The Antibiosis Action and Rice-Induced Resistance, Mediated by a Lipopeptide from Bacillus amyloliquefaciens B014, in Controlling Rice Disease Caused by Xanthomonas oryzae pv. oryzae

Phosphite (PHI) has been used in the management of Phytophthora diseases since the 1970s.We assessed the effect of PHI on controlling the incidence of Xanthomonas oryzae pv.oryzae and Pyricularia grisea. As a result, PHI application significantly inhibited the incidence of the diseases. To clarify the molecular mechanism underlying this, a transcriptome study was employed. In total, 2064 differentially expressed genes (DEGs) were identified between control and PHI treatment. The key DEGs could be classified into phenylpropanoid biosynthesis (ko00940), starch and sucrose metabolism (ko00500), and plant hormone signal transduction (ko04075). The expressions of defense-related genes had a higher expression lever upon PHI treatment. This study provides new insights into the mechanism of protection effect of PHI against pathogens.

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Identification and characterization of genes frequently responsive to Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae infections in rice

BMC Genomics ◽

10.1186/s12864-019-6438-y ◽

2020 ◽

Vol 21 (1) ◽

Cited By ~ 1

Author(s):

Weiwen Kong ◽

Li Ding ◽

Xue Xia

Keyword(s):

Disease Resistance ◽

Magnaporthe Oryzae ◽

Enrichment Analysis ◽

Defense Responses ◽

Resistance Mechanisms ◽

Xanthomonas Oryzae ◽

Transcriptional Reprogramming ◽

Go Enrichment ◽

Phenylpropanoid Biosynthesis ◽

Rice Disease

Abstract Background Disease resistance is an important factor that impacts rice production. However, the mechanisms underlying rice disease resistance remain to be elucidated. Results Here, we show that a robust set of genes has been defined in rice response to the infections of Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae (Mor). We conducted a comprehensive analysis of the available microarray data from a variety of rice samples with inoculation of Xoo and Mor. A set of 12,932 genes was identified to be regulated by Xoo and another set of 2709 Mor-regulated genes was determined. GO enrichment analysis of the regulated genes by Xoo or Mor suggested mitochondrion may be an arena for the up-regulated genes and chloroplast be another for the down-regulated genes by Xoo or Mor. Cytokinin-related processes were most frequently repressed by Xoo, while processes relevant to jasmonic acid and abscisic acid were most frequently activated by Xoo and Mor. Among genes responsive to Xoo and Mor, defense responses and diverse signaling pathways were the most frequently enriched resistance mechanisms. InterPro annotation showed the zinc finger domain family, WRKY proteins, and Myb domain proteins were the most significant transcription factors regulated by Xoo and Mor. KEGG analysis demonstrated pathways including ‘phenylpropanoid biosynthesis’, ‘biosynthesis of antibiotics’, ‘phenylalanine metabolism’, and ‘biosynthesis of secondary metabolites’ were most frequently triggered by Xoo and Mor, whereas ‘circadian rhythm-plant’ was the most frequent pathway repressed by Xoo and Mor. Conclusions The genes identified here represent a robust set of genes responsive to the infections of Xoo and Mor, which provides an overview of transcriptional reprogramming during rice defense against Xoo and Mor infections. Our study would be helpful in understanding the mechanisms of rice disease resistance.

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Disease-reducing effects of aqueous leaf extract of Kalanchoe pinnata on rice bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae involve induced resistance

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2017.06.005 ◽

2017 ◽

Vol 100 ◽

pp. 57-66 ◽

Cited By ~ 3

Author(s):

Nguyễn Đắc Khoa ◽

Trương Văn Xạ ◽

Lâm Tấn Hào

Keyword(s):

Induced Resistance ◽

Leaf Extract ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Kalanchoe Pinnata ◽

Aqueous Leaf Extract

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Comparative Proteomic Analysis Reveals Novel Insights into the Interaction between Rice and Xanthomonas oryzae pv. oryzae

10.21203/rs.3.rs-41837/v3 ◽

2020 ◽

Author(s):

Fan Zhang ◽

Liyu Huang ◽

Dan Zeng ◽

Casiana Vera Cruz ◽

...

