sp.

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Baiq Tri Khairina Ilhami, Lalu Japa Sri Puji Astuti dan Rina Kurnianingsih
Keyword(s):  
Lipid Content ◽  
Light Energy ◽  
Raw Material ◽  
Biodiesel Production ◽  
Wide Area ◽  
Experiment Research ◽  
Soxhlet Method ◽  
Sun Light

ABSTRAK Mikroalga merupakan protista berklorofil yang memanfaatkan energi matahari dan CO2 dalam proses fotosintesis sehingga dalam biomassanya terkandung bahan-bahan seperti: lemak, protein, dan karbohidrat. Salah satu jenis mikroalga yang memiliki kandungan lemak adalah Nitzschia sp. Kelebihan mikroalga sebagai bahan baku biodiesel jika dibandingkan dengan jarak, biji bunga matahari, jagung, dan tumbuhan lainnya yaitu pertumbuhan mikroalga yang sangat cepat, tidak bersaing dengan produksi pangan, tidak membutuhkan lahan yang luas, dan ramah lingkungan. Oleh karena itu, perlu dilakukan penelitian untuk mengetahui kandungan lemak tertinggi pada Nitzschia sp. berdasarkan umur kultivasi. Dilakukan penelitian eksperimental kuantitatif menggunakan  Rancangan Acak Lengkap (RAL) dengan 5  kali panen dan 3 kali ulangan, percobaan dilakukan di Laboratorium Pakan Alami Balai Budidaya Laut (BBL) Lombok, Sekotong pada bulan Agustus sampai September 2014. Kadar lemak ditentukan dengan Metode Soxhlet. Hasil penelitian menunjukkan umur pemanenan hari ke-13 memiliki kandungan lemak tertinggi dengan persentase sebesar 1,84% sedangkan kandungan lemak terendah pada umur panen hari ke-11 dengan persentase sebesar 0,89%. Tinggi rendahnya kandungan lemak Nitzschia sp. dipengaruhi oleh nutrien, kepadatan sel dan faktor lingkungan media kultur.  ABSTRACT Photosynthetic Protist, microalgae can trap sun light energy and CO2 for photosynthesis process, and they produce lipid, protein, and carbohydrate. One species of  microalga that can produce lipid in high portion of their total dry wight is Nitzschia sp. The lipid can highly use as raw material for biodiesel production. The benefits of  microalga as source of biodiesel are they grow faster, they do not compete with other food soruces production, they do not need wide area, and they are invironmental friendly. A quantitative experiment research of rendom complete design was done in the Laboratorium Pakan Alami Balai Budidaya Laut (BBL) Lombok, Sekotong during the months of August to  September 2014. The research was conducted in five time harfesting of each in three repetation The lipid content was ditermined using Soxhlet method. The results showing that the highest lipid content (1.84%) of Nitzschia sp. occurred at the day of thirteen of culturing, and the lowest lipid content (0.89%) of Nitzschia sp. was in the day of eleven of culture. 

Optimization of lipid accumulation in Pleurastrum insigne for biodiesel production

2021 ◽  
Vol 16 (10) ◽  
pp. 144-155
Author(s):  
Van Lal Michael Chhandama ◽  
Belur Kumudini Satyan
Keyword(s):  
Lipid Content ◽  
Lipid Production ◽  
Statistical Design ◽  
High Growth ◽  
High Growth Rate ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Biodiesel Standards ◽  
High Lipid ◽  
Different Sources

Microalgae emerged as a competent feedstock for biodiesel production because of high growth rate and lipid content. This work focuses on isolation of novel microalgal strain from different sources of water for the production of biodiesel. The isolated microalgae, Pleurastrum insigne possessed high lipid content (~28 % dcw), further optimized to 57.06 % dcw using a statistical design (CCD) under Response Surface Methodology. Lipid production was optimized by nutrient (nitrogen and phosphorus) and pH stress. The different type of fatty acids present in the optimized lipid was also profiled using GCMS. Biodiesel yield was found to be 82.14 % of the total lipid and the fuel properties tested have met IS, ASTM and EN biodiesel standards.

scholarly journals Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ruiling Gao ◽  
Zifu Li ◽  
Xiaoqin Zhou ◽  
Wenjun Bao ◽  
Shikun Cheng ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Yarrowia Lipolytica ◽  
Butyric Acid ◽  
Lipid Content ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Initial Ph ◽  
Alkaline Conditions

Abstract Background Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) VFAs on these yeasts has impeded the production of high lipid yields and their large-scale application. Slightly acidic conditions have been commonly adopted because they have been considered favorable to oleaginous yeast cultivation. However, the acidic pH environment further aggravates this inhibition because VFAs appear largely in an undissociated form under this condition. Alkaline conditions likely alleviate the severe inhibition of high-content VFAs by significantly increasing the dissociation degree of VFAs. This hypothesis should be verified through a systematic research. Results The combined effects of high acetic acid concentrations and alkaline conditions on VFA utilization, cell growth, and lipid accumulation of Yarrowia lipolytica were systematically investigated through batch cultures of Y. lipolytica by using high concentrations (30–110 g/L) of acetic acid as a carbon source at an initial pH ranging from 6 to 10. An initial pH of 8 was determined as optimal. The highest biomass and lipid production (37.14 and 10.11 g/L) were obtained with 70 g/L acetic acid, whereas cultures with > 70 g/L acetic acid had decreased biomass and lipid yield due to excessive anion accumulation. Feasibilities on high-content propionic acid, butyric acid, and mixed VFAs were compared and evaluated. Results indicated that YX/S and YL/S of cultures on butyric acid (0.570, 0.144) were comparable with those on acetic acid (0.578, 0.160) under alkaline conditions. The performance on propionic acid was much inferior to that on other acids. Mixed VFAs were more beneficial to fast adaptation and lipid production than single types of VFA. Furthermore, cultures on food waste (FW) and fruit and vegetable waste (FVW) fermentate were carried out and lipid production was effectively improved under this alkaline condition. The highest biomass and lipid production on FW fermentate reached 14.65 g/L (YX/S: 0.414) and 3.20 g/L (YL/S: 0.091) with a lipid content of 21.86%, respectively. By comparison, the highest biomass and lipid production on FVW fermentate were 11.84 g/L (YX/S: 0.534) and 3.08 g/L (YL/S: 0.139), respectively, with a lipid content of 26.02%. Conclusions This study assumed and verified that alkaline conditions (optimal pH 8) could effectively alleviate the lethal effect of high-content VFA on Y. lipolytica and significantly improve biomass and lipid production. These results could provide a new cultivation strategy to achieve simple utilizations of high-content VFAs and increase lipid production. Feasibilities on FW and FVW-derived VFAs were evaluated, and meaningful information was provided for practical applications.

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