Study on Extraction Process and Content Determination of Safflor Yellow

2013 ◽  
Vol 781-784 ◽  
pp. 663-667 ◽  
Author(s):  
Jiang Li Nie ◽  
Cong Shi ◽  
Guo Yan Yan ◽  
Yi Pei
Keyword(s):  
Extraction Process ◽  
Scientific Basis ◽  
Orthogonal Test ◽  
Extraction Rate ◽  
Single Factor ◽  
Content Determination ◽  
Uv Visible ◽  
Herbal Quality ◽  
Optimum Extraction

The aim was to study the extraction process of safflor yellow (SY) from Cacthamus tinctorius. The SY contents of five varieties which were planted in safflower production base in Tianjin were measured and compared, in order to determine its growth adaptability and herbal quality. [Method Based on the single-factor test, the optimum extraction process can be obtained after selecting the lager factor which affected extraction rate of safflor yellow. Then the SY contents of five varieties were determined by UV-visible spectrophotometer. [Result The orthogonal test showed that the optimal extraction process was to add 10 and 12 times amount of water and extract for 30min every time at 60°C, totally for 2 times. The SY contents of five varieties were significantly higher than or equal to the standard medicinal safflor. [Conclusion Five varieties of safflower which were planted in Tianjin showed good adaptability and herbal quality. This study would provide scientific basis for improving saline-alkali soil in Tianjin.

Optimization of Extraction Conditions of Essential Oil from Latifolia miq Roots and Stems with Supercritical CO2 Fluid

2013 ◽  
Vol 726-731 ◽  
pp. 4512-4516
Author(s):  
Ren Yong Gu ◽  
Zhi Ping Li
Keyword(s):  
Essential Oil ◽  
Optimum Condition ◽  
Raw Materials ◽  
Extraction Process ◽  
Orthogonal Test ◽  
Single Factor ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

Studying on the extraction process of essential oil from Latifolia Miq roots and stems by Supercritical CO2 extraction. The effects on extracting ratio were investigated by using single factor test, adding content of grinding fineness of raw materials,extracting temperature, extracting pressure and extracting time. The optimum extraction condition was determined by orthogonal test. The results showed that the optimum condition of the extraction was as follows: grinding fineness 90 mesh, extracting temperature 45 °C, extracting pressure 20 MPa and extracting time 2.5 h.

scholarly journals A study on the preparation and quality standard of Saifula granules

2021 ◽  
pp. 1-12
Author(s):  
Maimaiti Aisha ◽  
Atikanmu Wahafu ◽  
Yilifanjiang Kuerban ◽  
Akenmujiang Aierken ◽  
Refuhati Saimaiti ◽  
...  
Keyword(s):  
Process Parameters ◽  
Quality Standard ◽  
Extraction Process ◽  
Scientific Basis ◽  
Orthogonal Test ◽  
Quality Control Standard ◽  
Material Basis ◽  
Good Repeatability ◽  
Total Extraction

Objective: To establish the decocting and formulation technology of Saifula granules, and establish a reliable TLC identification quality control standard. Methods: Orthogonal test was used to optimize the water extraction process and TLC was used for qualitative identification. Results: To optimize the process parameters of SFL granules, first, the content of total flavonoids and the total extraction rate were taken as the indexes. Through single factor and orthogonal test, the optimal extraction process was determined as follows: extraction time 1 h, solvent multiple 1:10, extract three times. After the process parameters were determined, in order to further explain the chemical composition of SFL extract, 15 compounds in SFL extract were identified by UHPLC-Q-Orbitrap-MS, 13 batches of SFL extracts were prepared, and their fingerprints were analyzed. The results showed that there were 28 common characteristic peaks in the 13 batches of SFL extracts. The similarity evaluation results of fingerprints showed that the similarities of 13 batches of SFL extracts were greater than 0.9. An HPLC-DAD method for the simultaneous determination of gallic acid, corilagin, and ellagic acid was established. The method has good repeatability, stability, and accurate results. Conclusion: The standard can comprehensively reflect the material basis of SFL granules, and the above methods are accurate, easy to operate, stable and feasible, and provide scientific basis and basis for the development, promotion, and clinical application of SFL granules.