Keyword(s):

Introgression Line ◽

Defense Responses ◽

Xanthomonas Oryzae ◽

Recurrent Parent ◽

Compatible Interaction ◽

Transcription Activator ◽

Incompatible Interaction ◽

Pathogen Virulence ◽

Pathogen Invasion ◽

Rice Disease

Abstract Background: Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Rice introgression line H471, derived from the recurrent parent Huang-Hua-Zhan (HHZ) and the donor parent PSBRC28, exhibits broad-spectrum resistance to Xoo, including to the highly virulent Xoo strain PXO99A, whereas its parents are susceptible to PXO99A. To characterize the responses to Xoo, we compared the proteome profiles of the host and pathogen in the incompatible interaction (H471 inoculated with PXO99A) and the compatible interaction (HHZ inoculated with PXO99A).Results: In this study, a total of 374 rice differentially abundant proteins (DAPs) and 117 Xoo DAPs were detected in the comparison between H471 + PXO99A and HHZ + PXO99A. Most of the Xoo DAPs related to pathogen virulence, including the outer member proteins, type III secretion system proteins, TonB-dependent receptors, and transcription activator-like effectors, were less abundant in the incompatible interaction than in the compatible interaction. The rice DAPs were mainly involved in secondary metabolic processes, including phenylalanine metabolism and the biosynthesis of flavonoids and phenylpropanoids. Additionally, some DAPs involved in the phenolic phytoalexin and salicylic acid (SA) biosynthetic pathways accumulated much more in H471 than in HHZ after the inoculation with PXO99A, suggesting that phytoalexin and SA production was induced faster in H471 than in HHZ. Further analyses revealed that the SA content increased much more rapidly in H471 than in HHZ after the inoculation, suggesting that the SA signaling pathway was activated faster in the incompatible interaction than in the compatible interaction.Conclusions: Overall, our results indicate that during an incompatible interaction between H471 and PXO99A, rice plants prevent pathogen invasion and also initiate multi-component defense responses that inhibit disease development.

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Genome-Wide Association Study of QTLs Conferring Resistance to Bacterial Leaf Streak in Rice

Plants ◽

10.3390/plants10102039 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2039

Author(s):

Xiaofang Xie ◽

Yan Zheng ◽

Libin Lu ◽

Jiazheng Yuan ◽

Jie Hu ◽

...

Keyword(s):

Genome Wide Association Study ◽

Bacterial Pathogen ◽

Xanthomonas Oryzae ◽

Phenotypic Variance ◽

Bacterial Leaf Streak ◽

Chromosome 11 ◽

Severe Damage ◽

Genome Wide ◽

Rice Disease ◽

Depth Study

Bacterial leaf streak (BLS) is a devastating rice disease caused by the bacterial pathogen, Xanthomonas oryzae pv. oryzicola (Xoc), which can result in severe damage to rice production worldwide. Based on a total of 510 rice accessions, trialed in two seasons and using six different multi-locus GWAS methods (mrMLM, ISIS EM-BLASSO, pLARmEB, FASTmrMLM, FASTmrEMMA and pKWmEB), 79 quantitative trait nucleotides (QTNs) reflecting 69 QTLs for BLS resistance were identified (LOD > 3). The QTNs were distributed on all chromosomes, with the most distributed on chromosome 11, followed by chromosomes 1 and 5. Each QTN had an additive effect of 0.20 (cm) and explained, on average, 2.44% of the phenotypic variance, varying from 0.00–0.92 (cm) and from 0.00–9.86%, respectively. Twenty-five QTNs were detected by at least two methods. Among them, qnBLS11.17 was detected by as many as five methods. Most of the QTNs showed a significant interaction with their environment, but no QTNs were detected in both seasons. By defining the QTL range for each QTN according to the LD half-decay distance, a total of 848 candidate genes were found for nine top QTNs. Among them, more than 10% were annotated to be related to biotic stress resistance, and five showed a significant response to Xoc infection. Our results could facilitate the in-depth study and marker-assisted improvement of rice resistance to BLS.

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Comparative proteomic analysis reveals novel insights into the interaction between rice and Xanthomonas oryzae pv. oryzae

BMC Plant Biology ◽

10.1186/s12870-020-02769-7 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Fan Zhang ◽

Liyu Huang ◽

Dan Zeng ◽

Casiana Vera Cruz ◽

...

Keyword(s):

