scholarly journals Caracterización del primer caso de infección humana por Ehrlichia canis en Panamá

2019 ◽  
Vol 38 (3) ◽  
Author(s):  
Sergio Eduardo Bermúdez Castillero ◽  
Carlos Daza ◽  
Julio Osorio ◽  
Amilcar Hurtado ◽  
José Suárez ◽  
...  
Keyword(s):  
16S Rrna ◽  
Influenza A ◽  
Rhipicephalus Sanguineus ◽  
Severe Case ◽  
Human Infection ◽  
Ehrlichia Canis ◽  
Moderate Intensity ◽  
Rrna Gene ◽  
Serological Tests ◽  
First Case

<p>[Characterization of the First Case of Human Infection by Ehrlichia canis in Panama]</p><p><strong>Resumen</strong></p><p>Ehrlichia canis fue considerada un patógeno exclusivo de caninos y tiene como su principal vector a garrapatas del complejo Rhipicephalus sanguineus. Diversos estudios de casos demuestran que también puede producir patología en humanos, de las que hasta el momento sólo se han asociado a infecciones asintomáticas y de leve a moderada intensidad. El objetivo de este manuscrito es describir la presentación clínica de un caso severo de ehrlichiosis en un joven inmunocompetente y presentar el diagnóstico diferencial de E. canis. La determinación del agente etiológico se realizó por medio de pruebas serológicas (IFI) y moleculares (gltA, ompA, 16S rARN, ECC, ECB, Dsb-330 y Dsb-728) a muestras de sangre del paciente. No se obtuvieron resultados para pruebas serológicas contra dengue, hantavirus, HIV, Leptospira, tampoco dio resultado el PCR de parainfluenza (1, 2, 3), adenovirus, influenza (A y B) y rinovirus. Los resultados para Rickettsia demostraron sero-reacción IgG, pero no IgM ni para los análisis moleculares. Secuencias de los segmentos amplificados mostraron un 99% de homología con E. canis para el gen 16S rARN y de 100% el gen dsb. Este es el primer caso confirmado y descrito de infección de E. canis en un paciente inmunocompetente humano en Panamá.<br /><br /><strong>Abstract</strong><br />Ehrlichia canis was considered an exclusive canine pathogen and its main vector is ticks of the Rhipicephalus sanguineus complex. Several case studies show that it can also cause pathology in humans, which until now have only been associated with asymptomatic infections and mild to moderate intensity. The aim of this paper is describe the clinical presentation of a severe case of ehrlichiosis, an immuno-competent young man. Present the differential diagnosis of Ehrlichia canis. The determination of the etiological agent, serological (IFA) and molecular tests (gltA, ompA, 16S rRNA, ECC, ECB, Dsb-330 and Dsb-728) were performed on patient blood samples. No results were obtained for serological tests against dengue, hantavirus, HIV, Leptospira, nor on PCR for parainfluenza (1, 2, 3), adenovirus, influenza (A and B), and rhinovirus. The results for Rickettsia demonstrated sero-reaction IgG, but not IgM nor for molecular analyzes. Sequences of the amplified segments showed 99% homology with E. canis for the 16S rRNA gene and 100% for the dsb gene. This is the first confirmed and described case of E. canis infection in a human immunocompetent patient in Panama.<br /><br /></p>

scholarly journals Seroprevalence and Genotypic Analysis of Ehrlichia canis Infection in Dogs and Humans in Cauca, Colombia

2021 ◽  
Author(s):  
Elkin Forero-Becerra ◽  
Jignesh Patel ◽  
Heidy-C Martínez-Diaz ◽  
Paola Betancourt-Ruiz ◽  
Efraín Benavides ◽  
...  
Keyword(s):  
16S Rrna ◽  
Amplicon Sequencing ◽  
Immunofluorescence Assay ◽  
Positive Sample ◽  
Human Infection ◽  
Ehrlichia Canis ◽  
Rrna Gene ◽  
Blood Samples ◽  
Genotypic Analysis ◽  
Pcr Targeting

Ehrlichia canis infections have been reported in humans in Venezuela and Costa Rica. In this study, 506 healthy residents and 114 dogs from four municipalities (Cauca, Colombia) were surveyed and blood samples collected. Antibodies to E. canis in human and canine sera were evaluated using the Tandem repeat protein 19 (TRP19) peptide ELISA and indirect immunofluorescence assay (IFA). Ehrlichia canis TRP19 antibodies were detected in only 1/506 human sera, but the single positive sample was negative by IFA. The majority (75/114; 66%) of dogs surveyed had antibodies to the E. canis TRP19 peptide by ELISA, and eight randomly selected sera were further confirmed by E. canis IFA. Genomic DNA samples obtained from 73 E. canis TRP19 ELISA–positive dog blood samples were examined by PCR targeting the 16S rRNA gene. Ehrlichia canis 16S rRNA was amplified in 30 (41%) of the dogs, and 16 amplicons were selected for DNA sequencing, which confirmed that all were E. canis. A second PCR was performed on the 16 confirmed E. canis 16S rRNA PCR–positive samples to determine the TRP36 genotype by amplifying the trp36 gene. TRP36 PCR amplicon sequencing identified nine dogs infected with the U.S. E. canis TRP36 genotype (56%), one dog with the Brazilian genotype (6%), and six dogs with the Costa Rican genotype (38%). Moreover, these molecular genotype signatures were consistent with serologic analysis using TRP36 genotype–specific peptides. Notably, there was no serologic evidence of E. canis infection in humans, suggesting that E. canis infection in dogs in Cauca is not associated with zoonotic human infection.

