scholarly journals Comparison of Thyroid -Stimulating Hormone and Free Thyroxine Immunoassays Performed on Immulite 2000 and Maglumi 800 Automated Analyzers

2020 ◽  
Vol 8 (B) ◽  
pp. 168-174
Author(s):  
Sonja Kuzmanovska ◽  
Daniela Miladinova
Keyword(s):  
Regression Analysis ◽  
Thyroid Stimulating Hormone ◽  
T Test ◽  
Test Method ◽  
Analytical Performance ◽  
Good Precision ◽  
Serum Samples ◽  
Significant Difference ◽  
Paired T Test ◽  
Stimulating Hormone

OBJECTIVE: Our goal was to evaluate the analytical performance of the novel immunoassay platform and to compare the agreement between thyroid-stimulating hormone (TSH) and FT4 results, obtained by novel and currently used platform. MATERIALS AND METHODS: Both immunoassay platforms, current Immulite 2000 and novel Maglumi 800, are based on chemiluminecsence immunoassay method. Analytical performance was evaluated by the use of serum pools and commercial quality control samples. The comparison study was carried out with 80 serum samples. Obtained results were analyzed by descriptive statistics, Mann–Whitney U-test, and Paired t-test. Method comparison was performed with Passing-Bablok regression analysis and Bland-Altman plots. RESULTS: TSH Maglumi 800 showed better within-run precision for both concentration ranges (1.7–2.8 CV%) in comparison to Immulite 2000 (4.4–5.7 CV%). FT4 Maglumi 800 imprecision was higher compared with Immulite 2000 FT4 in both within-run (3.5–3.9 CV% vs. 4.9–6.6 CV%) and between-run (3.6–4.2 CV% vs. 4.6–5.9 CV%) tests. Mann–Whitney U-test for TSH revealed non-significant difference between data (p = 0.9011). Regression analysis showed no systematic (intercept = 0.01), nor proportional (slope = 0.9781) differences. Non-significant bias was observed in Bland-Altman Plots. For FT4, we found significant differences between methods using paired t-test (t39 = 10.5, p < 0.0001) and significant difference (p = 0.00745) with Mann–Whitney U-test. Bland-Altman plot revealed 22.8% average bias. CONCLUSION: TSH evaluation showed good precision and close agreement between Maglumi 800 and Immulite 2000 methods, which assures transferability of results. However, FT4 performance evaluation revealed higher imprecision of Maglumi 800 platform and significant differences of test results.

scholarly journals Perbedaan Tingkat Daya Tahan Kardiorespirasi Pada Peserta Ekstrakulikuler Bolavoli dan Bola Basket

2020 ◽  
Vol 5 (2) ◽  
pp. 114-121
Author(s):  
Jeff Agung Perdana ◽  
Yudo Harvianto
Keyword(s):  
Human Activities ◽  
Female Students ◽  
T Test ◽  
Test Method ◽  
Male Students ◽  
Significance Level ◽  
Fitness Test ◽  
Significant Difference ◽  
The Difference ◽  
Paired T Test