Introgression Line ◽

Defense Responses ◽

Xanthomonas Oryzae ◽

Recurrent Parent ◽

Compatible Interaction ◽

Transcription Activator ◽

Incompatible Interaction ◽

Pathogen Virulence ◽

Pathogen Invasion ◽

Rice Disease

Abstract Background Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Rice introgression line H471, derived from the recurrent parent Huang-Hua-Zhan (HHZ) and the donor parent PSBRC28, exhibits broad-spectrum resistance to Xoo, including to the highly virulent Xoo strain PXO99A, whereas its parents are susceptible to PXO99A. To characterize the responses to Xoo, we compared the proteome profiles of the host and pathogen in the incompatible interaction (H471 inoculated with PXO99A) and the compatible interaction (HHZ inoculated with PXO99A). Results In this study, a total of 374 rice differentially abundant proteins (DAPs) and 117 Xoo DAPs were detected in the comparison between H471 + PXO99A and HHZ + PXO99A. Most of the Xoo DAPs related to pathogen virulence, including the outer member proteins, type III secretion system proteins, TonB-dependent receptors, and transcription activator-like effectors, were less abundant in the incompatible interaction than in the compatible interaction. The rice DAPs were mainly involved in secondary metabolic processes, including phenylalanine metabolism and the biosynthesis of flavonoids and phenylpropanoids. Additionally, some DAPs involved in the phenolic phytoalexin and salicylic acid (SA) biosynthetic pathways accumulated much more in H471 than in HHZ after the inoculation with PXO99A, suggesting that phytoalexin and SA productions were induced faster in H471 than in HHZ. Further analyses revealed that the SA content increased much more rapidly in H471 than in HHZ after the inoculation, suggesting that the SA signaling pathway was activated faster in the incompatible interaction than in the compatible interaction. Conclusions Overall, our results indicate that during an incompatible interaction between H471 and PXO99A, rice plants prevent pathogen invasion and also initiate multi-component defense responses that inhibit disease development.

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Comparative Proteomic Analysis Reveals Novel Insights into the Interaction between Rice and Xanthomonas oryzae pv. oryzae

10.21203/rs.3.rs-41837/v1 ◽

2020 ◽

Author(s):

Fan Zhang ◽

Liyu Huang ◽

Dan Zeng ◽

Casiana Vera Cruz ◽

...

Keyword(s):

Introgression Line ◽

Defense Responses ◽

Xanthomonas Oryzae ◽

Recurrent Parent ◽

Compatible Interaction ◽

Transcription Activator ◽

Incompatible Interaction ◽

Pathogen Virulence ◽

Pathogen Invasion ◽

Rice Disease

Abstract Background: Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Rice introgression line H471, derived from the recurrent parent Huang-Hua-Zhan (HHZ) and the donor parent PSBRC28, exhibits broad-spectrum resistance to Xoo, including to the highly virulent Xoo strain PXO99A, whereas its parents are susceptible to PXO99A. To characterize the responses to Xoo, we compared the proteome profiles of the host and pathogen in the incompatible interaction (H471 inoculated with PXO99A) and the compatible interaction (HHZ inoculated with PXO99A).Results: In this study, a total of 374 rice differentially abundant proteins (DAPs) and 117 Xoo DAPs were detected in the comparison between H471 + PXO99A and HHZ + PXO99A. Most of the Xoo DAPs related to pathogen virulence, including the outer member proteins, type III secretion system proteins, TonB-dependent receptors, and transcription activator-like effectors, were less abundant in the incompatible interaction than in the compatible interaction. The rice DAPs were mainly involved in secondary metabolic processes, including phenylalanine metabolism and the biosynthesis of flavonoids and phenylpropanoids. Additionally, some DAPs involved in the phenolic phytoalexin and salicylic acid (SA) biosynthetic pathways accumulated much more in H471 than in HHZ after the inoculation with PXO99A, suggesting that phytoalexin and SA production was induced faster in H471 than in HHZ.Conclusions: Overall, our results indicate that during an incompatible interaction between H471 and PXO99A, rice plants prevent pathogen invasion and also initiate multi-component defense responses that inhibit disease development.

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Ketahanan Berbagai Kultivar Padi Lokal terhadap Penyakit Hawar Daun Bakteri

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.12.3.89 ◽

2016 ◽

Vol 12 (3) ◽

pp. 89

Author(s):

Andi Khaeruni ◽

Erwin Najamuddin ◽

Teguh Wijayanto ◽

Syair Syair

Keyword(s):

Control Strategies ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Rice Cultivars ◽

Rice Varieties ◽

Resistant Varieties ◽

Rice Disease ◽

Generative Phase ◽

Blight Disease ◽

Screen House

Bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae is an important rice disease in Indonesia, including in South East Sulawesi. The use of resistant varieties is one of the effective and environmentally friendly control strategies to suppress the disease. This study aimed to determine the level of resistance of some local rice varieties of South and Southeast Sulawesi against Xanthomonas oryzae pv. oryzae pathotypes IV, VIII and X. The study was conducted in a screen house involving 3 bacterial pathotypes and 11 local rice cultivars. Shearing method was used for inoculation of bacteria to leaf on vegetative and generative phases. Disease incidences were measured 3 weeks after inoculation, and the data was further used to determine the level of resistance of the tested rice cultivars. The results showed that incubation period of the disease was longer on Kelaca cultivar compared to other cultivars. On vegetative phase, this cultivar showed moderate resistant to pathotypes IV and X, and highly resistant to patotype VIII, whereas on the generative phase it showed moderate resistant to pathotypes IV and VII, and highly resistant to pathotype X. Therefore, Kelaca cultivar can be recommended for endemic areas of leaf blight in South and Southeast Sulawesi

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