Research for Biodegradation and Bioaugmentation of Drilling Wastewater

2013 ◽  
Vol 684 ◽  
pp. 226-229
Author(s):  
Feng Xu ◽  
Jin Chuan Gu ◽  
Jin Bao

The paper studied the degradation rate of single strain, the microorganisms agent compatibility and the bioaugmentation effects of the activated sludge system. The results showed that 10 strains screened from drilling site were able to degrade drilling wastewater. Confirm the optimal ratio of strains’ compatibility through orthogonal experiment. In augmented experiments, it showed that in the control SBR system with the COD of the effluent was in three different average levels (with two load shock), the COD degradation rates were 21.4%, 22.8% and 6.9%. In the augmented system treated with the same effluent, the degradation rates of augmented system were 64.8%, 62.78% and 66.9% respectively. It showed that the activated sludge was augmented by the mixed cultures which made the system to be more stable and improve impact resistance.

2011 ◽  
Vol 183-185 ◽  
pp. 301-305
Author(s):  
Feng Xu

The biodegradation of mixed cultures was analyzed. The paper studied the effects of treat condition (static and shaking), concentration of dye, activities of oxidative enzymes (LiP, laccase and Manganese Peroxidase) and the bioaugmentation effects of the activated sludge system. The results showed that mixed cultures were able to degrade Reactive Brilliant Red X-3B and Direct Blue-6 (100 mg/L) completely within 32 h in shaking condition. As the initial concentration of Reactive Brilliant Red X-3B increased from 100mg/L to 900mg/L, the decolorization rate still maintain a high level only decreased from 99.3% to 83.5%. Induction in the activity of oxidative enzymes (LiP, laccase and Manganese Peroxidase) represented their role in degradation. In augmented experiments, it showed that in the control SBR system with the CODCr of the effluent was in three average levels 848, 1228, and 1550 mg/L, the CODCr degradation rates were 42.3%, 32.8% and 19.7%. In the augmented system treated with the same effluent, the degradation rates of augmented system were 86.5%, 71.8% and 59.8% respectively. It showed that the activated sludge was augmented by the mixed cultures which made the system to be more stable and improve impact resistance.


2020 ◽  
Vol 35 (3) ◽  
pp. 457-463
Author(s):  
Huixia Lan ◽  
Xiangzhi Wang ◽  
Shixin Qi ◽  
Da Yang ◽  
Hao Zhang

AbstractUsing the acclimated activated sludge from the pulping middle-stage effluent, the effect of pH shock on the micro-oxygen activated sludge system with a nano-magnetic powder/graphene oxide composite was studied. The results showed that the removal rates of chemical oxygen demand (CODCr) and ultraviolet adsorption at 254 nm (UV254) decreased. Also, the sludge settling performance was poor due to the impact of pH, but the impact resistance of nano-magnetic powder/graphene oxide group (MGO group) was higher and the recovery was faster. Results of high throughput sequencing indicated that the diversity of microbial community was reduced by the impact of pH, but it was significantly higher in MGO group than in the blank group. The dominant bacteria after pH shock or recovery in both of the system had a large difference. The percentage of the dominant bacteria in the MGO group was higher than that in the blank group. The MGO group had higher electron transfer system (ETS) activity which made the system having a strong pH impact resistance.


2011 ◽  
Vol 179-180 ◽  
pp. 781-786 ◽  
Author(s):  
Jian Yang Feng ◽  
Xiao De Zhou

Six strains named SY1 to SY6 were isolated from oil-polluted soil. The degradation tests of oil by our isolates were carried out under still-reaction-bottle-aeration and shaking-aeration conditions. The co-metabolic characteristics of two co-substrates (ethanol and glucose) and inorganic ions on the biodegradation of oil by our isolates were also studied. The results show that oil can be degraded by the six isolates effectively. The degradation ability of strains is enhanced due to co-substrates added. The enhanced roles of glucose are stronger than ethanol to SY1 and SY5; while the enhanced roles of ethanol are stronger than glucose to SY3.And the enhanced roles of glucose and ethanol are similar to SY2, SY4 and SY6. Inorganic ions, such as Ca2 + Fe2 +, Mg2+, etc, could promote the degradation ability of strains and improve the growth of strains. The degradation effects of mixed strains were inferior to that of single strain. After five days, the degradation rate of SY3 was worst, which was 65.7%. In the mixed strains, there were only 11 groups’ degradation rates greater than that of SY3, and other 46 groups’ degradation rates were less than that of SY3.