scholarly journals Study on Extraction Technology of Pitaya Polysaccharide

2021 ◽  
Vol 251 ◽  
pp. 02052
Author(s):  
Feng Xuehua ◽  
Song Zurong ◽  
Tao Ali ◽  
Gong Panpan
Keyword(s):  
Orthogonal Test ◽  
Raw Material ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Single Factor ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Solid Liquid ◽  
Optimum Extraction

Using pitaya as raw material, this paper used ethanol subsiding method to study the extraction technology of pitaya polysaccharide by single factor test and orthogonal test. Through orthogonal test, we have found the optimum extraction conditions of pitaya polysaccharide as follows: extraction temperature 60℃, solid-liquid ratio 1:30, extraction time 3h. Under the optimal extraction conditions, the yield of pitaya polysaccharide was 1.13%.

scholarly journals Content Determination of Active Component in Huangqi Yinyanghuo Group and Its Effects on hTERT and Bcl-2 Protein in Osteosarcoma

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ying Tan ◽  
Lei Tan ◽  
Shuai Huang ◽  
Junfan Lu ◽  
Longtan Yu
Keyword(s):  
Cell Survival ◽  
Active Component ◽  
Extraction Process ◽  
Osteosarcoma Cell ◽  
Human Telomerase Reverse Transcriptase ◽  
Logarithmic Growth Phase ◽  
Survival Fraction ◽  
Duration Of Action ◽  
Content Determination

To screen the optimal extraction process and content determination of active component of Huangqi Yinyanghuo group (HYG) and to study the effects of HYG on human telomerase reverse transcriptase (hTERT) and Bcl-2 protein in osteosarcoma (HOS) cells, providing the theoretical basis for clinical application of HYG in treatment of osteosarcoma, orthogonal design table L9(43) was used to design the extraction process of HYG, and icariin was taken as the investigation index to optimize the extraction process of HYG. 0.125, 0.25, 0.5, 1, 2, 4, and 8 μmol/L HYG were taken to act separately on logarithmic growth phase osteosarcoma HOS cells, CCK-8 assay was used to determine cell viability, and immunohistochemical SP assay was used to determine the expression of hTERT and Bcl-2 protein. Apoptosis rate was positively correlated with the dose of HYG, and the expressions of hTERT and Bcl-2 protein were significantly decreased with the prolonged duration of action. Under the effect of HYG, dose was negatively correlated with osteosarcoma cell survival fraction; osteosarcoma cell survival fraction was positively correlated with hTERT and Bcl-2 protein; duration of action was negatively correlated with hTERT and Bcl-2 protein; and hTERT and Bcl-2 protein were in a synchronous relationship.

scholarly journals Study on the Effect of Different Extraction Methods in Four Pharmacopoeia on the Content and Structural Transformation of Ginsenoside and the Optimization of Extraction Process

2021 ◽  
Author(s):  
Kai Sun ◽  
Hua Jiang ◽  
Pingya Li ◽  
Lei Xu ◽  
Yaling Deng ◽  
...  
Keyword(s):  
United States ◽  
Structural Transformation ◽  
Extraction Method ◽  
The United States ◽  
Extraction Process ◽  
Extraction Methods ◽  
Detection Methods ◽  
Content Determination ◽  
Q Exactive

Abstract Background: In recent years, ginseng products are widely used in various fields. More and more people pay attention to the extraction methods and quality evaluation of ginseng. At present, China, the United States, Europe, Japan and Korea have the quality standards and content determination methods of ginseng. However, due to the different treatment methods adopted before the determination of ginseng samples, the content limits of the index components, such as ginsenoside Rb1, Rg1 and Re are also different. There have been literature analyzed the similarities and differences of ginseng content detection methods in pharmacopoeias of different countries, but the comparison of the effects of different methods on ginsenoside content and structural transformation has not been reported.Methods: In this paper, ginsenosides in ginseng were extracted according to four national Pharmacopoeia, and analyzed quantitatively and qualitatively by UPLC-Q-Exactive-MS and HPLC-UV. Finally, a simple and feasible extraction method was optimized by response surface method. Results: Twelve kinds of ginsenosides in ginseng were quantitatively analyzed by using the methods of four national pharmacopoeia. Among them, the contents of Rg1, Re and Rd were high, and they were the highest by using unheated J/KP (Japan/Korea Pharmacopoeia) method. Ten kinds of ginsenosides were determined by heated CP (China Pharmacopoeia), USP (the United States Pharmacopoeia) and EP (European Pharmacopoeia) method, and seven kinds of ginsenosides were determined by unheated J/KP method. In the following UPLC-Q-Exactive-MS study, 34, 36, 21 and 19 ginsenosides were identified by CP, USP, EP and J/KP method, respectively. In the optimization of ginsenoside extraction process, an efficient extraction method was selected from the solvent, extraction time, solid-liquid ratio and other factors. In conclusion, through the qualitative and quantitative comparison of CP, USP and EP samples after heating, it can be seen that ginsenoside heating will increase the content of rare saponins, and the heating time is directly proportional to the content of rare saponins. Conclusion: The pretreatment method has a significant effect on the content determination of ginseng. The analysis of the preparation method and process optimization of the four Pharmacopoeia can provide important reference for the revision of ginseng standard.

