Preparation and Characterization of Vitamin a Palmitate-Loaded Nanostructured Lipid Carriers as Delivery Systems for Food Products

2011 ◽  
Vol 236-238 ◽  
pp. 1818-1823 ◽  
Author(s):  
Rong Kong ◽  
Qiang Xia ◽  
Guang Yu Liu
Keyword(s):  
Vitamin A ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Food Products ◽  
Storage Conditions ◽  
Correlation Spectroscopy ◽  
Nanostructured Lipid Carriers ◽  
Laser Diffractometry ◽  
Physical And Chemical

Vitamin A Palmitate-loaded Nanostructured Lipid Carriers were prepared by High Pressure Homogenization (HPH) technique. The influences of storage conditions such as time and temperature on the physical and chemical storage stability of VAP-NLCs were studied in details. Preparation and physicochemical properties of VAP lipid nanoparticles were investigated using various analytical equipments such as TEM, photon correlation spectroscopy (PCS), laser diffractometry (LD) and ultraviolet spectrophotometer. The respective optimal HPH pressure and cycles were 70MPa and 5 cycles, so VAP-NLC dispersions under the optimized condition gave rise to the entrapment efficiency (EE) of 99.8%, PI of 0.234, mean diameter of 144±1.7 nm (2% VAP loading capacity). In addition, VAP lipid nanoparticle has a distinct spherical shape, its particle sizes were around 60–330 nm according to VAP contents and zeta potential values -18~-23 mV. Furthermore, the pH of VAP-NLC dispersion was near to 7.0. This study showed that VAP-NLC could hopefully be applied for food products.

Preparation and Characterization of Coenzyme Q10-Loaded Nanostructured Lipid Carriers

2011 ◽  
Vol 694 ◽  
pp. 170-174
Author(s):  
Qiang Xia ◽  
Jia Ying Wu
Keyword(s):  
Coenzyme Q ◽  
Correlation Spectroscopy ◽  
Nanostructured Lipid Carriers ◽  
Photon Correlation ◽  
Vis Spectroscopy ◽  
Before And After ◽  
Size Growth ◽  
The Mean ◽  
Stability Results

The enhancement of stability of light sensitive CoQ10 was achieved by preparation of coenzyme Q10-loaded Nanostructured Lipid Carriers through High Pressure Homogenization (HPH). Well-dissolved lipids of CoQ10 were selected, optimized ratio of emusifiers and lipids were determined for the formulation. Obtained by photon correlation spectroscopy (PCS), the mean particle size of CoQ10-NLC was 112 ± 7 nm within 60 days after preparation. In terms of centrifugal stability, results of laser diffraction (LD) analysis eliminated the existence of aggregated particles with micrometers and showed almost no size growth before and after centrifugation. Zeta potential values were from -50 to -55 mV with pH in the range of 6.56–6.72. The concentration of CoQ10-NLC measured by UV-Vis spectroscopy was as high as 8.13 mg/mL.

scholarly journals Chemical and Physical Characterization of Peanut Powder Extracts

2018 ◽  
Vol 10 (4) ◽  
pp. 323
Author(s):  
Luzia Marcia de Melo Silva ◽  
Francisco de Assis Cardoso Almeida ◽  
Francinalva Cordeiro de Sousa ◽  
Deise Souza de Castro ◽  
Inácia dos Santos Moreira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ambient Temperature ◽  
Spherical Shape ◽  
Chemical Compounds ◽  
Growth Potential ◽  
Chemical Characteristics ◽  
Peanut Extract ◽  
Physical And Chemical ◽  
Great Growth

The production of lyophilized foods is a market with great growth potential, for providing important preservation characteristics, such as stability at ambient temperature, versatility of the product and preservation of the chemical compounds. Given the functional effects of peanut powder extracts, this study aimed to quantify the bioactive compounds and determine physical and chemical characteristics, comparing samples with and without skin. After obtaining the aqueous peanut extract the samples were frozen at -18 °C for 24 h. The formulated extracts were dried in a benchtop lyophilizer operating at temperature of -55 °C for a period of 48 hours. The powder extracts were disintegrated in a multiprocessor for 30 seconds and the samples were physically and chemically evaluated. The powder extracts were classified as non-hygroscopic, exhibiting poor fluidity and intermediate cohesiveness in samples with skin, and high cohesiveness in samples without skin. The powders showed agglomerated particles, with irregular and non-uniform shape. Potassium was the mineral found in largest amounts, as well as oleic and linoleic fatty acids. The particles of the powders exhibit a spherical shape, showing the presence of amorphous surfaces, in which there is no repetition of geometric forms. The peanut powder extracts are classified as non-hygroscopic, have poor fluidity, intermediate cohesiveness in samples with skin and high cohesiveness in samples without skin.

