scholarly journals The Effect of Infection by Toxocariasis on Some Biochemical Parameters in Some Areas of Salah Al-Din Governorate

2020 ◽  
Vol 1 (3) ◽  
pp. 89-92
Author(s):  
Enas Ermeith ◽  
Ashraf Jamal Mahmoud ◽  
Buthaina Jassim Yousef
Keyword(s):  
Fatty Acids ◽  
Low Density Lipoproteins ◽  
The Body ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Age Group ◽  
Very Low Density Lipoproteins ◽  
Large Numbers ◽  
Rate Of Increase ◽  
Group 15

The current study examined 277 blood samples of people, from both sexes of different ages, living in different areas of Salah Al-Din Governorate (Tikrit, Al-Alam and Baiji districts). The study was conducted from November, 2018 to June, 2019. All participants were secrened to investigate the incidence of toxocariasis using IgG TES-ELISA technique. The results showed that the rate of infection with toxocariasis was 22%. It was found an increase in cholesterol concentrations within age group 48-58 years and amounted to 185.8 mg/dl, and highest rate of increase in triglycerides was recorded within age group 15-25 years (168.1 mg/dl). The highest percentage of total protein was recorded within age group 26- 36 years (6.67 mg/dl). As for lipoprotein tests, highest percentage of high-density lipoproteins in age group 48-58 years was recorded (53 mg/dl), while low-density lipoproteins were highest in age group 48-58 years (100.8 mg/dl), and in very low-density lipoproteins the highest percentage within age group 15-25 years (33.5 mg/dl). The highest percentage of AST liver enzymes was recorded in age group 15-25 years and the percentage was 20.6 u/l. The highest percentage of ALT was recorded in age group 26-36 years (20.1 u/l) and 15-25 years (20 u/l). It is concluded that This age group continues to be exposed to infection with toxocariasis, which leads to presence large numbers of live larvae which secrete their antigens in the body and thus spread of toxoplasmosis, and the increase in proportion of fats is caused by poor oxidation of fatty acids and increased production of hepatic fatty acids Which leads to a delay in breakdown of lipoproteins, and the increase in level of AST, ALT enzyme occurs in cases of hepatitis and cirrhosis and may be associated with advanced cases of toxocariasis.

scholarly journals Stimulation of triacylglycerol synthesis in rat adipocytes by plasma very-low-density lipoproteins

1978 ◽  
Vol 176 (1) ◽  
pp. 169-174 ◽  
Author(s):  
P Thomopoulos ◽  
M Berthelier ◽  
D Lagrange ◽  
M J Chapman ◽  
M H Laudat
Keyword(s):  
Fatty Acids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Rat Adipocytes ◽  
Triacylglycerol Synthesis

The effect of human plasma lipoproteins on lipogenesis from glucose has been studied in isolated rat adipocytes. The very-low-density lipoproteins increased lipogenesis specifically, whereas low-density lipoproteins and high-density lipoproteins were without effect. Such stimulation could be reproduced with partially delipidated very-low-density lipoproteins. Nod-esterified fatty acids and glycerol were also without effect. Pretreatment of the adipocytes with trypsin did not alter the effect of very-low-density lipoprotein. The presence of Ca2+ was required for the full activation of lipogenesis. The synthesis of acylglycerol fatty acids and of acylglycerol glycerol were equally increased. The effect of very-low-density lipoprotein was not additive to that of insulin. It is suggested that very-low-density lipoprotein may directly stimulate lipogenesis in fat-cells, particularly in states when the lipoproteins are present at high concentration in the circulation.

Insufficient accuracy and specificity of polyanion precipitation methods for quantifying low-density lipoproteins

1990 ◽  
Vol 36 (12) ◽  
pp. 2109-2113 ◽  
Author(s):  
R Siekmeier ◽  
W März ◽  
W Gross
Keyword(s):  
Fatty Acids ◽  
Dextran Sulfate ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Nonesterified Fatty Acids ◽  
High Concentrations

