Effects of Nitrate Concentration on Heterotrophic Denitrification in Wastewater Using Poly (Butylene Succinate) as a Carbon Source and Carrier

2014 ◽  
Vol 665 ◽  
pp. 469-478
Author(s):  
Guo Zhi Luo ◽  
Wen Jing Sun ◽  
Qian Liu ◽  
Yu Hu ◽  
Hong Xin Tan

Biodegradable polymer pellets (BDPs) can act as biofilm carrier and as water insoluble carbon source for denitrification simultaneously, which is accessible only by enzymatic attack. It is expected that organic carbon source for heterotrophic denitrification by using BDPs will not be overdose or shortage. The current batch experiment was conducted to examine if the PBS would supply enough carbon source to the denitrification with high concentration nitrate. The initial nitrate nitrogen (NO3--N) concentrations were 100 mg NO3-N/l (T1), 300 mg NO3-N/l (T2) and 500 mg NO3-N/l (T3) respectively. The results showed that the initial nitrate concentrations have significant effects on the removal of nitrate nitrogen and total nitrogen (TN) using PBS as carbon source. The efficiencies of removal of nitrate and TN in T1 were almost 100%. The amounts of NO3--N and TN that were removed in T2 were 286.60±6.66 mg NO3-N/g PBS (in dry wt), which was significantly higher than that of T1 and T3. Accumulation of ammonium and nitrite were observed in T2 and T3. The morphological changes and the weight losses observed for PBS granules indicated that good degradation occurred in static denitrification environments. But the insufficient of carbon sources for denitrification in T2 and T3 was observed.

2015 ◽  
Vol 71 (7) ◽  
pp. 1019-1025 ◽  
Author(s):  
Chuanfu Wu ◽  
Danqi Tang ◽  
Qunhui Wang ◽  
Juan Wang ◽  
Jianguo Liu ◽  
...  

A suitable carbon source is significant for biological nitrate removal from groundwater. In this study, slow-release carbon sources containing polylactic acid (PLA) and starch at 8:2, 7:3, 6:4, 5:5, 4:6, and 3:7 ratios were prepared using a blending and fusing technique. The PLA/starch blend was then used as a solid carbon source for biological nitrate removal. The carbon release rate of PLA/starch was found to increase with increased starch content in leaching experiments. PLA/starch at 5:5 mass ratio was found to have the highest denitrification performance and organic carbon consumption efficiency in semi-continuous denitrification experiments, and was also revealed to support complete denitrification at 50 mg-N/L influent nitrate concentration in continuous experiments. The effluent nitrate concentration was <2 mg NO3–-N/L, which met the national standard (GB 14848-93) for groundwater. Scanning electron microscopy results further showed that the surface roughness of PLA/starch increased with prolonged experimental time, which may be conducive to microorganism attachment. Therefore, PLA/starch was a suitable carbon source and biofilm carrier for groundwater remediation.


2013 ◽  
Vol 316-317 ◽  
pp. 625-628
Author(s):  
Jian Mei Zhang ◽  
Chuan Ping Feng ◽  
Si Qi Hong ◽  
Hui Ling Hao

The method of the heterotrophic denitrification remediation of nitrate-polluted groundwater involves the study of organic carbon sources as electron donor. The aim of the present study was to evaluate wheat straw for its ability to enhance denitrification in column experiments. The inlet concentration was 50.0 mgNO3--N/L and the column operated at the flow rate of 2.0 ml/min. The result showed that in the presence of wheat straw, highly reducing conditions were generated and complete removal of nitrate (>95%) was achieved, with less accumulation of nitrite. Consequently, wheat straw is an attractive carbon source for groundwater denitrification.