Cardiorespiratory endurance in sports is very important. Because cardiorespiration is closely related to human activities doing work and moving. This research is motivated by cardiorespiratory fitness which is closely related to human activities doing work and moving and exercising. This study aims to determine the differences in cardiorespiration endurance among students who take extracurricular volleyball and basketball. This study uses a comparative research design with the test method. The subjects of this study were 19 basketball basketball extracurricular students and 20 volleyball extracurricular students. Data collection techniques in this study used a measurement test. The instrument used in this study was a multistage fitness test. The analysis technique used is the t-test (Paired t-Test). The results of statistical analysis showed that there was a significant difference between cardiorespiration endurance between students who took extracurricular volleyball and basketball with male students t count = 3.354 greater than t (0.05) (9) = 2.36 and female students that t arithmetic = 3.053 is greater than t (0.05) (8) = 2.31 at a significance level of 5%. The difference in cardiorespiration endurance between students who take extracurricular volleyball and basketball is male students by 13.31% and female students 18.64%.Keywords: Basketball, volleyball, cardiorespiration, Sport, Student AbstrakKetahanan kardiorespirasi dalam berolahraga sangatlah penting. Sebab kardiorespirasi erat kaitannya dengan kegiatan manusia melakukan pekerjaan dan bergerak. Penelitian ini dilatarbelakangi oleh kebugaran kardiorespirasi yang erat kaitannya dengan kegiatan manusia melakukan pekerjaan dan bergerak serta berolahraga. Penelitian ini bertujuan untuk mengetahui perbedaaan daya tahan kardiorespirasi antara  peserta didik yang mengikuti ekstrakutikuler bolavoli dan bolabasket. Penelitian ini menggunakan desain penelitian kompratif dengan metode tes. Subjek penelitian ini adalah 19 siswa ekstrakurikuler bolabasket dan 20 siswa ekstrakurikuler bola voli.  Teknik pengambilan data dalam penelitian ini menggunakan tes pengukuran. Instrumen yang digunakan dalam penelitian ini adalah multistage fitness test. Teknik analisis yang digunakan yaitu uji-t ( Paired t-Test ). Hasil analisis statistika diperoleh hasil bahwa ada perbedaan yang signifikan antara daya tahan kardiorespirasi antara  peserta didik yang mengikuti ekstrakutikuler bolavoli dan bolabasket dengan siswa putra t hitung = 3,354 lebih besar dari t(0,05)(9)  = 2,36 dan siswa putri bahwa t hitung = 3,053 lebih besar dari t(0,05)(8)  = 2,31 pada taraf signifikansi 5%. Perbedaan daya tahan kardiorespirasi antara peserta didik yang mengikuti ekstrakutikuler bolavoli dan bolabasket adalah siswa putra sebesar 13,31% dan siswa putri 18,64%.Kata kunci: Bola basket, bola voli, kardiorespirasi, olahraga, siswa

scholarly journals Emotional Freedom Techniques for Reducing Anxiety and Cortisol Level in Pregnant Adolescent Primiparous

2018 ◽  
Vol 7 (1) ◽  
pp. 1-6
Author(s):  
Mardjan Mardjan ◽  
Yayi Suryo Prabandari ◽  
Mohammad Hakimi ◽  
Carla R. Marchira
Keyword(s):  
Cortisol Level ◽  
Linear Regression Analysis ◽  
Reproductive Organs ◽  
T Test ◽  
Test Method ◽  
Third Trimester ◽  
The Third ◽  
Significant Difference ◽  
Emotional Freedom Techniques ◽  
Paired T Test

ABSTRACT Anxiety during pregnancy in  primiparous mother will be a hard burden because of the immature both psycologic and reproductive organs which can increase the risk of maternal mortality, infant mortality, prolonged childbirth, LBW, postpartum depression, etc. An effort to minimize the anxiety is the implementation of EFT (Emotional Freedom Techniques) during the third trimester.  This research purposed to assess the effectiveness of EFT to decrease anxiety in facing childbirth. This research used the quasi-experimental pre-test and post-test method of treatment and control. The treatment was done during the third trimester, started and followed for 3 months ie month 7th, 8th, 9th. The EFT was implemented every month then continued independently by the mother, until before childbirth process. The research instrument used TMAS (Taylor Manifest Anxiety Scale) and cortisol blood test. The subjects were 38 respondents consisted of 19 interventions and 19 controls. Result with paired t-test, TMAS1,2,3, each stage got significant difference, pre and post blood cortisol level p = 0.0001. Linear regression analysis on TMAS p = 0.001 and R² = 0.57, whereas blood cortisol level p = 0.004 and R² = 0.43. This analysis proved EFT contributed significantly 57% to lower anxiety levels and 43% to lower blood cortisol level, indirectly affected the readiness to face childbirth process.                                                            ABSTRAK         Kecemasan selama kehamilan pada ibu primipara akan memberatkan kondisi bayi dalam kandungan karena secara psikologis kejiwaannya belum siap dan organ reproduksi belum sempurna yang dapat meningkatkan risiko dalam persalinan dan merupakan salah satu faktor penyebab kematian ibu, bayi, partus lama, BBLR, depresi postpartum, dll. Upaya meminimalisasi kecemasan ini dilakukan dengan metode EFT (Emotional Freedom Techniques) selama trimester ketiga.         Tujuan penelitian untuk mengetahui efektivitas EFT terhadap penurunan kecemasan dalam menghadapi persalinan. Penelitian ini menggunakan metode quasi eksperimen pre test dan post terhadap perlakuan dan kontrol. Perlakuan  dilakukan selama trimester III, dimulai dan diikuti selama 3 bulan yaitu  bulan ke-7, 8, 9. EFT dilakukan setiap bulan dan dilanjutkan secara mandiri oleh ibu, sampai menjelang persalinan. Instrumen penelitian menggunakan TMAS (Taylor Manifest Anexity Scale) dan pemeriksaan darah kortisol. Subyek penelitian 38 responden, terdiri atas 19 intervensi dan 19 kontrol.         Hasil penelitian  dengan uji paired t-test, TMAS1,2,3, setiap tahapannya didapatkan perbedaan bermakna yaitu kortisol darah pre dan post p=0,0001, analisa regresi liniear TMAS   p = 0,001, dan R² = 0,57; serta kortisol darah p=0,004 dan R²=0,43. Analisa ini membuktikan EFT berkontribusi 57% menurunkan tingkat kecemasan dan 43%  dalam menurunkan kortisol darah secara signifikan yang secara tidak langsung  berpengaruh terhadap kesiapan menghadapi persalinan.   