Abstract Recently, polyanion precipitation assays for low-density lipoprotein (LDL)-cholesterol have been found to underestimate their analyte in normolipidemic samples (Siekmeier et al., Clin Chim Acta 1988;177:221-30). Therefore, accuracy, specificity, and interference by nonesterified fatty acids have been studied for three precipitants (obtained by heparin, dextran sulfate, or polyvinyl sulfate precipitation). At normal concentrations of LDL, precipitation is incomplete, whereas it is nearly quantitative at high concentrations of LDL. The polyvinyl sulfate reagent markedly responds to variations in the amount of non-LDL protein present in the precipitation mixture. In the dextran sulfate and the polyvinyl sulfate method, but not in the heparin method, the percentages of LDL precipitated notably increase as the concentration of the polyanion compound is decreased. In either assay, very-low-density lipoproteins, but not high-density lipoproteins, are significantly coprecipitated (dextran sulfate 28%, polyvinyl sulfate and heparin 66%) in a concentration-independent fashion. Increased concentrations of nonesterified fatty acids markedly interfere with the dextran sulfate and polyvinyl sulfate assay, but do not much affect results with the heparin reagent.

Metabolism of palmitate and linoleate in intact dogs

1962 ◽  
Vol 203 (5) ◽  
pp. 914-918 ◽  
Author(s):  
P. J. Nestel ◽  
A. Bezman ◽  
R. J. Havel
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Milk Fat ◽  
Plasma Lipids ◽  
Corn Oil ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Two Populations

Palmitate-9, 10-H3 and linoleate-1-C14 were infused simultaneously into intact dogs as albumin-bound free fatty acids or as esterified fatty acids in chylomicrons. Linoleate bound to albumin was removed more rapidly from the plasma and was incorporated more extensively into triglycerides of very low density lipoproteins of plasma than palmitate. Both linoleate and palmitate were incorporated more slowly into triglyceride fatty acids of low density and high density lipoproteins than into those of very low density lipoproteins. When chylomicrons obtained separately from dogs fed palmitate-9, 10-H3 with milk fat and linoleate-1-C14 with corn oil were mixed and infused into recipients, the rates of removal from the blood of both triglycerides and phospholipids of the two populations of chylomicrons differed, but this did not seem to depend on their chemical composition. Linoleate derived from chylomicron triglycerides disappeared from hepatic triglycerides and phospholipids more rapidly than palmitate and was incorporated more extensively into plasma lipids. Palmitate and linoleate were incorporated in equivalent amounts into circulating free fatty acids, but more linoleate was found in lipids of adipose tissue.

scholarly journals EVALUATION OF LIPID PROFILE COMPONENTS IN FOREIGN STUDENTS STUDYING AT THE IRKUTSK STATE MEDICAL UNIVERSITY

2018 ◽  
Vol 3 (5) ◽  
pp. 22-26
Author(s):  
M. A. Darenskaya ◽  
Ya. L. Danchinova ◽  
Adjitha Aaromal ◽  
L. I. Grebenkina ◽  
S. G. Aleksandrov ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Foreign Students ◽  
Low Density Lipoproteins ◽  
The Body ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Full Time ◽  
Very Low Density Lipoproteins ◽  
Functional Changes ◽  
Medical University

Background. A socially significant order for the training of foreign specialists, including the promotion of Russian education on the international market, certainly requires the organization of the process of students’ adaptation to the educational and information environment of the university. Particular attention deserves the components of the lipid profile, which are part of the overall adaptation syndrome.Aim: to study the features of the lipid profile in foreign students studying at the Irkutsk State Medical University.Methods. In total, 100 students of the medical profile of the Irkutsk State Medical University were enrolled in full-time study: 58 students (36 males and 22 females) were students of Russian nationality and 42 students (32 males and 11 females) – Indian students. Levels of lipid status indicators were determined with the help of Cormay kits on the automatic analyzer BTS-330 using the photometry method.Results. Hindu young men had statistically significantly higher values of triacylglycerols, very low-density lipoproteins and lower levels of high-density lipoproteins in comparison with young Russians. In the groups of girls of Indian origin, the changes concerned increased levels of triacylglycerols and very low-density lipoproteins in comparison with Russians. Gender differences were found only among Russian students – in the form of increased values of cholesterol and low-density lipoproteins in the group of girls compared to boys.Conclusion. The established functional changes in lipid metabolism in foreign students characterize the stress of the adaptive resources of the body, which, probably, can be due to the irrationality of nutrition.

scholarly journals Sirtuin 3 Restores Synthesis and Secretion of Very Low-Density Lipoproteins in Cow Hepatocytes Challenged with Nonesterified Fatty Acids In Vitro

