Effect of Collagen Coating on Lipid Accumulation and Differentiation on 3T3-L1 Preadipocyte Treated with Conjugated Linoleic Acid

The purpose of this study is to make use of trans10,cis12 CLA (t-CLA) that has potential for proliferation and differentiation to form adipocyte on the collagen-coated surface. Results provided evidences of good adhesion, growth, viability, and differentiation of adipocyte on collagen-coated surface compared with non-coated surface. Also, the results showed that mouse 3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface, and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo full maturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA was higher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. These results suggest that collagen coating may provide a promising approach to develop a new adipocyte replacement strategy using CLA.

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Traditional Herbal Formula Oyaksungi-San Inhibits Adipogenesis in 3T3-L1 Adipocytes

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/949461 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Sae-Rom Yoo ◽

Chang-Seob Seo ◽

Hyeun-Kyoo Shin ◽

Soo-Jin Jeong

Keyword(s):

Binding Protein ◽

Peroxisome Proliferator ◽

Herbal Formula ◽

Related Factors ◽

Peroxisome Proliferator Activated Receptor ◽

Fatty Acid Binding ◽

Gpdh Activity ◽

Protein Levels ◽

Intracellular Lipid Accumulation ◽

Glycerol 3 Phosphate Dehydrogenase

Background. Oyaksungi-san (OYSGS) is a herbal formula that has been used for treating cardiovascular diseases in traditional Asian medicine. Here, we investigated the antiadipogenic effect of OYSGS extract in 3T3-L1 adipose cells.Methods. 3T3-L1 preadipocytes were differentiated into adipocytes with or without OYSGS. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, leptin production, mRNA, and protein levels of adipogenesis-related factors.Results. OYSGS extract dramatically inhibited intracellular lipid accumulation in the differentiated adipocytes. It also significantly suppressed the (GPDH) activity, triglyceride (TG) content, and leptin production by reducing the expression of adipogenesis-related genes including lipoprotein lipase, fatty acid binding protein 4, CCAAT/enhancer-binding protein-alpha (C/EBP-α), and peroxisome proliferator-activated receptor gamma (PPAR-γ). Furthermore, OYSGS clearly enhanced phosphorylation of AMP-activated protein kinase (AMPK) as well as its substrate acetyl CoA (ACC) carboxylase.Conclusions. Our results demonstrate that OYSGS negatively controls TG accumulation in 3T3-L1 adipocytes. We suggest antiadipogenic activity of OYSGS and its potential benefit in preventing obesity.

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Platelets selectively enhance lymphocyte adhesion on subendothelial matrix under arterial flow conditions

Thrombosis and Haemostasis ◽

10.1160/th12-02-0064 ◽

2012 ◽

Vol 108 (08) ◽

pp. 328-337 ◽

Cited By ~ 16

Author(s):

Maria Ersoy ◽

Paul Hjemdahl ◽

Naphtali Savion ◽

David Varon ◽

Galia Spectre ◽

...

Keyword(s):

Cell Adhesion ◽

B Cells ◽

Nk Cells ◽

Platelet Activation ◽

Platelet Adhesion ◽

Nk Cell ◽

Lymphocyte Adhesion ◽

Subendothelial Matrix ◽

Coated Surface ◽

Coated Surfaces

SummaryPlatelet adhesion at sites of cardiovascular injury may facilitate leukocyte deposition. We asked if and how platelets enhance lymphocyte adhesion on different subendothelial matrix protein (SEMP)-coated surface at arterial shear stress. Hirudinised whole blood was subjected to an arterial shear rate (500 s−1) in a Cone and Plate(let) analyser (CPA) for 5 minutes using plates coated with bovine serum albumin (BSA), collagen, fibrinogen, von Willebrand factor (vWF), or fibronectin. Platelet and lymphocyte adhesion were monitored by CPA and flow cytometry. Exposure of blood to collagen, fibrinogen, and vWF-coated surfaces induced platelet activation. The most marked effect was seen with collagen-coating, which markedly enhanced the adhesion of all lymphocyte subpopulations compared to BSA-coating. Fibrinogen-coating supported both T and NK cell adhesion, while vWF-coated surface only enhanced NK cell deposition. In contrast, fibronectin enhanced neither platelet activation nor lymphocyte adhesion. Moreover, platelets preferentially facilitated adhesion of large CD4+ and CD8+ T cells and NK cells, and of small B cells. Enhanced cell adhesion of larger lymphocytes was associated with elevated platelet conjugation and higher lymphocyte expression of PSGL-1, Mac-1, and CD40L. The enhancement of lymphocyte adhesion was totally platelet-dependent, and was abolished in platelet-depleted blood. Moreover, blockade of the platelet adhesion molecules P-selectin, GPIIb/IIIa, and CD40L attenuated platelet-dependent lymphocyte deposition. In conclusion, platelets support lymphocyte adhesion on SEMP-coated surfaces under arterial shear. The enhancement is selective for large T and NK cells and small B cells.

