scholarly journals Expression of genes related to soil flooding tolerance in soybeans

2019 ◽  
Vol 41 ◽  
pp. e42709
Author(s):  
Gabriele Casarotto ◽  
Tiago Edu Kaspary ◽  
Luan Cutti ◽  
André Luis Thomas ◽  
Jose Fernandes Barbosa Neto
Keyword(s):  
Energy Metabolism ◽  
Structural Changes ◽  
Reducing Power ◽  
Redox Status ◽  
Development Stage ◽  
Xyloglucan Endotransglycosylase ◽  
Relative Expression ◽  
Soil Flooding ◽  
Expression Of Genes ◽  
Soybean Plants

The flooded environment brings about injuries to soybeans that vary depending on the adaptation ability of the genotype. Oxygen deprivation promotes the induction of the expression of genes related to glycolysis and fermentation pathways to maintain energy metabolism and, in addition to reducing-power consuming processes, act in the formation of adaptive structures and the maintenance of the redox status of the plant. The aim of this work was to evaluate the relative expression of genes related to soil flooding response in two contrasting soybean cultivars. Soybean plants of the sensitive (BRS 154) and tolerant (I27) cultivars at the V1 development stage were submitted to the flooding and control conditions (without flooding) for 0, 24, 48, and 96 hours. The relative expression of genes associated with flooding, including enolase (ENO), alcohol dehydrogenase 1 (ADH1), alanine aminotransferase 2 (ALAT2), hemoglobin 1 (GLB1), LOB41 domain-containing protein (LBD41), xyloglucan endotransglycosylase (XETP) and ascorbate peroxidase (APX2), was evaluated by means of RT-qPCR. The relative expression, in general, increased with flooding, especially in the root tissue. Cultivar I27 responded positively as observed by the expression of the maintenance genes of energy metabolism, structural changes and detoxification, suggesting the presence of three tolerance mechanisms in the flooding response.

scholarly journals Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohamed S. Sheteiwy ◽  
Dina Fathi Ismail Ali ◽  
You-Cai Xiong ◽  
Marian Brestic ◽  
Milan Skalicky ◽  
...  
Keyword(s):  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Secondary Metabolism ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Relative Expression ◽  
Reverse Trend ◽  
Soybean Plants

Abstract Background The present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5). Results Highest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress. Conclusions The results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress.

Protective effects of lipoic acid against acrylamide-induced neurotoxicity: involvement of mitochondrial energy metabolism and autophagy

2017 ◽  
Vol 8 (12) ◽  
pp. 4657-4667 ◽  
Author(s):  
Ge Song ◽  
Zhigang Liu ◽  
Luanfeng Wang ◽  
Renjie Shi ◽  
Chuanqi Chu ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Lipoic Acid ◽  
Redox Status ◽  
Protective Effects ◽  
Mitochondrial Energy Metabolism

Lipoic acid (LA) suppressed acrylamide (ACR)-induced inflammation, redox status disturbance, autophagy, and apoptosis mediated by mitochondria in the SH-SY5Y cells.

scholarly journals Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro

2011 ◽  
Vol 40 (1) ◽  
pp. 124-128
Author(s):  
Sabine Wohlres-Viana ◽  
Mariana Cortes Boite ◽  
João Henrique Moreira Viana ◽  
Marco Antonio Machado ◽  
Luiz Sérgio de Almeida Camargo
Keyword(s):  
Culture System ◽  
Rna Extraction ◽  
Endogenous Control ◽  
Bovine Embryos ◽  
Relative Expression ◽  
Gapdh Gene ◽  
Expression Of Genes ◽  
In Vitro Culture System

The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase α1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase β2 transcripts 0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase β2 genes, however, it affects expression of Na/K-ATPase α1.

scholarly journals The Effect of Different Programs of Exercise on The Expression of Genes Associated with Endurance and Energy Metabolism

2021 ◽  
pp. 482-490
Author(s):  
Neşe AKPINAR KOCAKULAK ◽  
Serpil TAHERİ ◽  
Elif Funda ŞENER ◽  
Kenan AYCAN ◽  
Yusuf ÖZKUL
Keyword(s):  
Energy Metabolism ◽  
Expression Of Genes