scholarly journals Optimization of Extraction Process of Methyl Eugenol and Asarinin in Asarum with Deep Eutectic Solvent Based on the Response Surface Methodology

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Huiwei Bao ◽  
Yang Xu ◽  
Yuejie Wang ◽  
Tao Zhang ◽  
Enpeng Wang ◽  
...  
Keyword(s):  
Response Surface ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Methyl Eugenol ◽  
Extraction Rate ◽  
Process Conditions ◽  
Surface Model ◽  
Single Factor ◽  
Extraction Technology ◽  
Ultrasonic Time

To explore a green and efficient extraction technology for the extraction of active ingredients of Asarum, the deep eutectic solvent combined with ultrasonic was applied to compare the extraction efficiency of 10 kinds of deep eutectic solvents, taking the extraction rate of methyl eugenol and asarinin as indices. Single-factor experiments were adopted to investigate the influence of molar ratio, liquid-to-solid ratio, eddy time, ultrasonic time, and temperature of the deep eutectic solvent on the extraction rate of methyl eugenol and asarinin. Based on single-factor experiments, the surface response methodology was used to optimize the extraction process conditions. The results showed that the optimum extracting process conditions of methyl eugenol and asarinin in Asarum consisted of a ratio of choline chloride to glycerol of 1 : 3, a DES volume of 2 mL, an ultrasonic temperature of 60°C, an ultrasonic time of 30 min, and a vortex oscillation of 7 min. Under the optimum extracting process conditions, the contents of methyl eugenol and asarinin were 1.9428 mg/g and 0.9989 mg/g, respectively, and the comprehensive index was 2.3280 (RSD of 1.91%). The results were close to the predicted values of the response surface model, demonstrating the applicability of the model. The extraction rate of methyl eugenol and asarinin in Asarum by this method was higher than that of water extraction and alcohol extraction, which fully indicated the high efficiency of ultrasonic-assisted green deep eutectic solvent extraction technology. The results provide data support for further development and utilization of Asarum.

Optimizing the Extraction Process of Rubusoside from the Rubus Suavissimus: A Cellulase Pretreatment Approach

2014 ◽  
Vol 707 ◽  
pp. 144-148 ◽  
Author(s):  
Xiao Hong Zhu ◽  
Ming Xia ◽  
Chun Xing Yang
Keyword(s):  
Optimal Condition ◽  
Extraction Efficiency ◽  
Ph Value ◽  
Extraction Process ◽  
Orthogonal Test ◽  
Single Factor ◽  
Enzyme Substrate ◽  
Substrate Ratio ◽  
A New Technique ◽  
Single Factor Experiment

The paper discussed the extraction process of rubusoside from Rubus suavissimus using a new technique named cellulase pretreatment approach. First, the parameters of cellulose which generate the optimized condition for the extraction of rubusoside from Rubus suavissimus were identified. Under the most optimal condition, the extraction efficiency is maximal. The parameters include pH value,temperature and enzyme-substrate ratio. Second, single factor experiment and an orthogonal test then could be conducted to obtain the optimized condition for the extraction process with the assistances of cellulose. The parameters under the optimized condition were determined as following: ethanol as solvent, 1.2% cellulose, 40°C, pH 4.8 and duration 25 minutes. The result shows that the extraction efficiency of rubusoside is 10.81%.

scholarly journals Orthogonal Optimization of Polysaccharide Extraction from Millet Bran