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Proliferation and differentiation of human adipocyte precursor cells: Differences between the preperitoneal and subcutaneous compartments

Journal of Cellular Biochemistry ◽

10.1002/jcb.22753 ◽

2010 ◽

Vol 111 (3) ◽

pp. 659-664 ◽

Cited By ~ 7

Author(s):

M. Fernández ◽

M.J. Acuña ◽

M. Reyes ◽

D. Olivares ◽

S. Hirsch ◽

...

Keyword(s):

Precursor Cells ◽

Human Adipocyte ◽

Proliferation And Differentiation ◽

Adipocyte Precursor

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Controlling Stem Cell Adhesion, Proliferation, and Differentiation with Layer-by-Layer Films

Layer-by-Layer Films for Biomedical Applications ◽

10.1002/9783527675869.ch6 ◽

2015 ◽

pp. 103-130

Author(s):

Stewart Wales ◽

Guak-Kim Tan ◽

Justin J. Cooper-White

Keyword(s):

Stem Cell ◽

Cell Adhesion ◽

Layer By Layer ◽

Proliferation And Differentiation

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Conjugated linoleic acid increased C-reactive protein in human subjects

British Journal Of Nutrition ◽

10.1079/bjn20041419 ◽

2005 ◽

Vol 94 (5) ◽

pp. 791-795 ◽

Cited By ~ 58

Author(s):

Annika Smedman ◽

Samar Basu ◽

Stefan Jovinge ◽

Gunilla Nordin Fredrikson ◽

Bengt Vessby

Keyword(s):

Cell Adhesion ◽

Linoleic Acid ◽

Cell Adhesion Molecule ◽

Human Subjects ◽

Vascular Cell Adhesion ◽

C Reactive Protein ◽

Vascular Cell ◽

Reactive Protein ◽

Tnf Α ◽

Cell Adhesion Molecule 1

We previously showed that conjugated linoleic acid (CLA) increases 15-keto-dihydro-prostaglandin F2α, a marker for cyclooxygenase-mediated lipid peroxidation and thus an indicator of cyclooxygenase-mediated inflammation. The aim of the present study was to investigate the effects of CLA on other indicators of inflammation in human subjects, including C-reactive protein, TNF-α, TNF-α receptors 1 and 2, and vascular cell adhesion molecule-1. In a double-blind, placebo-controlled study, fifty-three human subjects were supplemented with a mixture (4·2g/d) of the isomers cis-9,trans-11 CLA and trans-10,cis-12 CLA or control oil for 3 months. CLA supplementation increased levels of C-reactive protein (P=0·003) compared with the control group. However, no changes in TNF-α, TNF-α receptors 1 and 2, and vascular cell adhesion molecule-1 were detected.

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The leucocyte β2 (CD18) integrins: the structure, functional regulation and signalling properties

Bioscience Reports ◽

10.1042/bsr20110101 ◽

2012 ◽

Vol 32 (3) ◽

pp. 241-269 ◽

Cited By ~ 82

Author(s):

Suet-Mien Tan

Keyword(s):

Wound Healing ◽

Cell Adhesion ◽

Immune System ◽

Adhesion Molecules ◽

Cell Adhesion Molecules ◽

Biological Processes ◽

Motile Cells ◽

Proliferation And Differentiation ◽

Functional Regulation ◽

Β2 Integrins

Leucocytes are highly motile cells. Their ability to migrate into tissues and organs is dependent on cell adhesion molecules. The integrins are a family of heterodimeric transmembrane cell adhesion molecules that are also signalling receptors. They are involved in many biological processes, including the development of metazoans, immunity, haemostasis, wound healing and cell survival, proliferation and differentiation. The leucocyte-restricted β2 integrins comprise four members, namely αLβ2, αMβ2, αXβ2 and αDβ2, which are required for a functional immune system. In this paper, the structure, functional regulation and signalling properties of these integrins are reviewed.

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Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro

Bioscience Reports ◽

10.1042/bsr20160049 ◽

2016 ◽

Vol 36 (6) ◽

Cited By ~ 2

Author(s):

Bohan Cheng ◽

Mengqi Wu ◽

Songsong Xu ◽

Xinyang Zhang ◽

Yuxiang Wang ◽

...

Keyword(s):

Fatty Acid Binding Protein ◽

Peroxisome Proliferator ◽

Induction Method ◽

Preadipocyte Differentiation ◽

Peroxisome Proliferator Activated Receptor ◽

Fatty Acid Binding ◽

Gpdh Activity ◽

Acid Binding Protein ◽

Glycerol 3 Phosphate Dehydrogenase

Chicken preadipocytes cultured in cocktail supplement with rosiglitazone resulted in a marked increase in lipid droplet accumulation, glycerol-3-phosphate dehydrogenase (GPDH) activity and mRNA expression of adipocyte fatty acid-binding protein (aP2), G0/G1 switch gene 2 (G0S2), peroxisome proliferator-activated receptor γ (PPARγ) and lipolysis. The present study provides a novel induction method for in vitro chicken preadipocyte differentiation.

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