scholarly journals First case of an invasive Bacteroides dorei infection detected in a patient with a mycotic aortic aneurysm—raising a rebellion of major indigenous bacteria in humans: a case report and review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Takayuki Matsuoka ◽  
Takuya Shimizu ◽  
Tadanori Minagawa ◽  
Wakiko Hiranuma ◽  
Miki Takeda ◽  
...  
Keyword(s):  
Aortic Aneurysm ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Resected Specimen ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
First Case ◽  
Rrna Gene Sequencing

Abstract Background Bacteroides dorei is an anaerobic gram-negative bacterium first described in 2006. Because of the high similarity in mass spectra between B. dorei and Bacteroides vulgatus, discriminating between these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria, including B. dorei and B. vulgatus, at the genus and species levels. Consequently, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. However, there are no reports on invasive infectious diseases caused by B. dorei. This report describes the first case of direct invasion and colonisation of human tissue by B. dorei, thus providing a warning regarding the previously proposed application of B. dorei as a live biotherapeutic for atherosclerotic diseases. Case presentation A 78-year-old Japanese man complained of intermittent chest/back pain and was diagnosed with a mycotic thoracic aortic aneurysm by enhanced computed tomography on admission. Despite strict blood pressure control and empirical antibiotic therapy, the patient’s condition worsened. To prevent aneurysmal rupture and eliminate infectious foci, the patient underwent surgical treatment. The resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/B. vulgatus and B. dorei, respectively. The patient was successfully treated with appropriate antibacterial therapy and after improvement, was transferred to another hospital for rehabilitation on postoperative day 34. There was no recurrence of infection or aneurysm after the patient transfer. Conclusions This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.

scholarly journals Human infection with pandemic Influenza (H1N1): A Review Article

2019 ◽  
Vol 8 ◽  
pp. 191-199
Author(s):  
Ranjita Karmacharya
Keyword(s):  
Influenza A ◽  
H1n1 Influenza ◽  
Human Infection ◽  
Transmission Risk ◽  
Swine Flu ◽  
Scientific Databases ◽  
Health Authorities ◽  
First Case ◽  
Pandemic Influenza H1n1 ◽  
Awareness Programs

Swine flu, also known as the H1N1 virus, is new strain of an influenza virus that causes symptoms similar to the regular flu. It originated in pigs, but is spread rapidly from person to person. Therefore, the objectives of this article are to highlight on H1N1 Influenza, its mode of transmission, risk factor, clinical spectrums and preventive ways. Studies published relevant to swine flu was searched through scientific databases like MEDLINE, Pubmed and online search engines like ‘Google Scholar’. Eligible studies involved at least one factor related to ‘influenza A virus’, ‘H1N1 subtype’, ‘epidemiology’, ‘transmission” and ‘preventive measures’ etc. Searches were limited to human only and also looked at the websites of the leading health authorities (e.g. WHO, CDC, HPA). Swine is a new flu virus that appears in human and spreads very quickly from person to person. In Nepal, the first case was detected on June 2009 after that the case is increasing day by day. In 2010 till May there were 172 confirmed positive cases, likewise on August 7, 2017 there were 10 death and 354 positive cases of swine flu in Nepal. Therefore, Government of Nepal has broadcasted awareness programs, organized seminars and meetings, to make people aware regarding H1N1 Influenza. But, it seems challenging to decrease its outbreak in Nepal because of low financial resources, low manpower, limited technology and political instability.

scholarly journals Vogesella perlucida-induced bacteremia in an advanced-age patient: first case report

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Zengxian Yu ◽  
Fang Zhu ◽  
Xinghe Tao ◽  
Lu Zhang ◽  
Suliu Wu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Pathogenic Bacteria ◽  
Lower Limbs ◽  
Continuous Treatment ◽  
Advanced Age ◽  
Clinical Microbiology Laboratory ◽  
Rrna Gene ◽  
First Case ◽  
Microbiological Testing

Abstract Background Vogesella species are common aquatic, Gram-negative rod-shaped bacteria, originally described in 1997. Vogesella perlucida was first isolated from spring water in 2008. Furthermore, bacterial pathogenicity of Vogesella perlucida has never been reported. Here, we report the first case of rare Vogesella perlucida-induced bacteremia in an advanced-age patient with many basic diseases and history of dexamethasone abuse. Case presentation A 71-year-old female was admitted with inflamed upper and lower limbs, rubefaction, pain and fever (about 40 °C). She had been injured in a fall at a vegetable market and then touched river snails with her injury hands. A few days later, soft tissue infection of the patient developed and worsened. Non-pigmented colonies were isolated from blood cultures of the patient. Initially, Vogesella perlucida was wrongly identified as Sphingomonas paucimobilis by Vitek-2 system with GN card. Besides, we failed to obtain an acceptable identification by the MALDI-TOF analysis. Finally, the isolated strain was identified as Vogesella perlucida by 16S rRNA gene sequences. In addition, the patient recovered well after a continuous treatment of levofloxacin for 12 days. Conclusion Traditional microbiological testing system may be inadequate in the diagnosis of rare pathogenic bacteria. Applications of molecular diagnostics techniques have great advantages in clinical microbiology laboratory. By using 16S rRNA gene sequence analysis, we report the the first case of rare Vogesella perlucida-induced bacteremia.

