The Preparation of Carbon Microspheres Loading Silver Antimicrobial Agent and the Study of its Properties

2012 ◽  
Vol 550-553 ◽  
pp. 1026-1029
Author(s):  
Jian Xi Ren ◽  
Jing Ya Li ◽  
Zhi Feng Cai ◽  
Jin Ming Dai ◽  
Mei Niu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Electron Microscope ◽  
Antibacterial Agent ◽  
Chemical Adsorption ◽  
Carbon Microspheres ◽  
Bacterial Inhibition ◽  
Good Antibacterial Activity ◽  
Scanning Electron

Carbon microspheres (CMSs) were used as the carrier to prepare the Ag-loading CMSs (Ag/CMSs) antibacterial agent through the method of chemical adsorption. The morphologies and structures of modified CMSs were characterized by using the field emission Scanning Electron Microscope (SEM). The results showed that silver was absorbed on the surface of CMSs. The bacterial inhibition ring experiment showed that Ag/CMSs had good antibacterial activity against Staphylococcus aureus and Escherichia coli, meanwhile the diameters of the bacterial inhibition rings were 19 mm against Staphylococcus aureus and 21 mm against Escherichia coli, respectively.

Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

2020 ◽  
Vol 15 (6) ◽  
pp. 665-679
Author(s):  
Alok K. Srivastava ◽  
Lokesh K. Pandey
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

Preparation, Characterisation, Crystal Structure and Antibacterial Activity of two Bis-Schiff Bases Containing a Piperazine Ring

2018 ◽  
Vol 42 (10) ◽  
pp. 512-514
Author(s):  
Rui-bo Xu ◽  
Xiao-tian Yang ◽  
Hai-nan Li ◽  
Peng-cheng Zhao ◽  
Jiao-jiao Li ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Schiff Bases ◽  
Piperazine Ring ◽  
X Ray ◽  
X Ray Crystallography ◽  
Good Antibacterial Activity

Two new bis-Schiff bases containing a piperazine ring, N,N‘-bis(4-chlorobenzylidene)- and N,N‘-bis(4-cyanobenzylidene)-1,4-bis(3-aminopropyl)piperazine, were prepared by the reaction of N,N‘-bis(3-aminopropyl)piperazine with 4-chloro- and 4-cyanobenzaldehyde, respectively. The dichloro compound was fully identified by X-ray crystallography and it exhibited good antibacterial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis.

Preparation and Antibacterial Effect of Nanopalygorskite Supported Nano-Silver Powder

2014 ◽  
Vol 1052 ◽  
pp. 327-331 ◽  
Author(s):  
Jian Rong Wang ◽  
Ya Li Liu ◽  
Bin Liu ◽  
Yan Ping Wu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Room Temperature ◽  
Antibacterial Effect ◽  
Silver Powder ◽  
Nano Silver ◽  
Good Antibacterial Activity ◽  
Novel Method ◽  
And Staphylococcus Aureus

A novel method was used to make palygorskite supported nano-silver powder at room temperature. XRD result showed the presence of silver in the final product. TEM investigation revealed that nano-sliver particles of 6-10 nm in diameter were successfully attached on surface of palygorskite fiber. FTIR was also used to clarify the formation mechanism of our method. The antibacterial effect of palygorskite supported nano-silver powder was investigated. Palygorskite supported nano-silver powder had good antibacterial activity on standard Escherichia coli and Staphylococcus aureus.

scholarly journals Hydrothermal Fabrication of Spindle-Shaped ZnO/Palygorskite Nanocomposites Using Nonionic Surfactant for Enhancement of Antibacterial Activity

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1453 ◽  
Author(s):  
Aiping Hui ◽  
Shuqing Dong ◽  
Yuru Kang ◽  
Yanmin Zhou ◽  
Aiqin Wang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Ionic Surfactants ◽  
E Coli ◽  
Antibacterial Performance ◽  
Good Antibacterial Activity ◽  
Controllable Growth ◽  
And Staphylococcus Aureus

