Calcium Phosphate Hybridized with Human Semitendinosus and Gracilis Tendon Grafts

2007 ◽  
Vol 330-332 ◽  
pp. 1117-1120 ◽  
Author(s):  
Shinobu Kobayashi ◽  
Masataka Sakane ◽  
Hirotaka Mutsuzaki ◽  
Hiromi Nakajima ◽  
M. Tanaka ◽  
...  

We hybridized calcium phosphate (CaP) with human semitendinosus and gracilis (ST/G) tendon grafts using an alternate soaking process. To evaluate quantitatively and histologically assess the CaP hybridized human ST/G tendon grafts, we classified them into three groups according to their soaking time – number of soaking cycle: 30 sec – 20 cycles (Group A), 1 min – 15 cycles (Group B), 3 min – 5 cycles (Group C). The tendon grafts were divided into three parts: tibial end (TE), femoral end (FE) and intra-articular (IA) portion. TE was secured using the Krackow technique with No. 2 nonabsorbable sutures, and an Endobutton-CL (Smith & Nephew, USA) was passed through the looped FE, as performed clinically. Then, the IA portion was covered with the sleeve of a rubber glove to prevent CaP hybridization. More soaking cycles induced greater deposition of CaP in the tendon grafts when the total soaking time was the same. Covering the IA portion with a rubber sleeve prevented of CaP deposition. A large amount of CaP in TE was deposited because suture holes increased the total contact area with the solutions.

2013 ◽  
Vol 56 (3) ◽  
pp. 97-103 ◽  
Author(s):  
Prabath Singh ◽  
Joseph Paul ◽  
Abdul Aziz Al-Khuraif ◽  
Sajith Vellappally ◽  
Hassan Suliman Halawany ◽  
...  

Objectives: The purpose of this study was to evaluate the in vitro sealing ability of three repair materials. Mineral trioxide aggregate (MTA; Group A), calcium phosphate cement (CPC; Group B), and light cured glass ionomer cement (GIC; Group C) when used to repair the perforation created in the pulpal floor of fifty extracted human permanent molars. Materials and methods: Preparation of access openings and furcation perforations were done, and the teeth divided into five experimental groups (A, B, C) including two controls (D, E) with ten samples in each group randomly. Following the repair procedure, the pulp chambers and access openings were filled with composite resin and immersed in 2% methylene blue solution for 48 hours. The teeth were sectioned longitudinally and the linear dye penetration measured under a stereo­microscope. Results: The comparison of the linear length of micro-leakage (mm) among the experimental groups revealed no significant difference (p = 0.332). On calculating the percentage of depth of leakage to the total length of the perforation, it was observed that the mean leakage was 35.5% in Group A, 53.6% in Group B and the highest, 87.5% in Group C. The mean of leakage percentage was statistically significant by Kruskal-Wallis test (p = 0.003). The results indicated that the dye penetration used as furcation perforation repair material was least with mineral trioxide aggregate. Comparing the depth of penetration of dye, 50% of the Group A samples showed less than 25% of depth penetration. While 40% of Group B cases had more than 50% dye penetration. In our study, all Group C teeth had ≥ 50% dye penetration. Conclusions: The present study indicated that GIC had the greatest dye penetration followed by CPC and MTA. Mineral trioxide aggregate and calcium phosphate cement had comparatively better sealing ability than glass ionomer cement.


2021 ◽  
Vol 13 ◽  
pp. 2-6
Author(s):  
Deeksha Jha

BACKGROUND: Studies have documented the importance of alkaline pH for deposition of calcium phosphate, thereby aiding plaque mineralization. The ureolytic pH response promotes calculus formation by increasing the saturation level of calcium phosphate in plaque fluid. Therefore, AIM - A study was conducted to assess the effect of salivary pH and urea level in mild moderate and heavy calculus formers. METHODS: Group A- Mild calculus formers Group B- Moderate calculus formers Group C- Heavy calculus formers 10 subjects were assigned to each group. Salivary pH was recorded by using pH indicator strips as soon as saliva was collected in a sterile container. Thereafter, biochemical estimation of salivary urea was performed. RESULT-: There was no statistically significant difference in pH levels between Group A and Group B (p=0.97) but significant difference between Group A and Group C (p= 0.01).There was a highly significant difference between Group A and Group C in urea levels .(p<0.001). CONCLUSION: Salivary urea has a major influence on calculus formation, and though evidence suggests that pH has a role in calculus formation, the present study did not show any significant relation between pH and calculus formation.