scholarly journals Multiphasic Approach Reveals Genetic Diversity of Environmental and Patient Isolates of Mycobacterium mucogenicum and Mycobacterium phocaicum Associated with an Outbreak of Bacteremias at a Texas Hospital

2008 ◽  
Vol 74 (8) ◽  
pp. 2480-2487 ◽  
Author(s):  
Robert C. Cooksey ◽  
Michael A. Jhung ◽  
Mitchell A. Yakrus ◽  
W. Ray Butler ◽  
Toidi Adékambi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
16S Rrna ◽  
Restriction Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Bloodstream Infections ◽  
Clinical Isolates ◽  
Rrna Gene ◽  
First Case ◽  
Typing Methods ◽  
Mycobacterium Mucogenicum

ABSTRACT Between March and May 2006, a Texas hospital identified five Mycobacterium mucogenicum bloodstream infections among hospitalized oncology patients using fluorescence high-performance liquid chromatography analysis of mycolic acids. Isolates from blood cultures were compared to 16 isolates from environmental sites or water associated with this ward. These isolates were further characterized by hsp65, 16S rRNA, and rpoB gene sequencing, hsp65 PCR restriction analysis, and molecular typing methods, including repetitive element PCR, random amplified polymorphic DNA PCR, and pulsed-field gel electrophoresis (PFGE) of large restriction fragments. Three of five patient isolates were confirmed as M. mucogenicum and were in a single cluster as determined by all identification and typing methods. The remaining two patient isolates were identified as different strains of Mycobacterium phocaicum by rpoB sequence analysis. One of these matched an environmental isolate from a swab of a hand shower in the patient's room, while none of the clinical isolates of M. mucogenicum matched environmental strains. Among the other 15 environmental isolates, 11 were identified as M. mucogenicum and 4 as M. phocaicum strains, all of which were unrelated by typing methods. Although the 16S rRNA gene sequences matched for all 14 M. mucogenicum isolates, there were two each of the hsp65 and rpoB sequevars, seven PCR typing patterns, and 12 PFGE patterns. Among the seven M. phocaicum isolates were three 16S rRNA sequevars, two hsp65 sequevars, two rpoB sequevars, six PCR typing patterns, and six PFGE patterns. This outbreak represents the first case of catheter-associated bacteremia caused by M. phocaicum and the first report of clinical isolates from a U.S. hospital. The investigation highlights important differences in the available typing methods for mycobacteria and demonstrates the genetic diversity of these organisms even within narrow confines of time and space.

scholarly journals Rhodococcus erythropolis septicaemia in a patient with acute lymphocytic leukaemia

2011 ◽  
Vol 60 (2) ◽  
pp. 252-255 ◽  
Author(s):  
Soon Deok Park ◽  
Young Uh ◽  
In Ho Jang ◽  
Kap Jun Yoon ◽  
Hwang Min Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Lymphocytic Leukaemia ◽  
Rhodococcus Erythropolis ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Acute Lymphocytic Leukaemia ◽  
First Case ◽  
Rrna Gene Sequencing ◽  
Human Infections

Rhodococcus erythropolis rarely causes infection in humans. We report the second case of R. erythropolis septicaemia in a 7-year-old child. However, to our knowledge it is the first case in a patient with acute lymphocytic leukaemia who had been undergoing chemotherapy. The identification was performed using 16S rRNA gene sequencing. Even though R. erythropolis is rarely associated with human infections, it should be considered as a potential causative agent of bacteraemia, rather than overlooked as a contaminant.

scholarly journals Human infection with avian influenza A(H7N9) virus re-emerges in China in winter 2013

2013 ◽  
Vol 18 (43) ◽  
Author(s):  
E Chen ◽  
Y Chen ◽  
L Fu ◽  
Z Chen ◽  
Z Gong ◽  
...  
Keyword(s):  
Surveillance System ◽  
Influenza A ◽  
Severe Case ◽  
Clinical Findings ◽  
Human Infection ◽  
National Surveillance ◽  
H7n9 Virus ◽  
Laboratory Findings ◽  
Na Gene ◽  
National Surveillance System

Through a national surveillance system for unexplained pneumonia, a severe case of influenza A(H7N9) in a man in his mid-30s was identified in Zhejiang Province, China on 14 October 2013. Epidemiological and clinical findings were consistent with the patterns reported during the outbreak in spring 2013, and laboratory findings showed that the virus had 99.6% identity with earlier H7N9 viruses identified in humans in the spring except for five mutations in the NA gene.

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