scholarly journals Preparation and Characterisation of Nobiletin-Loaded Nanostructured Lipid Carriers

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Wei Huang ◽  
Huating Dou ◽  
Houjiu Wu ◽  
Zhigao Sun ◽  
Hua Wang ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Chemical Interaction ◽  
Amorphous State ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carriers ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Lipid Mixture ◽  
Food Response

The objective of this manuscript was to investigate and optimise the potential of nanostructured lipid carriers (NLCs) as a carrier system for nobiletin (NOB), which was prepared by high-pressure homogenisation method. Additionally, this study was focused on the application of NOB-loaded NLC (NOB-NLC) in functional food. Response surface method with a three-level Box–Behnken design was validated through analysis of variance, and the robustness of the design was confirmed through the correspondence between the values measured in the experiments and the predicted ones. Properties of the prepared NOB-NLC, such as Z-average, polydispersity, entrapment efficiency, zeta potential, morphology, and crystallinity, were investigated. NOB-NLC exhibited a spherical shape with a diameter of 112.27 ± 5.33 nm, zeta potential of −35.1 ± 2.94 mV, a polydispersity index of 0.251 ± 0.058, and an EE of 81.06%  ±  6.02%. Results from X-ray diffraction and differential scanning calorimetry of NOB-NLC reviewed that the NOB crystal might be converted to an amorphous state. Fourier transform infrared spectroscopic analysis demonstrated that chemical interaction was absent between the compound and lipid mixture in NOB-NLC.

Extraction and characterization of the blue-sensitive visual pigment of the toad Bufo marinus

1987 ◽  
Vol 65 (4) ◽  
pp. 884-887 ◽  
Author(s):  
A. J. Sillman
Keyword(s):  
Vitamin A ◽  
Visual Pigment ◽  
Chemical Properties ◽  
Bufo Marinus ◽  
Physical And Chemical Properties ◽  
Electrophysiological Studies ◽  
Physical And Chemical ◽  
Toad Bufo ◽  
And Chemical Properties

The blue-sensitive visual pigment of the green rods of Bufo marinus was extracted with digitonin. The pigment is present in an amount equal to about 11% that of rhodopsin. It is based on vitamin A1 and exhibits a maximum absorbance of 433 nm. The pigment is labile and readily destroyed by hydroxylamine, regenerates to a much greater degree than does rhodopsin, and is more effectively extracted from the membrane than is rhodopsin. The green rod pigment of Bufo marinus appears to share the same physical and chemical properties as the green rod pigments of other amphibians. Therefore, the results of electrophysiological studies on the green rods of Bufo marinus can be more confidently generalized to other species. The results are discussed in terms of the blue phototaxis that is characteristic of many amphibians.

Preparation and Characterization of Azithromycin-Loaded Nanostructured Lipid Carriers

2011 ◽  
Vol 236-238 ◽  
pp. 2917-2920 ◽  
Author(s):  
Jia Ying Wu ◽  
Qiang Xia
Keyword(s):  
Particle Size ◽  
Photon Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Nanostructured Lipid Carriers ◽  
High Pressure Homogenization ◽  
Photon Correlation ◽  
Mean Particle Size ◽  
Ophthalmic Preparation ◽  
The Mean

To function lipophilic drug Azithromycin (AZM) as ophthalmic preparation, Azithromycin-loaded Nanostructured Lipid Carriers (AZM-NLC) were prepared by High Pressure Homogenization (HPH), and its stability was analyzed afterwards. Well-dissolved lipids of Azithromycin were selected, how the ratio of surfactants to lipids influenced the preparation of AZM-NLC was studied, and mature surfactant system was chosen. The optimized ratio of lipid carriers to emulsifiers [ polyoxyethylene (40) stearate - Poloxamer188 (7:3)] in this study was 1:6, and the final drug-capacity rate was 0.5%. Obtained by photon correlation spectroscopy, the mean particle size of AZM-NLC was 76±2 nm. The results showed that AZM-NLC had kept stable for 15 days, and mean particle size increased to 83 nm after four months.

