Antidiabetic Activity of Aqueous Leaf Extracts of Guazuma ulmifolia Lamk., Ethanolic Extracts of Curcuma xanthorrhiza and their Combinations in Alloxan-induced Diabetic Mice

2013 ◽  
Vol 7 (3) ◽  
pp. 158-164 ◽  
Author(s):  
I.K. Adnyana ◽  
E. Yulinah ◽  
Yuliet . ◽  
N.F. Kurniati
Keyword(s):  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Leaf Extracts ◽  
Ethanolic Extracts ◽  
Guazuma Ulmifolia ◽  
Curcuma Xanthorrhiza

scholarly journals Antidiabetic activity of tannin fraction of Bridelia ferruginea (Benth) leaf extract on fructose-induced diabetic mice

2020 ◽  
Vol 10 (1) ◽  
pp. 68-74
Author(s):  
Sabrina Sanvee ◽  
Oudjaniyobi Simalou ◽  
Gneiny Whad Tchani ◽  
Hèzouwè Kagnou ◽  
Batomayena Bakoma ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Reducing Power ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Diabetic Mice ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Bridelia Ferruginea

Introduction: Bridelia ferruginea is a plant known for its antidiabetic properties. However, few studies on leaf extracts have induced anti-hyperglycemic activity on normal mice subjected to carbohydrate overload. The current study was designed to assess the effect of the leaf extracts’ fraction on fructose-induced diabetic mice. Methods: The in vitro ferric-reducing antioxidant power (FRAP) assay were carried out and the condensed tannins quantified. The vanillin-HCl method was used to characterize the condensed tannins. The antidiabetic effect on fructose-induced diabetic mice was evaluated for 28 days using a fructose-enriched fat diet approach. Results: The fraction confirmed the antioxidant activity with a reducing power of 800 μg/mL comparable to ascorbic acid at 200 μg/mL. The condensed tannins were estimated at 79.6 ± 3.4 mg catechin equivalent per gram of sample. Significant decreases in blood sugar levels of 6.25% at the 7th day, 11.04% at the 14th day, 12.61% at the 21th day, and 11.35% at the 28th day were obtained in mice treated with the extract dose of 200 mg/kg of body weight, compared to the positive control group. The decreases of 37.11% of triglycerides and 40.16% of total cholesterol were also obtained. Conclusion: The investigated fraction showed notable antidiabetic activity and might be a good candidate in the treatment of diabetes.

scholarly journals Exploration of Antidiabetic Activity of Stephania japonica Leaf Extract in Alloxan-Induced Swiss Albino Diabetic Mice

2019 ◽  
pp. 1-12
Author(s):  
Md. Dobirul Islam ◽  
Syeda Farida Akter ◽  
Md. Amirul Islam ◽  
Md. Salim Uddin
Keyword(s):  
Gallic Acid ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Mice Model ◽  
Leaf Extracts ◽  
Alternative Source ◽  
Dry Extract

Aims: Presently the medicinal world is rapidly turning more on the therapeutic health benefits of natural product and medicinal plants in the management of major crucial disease and their complications. Medicinal plant, Stephania japonica has been studied for exploring antidiabetic potentiality as an alternative source of medicine against the global threat of Diabetes mellitus (DM). Methods: The extraction of S. japonica leaf was carried out by acetone and ethanol. Phytochemical screening and quantitative analysis of S. japonica leaf extracts were evaluated through chemically forming characterized color formation and calibration method respectively, by using standard reference substances (ascorbic acid, gallic acid, and quercetin) to assess the probable compounds present in the extract. Anti-diabetic potentiality of highest phytochemicals containing two extracts were investigated in in vitro as a ⍺-amylase inhibitors and in vivo through alloxan-induced Swiss albino diabetes mice model. Results: Alkaloids, carbohydrates, steroids, flavonoids, resins, saponins, tannins and coumarins were present in the leaf extracts. The estimated amount of total phenolics, flavonoids, flavonols and proanthrocyanidins contents of acetone and ethanol extract were 92.12±0.64 and 56.54±1.05 mg of gallic acid equivalent (GAE)/gm of dry extract, 66.02±1.42 and 46.17±0.54 mg of catechin equivalent (CAE)/gm of dry extract, 7.05±0.108 and 5.26±0.083 mg of quercetin equivalent (QUE)/gm of dry extract, 35.19±0.67 and 9.55±1.11 mg CAE/gm of dry extract, respectively. In 3, 5-dinitrosalicylic acid method, acetone and ethanol extract showed α-amylase inhibition of 51.02% and 46.62%, respectively at the concentration of 1000 µg/mL whereas in starch iodine color assay, acetone and ethanol extract showed inhibition of 57.32% and 52.12%, respectively at the concentration of 800 µg/mL. In contrast, both of the leaf extracts significantly (p<0.05 to p<0.001) improved the lipid profile parameters, blood glucose level and serum hepatic marker proteins in alloxan-induced diabetic mice. Conclusion: The present study strongly concluded that S. japonica leaf extracts process potent antidiabetic potentiality that might be significance for the management of diabetes and its complications.