The effect of positive thinking training on the quality of life of parents of adolescent with thalassemia

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Adeleh Sadeghloo ◽  
Parna Shamsaee ◽  
Elham Hesari ◽  
Golbanhar Akhondzadeh ◽  
Hamid Hojjati
Keyword(s):  
Quality Of Life ◽  
Emotional Health ◽  
Intervention Group ◽  
T Test ◽  
World Health ◽  
Positive Thinking ◽  
Significant Difference ◽  
And Control ◽  
Paired T Test

Abstract Introduction Thalassemia, as the most common chronic hereditary blood disorder in humans, can impose many adverse effects on parents and their affected children. On the other hand, positive thinking is the method or the result of a positive focus on a constructive issue, so by teaching positive thinking, we can improve physical and social function, and promote emotional health and overall quality of life. The aim of this study was to determine the effect of positive thinking training on the quality of life of parents of adolescents with thalassemia. Methods This quasi-experimental study was performed on 52 parents of adolescents with thalassemia who attended Taleghani Hospital in Golestan province, Iran in 2017. Samples were randomly assigned into two groups of intervention and control. The positive thinking training (based on the theory of Martin Seligman) was carried out in the intervention group in 10 (45–60 min) sessions over a 5-week period. Data collection tools in this study were the World Health Organization (WHO) quality of life questionnaire (WHOQOL-BREF) and a demographic information questionnaire. Data were analyzed by SPSS-16 software using independent t-test, the paired t-test and a covariance test. Results The results of paired t-test showed a significant difference in the score of quality of life in intervention group before and after the intervention (p < 0.001). However, this test did not show any significant difference in the control group (p = 0.11). The covariance test, after removing the pre-test score, showed a significant difference between the intervention and control groups in terms of the score of quality of life, so that 13% of the changes after the intervention were due to the intervention (p = 0.009, η = 0.13). Conclusion The positive thinking training increased the quality of life of parents of adolescents with thalassemia. Therefore, using this program can be an effective way of improving the quality of life of parents. Thus, positive thinking training is suggested to be used as an effective strategy for increasing the quality of life of parents with ill children.

Percentile transformation and recalibration functions allow harmonization of thyroid-stimulating hormone (TSH) immunoassay results

2020 ◽  
Vol 58 (10) ◽  
pp. 1663-1672 ◽  
Author(s):  
Andrea Padoan ◽  
Aldo Clerico ◽  
Martina Zaninotto ◽  
Tommaso Trenti ◽  
Renato Tozzoli ◽  
...  
Keyword(s):  
Robust Regression ◽  
Reference Intervals ◽  
Thyroid Stimulating Hormone ◽  
Validation Dataset ◽  
New Approach ◽  
Coefficients Of Variation ◽  
Significant Difference ◽  
Before And After ◽  
Roche Diagnostics ◽  
Stimulating Hormone