2019 ◽  
Vol 78 ◽  
pp. 02011
Author(s):  
Songling Cai ◽  
Min Pang
Keyword(s):  
Extraction Method ◽  
Extraction Process ◽  
Orthogonal Test ◽  
Extraction Rate ◽  
Extraction Time ◽  
Alkali Concentration ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Optimum Parameters ◽  
Orthogonal Optimization

This paper investigated the effects of liquid-solid ratio, extraction time, extraction temperature and alkali concentration on the extraction rate of millet bran polysaccharides by the enzymatic hydrolysis and alkaline extraction method, then the L9 (34) orthogonal test was designed to optimize the extraction process according to the single factor experiment.The optimum parameters were obtained as follow: 15:1 liquid-solid ratio, five hours extraction time, 80° extraction temperature, and 0.7 mol/l concentration of sodium hydroxide. The extraction rate of millet bran polysaccharide reached 11.46% through the verification experiments under these conditions,and the RSD was 1.79%. Therefore, it can provide some experimental basis for the scientific research of millet bran polysaccharides in the future.

Light Side of Design: Professional Vector

2020 ◽  
pp. 23-33
Author(s):  
Elena A. Zaeva-Burdonskaya ◽  
Yuri V. Nazarov
Keyword(s):  
Professional Practice ◽  
Wide Spectrum ◽  
Scientific Basis ◽  
Design Culture ◽  
Interdisciplinary Nature ◽  
Educational Programmes ◽  
Design Activities ◽  
The Subject ◽  
Light Side

This article addresses one of the most actively developing types of design activities – light design. The article comprises quotes of the leading Russian and foreign light design specialists published over the previous five years, as well as the authors’ own conclusions. The thoughts quoted in the article are sometimes opposite to each other and reflect the wide spectrum of professional practice. They reflect the initial opinions of analysts and experts which are often diverging. All of the specialists point at the interdisciplinary nature of the new profession, which imposes additional load on a designer overloaded enough already by the scope and speed of the problems being solved nowadays. The discussion of the new profession of light designer initiated on the pages of professional publications is especially important in view of the development of professional standards and standards of design and architectural education, as well as creation of new educational programmes based on various approaches to the subject in technical and humanitarian institutions. The goal of this article is to introduce light design into the field of fully legitimate sections of design culture, to define the authentic scientific basis of the new creative profession, to initiate a foundation for self-determination of the new synthetic area, which materially affects the state of the profession as a whole and the life standards of a wide variety of consumers. In order to reach the set goal, a comparative and analytical method of study was selected, which allows studying the problem to a large extent and from all angles and finding the ways of overcoming the challenges emerging in the area of the new activity.

Science of Ahangyol and Its Importance for Modern Scientific Thought

2019 ◽  
Vol 10 (5) ◽  
pp. 473-478
Author(s):  
Ahmad Gashamoglu ◽  
Keyword(s):  
Modern Science ◽  
Scientific Basis ◽  
New Paradigm ◽  
Islamic Philosophy ◽  
Scientific Thought ◽  
Religious Books ◽  
Strategic Approach ◽  
Scientific Potential ◽  
Made In

The Article briefly discusses the need for generation of the Science of Ahangyol, and this science’s scientific basis, object and subject, category system, scientific research methods and application options. Ahangyol is a universal science and may be useful in any sphere. It may assist in problem solving in peacemaking process and in many areas such as ecology, economics, politics, culture, management and etc. This science stipulates that any activity and any decision made in the life may only and solely be successful when they comply with harmony principles more, which are the principles of existence and activity of the world. A right strategic approach of the Eastern Philosophy and the Middle Age Islamic Philosophy and scientific thought has an important potential. This strategic approach creates opportunities to also consider irrational factors in addition to rational ones comprehensively in scientific researches. The modern scientific thought contributes to implementation of these opportunities. Ahangyol is a science of determination of ways to achieve harmony in any sphere and of creation of special methods to make progress in these ways through assistance of the modern science. Methods of the System Theory, Mathematics, IT, Astronomy, Physics, Biology, Sociology, Statistics and etc. are more extensively applied. Information is given on some of these methods. Moreover, the Science of Ahangyol, which is a new philosophical worldview and a new paradigm contributes to clarification of metaphysic views considerably and discovery of the scientific potential of religious books.

Sign in / Sign up

Export Citation Format

Share Document