2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Shaymaa M. Nagi ◽  
Shahinaz H. Nabil ◽  
Mohamed H. Zaazou

Abstract Background Using remineralizing agents during and after bleaching procedures could maintain enamel surface, decreasing its erosive susceptibility. This study evaluated the efficacy of an in-office and at-home bleaching materials with amorphous calcium phosphate (ACP) on the susceptibility of the enamel to erosion. Forty-five bovine specimens were separated into three groups. Group A: no bleaching (control). Group B: Zoom 2 bleaching followed by ACP application. Group C: Nite White ACP. All specimens in all groups were subjected to an erosive challenge for five days. Using Energy Dispersive X-ray all specimens were subjected to elemental analysis and were examined for morphological changes under Scanning Electron Microscope (SEM). Results Group B showed the highest mean calcium, phosphorus and sodium weight percent (wt%). Both Groups B and C showed statistically significant difference. While, there was no statistically significant difference between both Groups A and B.The sodium mean wt% showed a statistically significant difference in all groups. The magnesium mean wt% showed no statistically significant difference in all groups. SEM photomicrograph revealed hollowing of prism cores with intact prisms peripheries for group A. Group B showed relativity smooth surface with minor pores, while Group C showed an increase in the depth of surface irregularities and depressions. Conclusions Enamel susceptibility to erosion after bleaching procedure was material dependent. Zoom 2 bleaching agent followed by ACP application did not increase the susceptibility of the enamel surface to erosion. Adding ACP to Nite White bleaching agent had no benefit on reducing enamel susceptibility to erosion.


2012 ◽  
Vol 19 (Number 2) ◽  
pp. 3-6
Author(s):  
N Y Mili ◽  
Md. E Hoque ◽  
S Akhter ◽  
N S Lovely

Since the early 1970s. calcium phosphate (Ca-P) index has been regarded as a risk factor for extra skeletal calcification. tumoral calcinosis and increased cardiovascular event and death. The general consensus was not to exceed 70 ing2h1Lt (5.6 unno1/1,2) in chronic kidney disease. The present study was done to find out the Ca - P index in different stages of (CKB) patients to assess the risks of the patients which can be understood and be negotiated. In this study 100 of previously diagnosed chronic kidney disease patients of different stages as CKD stage Ill. IV and stage V were included. Subjects were divided into three groups according to staging of chronic kidney disease : group A (stage 111) were 34 patients, group 8 (Stage IV) were 36 and group C (Stage V) were 30 patients. Mean serum inorganic phosphate level was in group A .5.41 + 2.49. group 8 8.17 + 3.63 and in group C 10.50 + 3.06. Mean serum Calcium level in three groups were in group-A 8.36± 0.74. group- B 8.10± 0.75 and in group- C was 7.43± 1.27 ). Ca - P index was calculated by multiplying the serum calcium and phosphate level. Mean Ca-P index was in group-A 49.39+ 22.95. group B-67.93+ 31.2 and in group-C 90.76+ 24.82. Statistical analysis was done betWeen these groups and it was signifimuly higher in group B than group A ( p< 0.06. group A is group 8). in group C than group A ( p< 0.00. group A vs group C) and in ,group C than group ft ( p< .002. group B vs group C). It was found that as the renal function deteriorates gradually the Ca P index increases and it is highly significantly higher in CKD — V patient than other stages.


Author(s):  
Shinobu Kobayashi ◽  
Masataka Sakane ◽  
Hirotaka Mutsuzaki ◽  
Hiromi Nakajima ◽  
Junzo Tanaka ◽  
...  

2021 ◽  
Vol 11 (19) ◽  
pp. 8794
Author(s):  
Yen-Nan Lin ◽  
Jun Wang ◽  
Yu Su ◽  
I-Lin Wang

Background: The purpose of this study was to explore the gaze behavior of tennis players with different skill levels when receiving serves through eye movement information. Methods: The skill level was divided into group A (experts, with more than 10 years of playing experience) and group B (novices, with less than 2 years of playing experience). We compared the differences in gaze behavior between groups A and B at the head-shoulder, trunk-hips, arm-hand, leg-foot, racket, ball, and racket-ball contact area seven positions using the Eye-gaze Response Interface Computer Aid (ERICA) device. Data were analyzed using two-way ANOVA. Results: Compared with the novices, the experts have more gaze time in the head–shoulders, rack, and ball when serving forehand (p < 0.01). The experts also have more gaze time on the head–shoulders, trunk–hips, racket, ball, and racket–ball contact area when serving backhand (p < 0.05). Conclusions: Expert athletes have a longer stare time for a specific position, which mainly determines the direction of the ball. Tennis coaches can increase the gaze time for these four positions and improve tennis players’ ability to predict the direction of the ball.


2021 ◽  
Vol 11 (9) ◽  
pp. 1213
Author(s):  
Ligia Rusu ◽  
Elvira Paun ◽  
Mihnea Ion Marin ◽  
Jude Hemanth ◽  
Mihai Robert Rusu ◽  
...  