AbstractBackgroundThe comparability of thyroid-stimulating hormone (TSH) results cannot be easily obtained using SI-traceable reference measurement procedures (RPMs) or reference materials, whilst harmonization is more feasible. The aim of this study was to identify and validate a new approach for the harmonization of TSH results.MethodsPercentile normalization was applied to 125,419 TSH results, obtained from seven laboratories using three immunoassays (Access 3rd IS Thyrotropin, Beckman Coulter Diagnostics; Architect System, Abbott Diagnostics and Elecsys, Roche Diagnostics). Recalibration equations (RCAL) were derived by robust regressions using bootstrapped distribution. Two datasets, the first of 119 EQAs, the second of 610, 638 and 639 results from Access, Architect and Elecsys TSH results, respectively, were used to validate RCAL. A dataset of 142,821 TSH values was used to derive reference intervals (RIs) after applying RCAL.ResultsAccess, Abbott and Elecsys TSH distributions were significantly different (p < 0.001). RCAL intercepts and slopes were −0.003 and 0.984 for Access, 0.032 and 1.041 for Architect, −0.031 and 1.003 for Elecsys, respectively. Validation using EQAs showed that before and after RCAL, the coefficients of variation (CVs) or among-assay results decreased from 10.72% to 8.16%. The second validation dataset was used to test RCALs. The median of between-assay differences ranged from −0.0053 to 0.1955 mIU/L of TSH. Elecsys recalibrated to Access (and vice-versa) showed non-significant difference. TSH RI after RCAL resulted in 0.37–5.11 mIU/L overall, 0.49–4.96 mIU/L for females and 0.40–4.92 mIU/L for males. A significant difference across age classes was identified.ConclusionsPercentile normalization and robust regression are valuable tools for deriving RCALs and harmonizing TSH values.

scholarly journals EFFECTIVENESS OF GLUTEAL MUSCLE STRENGTHENING ON FLAT FOOT

2017 ◽  
Vol 10 (6) ◽  
pp. 219
Author(s):  
Pooja A Mulchandani ◽  
Trupti Warude ◽  
Amrutkuvar Pawar
Keyword(s):  
T Test ◽  
P Value ◽  
Random Allocation ◽  
Gluteal Muscle ◽  
Flat Foot ◽  
Muscle Strengthening ◽  
Intrinsic Muscle ◽  
Navicular Drop ◽  
Significant Difference ◽  
Paired T Test

Objectives: To compare the effect of gluteal muscle strengthening along with conventional exercises versus conventional exercises alone on flat foot.Method: An experimental study conducted at Physiotherapy Department of Krishna Institute of Medical Sciences, Karad. A total of 52 subjects were equally divided into two groups using convenient sampling with random allocation (Groups A and B). Baseline treatment was given to both groups (intrinsic muscle strengthening). Group A was given intrinsic muscle strengthening alone while Group B was given gluteal muscle strengthening along with intrinsic muscle strengthening.Result: Statistical analysis was performed using paired t-test and unpaired t-test. In pre-intervention there was no statistically significant difference seen with p values for the navicular drop was 0.3563 and for Ink test was 0.7342. While on comparing the post-interventional values, the results between the two groups using paired t-test revealed that there was extremely significant difference seen with p-value for the navicular drop was <0.0001 and for Ink test was <0.0001.Conclusion: From the study, it can be concluded that there was a significant effect of gluteal muscle strengthening on the flat foot.

Agreement analysis comparing iPad LCVA and Sloan testing in multiple sclerosis patients

2017 ◽  
Vol 24 (8) ◽  
pp. 1126-1130 ◽  
Author(s):  
Neda Sattarnezhad ◽  
Samantha Farrow ◽  
Dorlan Kimbrough ◽  
Bonnie Glanz ◽  
Brian Healy ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Visual Acuity ◽  
T Test ◽  
Computerized Testing ◽  
Low Contrast ◽  
Significant Difference ◽  
Increased Sensitivity ◽  
The Right ◽  
Multiple Sclerosis Patients ◽  
Paired T Test