Freeze-Drying and Characterization of Vitamin a Palmitate-Loaded Nanostructured Lipid Carriers (NLC)

2011 ◽  
Vol 694 ◽  
pp. 365-369 ◽  
Author(s):  
Qiang Xia ◽  
Rong Kong
Keyword(s):  
Vitamin A ◽  
Oral Delivery ◽  
Food Industry ◽  
Freeze Drying ◽  
Nanostructured Lipid Carriers ◽  
Water Soluble ◽  
Drying Process ◽  
Poorly Water Soluble ◽  
Promising Perspective

Vitamin A Palmitate-loaded Nanostructured Lipid Carriers (VAP-NLC) were prepared by High Pressure Homogenization (HPH) technique. Trehalose, glucose, mannitol, α-lactin, D-sorbitol, D-maltose monohydrate and sucrose were chosen as the cryoprotectants. Also, the centrifugal stability of VAP-NLC dispersions after diluting different times and the effects of cryoprotectants on the diameter during the freeze-drying process were investigated extensively. Glucose and α-lactin proved to be the most effective in preventing particles aggregation during the VAP-NLC freeze-drying process. The most suitable concentrations were proved to be 5 wt% and 5 wt%, respectively when the dilution times of VAP-NLC dispersions reached 7 or more before freeze-drying. In conclusion, the NLC formulation demonstrated a promising perspective for oral delivery of poorly water-soluble actives in food industry.

scholarly journals ‘Preparation and characterization of a novel mucoadhesive carvedilol nano-sponge: a promising platform for buccal antihypertensive delivery’

2021 ◽  
Author(s):  
Nahed Mohamed Sallam ◽  
Rania Abdel-Basset Sanad ◽  
Mahgoub Mohamed Ahmed ◽  
EL Sayed Abdu Khafagy ◽  
Mamdouh Mostafa Ghorab ◽  
...  
Keyword(s):  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Heart Tissue ◽  
Buccal Delivery ◽  
Oxidative Stress Biomarkers ◽  
Tissue Quality ◽  
Sonication Technique ◽  
Pass Metabolism

Abstract Purpose: Carvedilol (CRV) is a non-selective beta-blocker used for hypertension treatment, angina pectoris, and heart failure. Oral administration of CRV showed poor bioavailability (25%), which may be due to exposure to the first-pass metabolism. Buccal delivery was used to boost its bioavailability.Methods: In this study carboxymethylcellulose/hydroxypropyl cellulose (CMC/HPC) composite buccal sponge enriched with CRV bilosomes was developed. Bilosomes were prepared using the thin-film hydration-sonication technique by applying a 32 -factorial design.Results: BL9 possessed the highest desirability value (0.861) and therefore, it was chosen as an optimal bilosomes. It exhibited a spherical shape with 217.2 nm, 87.13% entrapment efficiency, and a sustained release of CRV up to 24h. Consecutively, BL9 was incorporated in a CMC/HPC gel and lyophilized for 24 h to obtain a CMC-HPCL9 bilosomal sponge to enhance CRV buccal delivery. Morphological analysis of the prepared sponge with improved swelling showed a porosity of 67.58 percent. The in vivo assessment of rats indicates that the CMC-HPC/BL9 sponge enhances systolic/diastolic blood pressure, lipid profiles, oxidative stress biomarkers, and heart biomarkers with improved heart tissue quality.Conclusion: These results strongly encourage the use of this novel CMC-HPC/BL9 composite buccal sponge for the management of hypertension.

scholarly journals NANOSTRUCTURED LIPID CARRIERS (NLCS): A PROMINENT TOPICAL DELIVERY SYSTEM FOR COENZYME Q10 AND MYRICA ESCULENTA LEAVES EXTRACT FOR ANTI-AGING POTENTIAL

2018 ◽  
Vol 10 (8) ◽  
pp. 106 ◽  
Author(s):  
Prashant Kumar ◽  
Anita Singh
Keyword(s):  
Coenzyme Q10 ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carriers ◽  
Poloxamer 407 ◽  
Injection Technique ◽  
23 Factorial Design ◽  
Myrica Esculenta ◽  
Formulation Variables

Objective: In this study, Myrica esculenta (ME) leaves extract along with Coenzyme Q10 was entrapped in nanostructured lipid carriers (NLCs). The formulation variables were successfully optimized using 23 factorial design.Methods: Preparation of NLC was carried out by solvent injection technique. The formulation of NLC was consisting of the methanolic extract of Myrica esculenta leaves and Coenzyme Q10 as key ingredients. Oleic Acid (OA) was taken as liquid lipid, Precirol ATO 5 (PATO) as solid lipid, and Poloxamer 407 (P407). The formulation variables were statically optimized using 23 factorial design, followed by the characterization of the formulation.Results: The results demonstrated that the best optimized NLC formulation F5 of Coenzyme Q10 and plant extract exhibited the smallest size 169.46 nm and highest entrapment efficiency 90.06±0.171 %. Furthermore, it showed good physical stability at 28 d after preparation.Conclusion: The developed NLCs of Q10-ME extract from this study could be explored as the effective anti-ageing formulation.