Background: Evaluation of plantar pressure in stroke patients is a parameter that could be used for monitoring and comparing how the timing of starting a rehabilitation program effects patient improvement. Methods: We performed the following clinical and functional evaluations: initial moment (T1), intermediate (T2), and final evaluation at one year (T3). At T1 we studied 100 stroke patients in two groups, A and B (each 50 patients). The first group, A, started rehabilitation in the first three months after having a stroke, and group B started after three months from the time of stroke. Due to the impediments observed during rehabilitation, we made biomechanic evaluation for two lots, I and II (each 25 patients). Assessment of the patient was carried out by clinical (neurologic examination), functional (using the Tinetti Functional Gait Assessment Test for classifying the gait), and biomechanical evaluation (maximal plantar pressure (Pmax), contact area (CA), and pressure distribution (COP)). Results: The Tinetti scale for gait had the following scores: for group A, from 1.34 at the initial moment (T1) to 10.64 at final evaluation (T3), and for group B, 3.08 at initial moment (T1) to 9 at final evaluation (T3). Distribution of COP in the left hemiparesis was uneven at T1 but evolved after rehabilitation. The right hemiparesis had uniform COP distribution even at T1, explained by motor dominance on the right side. CA and Pmax for lot I increased more than 100%, meaning that there is a possibility for favorable improvement if the patients start the rehabilitation program in the first three months after stroke. For lot II, increases of the parameters were less than lot I. Discussions: The recovery potential is higher for patients with right hemiparesis. Biomechanic evaluation showed diversity regarding compensatory mechanisms for the paretic and nonparetic lower limb. Conclusions: CA and Pmax are relevant assessments for evaluating the effects on timing of starting a rehabilitation program after a stroke.


2005 ◽  
Vol 284-286 ◽  
pp. 289-292 ◽  
Author(s):  
Ji Yong Chen ◽  
You Rong Duan ◽  
Xing Dong Zhang

Two sets of porous biphasic calcium phosphate ceramics (BCP) were prepared for dynamic SBF experiment: porous BCP with micropores on the walls of macropores( set A) and porous BCP with dense walls of macropores (set B). Apatite layer could only formed on the macropore walls with micropores. Four groups of specimens were prepared for animal experiments. Group A was porous BCP ceramics with micropores on the walls of macropores; group B was porous BCP with dense walls of macropores; group C was porous BCP ceramics with apatite layers formed by static SBF[2]on their surfaces; group D was porous BCP ceramics with apatite layers formed by dynamic SBF on their walls of macropores. The result of dynamic SBF animal experiments showed that microstructure of BCP played an important role in the bone-like apatite formation and osteoinductiion in biomaterials. Apatite formation may be the prerequisite of osteoinductive formation of new bone.


Author(s):  
Taber A. Ba-Omar ◽  
Philip F. Prentis

We have recently carried out a study of spermiogenic differentiation in two geographically isolated populations of Aphanius dispar (freshwater teleost), with a view to ascertaining variation at the ultrastructural level. The sampling areas were the Jebel Al Akhdar in the north (Group A) and the Dhofar region (Group B) in the south. Specimens from each group were collected, the testes removed, fixed in Karnovsky solution, post fixed in OsO, en bloc stained with uranyl acetate and then routinely processed to Agar 100 resin, semi and ultrathin sections were prepared for study.


VASA ◽  
2015 ◽  
Vol 44 (3) ◽  
pp. 0220-0228 ◽  
Author(s):  
Marion Vircoulon ◽  
Carine Boulon ◽  
Ileana Desormais ◽  
Philippe Lacroix ◽  
Victor Aboyans ◽  
...  

Background: We compared one-year amputation and survival rates in patients fulfilling 1991 European consensus critical limb ischaemia (CLI) definition to those clas, sified as CLI by TASC II but not European consensus (EC) definition. Patients and methods: Patients were selected from the COPART cohort of hospitalized patients with peripheral occlusive arterial disease suffering from lower extremity rest pain or ulcer and who completed one-year follow-up. Ankle and toe systolic pressures and transcutaneous oxygen pressure were measured. The patients were classified into two groups: those who could benefit from revascularization and those who could not (medical group). Within these groups, patients were separated into those who had CLI according to the European consensus definition (EC + TASC II: group A if revascularization, group C if medical treatment) and those who had no CLI by the European definition but who had CLI according to the TASC II definition (TASC: group B if revascularization and D if medical treatment). Results: 471 patients were included in the study (236 in the surgical group, 235 in the medical group). There was no difference according to the CLI definition for survival or cardiovascular event-free survival. However, major amputations were more frequent in group A than in group B (25 vs 12 %, p = 0.046) and in group C than in group D (38 vs 20 %, p = 0.004). Conclusions: Major amputation is twice as frequent in patients with CLI according to the historical European consensus definition than in those classified to the TASC II definition but not the EC. Caution is required when comparing results of recent series to historical controls. The TASC II definition of CLI is too wide to compare patients from clinical trials so we suggest separating these patients into two different stages: permanent (TASC II but not EC definition) and critical ischaemia (TASC II and EC definition).


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