Background: Visual symptoms are common in multiple sclerosis (MS). Low-contrast visual acuity (LCVA) testing using Sloan charts has demonstrated increased sensitivity for visual deficits compared to high-contrast acuity testing. Computerized testing of visual acuity may facilitate use in the clinic setting. Objectives: To evaluate the agreement between an iPad-based and Sloan testing of LCVA in a cohort of MS patients. Methods: A total of 38 patients with relapsing-remitting MS were enrolled after providing informed written consent at Partners MS Center, Brigham and Women’s hospital. Monocular LCVA was measured using retroilluminated Sloan chart and iPad-based LogMAR chart. Number of correct letters and agreement between two measurements were assessed for each eye using Bland–Altman analysis and paired t-test. Results: For both eyes, there was no significant difference in number correct between the two measurements using a paired t-test, and there was high correlation between two measurements (oculus dextrus (OD) r = 0.89, p < 0.001; oculus sinister (OS) r = 0.78, p < 0.001). The limits of agreement were −7.9 to +8.5 letters for the right eye and −10.9 to +11.2 letters for the left eye. Conclusion: An iPad-based LCVA test shows good agreement with Sloan testing in MS patients.

scholarly journals PERFORMANCE COMPARISON OF LASTON AC-WC BASED ON AGGREGATE GRADATION SPECIFICATIONS

2018 ◽  
Vol 2 (02) ◽  
pp. 151
Author(s):  
Devi Yulita Metasari ◽  
Akhmad Hasanuddin ◽  
Dewi Junita Koesoemawati
Keyword(s):  
Flow Rate ◽  
Outer Layer ◽  
Performance Comparison ◽  
High Flow ◽  
T Test ◽  
Aggregate Gradation ◽  
Significant Difference ◽  
Bitumen Content ◽  
Asphalt Content ◽  
Paired T Test

Laston AC-WC is the outer layer of the pavement that is directly related to the outside, so a strong mixture is required. One of the major effects is the aggregate gradation.This experiment aims to find out the comparison of Laston AC-WC performance between mixtures used aggregat egradation above and below the restriction zone. Each mixture used 6 variations of asphalt content and there were 3 specimens on each variation of bitumen content. The asphalt contents used were 5,5%; 6%; 6,5%; 7%; 7,5% dan 8%. From the results of the experiments, the mixture with the aggregate above the restriction zone at 7% and 7.5% asphalt content had a density value of 2.13 gr / cc and 2.12 gr / cc respectively; VMA value of 15.49% and 15.91%; VFA values ​​of 80.48% and 79.98%; VIM value of 3.16% and 3.18%; stability value of 3013.58 kg and 2332.57 kg; flow value of 2.47 mm and 2.18 mm; as well as MQ values ​​of 1222.25 kg / mm and 1184.59 kg / mm. While on the mixture with the aggregate under the restriction zone in the asphalt content of 7% and 7.5% obtained density value of 2.11 gr / cc and 2.14 gr / cc; VMA value of 17.21% and 16.3%; VFA value of 71.21% and 78.65%; VIM value of 4.99% and 3.49%; stability value of 2518,75 kg and 3234,72 kg; flow values ​​of 2 mm and 2.07 mm; as well as the MQ value of 1259.37 kg / mm and 1623.54 kg / mm. From the results are analyzed Paired T test and found that the value of flow has a significant difference. The high flow rate was found in the mixture with the aggregate above the restriction zone at 7% asphalt content, so this mixture is more durable due to good flexibility. Laston AC-WC merupakan lapisan terluar perkerasan yang berhubungan langsung dengan keadaan luar, sehingga dibutuhkan campuran yang kuat. Salah satu yang berpengaruh besar adalah gradasi agregat yang digunakan. Penelitian ini bertujuan untuk mengetahui perbandingan kinerja laston AC-WC antara campuran yang menggunakan gradasi di atas dengan di bawah daerah larangan. Penelitian ini dilakukan terhadap campuran laston AC-WC. Masing-masing campuran menggunakan 6 variasi kadar aspal dan terdapat 3 benda uji pada setiap kadar aspal. Kadar aspal yang digunakan adalah 5,5%; 6%; 6,5%; 7%; 7,5% dan 8%. Dari hasil penelitian, campuran dengan agregat di atas daerah larangan pada kadar aspal 7% dan 7,5% memiliki nilai density berturut-turut sebesar 2,13 gr/cc dan 2,12 gr/cc; nilai VMA sebesar 15,49% dan 15,91%; nilai VFA sebesar 80,48% dan 79,98%; nilai VIM sebesar 3,16% dan 3,18%; nilai stabilitas sebesar 3013,58 kg dan 2332,57 kg; nilai flow sebesar 2,47 mm dan 2,18 mm; serta nilai MQ sebesar 1222,25 kg/mm dan 1184,59 kg/mm. Sedangkan pada campuran dengan agregat di bawah daerah larangan pada kadar aspal 7% dan 7,5% didapatkan nilai density sebesar 2,11 gr/cc dan 2,14 gr/cc; nilai VMA sebesar 17,21% dan 16,3%; nilai VFA sebesar 71,21% dan 78,65%; nilai VIM sebesar 4,99% dan 3,49%; nilai stabilitas sebesar 2518,75 kg dan 3234,72 kg; nilai flow sebesar 2 mm dan 2,07 mm; serta nilai MQ sebesar 1259,37 kg/mm dan 1623,54 kg/mm. Dari hasil tersebut dilakukan analisa Uji T Berpasangan dan didapatkan bahwa nilai flow memiliki perbedaan yang signifikan. Nilai flow yang tinggi terdapat pada campuran dengan agregat di atas daerah larangan pada kadar aspal 7%, sehingga campuran ini lebih tahan lama dikarenakan memiliki kelenturan yang baik.