2012 ◽  
Vol 217-219 ◽  
pp. 918-922 ◽  
Author(s):  
Jian Tao Niu ◽  
Xiao Ying Zhang ◽  
Na Na Pei ◽  
Qi Lei Tian

Fabrics of cellulose fibers such as cotton, ramie and viscose were buried in activated sludge, the biodegradability of cellulose fibers and their fabrics were studied. And the relations between degradation rates, morphological structure and crystal structure of these fibers and degradation time were analyzed. The results indicated that, under the same conditions, degradation rate of viscose fiber was higher than that of cotton and ramie fibers. With the increase of degradation time, breakage to the fabrics occurred, meanwhile, fibers showed an increase of surface roughness and displayed a lot of tiny cavities and cracks. In the process of degradation, these cellulose fibers of cotton, ramie and viscose all formed new diffraction peaks near the place of 2θ=26.5°, in addition, the degrees of crystallinity of these fibers were fluctuated.


2017 ◽  
Vol 77 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Xiangyu Guan ◽  
Fei Liu ◽  
Jing Wang ◽  
Caoxiang Li ◽  
Xiaoxuan Zheng

Abstract 1,4-Dioxane (dioxane), a probable human carcinogen, often exists in industrial wastewater and domestic sewage. In this study, we applied 16S rRNA and metatranscriptomic methods to analyze the dioxane biodegradation mechanism by activated sludge. Tetrahydrofuran (THF) was added as an essential co-metabolite to promote the degradation of dioxane. We found the dioxane removal ratio increased with increasing THF concentrations. When the THF concentration increased from 60.0 to 200.0 mg/L, the dioxane degradation rate was stable. Three additions of ∼60.0 mg/L THF resulted in better dioxane degradation than one addition of 200 mg/L THF. Ammonia-oxidizing and denitrifying bacteria with methane monooxygenases (MOs) and ammonia MOs played the most important roles during the degradation of dioxane. Kyoto Encyclopedia of Genes and Genomes metabolic pathway and functional genes analyses showed that the activated sludge system was complex and stable when dioxane was added. In future studies, primers should be designed to identify specific bacteria and functional MO genes, which would help reveal the function of various bacteria and their MOs during dioxane degradation.


2018 ◽  
Author(s):  
Gede H Cahyana

As a part of activated sludge system, oxidation ditch has a special form like a canal without end point. Rotor is used to diffuse oxygen from air to the water.


1974 ◽  
Vol 9 (1) ◽  
pp. 235-249 ◽  
Author(s):  
S.G. Nutt ◽  
K.L. Murphy

Abstract Conventional wastewater parameters are accepted as inadequate estimates of the condition of activated sludge but numerous other indices have been suggested as specific measurements of the activity and viability of the biomass. Literature in the related fields of microbiology and biochemistry were reviewed in order to select the most appropriate activity parameters for application to a heterogeneous biological material. Modified analytical methods were applied to a well-controlled biological system containing a single predominant bacterial species to evaluate the relative merit of each as an indicator of viability and activity. The potential of each parameter in a complex heterogeneous system was determined by monitoring each index in a bench activated sludge system. The predominant culture studies indicated that the ATP content of the biomass and the dehydrogenase activity were potential indicators of cell viability in a simple system. However, in the complex activated sludge system, only the ATP content showed significant correlation to the organic carbon removal rate.


1994 ◽  
Vol 30 (3) ◽  
pp. 73-78 ◽  
Author(s):  
O. Tünay ◽  
S. Erden ◽  
D. Orhon ◽  
I. Kabdasli

This study evaluates the characterization and treatability of 2,4-D production wastewaters. Wastewaters contain 20000-40000 mg/l COD, 17000-30000 mg/l chloride and pH is around 1.0. Chemical oxidation with hydrogen peroxide provided almost complete COD removal. The optimum conditions are 3:1 H2O2/COD oxidant dosage, 3000 mg/l Fe3+ as catalyst and pH 3. Partial oxidation at 0.5:1 H2O2//COD ratio is also effective providing 67% COD removal. A batch activated sludge system is used for biological treatability. Dilution is needed to maintain a tolerable chloride concentration which increases through COD removal. pH also increased during COD removal. 85% COD removal is obtained for the 50% dilution at an organic loading of 0.3 day‒1 on a COD basis. Completely and partially oxidized wastewaters are also treated in the activated sludge down to 30 mg/l BOD5.


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