Ethanol extracts of chickpeas alter the total lipid content and expression levels of genes related to fatty acid metabolism in mouse 3T3-L1 adipocytes

Fish production and fish processing waste have straight connection. In India, the waste produced during the processing of fish is predicted to be approximate 3.6 million metric tonnes, 48 per cent of the total body weight of Indian and exotic major carps is thrown away as waste (non-edible ). The present research, it was conducted to compare the total lipid content (TLC) and fatty acid composition from the liver of captured and cultured fish, Labeo rohita (Hamilton) having weight more than 500 gram during different months as well as to evaluate its nutritional quality. Maximum total lipid content (33.33±0.14%) was found in the liver of cultured fish in May month, while the minimum (15.26±0.24%) was in the liver of captured fish in the month of January. During the study, total lipid content was found to be considerably elevated in cultured than captured Fish, Labeo rohita (Hamilton). The amount of three major groups of fatty acids namely polyunsaturated fatty acids, monounsaturated fatty acids and saturated fatty acids was found to be maximum in captured fish during the month of March 97.19±0.96%, 61.30±0.56% and 95.39±0.31% month of April respectively. Total n-6 polyunsaturated fatty acids were observed to be highest (21.95±8.05%) in the waste of liver in cultured fish during the month of March. Hence, it is concluded that the processing waste (liver) of the captured and cultured, Labeo rohita (Hamilton) is a prosperous resource of the essential fatty acids i.e. PUFAs and total lipids. Further, it is observed that captured species are rich in fatty acid composition as compared to cultured species. Food industries can manufacture by-products from these high nutritional value contents of waste for human utilization. EPA and DHA also reduce the risk of various life threatening diseases.

Download Full-text

Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain

Journal of Chemistry ◽

10.1155/2020/8651453 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Thi Phuong Lan Nguyen ◽

Van Tuyen Anh Nguyen ◽

Thi Thanh Trung Do ◽

Trung Nguyen Quang ◽

Quoc Long Pham ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Total Lipid ◽

Lipid Content ◽

High Resolution Mass Spectrometry ◽

Total Lipid Content ◽

Molecular Forms ◽

Scylla Paramamosain ◽

Mud Crab ◽

Anti Inflammatory

The mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. The total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. The composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. The anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time. The anti-inflammatory activity of the total and polar lipids had IC50 values of 71.5 and 68.6 μg/mL, respectively. The crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC50 values ranging from 85.4 to 95.8 μg/mL.

Download Full-text

Fatty acid composition and total lipid content in the lancelet Branchiostoma belcheri tsingtaoense (Tchang et Koo)

Comparative Biochemistry and Physiology Part A Physiology ◽

10.1016/0300-9629(94)90102-3 ◽

1994 ◽

Vol 108 (2-3) ◽

pp. 325-329 ◽

Cited By ~ 4

Author(s):

V.I Svetashev ◽

Hefang Li ◽

Yu Fu Cai ◽

Zuo-qing Sun ◽

Nian-hong Chen ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Total Lipid ◽

Lipid Content ◽

Total Lipid Content ◽

Branchiostoma Belcheri

Download Full-text

Anti-IL-17 Antibody Improves Hepatic Steatosis by Suppressing Interleukin-17-Related Fatty Acid Synthesis and Metabolism

Clinical and Developmental Immunology ◽

10.1155/2013/253046 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

Weidong Shi ◽

Qiang Zhu ◽

Jian Gu ◽

Xiaoshan Liu ◽

Ling Lu ◽

...

Keyword(s):

Fatty Acid ◽

Liver Disease ◽

Hepatic Steatosis ◽

Fatty Acid Metabolism ◽

Alcoholic Liver Disease ◽

Acid Metabolism ◽

Binding Protein ◽

Interleukin 17 ◽

Expression Levels ◽

Element Binding Protein

To investigate the relationship between interleukin-17 and proteins involved in fatty acid metabolism with respect to alcoholic liver disease, male ICR mice were randomized into five groups: control, alcoholic liver disease (ALD) at 4 weeks, 8 weeks, and 12 weeks, and anti-IL-17 antibody treated ALD. A proteomic approach was adopted to investigate changes in liver proteins between control and ALD groups. The proteomic analysis was performed by two-dimensional difference gel electrophoresis. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. Additionally, expression levels of selected proteins were confirmed by western blot. Transcriptional levels of some selected proteins were determined by RT-PCR. Expression levels of 95 protein spots changed significantly (ratio >1.5,P<0.05) during the development of ALD. Sterol regulatory element-binding protein-lc (SREBP-1c), carbohydrate response element binding protein (ChREBP), enoyl-coenzyme A hydratase (ECHS1), and peroxisome proliferator-activated receptor alpha (PPAR-α) were identified by MS/MS among the proteins shown to vary the most; increased IL-17 elevated the transcription of SREBP-1c and ChREBP but suppressed ECHS1 and PPAR-α. The interleukin-17 signaling pathway is involved in ALD development; anti-IL-17 antibody improved hepatic steatosis by suppressing interleukin-17-related fatty acid metabolism.

Download Full-text