scholarly journals Comparison of Copper Level and Color Vision in Pulmonary Tuberculosis Patients Before and After Ethambutol Treatment in Category I and II

2020 ◽  
Vol 3 (1) ◽  
pp. 19-25
Author(s):  
Febria Restissa ◽  
Ahmad Rasyid ◽  
Devi Azri Wahyuni
Keyword(s):  
Color Vision ◽  
Total Error ◽  
T Test ◽  
Wilcoxon Test ◽  
Copper Level ◽  
Tuberculosis Patients ◽  
Vision Disorder ◽  
Significant Difference ◽  
Before And After ◽  
Paired T Test

ABSTRACT Objective : to determine the correlation between duration of ethambutol and copper levels in serum with color vision disorder examined with Farnsworth Munsell 100 Hue (FM100) in patients with tuberculosis. Methods: This study was an explorative observation of 20 samples during November 2017 - January 2018 period. Comparative analysis using independent t-test, paired t-test, Wilcoxon test and Mann-Whitney test, while correlation analysis using Spearman's test and Pearson's test.  Results: There was a significant difference of FM100 value before and after in category I (p=0.000) with increase of total error value 11.80+5.31 or category II (p=0.000) with increase of total error value 15.00+7.41. There was significant difference of Cu2+ level before and after in category I (p=0.005) with decrease of Cu2+ level 15.57+5.04 mg/dL or category II (p=0.005) with decrease of Cu2+ level 31.66+7.35 mg/dL. There was significant correlation between color vision and duration of ethambutol (p=0.000), but there was no significant correlation of Cu2+ with color vision (p>0.05). Conclusion: The duration of Ethambutol was related to changes in color vision examined with FM100.

scholarly journals Analysis The Level Of Cattleman’s Knowledge Using The T-Test And Wilcoxon In Maju Farmer Group

2020 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Bismi Widi Hastari ◽  
Dyah Gandasari ◽  
Harry Harry
Keyword(s):  
T Test ◽  
Statistical Software ◽  
City Administration ◽  
Group Data ◽  
Significant Difference ◽  
Urine Production ◽  
Before And After ◽  
Post Test ◽  
Level Of Knowledge ◽  
Paired T Test

Tani Maju farmer group is one of the farming groups located in Rorotan Village, Cilincing District, North Jakarta City Administration. This group has a lot of sheep and advanced management. Despite they manage their sheep well, they are not well informed on how to manage the sewage from their castles (faces and urine). So far, they just throw away the sewage or gave them to fellow farmers. This study aimed to analyze the level of farmers' knowledge in making use of sheep's urine to be degraded into bio urine in the Maju Farmer group. Data was collected through the pre-test and post-test. The data were analyzed using paired t-test and Wilcoxon statistical software (IBM SPSS Statistics 25). The results of the study showed that farmers' knowledge in sewage (Urine) management increased; there is a significant difference in farmers' level of knowledge in bio urine production before and after bio urine extension.

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