Identification of Mentha piperita L. and Ricinus communis L. polyphe-nols by HPLC-DAD-ESI-MS and evaluation of their insecticidal properties against Aphis spiraecola P

2017 ◽  
Vol 7 (1) ◽  
pp. 28-34
Author(s):  
Malika Malika Boualem ◽  
Meriem Mokhtar ◽  
Farida Saiah ◽  
Fouzia Benourad ◽  
Radia Bouhadiba ◽  
...  
Keyword(s):  
Ricinus Communis ◽  
Syringic Acid ◽  
Mentha Piperita ◽  
Ionization Mass ◽  
Coumaric Acid ◽  
Esi Ms ◽  
Aphis Spiraecola ◽  
Ricinus Communis L ◽  
Array Detection ◽  
Dose Dependent

Aphids infest most crops, and are considered as one of the most destructive groups of insects in temperate regions. The purpose of this study was to identify the polyphenols of castor (Ricinus communis L.) and mint (Mentha piperita L.) with liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS), and evaluate their insecticidal properties against Aphis spiraecola P. A total of 10 compounds were identified in castor mainly phenolic acids (gallic acid, protocatechuic acid, syringic acid, chlorogenic acid, gentisic acid and p-coumaric acid). On the other hands, flavonoids (catechin, naringin, diosmin, rutin, diosmetin, luteolin, neohesperidin, naringenin and apigenin) were the major polyphe-nols in mint representing 10 compounds out of 14. In the test of insecticidal activity, four concentrations were tested (5, 10, 20 and 40%). Both plants had a good inhibitory activity especially mint. The effect was dose dependent and the highest mortality was obtained with the maximum used concentration (40%). After the first day, a percentage of 44 and 76% of mortality was recorded in the presence of just 5% of castor and mint which increased to reach 100% after 7 days.

scholarly journals ECDYSTEROIDS OF SILENE ITALICA: GLYCOSIDIC AND NONGLYCOSIDIC COMPONENTS AND HPLC-DAD-ESI-MS PROFILE

2019 ◽  
pp. 135-147
Author(s):  
Daniil Nikolayevich Olennikov ◽  
Nina Igorevna Kashchenko
Keyword(s):  
High Performance ◽  
Diode Array ◽  
Ionization Mass ◽  
Diode Array Detection ◽  
Mass Spectrometry Detection ◽  
Plant Weight ◽  
Esi Ms ◽  
Array Detection ◽  
First Time ◽  
Derivatives Of

  Silene italica (L.) Pers. is a species of Caryophyllaceae family which introduced samples contain ecdysteroids that was previously shown (Meng et al., 2001). In this paper, the composition of ecdysteroids of wild-growing S. italica was shown. Using high performance chromatography with diode-array detection and electrospray ionization mass-spectrometry detection (HPLC-DAD-ESI-MS) twenty-two compounds assigned to the ecdysteroid group. The non-glycoside components of S. italica were included twelve compounds and integristerone A, 26-hydroxyintegristerone A, 22-deoxyintegristerone A, 2-deoxyintegristerone A, and 2-deoxypolypodine B were identified for the first time. Ten compounds were characterized as ecdysteroid monoglycosides containing hexose fragment as a carbohydrate part. Trer were derivatives of 20-hydroxyecdysone, polypodine B, ecdysone and 2-deoxypolypodine B. For the first time, the presence of 22-deoxyintegristerone A hexoside non-detected in plant objects was found. Comparative analysis of the ecdysteroids composition of S. italica and well studied species S. italica spp. nemoralis (Báthori et al., 2000, 2002, 2004; Pongrácz et al., 2003; Simon et al., 2002) indicates their proximity. Quantitative analysis of five ecdysteroid content in the organs of S. italica showed that they were unevenly distributed in the plant. The concentration of 20-hydroxyecdysone was 0.10–32.12 mg/g and the total ecdysteroids content was 0.10–40.92 mg/g of dry plant weight. In general, it should be noted that the ability to produce and accumulate ecdysteroids was also observed for the wild samples of S. italica, which cultural samples was noted as ecdysteroid concentrator.

