Bcl‑xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF‑β/BMP expression levels

Abstract Background: Adipose-derived stem cells ( ADSCs ) therapy is considered as a promising alternative to treat osteoarthritis (OA).Considerable evidence has shown that the Wnt signaling pathway is involved in chondrogenic differentiation in various stem cells.In the present study, we explored in detail the mechanism of of Wnt signaling pathway in the regulation of adipose-derived stem cell chondrogenic differentiation.Methohs: Adipose-derived stem cells were extracted from Sprague-Dawley groin area, isolated, cultured and verified. Then LiCl and DKK-1 used to induce ADSCs cartilage differentiation to detect indicators of cartilage at different stages, such as collagen type II (Collagen2a), aggrecan (Aggrecan) and Wnt pathway key proteins Sox9, β-catenin, Glycogen synthase kinase-3β (GSK-3β) expression.Subsequently, lentivirus was used as the vector, the full-length Sox9 gene was transfected into ADSCs and the expression levels of relevant indicators were detected again after stable expression.Results: In the process of inducing differentiation, the Wnt pathway promoted the rapid proliferation of ADSCs in the early, middle and late stages, and up-regulates Sox9 expression.Cartilage indicators Collagen 2a and Aggrecan expression levels were not significantly increased in the early stage, but significantly increased in the middle and late stages (P <0.05). At the later stage, however, the Wnt pathway down-regulated Sox9 expression, weakened the mature cartilage phenotype, promoted cartilage hypertrophy and early cartilage ossification. Finally, the overexpression of Sox9 feedback inhibits the Wnt pathway activity, down-regulates β-catenin expression, maintains cartilage phenotype, delays cartilage hypertrophy and early osteogenesis.Conclusion:The Wnt pathway regulates chondrogenic differentiation of ADSCs by regulating Sox9, but its regulatory mechanism is significantly different at different stages of induced differentiation.

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Investigation of SPARC protein expression levels at dopaminergic system in mouse brain treated with repeated morphine administration

Neuroscience Research ◽

10.1016/s0168-0102(00)81595-3 ◽

2000 ◽

Vol 38 ◽

pp. S125

Author(s):

S Akiduki

Keyword(s):

Protein Expression ◽

Mouse Brain ◽

Dopaminergic System ◽

Expression Levels ◽

Morphine Administration

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Gene expression analysis of metabolic markers during bone regeneration in a rat model

Osteologie/Osteology ◽

10.1055/s-0037-1620051 ◽

2014 ◽

Vol 23 (03) ◽

pp. 207-211

Author(s):

C. Kasch ◽

A. Osterberg ◽

Thordis Granitzka ◽

T. Lindner ◽

M. Haenle ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Analysis ◽

Saline Solution ◽

Female Rats ◽

Synthesis Rate ◽

Metabolic Markers ◽

Expression Levels ◽

Fibrotic Tissue ◽

Intermittent Pth ◽

Lower Expression

SummaryThe RANK/RANKL/OPG system plays an important role in the regulation of bone metabolism and bony integration around implants. The aim of this study was to analyse gene expression of OPG, RANK, and RANKL in regenerating bone during implant integration. Additionally, the effect of intermittent para - thyroid hormone (PTH) treatment was analysed. A titanium chamber was implanted in the proximal tibiae of 48 female rats. The animals received either human PTH or saline solution (NaCl). After 21 and 42 days, RNA was isolated from tissue adjacent to the implant and expression of RANK, RANKL, and OPG was analysed. After 21 days, very low expression levels of all genes were shown. In contrast, increased gene expression after 42 days was determined. Expression of RANK and RANKL was lower than that for OPG. The lower expression levels after 21 days might be due to still ossifying, fibrotic tissue around the titanium chamber. An increased OPG synthesis rate associated with decreased RANKL expression after 42 days revealed bone-forming processes. Despite significant differences in gene expression between the time points, only slight differences were observed between application of intermittent PTH and NaCl after a period of 42 days.

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High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656335 ◽

1992 ◽

Vol 68 (02) ◽

pp. 119-124 ◽

Cited By ~ 10

Author(s):

F G Falkner ◽

P L Turecek ◽

R T A MacGillivray ◽

W Bodemer ◽

F Scheiflinger ◽

...

Keyword(s):

Vaccinia Virus ◽

Cell Lines ◽

Expression System ◽

Human Cell Line ◽

Cell System ◽

Time Saving ◽

Expression Levels ◽

Mammalian Cell Lines ◽

Cell Line Hela ◽

Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

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Gene expression levels of Casein kinase 1 (CK1) isoforms are correlated to adiponectin levels in adipose tissue and site-specific phosphorylation mediated by CK1 influences multimerization of adiponectin

Zeitschrift für Gastroenterologie ◽

10.1055/s-0038-1669041 ◽

2018 ◽

Vol 56 (08) ◽

pp. e345-e346

Author(s):

P Xu ◽

P Fischer-Posovszky ◽

M Wabitsch ◽

U Knippschild

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Casein Kinase ◽

Casein Kinase 1 ◽

Site Specific ◽

Expression Levels ◽

Gene Expression Levels

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Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽

10.1530/rep-18-0143 ◽

2018 ◽

Cited By ~ 1

Author(s):

Pacharawan Deenarn ◽

Punsa Tobwor ◽

Rungnapa Leelatanawit ◽

Somjai Wongtriphop ◽

Jutatip Khudet ◽

...

Keyword(s):

Fatty Acid ◽

Ovarian Development ◽

Penaeus Monodon ◽

Chain Fatty Acid ◽

Regulatory Genes ◽

Ovarian Maturation ◽

Long Chain Fatty Acid ◽

Eyestalk Ablation ◽

Regulatory Pathways ◽

Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

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