In order to improve the antibacterial performance of natural palygorskite, spindle-like ZnO/palygorskite (ZnO/PAL) nanocomposites with controllable growth of ZnO on the surface of PAL were prepared in the presence of non-ionic surfactants using an easy-to-operate hydrothermal method. The obtained ZnO/PAL nanocomposites have a novel and special spindle-shaped structure and good antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and are also low cost. The minimum inhibitory concentrations of ZnO/PAL nanocomposites toward E. coli and S. aureus reached 1.5 and 5 mg/mL, respectively.

scholarly journals UJI AKTIVITAS ANTIBAKTERI ALGA HIJAU (Ulva sp.) DARI PANTAI SORIDO BIAK TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Melisa Selly Liswandari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Green Algae ◽  
Antibacterial Agent ◽  
Solvent Polarity ◽  
Negative Control ◽  
Diffusion Method ◽  
Significant Difference

UJI AKTIVITAS ANTIBAKTERI ALGA HIJAU (Ulva sp.) DARI PANTAI SORIDO BIAK TERHADAP BAKTERI Escherichia coli  DAN Staphylococcus aureus Melisa Selly Liswandari1), Daniel Lantang2), Septriyanto Dirgantara1)1)Program Studi Farmasi, Fakultas MIPA Universitas Cenderawasih, Jayapura2)Jurusan Biologi, Fakultas MIPA Universitas Cenderawasih, Jayapura, Kampus UNCEN Waena. Jl Perumnas III Waena Jayapura 99351 PapuaEmail*: [email protected]  ABSTRACT The green algae (Ulva sp.) are potentially as antibacterial agent because it contains triterpenoids, flavonoids, and saponins. The objective of this research are to know antibacterial activity of green algae (Ulva sp.) extracts using different levels of solvent polarity and the most effective concentration inhibits the growth of bacterial Escherichia coli and Staphylococcus aureus. Green algae (Ulva sp.) extracted by stratified soxletation method using solvent, eq diethyl ether, ethyl acetate, and ethanol 96%. Test antibacterial activity using the disc diffusion method. This research using the of CRD (Completely Randomize Design) with 7 treatments was concentration of 100 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm, positive control (ciprofloxacin 5µg) and negative control (aquades steril) with 3 repetitions. The data was analyzed using ANOVA and continued by HSD (Honestly Significant Difference) test with 95% confidence level. The result was demonstrated that extract green algae (Ulva sp.) the most effective to inhibits the growth of bacterial Escherichia coli is ethyl acetate extract with concentration 250 ppm (9,26 mm) and bacterial Staphylococcus aureus is ethanol 96% extract with concentration 750 ppm (9,57 mm). Keywords : Ulva sp., antibacterial agent, Escherichia coli, Staphylococcus aureus. ABSTRAK             Alga hijau (Ulva sp.) berpotensi sebagai antibakteri karena mengandung triterpenoid, flavonoid, dan saponin. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak alga hijau (Ulva sp.) yang menggunakan tingkat kepolaran pelarut berbeda dan konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Alga hijau (Ulva sp.) diekstraksi dengan metode sokletasi bertingkat menggunakan pelarut dietil eter, etil asetat, dan etanol 96%. Uji aktivitas antibakteri menggunakan metode difusi cakram. Penelitian ini menggunakan RAL dengan 7 perlakuan yaitu konsentrasi 100 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm, kontrol positif (ciprofloxasin 5µg) dan kontrol negatif (akuades steril) dengan 3 pengulangan. Data analisis menggunakan ANOVA dan dilakukan uji lanjut BNJ dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak alga hijau (Ulva sp.) yang paling efektif untuk menghambat pertumbuhan bakteri Escherichia coli adalah ekstrak etil asetat dengan konsentrasi 250 ppm (9,26 mm) dan bakteri Staphylococcus aureus adalah ekstrak etanol 96% dengan konsentrasi 750 ppm (9,57 mm). Kata kunci : Ulva sp., antibakteri, Escherichia coli, Staphylococcus aureus. 

scholarly journals Evaluation of anti bacteria effects of citrate-reduced silver nanoparticles in Staphylococcus aureus and Escherichia coli

2019 ◽  
Vol 10 (4) ◽  
pp. 3636-3643
Author(s):  
Hor Jia Wei ◽  
Mohd. Syafiq Awang ◽  
Nor Dyana ◽  
Daruliza Kernain ◽  
Yazmin Bustami
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Reduction Method ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Vis Spectroscopy ◽  
Mic Value