Solanine Interferes With AKT/p-AKT and PI3K/ p-PI3K to Inhibit HIF and Destroy Cell Energy Metabolism

2021 ◽  
Author(s):  
Peng Wang ◽  
Yidong Wang
Keyword(s):  
Energy Metabolism ◽  
Renal Cancer ◽  
Downward Trend ◽  
Akt Pathway ◽  
Control Group ◽  
Specific Method ◽  
Elisa Method ◽  
Relative Expression ◽  
Low Concentrations ◽  
Cell Energy

Abstract The purpose of this study was to explore the mechanism of Solanine disrupting energy metabolism in human renal cancer ACHN cells and to clarify its target. The specific method was to culture human renal cancer ACHN cell lines, and to intervene with Solanine of high, medium and low concentrations. The content of ATP in cells was measured by ELISA method. The expression of HIF-1α protein and the expression of PI3K, AKT, p-PI3K, p-AKT in PI3K/ AKT pathway were detected by Western blotting. The results showed that compared with the control group, the relative expression of p-PI3K and p-AKT showed a downward trend with the increase of Solanine concentration (P < 0.05), while the relative expression of PI3K and AKT showed no significant change (P > 0.05). In addition, the relative expression of HIF-1α also showed a downward trend (P < 0.05). According to the above results, it is suggested that Solanine can significantly inhibit the energy metabolism of renal cancer cells, the main mechanism of which is the down-regulation of HI-1αf downstream of the PI3K/Akt pathway by inhibiting the phosphorylation process of PI3K/ p-PI3K and Akt/p-Akt.

Investigation the Relative Expression of Genes of ATPase Family in Major and Regular Depressed Patients

2016 ◽  
Vol 3 (4) ◽  
Author(s):  
Sima Farzaneh ◽  
Tavkol Moussazadeh ◽  
Mohammad Zaefizadeh
Keyword(s):  
Quantitative Measurement ◽  
Comparison Group ◽  
Rt Pcr ◽  
Statistical Population ◽  
Relative Expression ◽  
Depressed Patients ◽  
Normal People ◽  
Expression Of Genes ◽  
Significant Difference ◽  
Comprehensive Manner

To develop new pharmaceutical strategies in a comprehensive manner, it is necessary to understand the reasons for major depression. Present paper aims for comparison of expression of ATPase families in major depressed and normal people. Statistical population of this research includes all depressed people coming to professional clinic of Shahryar in 2014-15. This contains 47 depressed patients which were selected based on accessibility from those visiting clinic. 26 normal people were selected as comparison group as well. To study the relative expression of ATPase1-3, quantitative measurement method using Real-Time RT-PCR was used. Relative expression of ATPase2 showed a significant reduction compared to normal people (p<0.02). In patients, ATPase1 had higher relative expression compared to normal people. However, ATPase had no significant difference among patients and normal people. Results demonstrate that ATPase1 and ATPase2 are important variables in depression but ATPase3 plays no pivotal role in this regard.

scholarly journals Basal anti-inflammatory responses in cultured endothelial cells exposed to two conjugated linoleic acids

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Carina Valenzuela ◽  
Elizabeth Miles ◽  
Philip Calder
Keyword(s):  
Inflammatory Responses ◽  
Adhesion Assay ◽  
Dependent Manner ◽  
Relative Expression ◽  
Inflammatory Pathways ◽  
Expression Of Genes ◽  
Low Concentrations ◽  
Genes Encoding ◽  
Anti Inflammatory ◽  
Pre Treatment

