scholarly journals Optimization of Acid Protease Production byAspergillus nigerI1 on Shrimp Peptone Using Statistical Experimental Design

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Rayda Siala ◽  
Fakher Frikha ◽  
Samiha Mhamdi ◽  
Moncef Nasri ◽  
Alya Sellami Kamoun
Keyword(s):  
Culture Conditions ◽  
Medium Composition ◽  
Acid Protease ◽  
Protease Production ◽  
Statistical Experimental Design ◽  
Initial Ph ◽  
Burman Design ◽  
The Cost ◽  
Optimized Conditions ◽  
Plackett Burman Design

Medium composition and culture conditions for the acid protease production byAspergillus nigerI1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH2PO4, and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized conditions, the experimental protease production (183.13 U mL−1) closely matched the yield predicted by the statistical model (172.57 U mL−1) withR2=0.914. Compared with the initial M1 medium on which protease production was 43.13 U mL−1, a successful and significant improvement by 4.25 folds was achieved in the optimized medium containing (g/L): hulled grain of wheat (HGW) 5.0; KH2PO41.0; NaCl 0.3; MgSO4(7H2O) 0.5; CaCl2(7H2O) 0.4; ZnSO40.1; Na2HPO41.6; shrimp peptone (SP) 1.0. The pH was adjusted at 5 and the temperature at30°C. More interestingly, the optimization was accomplished using two cheap and local fermentation substrates, HGW and SP, which may result in a significant reduction in the cost of medium constituents.

Plackett-Burman Design for Screening Culture Conditions in Cellulase Production by Penicillium decumbens and Enzyme Characterization

2012 ◽  
Vol 518-523 ◽  
pp. 5578-5585
Author(s):  
Nan Ban ◽  
Yu Jie Zhou ◽  
Yan Ping Ye ◽  
Lin Mei Dai ◽  
Alatangaole Damirn ◽  
...  
Keyword(s):  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Penicillium Decumbens ◽  
Initial Ph ◽  
Effects Of Ph ◽  
Burman Design ◽  
The Stability ◽  
Plackett Burman Design

Plackett-Burman design was employed for screening culture conditions for cellulase production by Penicillium decumbens in submerged fermentation. The results showed that wheat bran was the most significant factor influencing Filter Paper Activity (FPA) of the cellulase, followed by cellulose microcrystalline and initial pH, which could be further optimized for improving the cellulase activity. The effects of pH and temperature on FPA assay were investigated, and optimal FPA could be obtained at pH 4.5 and 60 °C. The stabilities of endo-glucanase (EG), exo-glucanase (CBH) and β-glucosidase (BG) were investigated and compared with that of FPA under different pH and temperature. The results indicated that CBH and FPA were more sensitive to pH and temperature than EG and BG and the stability of CBH was very similar to that of FPA under the conditions.

Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin

2014 ◽  
Vol 522-524 ◽  
pp. 295-298
Author(s):  
Kai Wang ◽  
Pei Sheng Yan ◽  
Li Xin Cao
Keyword(s):  
Biological Control ◽  
Yeast Extract ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Peanut Hulls ◽  
Two Factors ◽  
Burman Design ◽  
Soya Peptone ◽  
Plackett Burman Design ◽  
Biocontrol Bacterium

Aflatoxins (AFs) are a series of highly toxic and carcinogenic secondary metabolites. In order to eliminate AFs contamination, biological control is one of the more promising techniques. In this study, we describe the optimization of media nutrients for the selected biocontrol bacterium, Lysinibacillus xylanilyticus strain BPM1. The strain was isolated from the peanut hulls in Shandong Province, China and exhibited antagonistic activity against aflatoxins. Maltose and sucrose were identified as best carbon source, while soya peptone and yeast extract as nitrogen source led to the highest OD600 observations. Medium composition was optimized using Plackett-Burman design, which was applied to find the key ingredients. The results revealed that the most significant two factors which were more effective in the fermentation of L. xylanilyticus BPM1 were soya peptone and yeast extract.

scholarly journals Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Li-mei Lin ◽  
Ling-jia Zhao ◽  
Jing Deng ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Steepest Ascent ◽  
Penthorum Chinense Pursh ◽  
Ft Ir ◽  
Experimental Yield ◽  
Burman Design ◽  
Anti Inflammatory ◽  
Penthorum Chinense ◽  
Optimized Conditions ◽  
Plackett Burman Design

Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

DESIGN OF CULTURAL CONDITIONS FOR PRODUCTION OF CHONDROITINASE BY ASPERGILLUS NIGER USING DESIGN OF EXPERIMENTS

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (06) ◽  
pp. 24-32
Author(s):  
N Kasinathan ◽  
◽  
V. M. Subrahmanyam ◽  
V. R Josyula
Keyword(s):  
Design Of Experiments ◽  
Yeast Extract ◽  
Spinal Cord Injuries ◽  
Box Behnken Design ◽  
Medium Components ◽  
Cultural Conditions ◽  
Burman Design ◽  
Level Of Significance ◽  
Optimized Conditions ◽  
Plackett Burman Design

Chondroitinases are useful in treating cancer and spinal cord injuries. The work reported so far does not focus on production of fungal chondroitinase. The present study focuses on the optimization of cultural conditions using design of experiments for production of chondroitinase by a fungal isolate CHCS-2, obtained from humus containing poultry waste. The cultural conditions were optimized systematically using a one-factor-at-a-time (OFAT) approach, factorial design, Plackett-Burman design (PBD) and Box- Behnken design to identify the composition of the production medium and the level of the variables. The level of significance of the medium components selected by OFAT study viz., yeast extract, chondroitin, and phosphate, was analyzed by factorial studies. The effect of eleven minerals on the enzyme yield was studied by PBD. The interaction among the medium components selected using factorial and PBD viz., yeast extract, cobalt and aluminum was investigated using the Box-Behnken design (BBD). Under the optimized conditions, maximum chondroitinase yield was 22.5 U/mL.

scholarly journals BNC Biosynthesis with Increased Productivity in a Newly Designed Surface Air-Flow Bioreactor

2020 ◽  
Vol 10 (11) ◽  
pp. 3850
Author(s):  
Izabela Cielecka ◽  
Małgorzata Ryngajłło ◽  
Stanisław Bielecki
Keyword(s):  
Mechanical Properties ◽  
Air Flow ◽  
Culture Conditions ◽  
Production Costs ◽  
High Yield ◽  
Flow Ratio ◽  
Culture Time ◽  
Wide Range ◽  
Burman Design ◽  
Plackett Burman Design

The application of bacterial cellulose (BNC) could be widely expanded if the production costs were reduced. This study aims to determine factors simultaneously affecting the yield and tensile strength of BNC in a newly designed surface air-flow bioreactor (SAF). For this purpose, a two-stage study was done. Firstly, the most important factors for high yield were determined based on the Plackett–Burman Design. Secondly, impact of the chosen variables on both responses was assessed in a wide range of factor values. The greatest influence on the yield and mechanical strength was proved for such factors as air-flow ratio, glucose concentration, and culture time. The productivity in a SAF bioreactor with controlled air-flow ratio was enhanced by 65%. In terms of mechanical properties, the stress of BNC membranes varied from 0.8 to 6.39 MPa depending on the culture conditions. The results of the performed tests make a useful basis for future optimizations.

scholarly journals Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp.

2020 ◽  
Vol 32 (6) ◽  
pp. 486-495 ◽  
Author(s):  
Tengku Athirrah Tengku-Mazuki ◽  
Kavilasni Subramaniam ◽  
Nur Nadhirah Zakaria ◽  
Peter Convey ◽  
Khalilah Abdul Khalil ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Phenol Degradation ◽  
Antarctic Bacterium ◽  
Burman Design ◽  
High Concentrations ◽  
Predicted Values ◽  
The Antarctic ◽  
Optimized Conditions ◽  
Plackett Burman Design ◽  
Central Composite

AbstractThis study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol.

scholarly journals Melamine Recognition: Molecularly Imprinted Polymer for Selective and Sensitive Determination of Melamine in Food Samples

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohadese Biabani ◽  
Azizollah Nezhadali ◽  
Ahmad Nakhaei ◽  
Hossein Nakhaei
Keyword(s):  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Food Samples ◽  
Quantitative Measurements ◽  
Sensitive Determination ◽  
Burman Design ◽  
Mip Sensor ◽  
Optimized Conditions ◽  
Plackett Burman Design

In this study, a sensitive and selective sensor is constructed to measure the melamine (MEL) using molecular imprinting polymer (MIP) technique. Chemical and electrochemical techniques are used to construct the MIP and quantitative measurements. The constructed sensor was modified with GO-Fe3O4@SiO2 nanocomposite. Screening and optimization of factors are done using statistical methods, including Plackett–Burman design (PBD) and central composite design (CCD). Under the optimized conditions, an MIP sensor showed a linear range from 5.0 × 10−7 to 1.0 × 10−5 M MEL concentration with a correlation coefficient (R2) of 0.9997. The limit of detection was obtained (0.028 µM) with a highly reproducible response (RSD 2.15%, n = 4). The electrochemical sensor showed good results for the determination of MEL in food samples.