Silver nanoparticles (AgNPs) has long known for its inhibitory and bactericidal effects. However, due to its’ attractive antibacterial property, on-going research with various synthesis strategies actively been conducted. In this study, the synthesis of AgNPs was reported, using a simple chemical reduction method with citrate as the reducing agent TEM was used to characterize the obtained AgNPs. Then, Staphylococcus aureus and Escherichia coli were used to identify the antibacterial activity of AgNPs. The inhibition effects of AgNPs against these two bacteria were observed via disc diffusion, and MIC assays and the effects of AgNPs mode of action on both bacteria were further observed under TEM. The formation of AgNPs at ̴ 400 nm, which is the surface plasmon resonance peak was observed using Uv-Vis spectroscopy. The size of AgNPs mostly in the range of 1-10 nm and their morphology appeared to be spherical. Based on the MIC assay, Escherichia coli exhibit low MIC value with 0.049 mg/ml as compared to Staphylococcus aureus with0.391 mg/ml MIC value; correspond to the effective antibacterial activity by the citrate-reduced AgNPs. Further observation on the bacterial surface structure can be seen with cross-sectional TEM image, and it provides an insight into the AgNPs mechanistic aspects of AgNPs against Staphylococcus aureus and Escherichia coli. Silver nanoparticles have been successfully synthesised using the citrate reduction method. Results obtained in this study thus elucidating promising findings to employed AgNPs as an antibacterial agent, and this composition needs to be further study and develop into an antibacterial agent.

Synthesis of New Piperazine Substituted Chalcone Sulphonamides as Antibacterial Agents

2020 ◽  
Vol 17 (2) ◽  
pp. 136-143
Author(s):  
Yan-Ling Tang ◽  
Yong-Kun Li ◽  
Min-Xin Li ◽  
Hui Gao ◽  
Xiao-Bi Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Infections ◽  
Antibacterial Agents ◽  
Good Antibacterial Activity ◽  
In Vitro Antibacterial Activity ◽  
Global Threat

Background: Infection is a global threat to human health, and there is an urgent need to develop new effective antibacterial drugs to treat bacterial infections. Objective: To study the antibacterial activity of piperazine substituted chalcone sulphonamides. Materials and Methods: A series of novel piperazine substituted chalcone sulphonamides have been prepared, and in vitro antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli strains were evaluated. Results: The results showed that derivatives 6a, 6c and 6h displayed good antibacterial activity against Bacillus subtilis with MIC values of 4.0-8.0 mg/mL. Conclusion: Piperazine substituted chalcone sulphonamides may be used as potential antibacterial agents.

scholarly journals SINTESIS DAN KARAKTERISASI NANOPARTIKEL CuFe2O4 SERTA APLIKASINYA SEBAGAI ANTIBAKTERI

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Almendo G. Lasera ◽  
Henry Aritonang ◽  
Harry Koleangan
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Gram Negative Bacteria ◽  
E Coli ◽  
Activity Test ◽  
Sem Imaging ◽  
Scanning Electron

ABSTRAKNanopartikel CuFe2O4 disintesis menggunakan metode kopresipitasi. Material yang telah disintesis, kemudian dikarakterisasi dengan menggunakan Scanning Electron Microscopy (SEM) dan Xray-Diffraction (XRD) serta di uji aktifitasnya sebagai antibakteri dengan menggunakan bakteri Staphylococcus aureus (Gram positif) dan Escherichia coli (Gram negatif). Hasil citra SEM memperlihatkan morfologi nanopartikel CuFe2O4 berbentuk persegi yang tidak beraturan. Difraktogram XRD menunjukkan bahwa ukuran sampel CuFe2O4 yang diperoleh adalah sebesar 20,136 nm. Uji aktifitas antibakteri yang telah dilakukan menunjukkan bahwa, nanopartikel CuFe2O4  jauh lebih baik dalam menghambat pertumbuhan bakteri E. coli dibandingkan dengan bakteri S.aureus, masing-masing 24 mm dan 6,5 mm. ABSTRACTCuFe2O4 nanoparticles were synthesized using the coprecipitation method. The synthesized material was then characterized using Scanning Electron Microscopy (SEM) and Xray-Difraction (XRD) and tested as antibacterial activity using Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) bacteria. SEM imaging showed morphology of CuFe2O4 nanoparticles shaped like a rod. XRD diffractogram showed that the size of CuFe2O4 nanoparticles that obtained at 20.13 nm. The antibacterial activity test that has been done shows, CuFe2O4 nanoparticles are much better in inhibiting the growth of E. coli bacteria compared with S. aureus bacteria, 24 mm and 6,5 respertivelly. 