2012 ◽  
Vol 65 (7) ◽  
pp. 1238-1243 ◽  
Author(s):  
Guochao Li ◽  
Jie Chen ◽  
Tao Yang ◽  
Jianqi Sun ◽  
Shenglu Yu

In this research the agricultural by-product corncob was investigated as a carbon source as well as a biofilm carrier to remove organic matter, expressed as chemical oxygen demand (COD) and nitrate nitrogen (nitrate-N), from wastewater in a batch laboratory reactor. The performance of a reactor with corncob as the carbon source and the biofilm carrier was compared with a control batch reactor with suspended plastic carriers and glucose as the sole carbon source. With 60 vol% of corncob carriers inside the reactor, a soluble COD/N ratio of 4.2 g COD g N−1 was enough for total denitrification, nearly half of the control reactor (9.5 g COD g N−1), at 23 h reaction time. The specific denitrification rate decreased with increasing soluble COD consumption for both reactors. Nitrate and COD removal efficiencies decreased with shorter retention times, with accentuated effects in the reactor. This study suggested corncob as a feasible carbon source and that reaction time was a limiting factor with corncob used as the carbon source for denitrification.


1998 ◽  
Vol 38 (6) ◽  
pp. 237-243 ◽  
Author(s):  
A. Mohseni-Bandpi ◽  
D. J. Elliott

A pilot scale rotating biological contactor (RBC) was used to investigate the removal of nitrate-nitrogen from groundwater using three different carbon sources, i.e., methanol, ethanol and acetic acid. Optimum carbon sources to influent nitrate-nitrogen ratio were established by varying the influent concentration of carbon sources. The optimum ratio of methanol, ethanol and acetic acid to nitrate-nitrogen ratios were found to be 2.9, 2.35 and 4.3 respectively. The nitrate-nitrogen removal efficiency averaged 93, 91 and 98 for methanol, ethanol and acetic acid respectively at a loading rate of 76 mg/m2.h. The results of this study show that the acetic acid is the most efficient carbon source for removal of nitrate-nitrogen. Effluent nitrite-nitrogen concentration was minimum for acetic acid as compared with ethanol and methanol. The effluent contained minimum suspended solids and turbidity for methanol as a carbon source. The results of this study indicate that biological nitrate removal using a RBC is a reliable and stable system under all the three carbon sources. The denitrified water in all cases requires some post treatment to oxidise the residual carbon source and remove biomass before distribution.


2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Manxi Liu ◽  
Lu Xia ◽  
Ruinan Liu ◽  
Zongjun Gao ◽  
Cong Han ◽  
...  

To investigate effective and reasonable methods for the remediation of nitrate nitrogen pollution in groundwater, two groups of laboratory denitrification experiments were conducted: one on the effect of native denitrifying microbes in groundwater and another on the effect of artificially added denitrifying microbes. The water used in the experiment was typical groundwater with a high concentration of nitrate nitrogen. The temperature was controlled at 15°C. Both groups of experiments established four types of culture environments: anaerobic, anaerobic with an added carbon source (glucose), aerobic, and aerobic with an added carbon source (glucose). The results indicated that native denitrifying microbes in the groundwater have almost no ability to remove high concentrations of nitrate nitrogen. However, artificially added denitrifying microbes can effectively promote denitrification. Artificially added denitrifying microbes had the highest activity in an anaerobic environment in which a carbon source had been added, and the rate removal of a high concentration of nitrate nitrogen in groundwater was the highest and reached as high as 89.52%.


2006 ◽  
Vol 53 (6) ◽  
pp. 175-180 ◽  
Author(s):  
S. Yan ◽  
R.D. Tyagi ◽  
R.Y. Surampalli

Activated sludge from different full-scale wastewater treatment plants (municipal, pulp and paper industry, starch manufacturing and cheese manufacturing wastewaters) was used as a source of microorganisms to produce biodegradable plastics in shake flask experiments. Acetate, glucose and different wastewaters were used as carbon sources. Pulp and paper wastewater sludge was found to accumulate maximum concentration (43% of dry weight of suspended solids) of polyhydroxy alkanoates (PHA) with acetate as carbon source. Among the different wastewaters tested as a source of carbon, pulp and paper industry and starch industry wastewaters were found to be the best source of carbon while employing pulp and paper activated sludge for maximum accumulation of PHA. High concentration of volatile fatty acids in these wastewaters was the probable reason.


2016 ◽  
Vol 216 ◽  
pp. 1004-1013 ◽  
Author(s):  
Yun-Jie Ruan ◽  
Ya-Le Deng ◽  
Xi-Shan Guo ◽  
Michael B. Timmons ◽  
Hui-Feng Lu ◽  
...  

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