2021 ◽  
Vol 8 (7) ◽  
pp. 121
Author(s):  
Dongmei Xing ◽  
Baogen Wang ◽  
Hong Lu ◽  
Tao Peng ◽  
Jianming Su ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Low Density Lipoproteins ◽  
Mrna Abundance ◽  
Low Density ◽  
Sirtuin 3 ◽  
Very Low Density Lipoproteins ◽  
Nonesterified Fatty Acids ◽  
Tag Accumulation

Fatty liver is closely associated with elevated concentrations of nonesterified fatty acids (NEFA) and a low level of very low-density lipoproteins (VLDL) in blood of dairy cows. High NEFA inhibit the VLDL synthesis and assembly, and cause hepatic triacylglycerol (TAG) deposition. Sirtuin 3 (SIRT3), a mitochondrial deacetylase, antagonizes NEFA-induced TAG accumulation through modulating expressions of fatty acid synthesis and oxidation genes in cow hepatocytes. However, the role of SIRT3 in the VLDL synthesis and assembly was largely unknown. Here we aimed to test whether SIRT3 would recover the synthesis and assembly of VLDL in cow hepatocytes induced by high NEFA. Primary cow hepatocytes were isolated from 3 Holstein cows. Hepatocytes were infected with SIRT3 overexpression adenovirus (Ad-SIRT3), SIRT3-short interfering (si) RNA, or first infected with Ad-SIRT3 and then incubated with 1.0 mM NEFA (Ad-SIRT3 + NEFA). Expressions of key genes in VLDL synthesis and the VLDL contents in cell culture supernatants were measured. SIRT3 overexpression significantly increased the mRNA abundance of microsomal triglyceride transfer protein (MTP), apolipoprotein B100 (ApoB100) and ApoE (p < 0.01), and raised VLDL contents in the supernatants (p < 0.01). However, SIRT3 silencing displayed a reverse effect in comparison to SIRT3 overexpression. Compared with NEFA treatment alone, the Ad-SIRT3 + NEFA significantly upregulated the mRNA abundance of MTP, ApoB100 and ApoE (p < 0.01), and increased VLDL contents in the supernatants (p < 0.01). Our data demonstrated that SIRT3 restored the synthesis and assembly of VLDL in cow hepatocytes challenged with NEFA, providing an in vitro basis for further investigations testing its feasibility against hepatic TAG accumulation in dairy cows during the perinatal period.

Selective uptake of cholesteryl esters from various classes of lipoproteins by HepG2 cells

1999 ◽  
Vol 77 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Louise Brissette ◽  
Marie-Claude Charest ◽  
Louise Falstrault ◽  
Julie Lafond ◽  
David Rhainds ◽  
...  
Keyword(s):  
Total Lipid ◽  
Hepg2 Cells ◽  
Ldl Receptor ◽  
Inverse Correlation ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Cholesteryl Esters ◽  
Very Low Density Lipoproteins ◽  
Selective Uptake

Selective uptake of cholesteryl esters (CE) from lipoproteins by cells has been extensively studied with high density lipoproteins (HDL). It is only recently that such a mechanism has been attributed to intermediate and low density lipoproteins (IDL and LDL). Here, we compare the association of proteins and CE from very low density lipoproteins (VLDL), IDL, LDL and HDL3 to HepG2 cells. These lipoproteins were either labelled in proteins with 125I or in CE with 3H-cholesteryl oleate. We show that, at any lipoprotein concentration, protein association to the cells is significantly smaller for IDL, LDL, and HDL3 than CE association, but not for VLDL. At a concentration of 20 µg lipoprotein/mL, these associations reveal CE-selective uptake in the order of 2-, 4-, and 11-fold for IDL, LDL, and HDL3, respectively. These studies reveal that LDL and HDL3 are good selective donors of CE to HepG2 cells, while IDL is a poor donor and VLDL is not a donor. A significant inverse correlation (r2 = 0.973) was found between the total lipid/protein ratios of the four classes of lipoproteins and the extent of CE-selective uptake by HepG2 cells. The fate of 3H-CE of the two best CE donors (LDL and HDL3) was followed in HepG2 cells after 3 h of incubation. Cells were shown to hydrolyze approximately 25% of the 3H-CE of both lipoproteins. However, when the cells were treated with 100 µM of chloroquine, a lysosomotropic agent, 85 and 40% of 3H-CE hydrolysis was lost for LDL and HDL3, respectively. The fate of LDL and HDL3-CE in HepG2 cells deficient in LDL-receptor was found to be the same, indicating that the portion of CE hydrolysis sensitive to chloroquine is not significantly linked to LDL-receptor activity. Thus, in HepG2 cells, the magnitude of CE-selective uptake is inversely correlated with the total lipid/protein ratios of the lipoproteins and CE-selective uptake from the two best CE donors (LDL and HDL3) appears to follow different pathways.Key words: lipoprotein, receptor, HepG2 cell, selective uptake, lipid, cholesterol, binding.