scholarly journals PHYSICAL, CHEMICAL CHARACTERISTICS AND RELEASE RATE OF MELOXICAM FROM NANOSTRUCTURED LIPID CARRIERS (NLC) SYSTEM USING SOME CONCENTRATION RATIOS OF MONOSTEARIN AND ALPHA TOCOPHEROL ACETATE LIPID MATRIX

2017 ◽  
Vol 10 (2) ◽  
pp. 132
Author(s):  
Yulia Anggraeni ◽  
Isnaeni Isnaeni ◽  
Esti Hendradi
Keyword(s):  
Particle Size ◽  
Size Distribution ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carriers ◽  
Alpha Tocopherol ◽  
Chemical Characteristics ◽  
Lipid Matrix ◽  
Physical And Chemical Characteristics ◽  
Tocopherol Acetate ◽  
Physical And Chemical

Objective: The objective of this research is to develop nanostructured lipid carriers (NLC) of meloxicam (MLX) for topical application using monostearin and alpha-tocopherol as lipid matrix.Methods: MLX-NLC was prepared by high-shear homogenization, and it was characterized for organoleptic, pH, viscosity, particle size and size distribution, zeta potential, crystallinity, morphology, and entrapment efficiency. The six different ratios of solid and liquid lipid matrix were70:30, 75:25, 80:20, 85:15, 90:10, and 95:5, respectively.Results: The pH value of all formulas met the pH range of topical dosage at 4.5-6.5. Viscosity test showed that a trend toward the decrease in viscosity with the increase in alpha-tocopherol content was observed. The results of particle size test proved that all six NLC system formulas have <1000 nm particle size with a quite narrow particle size distribution. Scanning electron microscope revealed nearly spherical shape NLC with negligible effect ratios of solid and liquid lipid on the particle morphology. The X-ray diffraction result showed the decreasing of peaks intensity of MLX-NLC system. It showed that MLX in NLC system is amorphous. The result showed that the increasing concentration of liquid lipid in NLC system caused the rising entrapment efficiency of MLX.Conclusion: The result of this research indicates that the concentration ratio of monostearin and alpha-tocopherol acetate affects the physical and chemical characteristics of NLC system.Keywords: Nanostructured lipid carriers, Meloxicam, Monostearin, Physical and chemical characteristics, Entrapment efficiency. 

scholarly journals Stabilization of Deformable Nanovesicles Based on Insulin-Phospholipid Complex by Freeze-Drying

Pharmaceutics ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 539 ◽  
Author(s):  
Xu ◽  
Guo ◽  
Yang ◽  
Meng ◽  
Xia ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Phospholipid Complex ◽  
Long Term Stability ◽  
Protection Mechanisms ◽  
Drying Technology ◽  
High Entrapment Efficiency ◽  
Physical And Chemical

Deformable nanovesicles have been extensively investigated due to their excellent ability to penetrate biological barriers. However, suffering from serious physical and chemical instabilities, the wide use of deformable nanovesicles in medical applications is still limited. Moreover, far less work has been done to pursue the lyophilization of deformable nanovesicles. Here, we aimed to obtain stable deformable nanovesicles via freeze-drying technology and to uncover the underlying protection mechanisms. Firstly, the density of nanovesicles before freeze-drying, the effect of different kinds of cryoprotectants, and the types of different reconstituted solvents after lyophilization were investigated in detail to obtain stable deformable nanovesicles based on insulin-phospholipid complex (IPC-DNVs). To further investigate the underlying protection mechanisms, we performed a variety of analyses. We found that deformable nanovesicles at a low density containing 8% lactose and trehalose in a ratio of 1:4 (8%-L-T) have a spherical shape, smooth surface morphology in the lyophilized state, a whorl-like structure, high entrapment efficiency, and deformability after reconstitution. Importantly, the integrity of IPC, as well as the secondary structure of insulin, were well protected. Accelerated stability studies demonstrated that 8%-L-T remained highly stable during storage for 6 months at 25 °C. Based on in vivo results, lyophilized IPC-DNVs retained their bioactivity and had good efficacy. Given the convenience of preparation and long term stability, the use of combined cryoprotectants in a proper ratio to protect stable nanovesicles indicates strong potential for industrial production.

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