scholarly journals The chemical composition of the floral extract of Epipogium aphyllum sw. (Orchidaceae): A clue for their pollination biology

2014 ◽  
Vol 66 (3) ◽  
pp. 989-998 ◽  
Author(s):  
Anna Jakubska-Busse ◽  
Izabela Jasicka-Misiak ◽  
Anna Poliwoda ◽  
Emilia Święczkowska ◽  
Paweł Kafarski
Keyword(s):  
Mass Spectrometry ◽  
Chemical Composition ◽  
High Performance ◽  
Floral Scent ◽  
Pollination Biology ◽  
Ionization Mass ◽  
Methyl Cinnamate ◽  
Esi Ms ◽  
Array Detection ◽  
Pollination Systems

Epipogium aphyllum is a rare European obligate mycoheterotrophic orchid lacking chlorophyll. It has not been studied previously with respect to pollination biology. We studied the association between the composition of floral scent emission and its pollination systems. Field observation indicates that the main pollinators of Epipogium aphyllum are representatives of the genus Bombus (Hymenoptera), B. lucorum, B. hortorum, B. terrestris, B. pascuorum and B. proteus, and the genus Apis (Hymenoptera) namely A. mellifera. The main potential vector (observed to accidentally carry pollen), is most likely Episyrphus balteatus (Diptera, Syrphidae). The chemical composition of the floral extracts of 4 populations of Epipogium aphyllum Sw. growing naturally in Poland and the Czech Republic was examined by gas chromatography coupled with mass spectrometry (GC-MS) and high-performance liquid chromatography coupled with diode-array detection and electrospray ionization mass spectrometry (LC-ESI-MS) techniques. According to GC-MS analysis, 9-tricosene, nonadecane, 1-nonadecene and nonacosane predominated in the floral extracts. The studied samples were also characterized by relatively high amounts of benzenoids, e.g. methyl cinnamate, which is known as an attractant to the males of various orchid bees. LC-ESI-MS revealed the presence of flavor compounds such as vanillin (4-hydroxy-3-methoxybenzaldehyde) and its derivative acetovanillone, together with higher amounts of aliphatic and phenolic acids. Additionally, we detected the presence of indole and morphine derivatives.

scholarly journals Major Anthocyanin Pigments in the Ficus padana Fruits: HPLC-DAD-ESI-MS Identification and Antioxidant Activity

2014 ◽  
Vol 14 (3) ◽  
pp. 297-303 ◽  
Author(s):  
Daimon Syukri ◽  
Djaswir Darwis ◽  
Adlis Santoni
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Published Data ◽  
Ionization Mass ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Mass Spectral ◽  
Esi Ms ◽  
Array Detection ◽  
The Individual

The anthocyanins in Ficus padana were extracted and identified by using high-performance liquid chromatography/diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI-MS). The individual anthocyanins were identified by comparing between their mass spectral data and published data. The first compound (peak 1) was identify as a pelargonidin 3-(6”-p-coumarylglucoside)-5-(4”’-Malonylglucoside) and the second compound was identify as a pelargonidin 3-(6’’-Malonylglucoside). The second one is a major compound that taking up to 91% of the total anthocyanin content in F. padana extract. The antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays and results showed that extract possessed high antioxidant capacity.

scholarly journals Quantitative analysis of phenolic compounds in crude extracts of Myrcia splendens leaves by HPLC-ESI-MS/MS

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Camila Jeriane Paganelli ◽  
Diogo Alexandre Siebert ◽  
Luciano Vitali ◽  
Gustavo Amadeu Micke ◽  
Michele Debiasi Alberton
Keyword(s):  
Salicylic Acid ◽  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Ethyl Acetate ◽  
Ellagic Acid ◽  
Syringic Acid ◽  
Coumaric Acid ◽  
Southern Brazil ◽  
Esi Ms