Comparison of the effects of escitalopram and atorvastatin on diet-induced atherosclerosis in rats

2014 ◽  
Vol 92 (3) ◽  
pp. 226-233 ◽  
Author(s):  
Amina Unis ◽  
Amany Abdelbary ◽  
Manal Hamza
Keyword(s):  
Histopathological Examination ◽  
Serum Levels ◽  
The Elderly ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Model Group ◽  
Very Low Density Lipoproteins ◽  
Atorvastatin Group ◽  
Atherosclerotic Changes

Atherosclerosis is one of the most common disorders among the elderly. Depression may be associated with the development of atherosclerosis. Thus, the aim of this study is to evaluate and compare the effects of escitalopram (a selective serotonin reuptake inhibitor) with atorvastatin (a well known antihyperlipidemic drug) on high fat diet induced atherosclerosis in rats. The results of this study showed that the administration of either escitalopram or atorvastatin for 6 weeks was associated with a significant decrease in serum levels of total cholesterol, triglycerides, low density lipoproteins, very low density lipoproteins, and serum malondialdehyde, and a significant increase in high density lipoproteins when compared with the atherosclerosis model group. Histopathological examination of the aortas from the test rats revealed significant regression of atherosclerotic changes, together with a significant decrease in vascular cell adhesion molecule-1 (VCAM-1) expression in the media of both the escitalopram group and the atorvastatin group when compared with the atherosclerosis model group. This study has shown that escitalopram reduced atherosclerotic changes, thus its use as an antidepressant in elderly patients should be considered.

scholarly journals Maternal loading with very low-density lipoproteins stimulates fetal surfactant synthesis

2002 ◽  
Vol 283 (2) ◽  
pp. L310-L318 ◽  
Author(s):  
Alan J. Ryan ◽  
Jheem D. Medh ◽  
Diann M. McCoy ◽  
Ronald G. Salome ◽  
Rama K. Mallampalli
Keyword(s):  
Fatty Acids ◽  
Knockout Mice ◽  
Low Density Lipoproteins ◽  
Alveolar Epithelial Cells ◽  
Type Ii Cells ◽  
Low Density ◽  
Type Ii ◽  
Very Low Density Lipoproteins ◽  
Pregnant Rat ◽  
Apolipoprotein E Knockout Mice

We examined whether administration of very low-density lipoproteins (VLDL) to pregnant rats increases surfactant phosphatidylcholine (PtdCho) content in fetal pre-type II alveolar epithelial cells. VLDL-triglycerides are hydrolyzed to fatty acids by lipoprotein lipase (LPL), an enzyme activated by heparin. Fatty acids released by LPL can incorporate into the PtdCho molecule or activate the key biosynthetic enzyme cytidylyltransferase (CCT). Dams were given BSA, heparin, VLDL, or VLDL with heparin intravenously. Radiolabeled VLDL given to the pregnant rat crossed the placenta and was distributed systemically in the fetus and incorporated into disaturated PtdCho (DSPtdCho) in pre-type II cells. Maternal administration of VLDL with heparin increased DSPtdCho content in cells by 45% compared with control ( P < 0.05). VLDL produced a dose-dependent, saturable, and selective increase in CCT activity. VLDL did not significantly alter immunoreactive CCT content but increased palmitic, stearic, and oleic acids in pre-type II cells. Furthermore, hypertriglyceridemic apolipoprotein E knockout mice contained significantly greater levels of DSPtdCho content in alveolar lavage and CCT activity compared with either LDL receptor knockout mice or wild-type controls that have normal serum triglycerides. Thus the nutritional or genetic modulation of serum VLDL-triglycerides provides specific fatty acids that stimulate PtdCho synthesis and CCT activity thereby increasing surfactant content.

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