scholarly journals SINTESIS KOMPOSIT KITOSAN/MgO/Ag DAN ANALISIS EFEKTIVITASNYA SEBAGAI ANTIBAKTERI

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shintya Maryani ◽  
Henry F Aritonang ◽  
Vanda S Kamu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Coprecipitation Method ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Ag Particles ◽  
Scanning Electron

Penelitian tentang sintesis komposit kitosan/MgO/Ag dengan variasi massa prekursor AgNO3 0,05 g, 0,1 g, 0,15 g dan 0,2 g disintesis dengan metode kopresipitasi. Komposit yang dihasilkan dikarakterisasi menggunakan X-Ray Diffractometry (XRD) dan Scanning Electron Microscope (SEM). Selanjutnya, keefektifan komposit ini sebagai antibakteri diuji yaitu Staphylococcus aureus (Gram positif) dan Escherichia coli (Gram negatif). Hasil penelitian menunjukkan bahwa puncak karakteristik partikel MgO dan Ag tidak muncul pada difraktogram XRD, tetapi secara visual dan morfologis SEM mendukung pembentukan dua partikel dan berdasarkan literatur dari hasil peneliti lain. Selain itu, komposit ini efektif dalam menghambat pertumbuhan kedua bakteri.       ABSTRACT Research on the synthesis of chitosan/MgO/Ag composites with variations in the mass of AgNO3 precursors 0.05 g, 0.1 g, 0.15 g and 0.2 g were synthesized by the coprecipitation method. The resulting composites were characterized using X-Ray Diffractometry (XRD) and Scanning Electron Microscope (SEM). Furthermore, the effectiveness of this composite as an antibacterial was tested, namely Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). The results showed that the characteristic peaks of MgO and Ag particles did not appear on the XRD diffractogram, but visually and morphologically SEM supported the formation of two particles and based on the literature from the results of other researchers. In addition, this composite is effective in inhibiting the growth of both bacteria.

scholarly journals Synthesis and Antibacterial Testing of Imina Derivative Compounds from Piperonal and Anilin

2018 ◽  
Vol 21 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Dimas Syamsudin ◽  
Ismiyarto Ismiyarto ◽  
Ngadiwiyana Ngadiwiyana
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Melting Point ◽  
1H Nmr ◽  
Antibacterial Agent ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
Inhibition Zone

Synthesis of Imine derivates used as antibacterial activity has been done. Synthesis was conducted by condensation reaction between piperonal and aniline. The product synthesis was characterized by melting point, LC-MS, FTIR and 1H-NMR, then application of the product as antibacterial towards bacteria Staphylococcus aureus and Escherichia coli. The product of synthesis was pale-brown solid, and had melting point at 56-61 0C. Identification by LC-MS showed the compound molecular weight was 225.30 gram/mol, identification by FTIR showed a compound contained imine C=N at 1620.21 cm-1, C-H aromatic at 1581.63 cm-1, Csp3-H at 2962.37 cm-1 from CH2, and C-O at 1265.30 cm-1, Identification by 1H-NMR showed a compound had a singlet peak at δ=6 ppm that proton was from the CH2, multiplet peak at δ=6.9-7.7 ppm proton from two aromatics and singlet peak at δ=8.4 ppm from proton imine. The minimum inhibitory concentration of the product towards Staphylococcus aureus and Escherichia coli was 1 mg/mL with diameter inhibition zone 2 mm and 3 mm. From these data it could be concluded that the compound 3,4- methlenedioxybenzilyimine was formed and it had ability as an antibacterial agent.

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