Ameliorative potential of Crinum asiaticum Linn leaf extracts on Elevated blood glucose in Alloxan-induced Diabetic Rats

2021 ◽  
pp. 6359-6363
Author(s):  
Abhinav Mishra ◽  
Vikram Singh ◽  
Raj K Prasad ◽  
Mohd Habeeb Ahmad
Keyword(s):  
Blood Glucose ◽  
Dose Level ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Oral Toxicity ◽  
Leaf Extracts ◽  
Glucose Lowering ◽  
Ethanolic Extracts ◽  
Crinum Asiaticum

In the present investigation the glucose lowering potential of the leaf extracts of Crinum asiaticum were prepared using cold maceration technique in solvents of varying polarity. The extracts exhibited the presence of flavonoids, alkaloids, phenolics and tannins. The oral toxicity of the aqueous and ethanolic extracts was determined and these two extracts were used of evaluating the antidiabetic activity. Oral glucose tolerance test was performed and diabetes was induced using alloxan (150 mg/kg) in rats. Ethanolic and aqueous extracts at two dose levels (200 mg/kg and 400mg/kg) were used for evaluating glucose lowering capability. Both the aqueous and the ethanolic extracts were found to significantly reduce glucose levels with the aqueous extract at dose level 200 mg/kg being the most effective (50% reduction) whereas the ethanolic extract was able to reduce the blood glucose by around 35% at the same dose level.

scholarly journals Phytochemical Screening and Antidiabetic, Antihyperlipidemic, and Antioxidant Effects of Leptopus Cordifolius Decne. In Diabetic Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahid Rahman ◽  
Gul Jan ◽  
Farzana Gul Jan ◽  
Hafeez Ur Rahim
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Group Treatment ◽  
Methanolic Extract ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Haemolytic Assay

Plants are well known in traditional herbal medicines for their hypoglycemic and hypolipidemic activities and are often used due to their accessibility, affordability, and corollary effects. Leptopus cordifolius has been reported to control diabetes in folkloric medicine, but no known scientific research has been conducted to assess the plausibility of this assertion. Therefore, the current study is aimed to investigate the antidiabetic and hypolipidemic effects of Leptopus cordifolius leaves in alloxan-induced diabetic mice. The antidiabetic and antihyperlipidemic evaluation was conducted in Swiss albino mice at doses of 150–250°mg/kg for 15°days. The blood glucose, total cholesterol, triglyceride, LDL, HDL, creatinine, ALP, SGPT, and SGOT levels were estimated according to standard procedures. Phytochemicals of leaves were analyzed using GC-MS analysis. Enzymatic antioxidant activity of the plant was investigated spectrophotometrically by carrying out superoxide dismutase, peroxidase, and catalase assays. The membrane stabilization potential of L. cordifolius leaf extracts was carried out using an in vitro haemolytic assay. The results revealed a dose response effect with the methanolic extract of L. cordifolius which had significant antihyperglycemic effects at 150–250°mg/kg in alloxan treated mice, although less than the positive control (glibenclamide). Hyperlipidemic activity was significant at 250 mg/kg. The biochemical parameters, such as total cholesterol, triglyceride, LDL, HDL, creatinine, ALP, SGPT, and SGOT, were significantly improved (p &lt; 0.01) by the methanolic extract of 250 mg/kg compared to the diabetic group. Treatment for 15 days showed significant elevation (p &lt; 0.01) of antioxidant enzymes. GC-MS analysis provided tentative identifications of 52 compounds in the methanolic extract of L. cordifolius, of which 12 compounds have reported antidiabetic activity. In conclusion, methanolic extract of L. cordifolius of 150 and 250°mg/kg body weight showed significant antidiabetic and antihyperlipidemic activities in alloxan-induced diabetic mice and, with further work, has the potential to be used to manage blood glucose and cholesterol levels.