AbstractConjugated linoleic acid (CLA) isomers have been shown to possess anti-atherosclerotic properties, which may be related to the downregulation of inflammatory pathways. Whether low concentrations of CLAs are able to affect basal, unstimulated endothelial cell (EC) responses is not clear. The aim of this study was to evaluate the effects of two CLAs (cis-9, trans-11 and trans-10, cis-12) on basal inflammatory responses by ECs.EA.hy926 cells (HUVEC lineage) were cultured under standard conditions and exposed to CLAs (1 and 10 μM) for 48 hours. MTT assay was performed to determine cell viability; incorporation of FA was confirmed by gas chromatography; inflammatory mediators were assessed by multiplex immunoassay; the relative expression of genes encoding transcription factors and inflammatory cytokines was assessed through real-time PCR and static adhesion assay was used to evaluate monocyte attachment to the EC monolayer.CLAs were incorporated into ECs in a dose-dependent manner. Pre-treatment with CLA9,11 (1 uM) significantly reduced unstimulated, basal concentrations of MCP-1 (p < 0.05), and CLA10,12 at 10 uM had the same effect (p < 0.05). Both CLAs at 10 uM increased the relative expression of NFκβ (p < 0.01 and p < 0.05, respectively), while decreasing the relative expression of PPARα (p < 0.0001), COX-2 (p < 0.0001) and IL-6 (p < 0.0001). In contrast, no effect was observed in the adhesion assay for either CLA.These results suggest that both CLAs at a low concentration have a neutral or modest anti-inflammatory effect in basal conditions, which may influence endothelial function and risk of vascular disease. Interestingly, at these low CLA concentrations some pro-inflammatory genes are upregulated while others are down regulated. This suggests complex effects of CLAs on inflammatory pathways.

Disrupted expression of genes essential for skeletal muscle fibre integrity and energy metabolism in Vitamin D deficient rats

2020 ◽  
Vol 197 ◽  
pp. 105525 ◽  
Author(s):  
Ramesh Gogulothu ◽  
Devika Nagar ◽  
Srividya Gopalakrishnan ◽  
Venkat R. Garlapati ◽  
Prathap R. Kallamadi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Energy Metabolism ◽  
Muscle Fibre ◽  
Skeletal Muscle Fibre ◽  
Expression Of Genes

scholarly journals O2 Dependence of in vivo Brain Cytochrome Redox Responses and Energy Metabolism in Bloodless Rats

1988 ◽  
Vol 8 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Avis L. Sylvia ◽  
Claude A. Piantadosi
Keyword(s):  
Energy Metabolism ◽  
Direct Evidence ◽  
Redox Status ◽  
Energy Deficit ◽  
Brain Oxygenation ◽  
Control Value ◽  
Partial Pressures ◽  
Fluorocarbon Emulsion

Oxygen-dependent changes in brain cytochrome redox state and cerebrocortical energy metabolism were evaluated in fluorocarbon-circulated rats at hematocrits of <1%. Redox levels of three respiratory chain cytochrome complexes, b, c, and a,a3 (cytochrome c oxidase), were continuously measured directly through the intact skulls of animals using reflectance spectrophotometry. The in vivo redox status of cytochromes at different Fio2 was directly compared with in vitro measured changes in cortical metabolites known to reflect energy production, i.e., glucose, pyruvate, lactate, phosphocreatine (PCr), ADP, and ATP. Lowering the Fio2 to <1.0 caused the cytochromes to become increasingly more reduced. This was associated with increased tissue accumulation of pyruvate and lactate and a concomitant increase in the lactate/pyruvate (L/P) ratio. At Fio2 = 0.6, cytochromes b, c, and a,a3 were 57, 53, and 46% reduced, respectively. There was no apparent cerebral energy deficit since changes in cortical PCr, ADP, and ATP concentrations were not statistically significant. Bloodless animals did not survive below Fio2 = 0.5. At this Fio2, the inability of the animals to sustain arterial pressure correlated ( r = 0.87) with depletion of PCr and further increases in the L/P ratio ( r = 0.66). Yet, the cortical ATP content was reduced by only 9% of control value. These data provide direct evidence that fluorocarbon emulsion (FC-43) sustains brain oxygenation and energy metabolism at high partial pressures of molecular o2. At lower Fio2, however, mitochondrial o2 uptake becomes limited as a function of decreasing perfusion pressure.

Sign in / Sign up

Export Citation Format

Share Document