Optimization of Culture Parameters for Expression of Recombinant Human Basic Fibroblast Growth Factor in Pichia pastoris Using Response Surface Methodology Combining with Plackett-Burman Design

2013 ◽  
Vol 647 ◽  
pp. 456-461 ◽  
Author(s):  
Rui Jiang ◽  
Li Wei Sun ◽  
Yu Zhao ◽  
Kai Feng ◽  
Shou Yan Chen
Keyword(s):  
Response Surface Methodology ◽  
Growth Factor ◽  
Pichia Pastoris ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Fermentation Time ◽  
Initial Ph ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Significant Factors

In this study, we used plackett-burman design (PBD) and response surface methodology (RSM) methods to optimize media and culture conditions for expression of recombinant human basic fibroblast growth factor (rhbFGF) in Pichia pastoris in order to effectively enhance the production of rhbFGF. PBD was used to screen the most significant factors from nine important factors, which were fermentation time, methanol, temperature, initial pH, OD600 for inducement, concentrated volume, YNB peptone, and casein. Then RSM was further used to optimize the significant parameters. Our results had shown that three significant factors selected by PBD were temperature, fermentation time, and methanol. Base on the 3-factor-3-level central composite design (CCD) of RSM, the final optimized conditions were fermentation time 126.7h, methanol 1.3%, temperature 21.3°C, initial pH 6.0±0.1, OD600 for inducement 26, concentrated volume 100 ml, YNB 13.4%, peptone 4%, and casein 0.1%. The maximum production of rhbFGF was 302.4mg/L, which was 2 times higher than the production of rhbFGF reported in the previous studies. Those results provided the foundation for further researches on higher density cultivation condition of rhbFGF explored in the fermenter and accelerate the industrialization development of rhbFGF.

scholarly journals Optimization of Large-Scale Culture Conditions for the Production of Cordycepin withCordyceps militarisby Liquid Static Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Chao Kang ◽  
Ting-Chi Wen ◽  
Ji-Chuan Kang ◽  
Ze-Bing Meng ◽  
Guang-Rong Li ◽  
...  
Keyword(s):  
Large Scale ◽  
Total Content ◽  
Culture Conditions ◽  
Key Factors ◽  
Working Volume ◽  
Large Scale Culture ◽  
Factor Design ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Cordycepin is one of the most important bioactive compounds produced by species ofCordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

scholarly journals Improvement of D-Ribose Production from Corn Starch Hydrolysate by a Transketolase-Deficient StrainBacillus subtilisUJS0717

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Zhuan Wei ◽  
Jue Zhou ◽  
WenJing Sun ◽  
FengJie Cui ◽  
QinHua Xu ◽  
...  
Keyword(s):  
Corn Starch ◽  
Corn Steep Liquor ◽  
Culture Conditions ◽  
Medium Composition ◽  
Production Performance ◽  
Raw Material ◽  
Initial Ph ◽  
Fermentation Parameters ◽  
Inoculum Volume ◽  
Starch Hydrolysate

D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability. The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnken factorial design. The optimal fermentation parameters were obtained as 36°C culture temperature, 10% inoculum volume, and 7.0 initial pH. The mathematical model was then developed to show the effect of each medium composition and their interactions on the production of D-ribose and estimated that the optimized D-ribose production performance with the concentration of 62.13 g/L, yield of 0.40 g/g, and volumetric productivity of 0.86 g/L·h could be obtained when the medium compositions were set as 157 g/L glucose, 21 g/L corn steep liquor, 3.2 g/L (NH4)2SO4, 1 g/L yeast extract, 0.05 g/L MnSO4·H2O, and 20 g/L CaCO3. These findings indicated the D-ribose production performance was significantly improved compared to that under original conditions.

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