Abstract Myrcia splendens is popularly known as “guamirim-de-folha-miúda”, and its occurrence ranges from Mexico to southern Brazil. The aim of this work was to identify and quantify phenolic compounds in the crude hydroalcoholic (EBH), ethyl acetate (EBAE) and dichloromethane (EBDM) extracts using the HPLC-ESI- MS/MS. In total, 15 compounds, including protocatecuic acid, syringic acid, p-coumaric acid, salicylic acid, isoquercetin, ellagic acid, ferulic acid, umbelliferone, coniferaldehyde, sinapaldehyde, carnosol, gallic acid, syringaldehyde, umbelliferone, coniferaldehyde, myricetin and kaempferol were identified. Ellagic acid was the major compound in all extracts.

scholarly journals Probing the floral developmental stages, bisexuality and sex reversions in castor (Ricinus communis L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sujatha Thankeswaran Parvathy ◽  
Amala Joseph Prabakaran ◽  
Thadakamalla Jayakrishna
Keyword(s):  
Developmental Stages ◽  
Ricinus Communis ◽  
Sex Expression ◽  
Pistil Abortion ◽  
Ricinus Communis L ◽  
Sex Reversion ◽  
Stage 4 ◽  
Meristem Development ◽  
Male Specific ◽  
First Time

AbstractCastor (Ricinus communis L) is an ideal model species for sex mechanism studies in monoecious angiosperms, due to wide variations in sex expression. Sex reversion to monoecy in pistillate lines, along with labile sex expression, negatively influences hybrid seed purity. The study focuses on understanding the mechanisms of unisexual flower development, sex reversions and sex variations in castor, using various genotypes with distinct sex expression pattern. Male and female flowers had 8 and 12 developmental stages respectively, were morphologically similar till stage 4, with an intermediate bisexual state and were intermediate between type 1 and type 2 flowers. Pistil abortion was earlier than stamen inhibition. Sex alterations occurred at floral and inflorescence level. While sex-reversion was unidirectional towards maleness via bisexual stage, at high day temperatures (Tmax > 38 °C), femaleness was restored with subsequent drop in temperatures. Temperature existing for 2–3 weeks during floral meristem development, influences sexuality of the flower. We report for first time that unisexuality is preceded by bisexuality in castor flowers which alters with genotype and temperature, and sex reversions as well as high sexual polymorphisms in castor are due to alterations in floral developmental pathways. Differentially expressed (male-abundant or male-specific) genes Short chain dehydrogenase reductase 2a (SDR) and WUSCHEL are possibly involved in sex determination of castor.

scholarly journals The root endophytic bacterial community of Ricinus communis L. resembles the seeds community more than the rhizosphere bacteria independent of soil water content

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stephanie E. Hereira-Pacheco ◽  
Yendi E. Navarro-Noya ◽  
Luc Dendooven
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Water Content ◽  
Soil Water ◽  
Soil Water Content ◽  
Plant Development ◽  
Bacterial Community Structure ◽  
Ricinus Communis ◽  
Ricinus Communis L ◽  
Endophytic Bacterial Community

AbstractRhizosphere and root endophytic bacteria are crucial for plant development, but the question remains if their composition is similar and how environmental conditions, such as water content, affect their resemblance. Ricinus communis L., a highly drought resistant plant, was used to study how varying soil water content affected the bacterial community in uncultivated, non-rhizosphere and rhizosphere soil, and in its roots. Additionally, the bacterial community structure was determined in the seeds of R. communis at the onset of the experiment. Plants were cultivated in soil at three different watering regimes, i.e. 50% water holding capacity (WHC) or adjusted to 50% WHC every two weeks or every month. Reducing the soil water content strongly reduced plant and root dry biomass and plant development, but had little effect on the bacterial community structure. The bacterial community structure was affected significantly by cultivation of R. communis and showed large variations over time. After 6 months, the root endophytic bacterial community resembled that in the seeds more than in the rhizosphere. It was found that water content had only a limited effect on the bacterial community structure and the different bacterial groups, but R. communis affected the bacterial community profoundly.

Sign in / Sign up

Export Citation Format

Share Document