scholarly journals In vivo antidiarrheal study of ethanolic extracts of Mikania cordata and Litsea monopetala leaves

2015 ◽  
Vol 10 (3) ◽  
pp. 562 ◽  
Author(s):  
Fatema Nasrin ◽  
Md. Lukman Hakim
Keyword(s):  
Body Weight ◽  
Castor Oil ◽  
Leaf Extract ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Leaf Extracts ◽  
Ethanolic Extracts ◽  
Antidiarrheal Activity

<p class="Abstract">In this study the antidiarrheal activity of ethanolic extracts of the leaves of <em>Mikania cordata</em> and <em>Litsea monopetala</em> was evaluated. Diarrhea was induced in mice by oral administration of castor oil (0.5 mL) 30 min after the administration of the extracts. During a 4 hour study the number of diarrheal feces and percentage inhibition of the extracts (200 and 400 mg/kg body weight) was determined. Loperamide (3 mg/kg body weight) served as standard and belonged to the positive control group. The extracts exhibited potent antidiarrheal activity as well as achieved statistically significant p value (p&lt;0.01 and p&lt;0.05) compared to control group. Among the extracts the highest percentage inhibition of defecation (60%) was recorded for leaf extract (400 mg/kg body weight) of <em>L. monopetala</em>. So, the study corroborates the significant antidiarrheal activity of <em>M. cordata</em> and <em>L. monopetala</em> leaf extracts and raises the demand of further sophisticated investigation.</p><p> </p>

Antidiabetic activity of polysaccharides from Suillellus luridus in streptozotocin-induced diabetic mice

2018 ◽  
Vol 119 ◽  
pp. 134-140 ◽  
Author(s):  
Lan Zhang ◽  
Yixi Liu ◽  
Yu Ke ◽  
Yuntao Liu ◽  
Xiaohu Luo ◽  
...  
Keyword(s):  
Antidiabetic Activity ◽  
Diabetic Mice

scholarly journals Phytochemical evaluation and in vitro antidiabetic efficiency of isopropanolic leaf extract of pimenta racemosa

2020 ◽  
Vol 7 (2) ◽  
pp. 50-55
Author(s):  
Anitha T A ◽  
Pakutharivu T ◽  
Nirubama K ◽  
Akshaya V
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Uptake ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Yeast Cells ◽  
Antidiabetic Activity ◽  
Leaf Extracts ◽  
Pimenta Racemosa

The traditional herbal medicines are mainly obtained from plants are used in the management of Diabetes mellitus. The main objective of this work was to assess the presence of phytochemical compounds and to evaluate the in vitro antidiabetic activity of isopropanolic extracts of Pimenta racemosa leaves by studying their α-amylase inhibitory activity and glucose transport across yeast cells. Screening of phytochemicals showed positive results for alkaloids, steroids, cardiac glycosides, terpenoids, reducing sugars, anthraquinones, and results of in vitro α-amylase inhibitory studies demonstrated there was a dose-dependent increase in percentage inhibitory activity by the isopropanolic leaf extracts of Pimenta racemosa. At a concentration of 1 mg/ml, the extract showed a percentage inhibition 33.6 and for 5 mg/ml it was 91.2. The glucose uptake study was also studied through yeast cells by analyzing theamount of glucose remaining in the medium after a specific time intervals. It serves as an indicator for the capability of isopropanolic leaf extracts of Pimenta racemosa to transport the glucose into yeast cells. As a result, we found that the isopropanolic leaf extract of Pimenta racemosa have inhibitory activity against αamylase and also, which is efficient in glucose uptake. This therapeutic potentiality of Pimenta racemosa could be exploited in the treatment of Type 2 Diabetes mellitus. Further studies are also required to elucidate whether the plant have antidiabetic potential by in vivo for corroborating the traditional claim of the plant.

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